LOCUS       CP057449                6981 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB26-C20 plasmid pRHB26-C20_3, complete
            sequence.
ACCESSION   CP057449
VERSION     CP057449.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148251
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH09 T2-C20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 164.651488094662x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:46:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,964
            CDSs (total)                      :: 4,846
            Genes (coding)                    :: 4,715
            CDSs (with protein)               :: 4,715
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 59 of 131
            Pseudo Genes (incomplete)         :: 56 of 131
            Pseudo Genes (internal stop)      :: 44 of 131
            Pseudo Genes (multiple problems)  :: 23 of 131
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB26-C20"
                     /isolation_source="Pooled cattle faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB26-C20_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6794..6981,1..331))
                     /locus_tag="HVY34_24770"
     CDS             complement(join(6794..6981,1..331))
                     /locus_tag="HVY34_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMH43114.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFRRLGESVSEAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTASG
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(309..851)
                     /locus_tag="HVY34_24775"
                     /pseudo=""
     CDS             complement(309..851)
                     /locus_tag="HVY34_24775"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1009..1332)
                     /locus_tag="HVY34_24780"
     CDS             complement(1009..1332)
                     /locus_tag="HVY34_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMH43115.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1397..1588
                     /locus_tag="HVY34_24785"
     CDS             1397..1588
                     /locus_tag="HVY34_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMH43106.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2601..2732)
                     /locus_tag="HVY34_24790"
     CDS             complement(2601..2732)
                     /locus_tag="HVY34_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMH43107.1"
                     /translation="MRGLVFCALILILPGCQANYIRDVQGGTVAPSSSSAATGIEIR"
                     /name="colicin release lysis protein"
     gene            complement(2744..3223)
                     /locus_tag="HVY34_24795"
     CDS             complement(2744..3223)
                     /locus_tag="HVY34_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMH43108.1"
                     /translation="MIKRLLFLYVALLFLSEGAYASVTVFIAPSWERNTDSVNEIYKYI
                     DDINSNNHVIDQAYVEPIRKELGVYRDYIQKKLINFNGKGVCKLSITTGNTGVKDIEPP
                     DVVLLNSLAENSSLDCKKLYREIQSIVDNDPSLLFPEKIKLNGLLSIDMIIVMRR"
                     /name="hypothetical protein"
     gene            complement(3306..4064)
                     /locus_tag="HVY34_24800"
     CDS             complement(3306..4064)
                     /locus_tag="HVY34_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMH43109.1"
                     /translation="MSGIENSNQGPGWHDHNTPYGTIHSYDGDSHSGYGAHSGGNGGNR
                     HEGISDKTHISIPSTRNYNGYYMSMCVNNLKAHEGFKNTMYKDSEGNITVGIGHLLSTP
                     DMAASLPFTRTHTWHGHGDDMEKEVAASKKEITDAFNNFKKDSRQVPMNLHLSNDAVIG
                     LCISDVQTTEKGLRSLYSGYDGFSNSRKTALVDMGFNLGIPKLSRNFPNFNAAVNRGDW
                     ESAARESHRNLHQRGDQRNKDTADQLRDKH"
                     /name="hypothetical protein"
     gene            4318..4755
                     /locus_tag="HVY34_24805"
     CDS             4318..4755
                     /locus_tag="HVY34_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMH43110.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRR
                     EFETSELIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            4836..5255
                     /gene="exc2"
                     /locus_tag="HVY34_24810"
     CDS             4836..5255
                     /gene="exc2"
                     /locus_tag="HVY34_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMH43111.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="exc2"
     gene            5689..5898
                     /locus_tag="HVY34_24815"
     CDS             5689..5898
                     /locus_tag="HVY34_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="QMH43112.1"
                     /translation="MKSIKTFVAVTALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAHFNNRVHMTAELTK"
                     /name="DUF1471 domain-containing protein"
     gene            complement(6554..6787)
                     /gene="mbeD"
                     /locus_tag="HVY34_24820"
     CDS             complement(6554..6787)
                     /gene="mbeD"
                     /locus_tag="HVY34_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMH43113.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     Typing          complement(1678..1761)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.09e-36"
     Typing          1781..2329
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.213"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..1019)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.755"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6451..6980)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.326"
                     /coverage="99.0"
                     /evalue="6.040000000000004e-66"
ORIGIN
        1 caagatacca gagcgtcccg ccactcgcgg taaacaggaa aatgaccatc cccagtaaca
       61 tcatgcccgt attccctgcc agctttaaca cgtccttcct gtgctgcatc atcgcctctt
      121 tcaccccttc ccggtgtttt ttcagcgatt cctctgtcga ggcggtgaac agggctatag
      181 cgtctttgat tttcgtctcg tttgatgtca cagcctcgct tacagattcg ccgagcctcc
      241 tgaactcgtc gttcagcatt gtctctgtag attcggctct ctctttcagt tttttctcga
      301 actccgcgcc cgtctgtaaa agattgctca taaaatgctc cttttagtct gatattcttc
      361 ccgccgttcg ggtctgcaat actgatgctg cttcgcgtca ccctgaccac ttcaatccct
      421 gcctctgtga gcgcctgaat cacatcctga cggcctttta tctccccggc atgataaaga
      481 gcgtctatcc cgcgtgtgac gctctcagca agcgcctgtt tcgtttccgg caggttatca
      541 gggagagtca gtatccggcg gttctccggg gcgtttgggt catgcagccc gtaatggtga
      601 ttaaccagcg tctgccaggc atcaattctc ggcctgtctg cccggtcgta gtacggctgg
      661 aggcgttttc cggtctgtaa ctccatgttc ggaatgacaa aattcagctc aagccgtccc
      721 ttgtcctggt gctccaccca caggatgctg tactgatttt tttcgagacc gggcatcagt
      781 acacgctcaa aactcgccat cactttttca cgtcctcccg ctggcagctc cttctccgcg
      841 aacgagagaa caccggacgt gtatttcttc gcaaatggcg tggcatcgat gagttcccgt
      901 acttcttccg gattaccctg aagcaccgtt gccccttccc ggttacgctc ccttcccagc
      961 aggtaatcaa ccggaccact gccaccacct tttcccctgg catgaaattt aactatcatc
     1021 ccgcgtcccc tgttccctga cggccagacg cagccggcgc agctcatccc cgatggccat
     1081 cagtgcggcc actacctgaa cccggtcacc ggaagaccac tgtccactgt taaccttcct
     1141 cgctgtctga ttcaggttat tcccgatggc ggccagctga cgcagtaacg gcggtgccag
     1201 tgtcggcagt ttcccggaac gggcaaccgg ctcacccagg cagacccgcc gcatccatac
     1261 cgccagctgt ttaccctcac agcgttccag taaacgggca tgttcatcat cagtaacccg
     1321 tatcgtgagc atcctctcgc gtttcatcgg tatcattacc cccatgaaca gaaatccccc
     1381 ttacacggag gcatcagtga ctaaacagga aaaaaccgcc cttaacatgg cgcgctttat
     1441 cagaagccag acattaacgc tgctggaaaa gctcaacgaa ctggacgcgg atgaacaggc
     1501 agacatctgt gaatcgcttc acgaccacgc cgatgagctt taccgcagtt gcctcgcacg
     1561 tttcggggat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     1621 tctgtgagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtttttgcgg
     1681 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     1741 tatgcggcat cagtgcggat tgtatgaaaa gtgcaccata tgcggtgtga aatgccgcac
     1801 agatgcgtaa ggagaacatg cagataccga cgctcttccg cttcctcgct cactgactcg
     1861 ctacgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1921 ttatccacag aatcagggga taaggccgga aagaacatgt gagcaaaaag gcaaagggca
     1981 acgaaaggct ccgccgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2041 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2101 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccacttac cggatacctg
     2161 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg ttggtatctc
     2221 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2281 gaccgctgcg ccttatccgg taactatcgt ctcgagtcca acccggtaag acacgactta
     2341 acgccactgg cagcagccac tggtagagga tgtacaagaa attgctgata cgtagttctt
     2401 gaagtgaagg accgactgcg gctacactga aaggacagat ttggtggcag cggtcttgta
     2461 caccctgtta cctcgggtta gaaagcctga gaaacttcta accttcgaaa gaacccacgc
     2521 ctgagaacgt gggttttttc gtttacaggc agcagattac gcgtagaaaa aaaggatctc
     2581 aagaagatcc tttaatcttt ttacctaatt tcgattccag tagctgctga gctagaagat
     2641 ggcgcaacag tccctccttg aacatccctg atataatttg cctgacagcc gggcaatatt
     2701 aaaattagcg cacaaaaaac caaaccacgc atagaattgt attttatctc ctcataacaa
     2761 tgatcatatc aattgataac aaaccattta actttatttt ctcaggaaat aaaagtgacg
     2821 gatcgttatc aacaatactt tgtatttctc tatacagttt tttacagtct aatgatgagt
     2881 tttccgccaa tgagtttaac aaaacaacat ctggaggctc aatatcttta acacctgtat
     2941 ttcctgttgt tatcgataat ttacaaactc ccttcccatt aaagttaatt agttttttct
     3001 ggatataatc tcgatatacc cctaactcct tcctaatagg ttctacatat gcttgatcga
     3061 tgacatggtt attactattt atatcatcaa tatatttata tatctcatta actgaatctg
     3121 tattcctttc ccaacttgga gcaatgaata ctgttacaga tgcataagca ccttctgata
     3181 agaacaataa agcaacatat aagaataata accgcttgat catataatcc attcccatta
     3241 taggttgatg tatttcatct ttaatataag atgaaataca tctcatatct atatgttatg
     3301 gtctattaat gtttatcccg caactgatct gctgtatcct tgtttctctg atcgcctcgt
     3361 tgatgaagat ttcgatggct ttcacgtgct gcagactccc aatccccacg gttcacagca
     3421 gcattaaagt tcggaaagtt tctcgatagc ttaggaattc ctaagttaaa tcccatatcc
     3481 actaaggcag tttttctaga gttagaaaaa ccatcataac cagaatataa agaacgcaag
     3541 cctttctctg tagtctgaac atcggaaata cataatccaa taacagcatc atttgataaa
     3601 tgcaagttca ttggtacttg tctagagtct tttttaaagt tattaaaagc atcggttatt
     3661 tctttttttg atgctgcaac ttctttttcc atatcatcac catgaccatg ccatgtatga
     3721 gttcttgtaa aagggagaga tgccgccata tctggcgtag atagcaaatg accaatacca
     3781 acagtaatat taccttcgct atccttatac atggtgtttt tgaacccctc atgggctttt
     3841 aaattattaa cacacatgct catatagtag ccattatagt ttctggtaga tggtatacta
     3901 atatgtgtct tatcagaaat accttcatgg cgatttcccc cattaccacc agaatgagca
     3961 ccataaccgc tatggctgtc cccatcataa ctatgaattg ttccataggg agtattgtga
     4021 tcatgccacc ccggaccttg attagaattt tcaataccac tcatcttaat ttcctctttg
     4081 acatttaaaa caacgaccaa cacaactgta tatataacca ctggttttat atacagctta
     4141 aaattacgcc gctgcatttt ccctgtcaac cctgtggatt tccatttttg tgaaaacgat
     4201 caaaaaaaca gccacccaca ccacgtgcta tttccacccg atgccacaaa aaccagcaca
     4261 aacattaccg ttctccggcc tcagtatgtt ttactgaaac catgatatga gacaccaatg
     4321 ggacactgtc acttcatggc atggcacaca ctccaggacg cactgaaaat gacgggcaga
     4381 tcgcgttcac agttttaccg tgatatgcgt gcaggccttg tcagttaccg tacgagccag
     4441 gacggacgac gggagtttga aaccagtgaa ctgatccggg catacggcga attaaagcag
     4501 gatgagacac cagaaaggca cagtgcggga caggcagaaa atccacatga tcagcagaca
     4561 gaacgcattc tccgggagct gaatgagctg aaacaatgcc tgacgctgat gcttgaggat
     4621 aaacaagccc aggatacgga gcgccggcgc caggaagcag aacgggaaca gctacagaat
     4681 gagatagccc agctcaggca ggcgctggag caggaaaaga aacggggatt ctggtcccgg
     4741 ttgtttggtc gctgaacgct gtcatggact gatgataaaa tagtcttctg ataataactc
     4801 accgaaaata aatactttaa ggtagggaga ctctcatgag acgtaccgga aacaaacttt
     4861 gtcttatcgc catgataaca gcaacagtag ctctcacagc ctgtacccca aagggcagcg
     4921 tggaacaaca tacccggcat tacgtatatg cttctgatga cggttttgat cccaactttt
     4981 ccacccaaaa agccgacaca acacgaatga tggtgccttt tttccggcag ttctgggata
     5041 tgggagcaaa ggataaagcg acaggaaaat cacggagtga tgtgcagcaa cgcattcagc
     5101 agtttcacag ccaggaattt ttaaactcac tccggggcac aactcaattt gcgggtactg
     5161 attaccgcag caaagacctt accccgaaaa aatccaggct gctggctgag acgatttctg
     5221 cggtttatct cgatggctat gagggaagac agtaagtgga tttaccataa tcccttaatt
     5281 gtacgaatcc tcgaggctat tcatcctggg gttccaggcc ggaaacctgg atttttccgt
     5341 gaccagtttt ttacaggagt gtcacacaaa cgacagattg agtgacactc acagaaacag
     5401 cgatttcgac atagaattcg tcacataacc cgtcacttat tcaatgtcat gcaaagctgt
     5461 ctgtgactcg ctttaatgac ggaattttct gggattccag cttagccccc ccatcctgcc
     5521 cttaaatcag agctcacgcc tggttttgat atacccaaag tttattctgt ttttttgaac
     5581 aacatcagca aattgtttcg attttaatca gcctatcgtc gtcctatcat ttatcacatc
     5641 atggcaacaa cgccatatac agaacaaaat gacttaagga atatgaccat gaaatccatc
     5701 aaaactttcg ttgcagttac cgctctatcc atgatgtctt tcggtgcatt tgctcagagc
     5761 gtcagcgcta ccgcctccac tctggatcgt gctgaagcaa aaattgccgc acaggccgct
     5821 gaacaaggtg cgtcatacaa aatcaccagc gctcacttca ataaccgtgt ccacatgacg
     5881 gctgaactga ccaaataaga tggtcgcaga gggagctgcc gaacaggtcg ccgggaggcg
     5941 gcctgtttcg tccctaagca ggaaaacctt caggcccctt cgtttttttt gcatctgtca
     6001 tttaacataa aggcatgatt gtacgcaccg ctgaaacgcg ttcggtgcga tcacggcagc
     6061 agacaggtaa aaatggcaac aaaccacccg aaaaactgcc gcgatcgcac ccggtaaatt
     6121 ttaaccacat gcatagctat gcagccatgt gaatcacgca ggaattgcca gccagagact
     6181 gctgaaactg atttttttca tactttagga agcgaagaag acgggactgg agcggaaaaa
     6241 cagaagttca ggggaatgag cgcgatctgg caataaaggc aaaaacacag caacaaagac
     6301 acaccagaat cgcgcccgta tgcgttttaa cgcgttcctg tgttttcagc aaggtctgac
     6361 tgaagcgcgt tcccggtgcc ccgaaccagg cattatccca aaaaatgcac cggaaacgcg
     6421 ctgacaacat tttagcctgt ctctggttac catcccagcg aagggccatc cagtgtccgt
     6481 tcctgtattt ccggctggcg ctcacgctcc agagataact catgttcgcg ttcctgtatc
     6541 agccgttcat cccttatctc ctgttctcgc tgtataactg gctcaagcgt tctgtccgct
     6601 cgctcaagtg ctgcacctgc tgactcaact tcatgacccg ctcgttcagc atcgcgttgt
     6661 cccgttgcgt aagcgaaaac attttctgca attccacgaa ggcgctctcc cattcgttca
     6721 gccgctgcat atagtcctgt tgcagttgct ctaatgcgtt cagcaaatgt ctttccagct
     6781 ccgtcactct gtgtcactcc gtcagttgta cccagtcctt cccctgatag ggaatcacct
     6841 ctgctgattt cccgtacgga aggaccagaa atttcctgtt cccgtcctgt acgaactcca
     6901 cgccccatgt cttcgcgttc agtttctgca atgtctcttc ctgcttcctg atatcttcca
     6961 ggttcgcctg tatcatcccg c
//
