LOCUS       CP057490                2590 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB24-C19 plasmid pRHB24-C19_4, complete
            sequence.
ACCESSION   CP057490
VERSION     CP057490.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148231
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH15 T2-C19
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 135.114677449927x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:52:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,916
            CDSs (total)                      :: 4,795
            Genes (coding)                    :: 4,698
            CDSs (with protein)               :: 4,698
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 43 of 97
            Pseudo Genes (incomplete)         :: 49 of 97
            Pseudo Genes (internal stop)      :: 19 of 97
            Pseudo Genes (multiple problems)  :: 14 of 97
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB24-C19"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB24-C19_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(2070..2590,1..469)
                     /locus_tag="HVY14_24575"
     CDS             join(2070..2590,1..469)
                     /locus_tag="HVY14_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QMI21491.1"
                     /translation="MQNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYEHYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESD"
                     /name="protein rep"
     gene            20..469
                     /locus_tag="HVY14_24580"
     CDS             20..469
                     /locus_tag="HVY14_24580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QMI21490.1"
                     /translation="MLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKDGESL
                     ACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLKLDQ
                     ETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESD"
                     /name="protein rep"
     Typing          473..556
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.941"
                     /coverage="93.0"
                     /evalue="1.22e-26"
     Typing          682..937
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.186"
                     /coverage="89.0"
                     /evalue="1.23e-106"
     Typing          complement(1417..1498)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="93.0"
                     /evalue="3.4000000000000004e-27"
     Typing          complement(196..387)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.446"
                     /coverage="100.0"
                     /evalue="2.86e-91"
ORIGIN
        1 gatgatggct cctcctggca tgctaaatgg gaaaagttac gtcaggcatg aacgctgggt
       61 tgagctatgg cgggagtgcc tgcgggttga ttatgacccg aatgttgatg tccgggcagt
      121 gaaaccccgc aagccgaagg atggcgagtc cttagcctgc gcgaccgctg aactggtgcg
      181 cggcgcagtg gccgaaaccc tgaaatattc cacgaagccg gctgacatgg tggccgatcc
      241 tgaatggttc ctcgaactga ctcgccagac gcacaagcgc cgctttgtgg cgaccggcgg
      301 cgcactcaaa gatgtgctga aactcgatca ggaaaccgac gccgacatgg tgatcggaga
      361 tgatatttcc gagggcgacg atgacggttc acgcatcgcg ttcgaatgga aaaccgaggt
      421 cagaaagtac cgccgctcac cgacgaaaga taaaactgaa tcagactagc agggtgtcgg
      481 ggcgcagccc tgaccaggtg gtaattgtga tagcgtcgcg tgtgacggta ttacaattac
      541 acatcctgtc ctgtttcaac tgataaataa tccctgtgca gtacagccgc tgttgcataa
      601 aatttcgctc tgcgtcgagt gtttcttaat caagaattcc gctgatactc cggaaaaacg
      661 cgagtctgtt ctcatgcctg aaatgcccac accccacgca aaaacaattt tttgctgatt
      721 tttctttata aatagagagt tatgacaaat tagtttctct tgctctcttt gtgatattta
      781 aaaaagcggt gtcggcgcgg tgttgtagct gcgccgacac cgctttgtag gggtggtact
      841 gactattttc ataaaaaaca taattttata ttaggggtac tgctagcggc gcggtgtgtt
      901 tttttacagg acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga
      961 gtcagggatt cacgaattga aaatgtccga aaatatgtct catgggcact gtatgcgccc
     1021 aaaccgatcg ccattcagtt tcatgcatag tcatgcaacg cgctgaaaac gttcctgacg
     1081 catttttccg gtttaccctg gggaaaacat ccctttttgc ggtgttcgcg gcagaatcgc
     1141 gttcagcgcg ttttagcggt gcgcgtaaca agtccttaca ccacgcgccc caccccgcgg
     1201 tcgttcccgt tccgtacagc catcgccacc gacgcgccaa attctggcga ccggacagca
     1261 aatcattttg ccgtttttgt gccgcagccg ttccgcgtga tcgacaaact ggcagtcagg
     1321 gaatctcacg aagtgcgcac ttatacaaac ttcacttcgt ttcgtttgtc gtgcggtctg
     1381 cgacgctcaa aaaaccgaga aacggcggga attttcggga caggatgtgt aattgtaata
     1441 ccgtcacacg cgacgctatc acaattacca cctggtcagg gctgcgcccc gacaccccgc
     1501 caacgcctca cggcccgcga aaaaattccg cgtaccgctc accgttcccg aactcgtcaa
     1561 acagaaaatc gcggggcccc gcgcctctgg cgtgggttcc gattttgtga ttgacgacgg
     1621 cacggggaaa acactgggga tggtggttgg tgtcacggag tgaggccaac cacctgcaag
     1681 acggtttttt gttgttcaca aaatcggggt gaaagtctgt ggatgagatg ggtgtaagtg
     1741 ggtggggttg ggaggttttt ggttactgaa aatccagctc aaaattcaag caaatctcgc
     1801 gcgtgatcct tgtatttata cttaagggat aactctagga ttcaaattcg cctccagccc
     1861 ttgtgccgca aggcctgagg ggggatttgc cggaaaatgg ttttggagtt attctgtcgg
     1921 gagtggttgc gttaaaagtg acccccgcaa cgacgggcat cgttcggggg tgtgaccacc
     1981 tgcagaaact ggattcatga tgagagcaac aggtgatatg gcaatgataa cacgtcaatc
     2041 gagccatcgc ccagtatggg gcggtgtgca tgcaaaaccc taaactccag tacctcactg
     2101 attattcccc ctctgatgcg ccctgggacg cacacagaag cgtttctgac gacgtgggag
     2161 ggatttacct gcttgcggcc gaatatgagc actacggggc tcgtatggct tcctgtggcg
     2221 gtttactgcg tttcgggtgg tctaccctca aggaaaccgg agaaacgcgt ctgaggctgc
     2281 gtgaggcaca tttctgccgt gtccggcact gtcctgtctg ccagtggagg cgctccctga
     2341 tgtggcaagc ccgtttctac cagtccctgc cgcgtatcgt ggcggattat cccgatgccc
     2401 gctggatgtt cctgaccctg accgtgcgca actgtgcgat tggggaactg ggcgagatgc
     2461 tgaaccggat gaatgcagct tttcagcgta tgaaggttcg caaggagttc ctgcctgttc
     2521 agggctggat ccgcacaacg gaagttaccc gcggcagcga cggcagcgca cacccgcact
     2581 tccatacctt
//
