LOCUS       CP057457                6811 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB26-C12 plasmid pRHB26-C12_3, complete
            sequence.
ACCESSION   CP057457
VERSION     CP057457.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148248
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6811)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH09 T2-C12
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 134.925672976917x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:17:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,909
            CDSs (total)                      :: 4,788
            Genes (coding)                    :: 4,705
            CDSs (with protein)               :: 4,705
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 42 of 83
            Pseudo Genes (incomplete)         :: 28 of 83
            Pseudo Genes (internal stop)      :: 30 of 83
            Pseudo Genes (multiple problems)  :: 16 of 83
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB26-C12"
                     /isolation_source="Pooled cattle faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB26-C12_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(5906..6811,1..258)
                     /gene="cna"
                     /locus_tag="HVY31_24495"
     CDS             join(5906..6811,1..258)
                     /gene="cna"
                     /locus_tag="HVY31_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin colicin N"
                     /protein_id="QMH57028.1"
                     /translation="MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWS
                     WSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGASAKVGEITITPDNS
                     KPGRYISSNPEYSLLAKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNW
                     KGPKYNNKLVKRFVSQFLLFRKEEKEKNEKEALLKASELVSGMGDKLGEYLGVKYKNVA
                     KEVANDIKNFHGRNIRSYNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAKDMANK
                     IGNLGKAFKVADLAIKVEKIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAISLFGA
                     VLSFLPISGLAVTALGVIGIMTISYLSSFIDANRVSNINNIISSVIR"
                     /name="cna"
     gene            complement(267..791)
                     /gene="cni"
                     /locus_tag="HVY31_24500"
     CDS             complement(267..791)
                     /gene="cni"
                     /locus_tag="HVY31_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061352107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin N immunity protein"
                     /protein_id="QMH57029.1"
                     /translation="MDIKDRNKISKKISFSLLLLLSPFALIFFSYNNAPIPLLEKIIAY
                     LSLPGFHSLNNPPLSEAFNLYVHTAPLAAISLFIFTHKELELKPKSSPLRALKILTPFT
                     ILYISMIYCFLLTDTELTLSSKTFVLMSKNDLFLSFFYITLYIGIYIFTYLYFWFLIGT
                     YKLFTRGGILP"
                     /name="cni"
     gene            819..956
                     /locus_tag="HVY31_24505"
     CDS             819..956
                     /locus_tag="HVY31_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMH57030.1"
                     /translation="MCGKILLILFFIMTLSACQVNHIRDVKGGTVAPSSSSRLTGVEIK
                     "
                     /name="colicin release lysis protein"
     gene            complement(1976..2167)
                     /locus_tag="HVY31_24510"
     CDS             complement(1976..2167)
                     /locus_tag="HVY31_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMH57031.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2231..2554
                     /locus_tag="HVY31_24515"
     CDS             2231..2554
                     /locus_tag="HVY31_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMH57037.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2496..3254
                     /locus_tag="HVY31_24520"
                     /pseudo=""
     CDS             2496..3254
                     /locus_tag="HVY31_24520"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3232..3750
                     /locus_tag="HVY31_24525"
     CDS             3232..3750
                     /locus_tag="HVY31_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMH57032.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            3757..3990
                     /gene="mbeD"
                     /locus_tag="HVY31_24530"
     CDS             3757..3990
                     /gene="mbeD"
                     /locus_tag="HVY31_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMH57033.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(4346..4516)
                     /locus_tag="HVY31_24535"
     CDS             complement(4346..4516)
                     /locus_tag="HVY31_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMH57034.1"
                     /translation="MSKAPIGTTASTGQKCPESGVWKVVGTPSTTAPIAKGNVMPPYNG
                     KAVTWKLTQYA"
                     /name="hypothetical protein"
     gene            complement(4710..5129)
                     /gene="exc2"
                     /locus_tag="HVY31_24540"
     CDS             complement(4710..5129)
                     /gene="exc2"
                     /locus_tag="HVY31_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMH57035.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSQSDVQQRIQHFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(5210..5647)
                     /locus_tag="HVY31_24545"
     CDS             complement(5210..5647)
                     /locus_tag="HVY31_24545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMH57036.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGFVSYRTSQDGRR
                     EFETSELIRAYGELKQNETPERHSAGQAENPHDQPTERILRELNELKQCLTLMLEDKQA
                     QDMDRKRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     Typing          1804..1891
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(1235..1780)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.526"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2545..4094
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.183"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaaaaagca ttgagggata caatactggc aactggggac ctctcctgtt ggaggttgaa
       61 tcatggatca ttggtggcgt tgttgctgga gttgctatta gtttattcgg ggctgtgttg
      121 agttttctcc caatctctgg acttgcagtt actgcgttgg gggtaatagg aataatgacg
      181 attagttact tgtcatcttt catagatgca aatcgagttt cgaatataaa taacattata
      241 tctagtgtta ttcgatgata ttagtgtcag gggagtattc ctcccctggt aaatagctta
      301 tatgttccta taaggaacca aaagtacaaa tatgtgaata tatatatccc aatatatagt
      361 gttatataga aaaaagacaa aaacagatcg ttttttgaca ttaatacaaa tgtttttgat
      421 gacaaggtta gttctgtgtc agttagcaag aaacagtata tcatggatat ataaagaata
      481 gtgaaaggag ttaatatctt tagtgcccgc agaggtgacg actttggttt taactctaat
      541 tctttgtgtg tgaatatgaa taagctgatt gcagctaaag gggctgtatg aacatagaga
      601 ttgaatgctt cgcttagggg cgggttgttt aatgaatgaa atcctggtag ggataggtat
      661 gcgatgattt tttcgaggag tggtattggt gcattattat aactgaaaaa tattaatgcg
      721 aatggggaaa gtaagagcag aagactgaat gatatttttt ttgatatctt atttctgtct
      781 tttatatcca tgttgctttc tttggatggt tgaaaattat gtgtgggaaa attttattaa
      841 tcttgttttt tatcatgaca cttagtgcat gtcaggtcaa tcacatccgt gatgtgaaag
      901 gagggactgt tgctccatca tcatcatcca gactaacagg ggttgaaatt aagtaaaaga
      961 tcaaaggatc ctcttgagat cctttttttc tacgcgtaat ctgctgcctg taaacgaaaa
     1021 aaccgcctgg ggaggcggtt tagtcgaagg ttaagtcagt tggggactgc ttaaccgagg
     1081 taactggctt cagcagagcg cagataccaa atactgtcct ttcagtgtag ccgcagttgg
     1141 gccatcactt caagaactct gtaagcatct ggataaatct cgctctgcta atccggttac
     1201 cagtggctgc tgccagtggc gttaaggcgt gtcttaccgg gttggactca agacgatagt
     1261 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1321 agcgaacgac ctacaccaag ccgagatacc aacagcgtga gctatgagaa agcgccacgc
     1381 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1441 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1501 acctctgact tgagtgtcgg tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1561 acgccatcgg cgcggccttt ttccggttcc tggtcttttg ctcacatgtt ctttccggcc
     1621 ttatcccctg attctgtgga taaccgtatt accgtctttg agtgagctga taccgctcgc
     1681 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     1741 cggtatgttc tccttacgca tctgtgcggc atttcacacc cggcatggcg tgcttttcat
     1801 acaatccgca ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     1861 gggtcagggc tgcgccccga cacccgctaa aacctgctga cgcgccctga cgggcttgtc
     1921 tgctcccggc atccgctcac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1981 ggttttcacc gtcatccccg aaacgtgcga ggcagctgcg gtaaagctca tcggcgtggt
     2041 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttttcca
     2101 gcagcgttaa tgtctggctt ctgataaagc gcgccatgtt aagggcggtt ttttcctgtt
     2161 tagtcactga tgcctccgtg taagggggat ttctgttcat ggggtaatga taccgatgaa
     2221 acgcgagagg atgctcacga tacgggttac tgatgatgaa catgccaggt tacttgaacg
     2281 ctgtgagggt aaacaactgg cagtatggat gcggcgggtc tgcctgggtg agccggttgc
     2341 ccgttccggg aagctgccga cactggcacc gccgttactg cgtcagctgg ccgccatcgg
     2401 gaataacctg aatcagacag cgaggaaggt gaacagcggg cagtggtctt ccggtgaccg
     2461 ggttcaggtg gtggccgcac tgatggccat cggggatgag ctgcgccggc tgcgtctggc
     2521 cgtcagggaa cagggggcgc gggatgatag ttaaatttca tgccagggga aaaggtggtg
     2581 gcagtggtcc ggttgattac ctgctgggga gggagcgtaa ccgcgaaggc gcaacggtgc
     2641 ttcagggtaa tccggaagaa gtccgggaac tcatcgatgc cacgccattt gcgaagaaat
     2701 acacgtccgg tgttctgtcg ttcgcggaga aggagctgcc gccgggagga cgtgaaaaag
     2761 tgatggcgag ctttgagcgt gtactgatgc ccggtctcga aaaaaatcag tacagcatcc
     2821 tgtgggtgga gcaccaggac aagggacggc ttgagctgaa ttttgtcatt ccgaacatgg
     2881 agctacagac cggaaaacgc ctccagccgt actacgaccg cgcagacagg cctagaattg
     2941 atgcctggca gacgctggtt aatcaccatt acgggctgca tgacccgaac gccccggaga
     3001 accgcaggac gctgacactc tctgataatc tgccggaaac gaaacaggcg attgctgagg
     3061 gcgttacacg cgggatagac gcgctttacc atgccggaga gataaaaggc cgtcaggatg
     3121 tgattcaggc gctcacagag gcggggcttg aagtggtcag ggtgacgcga agcagtatca
     3181 gcattgcaga cccgaacggc gggaggaata tcagactgaa aggagcgttt tatgagcaat
     3241 cttttacaga cgggcgcgga gttcgagaaa aagctgaaag agagagccga atctacagag
     3301 aaaatgctga acgacgagtt caggaagctc ggcgagtctg tcagcgaggc tgtgacctca
     3361 aacgagacga aaatcagaga cgctatagcc ctgttcacag cctcgacaga gaaatcgctg
     3421 gaaaaacacc gggaaggggt gaaagaggcg atgatgcagc acaggaagga cgtgttaaag
     3481 ctggcaggga atacgggcat gatgttactg gggatagtct ttctcctgtt taccgcgagt
     3541 ggcgggacgc tctggtatct tggcggacgg atacagacga acctggaaga aatcaggaag
     3601 caggaagaga cattgcagaa actgaacgcg aagacatggg gcgtggagtt tgtgcaggac
     3661 gggaacagga aattccttgt ccttccgtac gggaaatcag cggaggtgat tccctttcag
     3721 gggaaagagt gggtacatct gaaggagtga cacagagtga cagagctgga aatacatttg
     3781 ctgaacgcct tagagcagct gcaacaggac tatatgcagc ggctgagcga atgggagagc
     3841 gccttcgtgg aattgcagaa gatgttttcg cttacgcaac gggacaacgc gatgctgaac
     3901 gagcgggtca tgcagttgag tcagcaggtg cagcacttga gcgagcagac agaacgcttg
     3961 agccagttat acagcgagaa ctggagataa gagaggaacg gctgatacag gagcgcgaac
     4021 atgcgttatc cctggaacgg gagcgtcagc cggaaataca ggaacgcacg ctggatggtc
     4081 cttcgctggg atggtgaaac catgaaaaat ggcagcttca gtggattaag aggggttaat
     4141 gtggcctgta ccatctggtt gcataggtat tcatgcggtt aaaatttatc aggcgcgatc
     4201 gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt gatcgcaccg
     4261 aacgcgtttt ggcggtgcgt acaattaagg gattatggta aatccaagaa taacgaaaaa
     4321 gcccccggtg ggggcttttg agatatcaag cgtattgggt taatttccaa gttacggctt
     4381 ttccgttata cggaggcatt acattgcctt tggcaattgg tgctgtagtt gaaggcgttc
     4441 ctaccacttt ccaaacaccg ctttcagggc atttttgtcc ggttgaagct gttgtaccaa
     4501 ttggggcttt agacatattg attcctcttg tatgttttat ccgtatgctt gtgtagcaat
     4561 acttatttct ggtttagaaa tcagctttca agcgggctta aaggtcgttt tttcacagat
     4621 gtgattgtgg tcatattttt aacaaagttg tatatgattt tggcgttgta agttttggtt
     4681 ttgttataaa ggacttatgg aaaatcaact tactgcctgc cctcgtagcc atcgagataa
     4741 acagccgaaa tggtgtcagc cagcagcctg gattttttcg gggtgaggtc tttgctgcgg
     4801 taatcggtac ccgcaaattg tgttgtgcct cggagtgagt ttaaaaattc ctgactgtga
     4861 aagtgctgaa tgcgttgctg cacatcactc tgtgattttc ctgtcgcttt atctttagct
     4921 cccatatccc agaactgccg aaaaaaaggc accatcattc gtgttgtgtc ggccttctgg
     4981 gtggaaaagt taggatcaaa accgtcatca gaagcatata cgtaatgccg ggtatgttgt
     5041 tccacgctgc cctttggggt acaggctgtg agagctactg ttgctgttat catggcgata
     5101 agacacagtt tgtttccggt acgtctcatg agtgtctccc taccttcaag tgtttattct
     5161 cggtgagtta ttatccgaag actattttat catcagtctc tgacagcgtt cagcgaccga
     5221 acagcctgga ccagaatccc cgtttctttt ccagttccag tgcctgcctg agctgggcta
     5281 tctcattctg cagctgttcc cgttctgctt cctggcgttt gcgatccata tcctgtgcct
     5341 gtttgtcctc aagcatcagc gtcaggcatt gtttcagctc attcagctcc cggagaatgc
     5401 gttctgtcgg ctgatcatgt ggattttctg cctgtcccgc actgtgcctt tctggtgtct
     5461 cattctgctt taattcgccg tatgcccgga tcagttcact ggtttcaaac tcccgtcgtc
     5521 cgtcttgact ggtacggtaa ctgacaaagc ctgcgcgcat atcacggtaa aactgtgagc
     5581 gcgatctgcc tgtcattttc agtgcgtcac ggagtgtgtg ccatgccata aagtgacagt
     5641 gtcccatagg tgtctcatct catggtttca gtaaaacata atgaggtctg agaacggtaa
     5701 tgtttgtgct ggtttttgtg gtatccggcg ggaatagcac gtggtgtggg tggctgtttt
     5761 tttgatcgtt ttcacaaaaa cgaaaattcc cagggttgac aggaaaaacg cagcggcgta
     5821 attttaaact gtatataaaa ccagtggtta tgtgtacagt atttattttt aacggtttgt
     5881 tttaaaagtc aaagagggat ttattatggg tagtaatggc gcagataatg cacataacaa
     5941 tgcttttggt ggagggaaaa atccgggcat tggtaatacc agtggcgcag gaagtaatgg
     6001 tagtgcatca agtaaccgag gaaattccaa tggatggtca tggagtaata agcctcataa
     6061 aaatgatggc ttccacagtg atggttctta ccatattaca tttcatgggg acaataattc
     6121 aaagcctaaa cctggaggga atagtggaaa tcgaggtaat aacggtgatg gagcgagtgc
     6181 taaggttgga gagataacaa tcacacctga caactcgaaa ccaggtcgtt atatttcgtc
     6241 aaatcctgaa tattcattgt tggcaaaatt aattgatgcg gaatcaatta aaggtacaga
     6301 ggtatatact tttcacacca gaaaaggtca gtatgttaag gttactgttc cagatagtaa
     6361 tattgataaa atgagagttg attatgtgaa ctggaaggga ccgaaatata acaataaact
     6421 tgtgaagagg tttgtgagcc agtttttatt atttaggaag gaagaaaaag aaaaaaatga
     6481 aaaagaagcc ttgctaaagg ctagtgaact tgtgtctggt atgggggata agcttggcga
     6541 gtatcttgga gtaaaatata aaaatgtagc taaggaagtt gccaatgata ttaaaaactt
     6601 ccatggtcgt aatattcgta gctataatga agcaatggct tcacttaata aagtgttagc
     6661 aaatccaaag atgaaagtaa acaaatctga taaggatgcc attgtgaatg cctggaaaca
     6721 ggttaatgct aaggacatgg ctaataagat tggtaatctt ggcaaggcat ttaaggttgc
     6781 tgatttagct ataaaggttg agaaaattag g
//
