LOCUS       CP057425                6888 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB27-C09 plasmid pRHB27-C09_3, complete
            sequence.
ACCESSION   CP057425
VERSION     CP057425.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148259
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH04 T2-C09
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 191.788268809151x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:25:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,933
            CDSs (total)                      :: 4,814
            Genes (coding)                    :: 4,634
            CDSs (with protein)               :: 4,634
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 64 of 180
            Pseudo Genes (incomplete)         :: 110 of 180
            Pseudo Genes (internal stop)      :: 29 of 180
            Pseudo Genes (multiple problems)  :: 22 of 180
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB27-C09"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB27-C09_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            238..675
                     /locus_tag="HVY42_24575"
     CDS             238..675
                     /locus_tag="HVY42_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMH11295.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="entry exclusion protein 1"
     gene            756..1175
                     /gene="exc2"
                     /locus_tag="HVY42_24580"
     CDS             756..1175
                     /gene="exc2"
                     /locus_tag="HVY42_24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMH11296.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(1738..1971)
                     /gene="mbeD"
                     /locus_tag="HVY42_24585"
     CDS             complement(1738..1971)
                     /gene="mbeD"
                     /locus_tag="HVY42_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMH11297.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(1978..2496)
                     /locus_tag="HVY42_24590"
     CDS             complement(1978..2496)
                     /locus_tag="HVY42_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMH11298.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            complement(2474..3232)
                     /locus_tag="HVY42_24595"
                     /pseudo=""
     CDS             complement(2474..3232)
                     /locus_tag="HVY42_24595"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3174..3497)
                     /locus_tag="HVY42_24600"
     CDS             complement(3174..3497)
                     /locus_tag="HVY42_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMH11303.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3562..3753
                     /locus_tag="HVY42_24605"
     CDS             3562..3753
                     /locus_tag="HVY42_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMH11299.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4778..4915)
                     /locus_tag="HVY42_24610"
     CDS             complement(4778..4915)
                     /locus_tag="HVY42_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMH11300.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            4963..5304
                     /locus_tag="HVY42_24615"
     CDS             4963..5304
                     /locus_tag="HVY42_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="QMH11301.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin transporter"
     gene            complement(5301..6866)
                     /locus_tag="HVY42_24620"
     CDS             complement(5301..6866)
                     /locus_tag="HVY42_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /protein_id="QMH11302.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-10"
     Typing          complement(3839..3926)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          3937..4495
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.428"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1635..3184)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.031"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaacaaata gttaaaagca atatactgta tatataatca ctggttttat atacagttta
       61 aaattacgcc gctgcatttt ccctgtcaac cctgtggatt ttcatttttg tgaaaacgat
      121 caaaaaaaca gccacccaca ccacgtgcta tttccacccg atgccacaaa aaccagcaca
      181 aacattaccg ttctcagacc tcattatgtt ttactgaaac catgagatga gacacctatg
      241 ggacactgta actttatggc atggcacaca ctccgggacg cactgaaaat gactggaaga
      301 tcgcgttcac agttttaccg tgatatgcgc tcaggtcttg tcagttaccg taccagccag
      361 gacggacgac gggagtttga aaccagtgaa ctgatccggg catacggcga attaaagcgg
      421 aatgagacac cagaaaggca cactcaagga cactcagaaa aacctcatga tcagcagaca
      481 gaacgcattc tccgggaact gaatgagctg aaacaatgcc tgacgctgat gcttgaggat
      541 aaacaagccc aggatacgga gcgccggcgc caggaagcag aacggaaaca gctacagaat
      601 gagatagccc agctcaggca ggctctggag caggaaaaga aacggggatt ctggtccagg
      661 ctgttcggtc actgaacgcg ctcagaaact gatgataaga taatcttcgg ataataactc
      721 accgagaata aatactttaa ggtagggaga cactcatgag acgtaccgga aacaaacttt
      781 gtcttatcgc catgataaca gcaacagtag ctctcacagc ctgtacccca aagggcagcg
      841 tggaacaaca tacccggcat tacgtatatg cttctgatga cggttttgat cccaactttt
      901 ccacccaaaa agccgacaca acacgaatga tggtgccttt ttttcggcag ttctgggata
      961 tgggagctaa agataaagcg acaggaaaat cacggagtga tgtgcaacaa cgcattcaac
     1021 agtttcacag ccaggaattt ttaaactcac tccggggcac aactcaattt gcgggtactg
     1081 attaccgcag caaagacctt accccgaaaa aatccaggct gctggctgac acgatttctg
     1141 cggtttatct cgatggctac gagggcagac agtaagtgga tttaccataa tcccttaatt
     1201 gtacgcaccg ccgaaacgcg ttcggtgcga tcacggcagc agacaggtaa aaatggcaac
     1261 aaaccacccg gaaaactgcc gcgatcgcac ccggtaaatt ttaaccacat gcatacctat
     1321 gcagccaggt gaatcacgca ggaattgcca gtcagagacc gctgaaacgg atttttttca
     1381 tactttcgga agcgaagaag acggggattt gagcaggaaa acagaagttc aggggaatga
     1441 gcgcgatctg gcaatagagg caaaaacacg actatgaaag acacaccaga atcgcgcccg
     1501 tatgcgtttt aacgcgttcc tgtgttttca gcaaggttta tgactgaagc gcgttcccgg
     1561 tgccccgaac caaccattat cccaaaaaat gcaccggaaa cgcgctgaca acattttagc
     1621 ctgtctctga ttaccatccc agcgaagggc catccagtgt ccgttcctgt atttccagct
     1681 ggcgctcacg ctccagggat aactcatgtt cgcgttcctg tatcagccgt tcgtccctta
     1741 tctcctgttc tcgctgtata actggctcaa gcgttctgtc cgctcgctca agtgctgcac
     1801 ctgctgactc aacttcatga cccgctcgtt cagcatcgcg ttgtcccgtt gcgtaagcga
     1861 aaacattttc tgcaattcca cgaaggcgct ctcccattcg ttcagccgct gcatatagtc
     1921 ctgttgcagt tgctctaatg cgttcagcaa atgtctttcc agctccgtca ctctgtgtca
     1981 ctccatcagt tgtacccagt ccttcccctg atggggaatc acctctgctg atttcccgta
     2041 cggaaggacc agaaatttcc tgttcccgtc ctgtacgaac tccacgcccc atgtcttcgc
     2101 gttcagtttc tgcaatgtct cttcctgctt cctgatatct tccaggttcg cctgtatcat
     2161 ccctccaaga taccagagcg tcccgcaact cacggtaaac aggaaaatga ccatccccag
     2221 taacatcatg cccgtattcc ctgccagctt taacacgtcc ttcctgtgct gcatcatcgc
     2281 ctctttcacc ccttcccggt gctttttcag tgattcctct gtcgaagctg tgaacagggc
     2341 tatagcgtct ttgattttcg tctcgtttga tgtcacagcc ttgcttacag atttttcgag
     2401 cttgctgaac tcgtcgttca gcattgtctc tgtagattcg gctctctctt tcagcttttt
     2461 ctcgaactcc gcgcccgtct gtaaaagatt gctcatagaa tgctcctttc agtctgatat
     2521 tcttcccgcc gttcgggtct gcaatgctga tactgcttcg cgtcaccctg accacttcaa
     2581 tccctgcttc cgtgagcgcc tgaatcacat cctgacggcc ttttatctct cctgcatggt
     2641 aaagagcatc tatcccgcgt gtgacgccct cagcaaacgc ctgtttcgtt tccggcaggt
     2701 tatcagggag agtcagcgtc cggcggttct ccggggcgtt cgggtcatgc agcccgtaat
     2761 ggtgattcac cagcgtctgc caggcatcaa ttctcggcct gtctgcccgg tcgtagtacg
     2821 gctgtaggcg ttttccggtc tccagctcca tgttcggaat gacaaaattc agctcaagcc
     2881 gtcccttgtc ctggtgctcc acccacagga tgctgtactg atttttttcg agaccgggca
     2941 tcagtacacg ctcaaagctc gccatcactt tttcacgtcc tcccggcggc agctccttct
     3001 ccgcgaacga cagaacaccg gacgtgtatt tcttcgcaaa tggcgtggca tcgatgagtt
     3061 cccggacttc ttccggatta ccctgaagca ccgttgcccc ttcccggtta cgctcccttc
     3121 ccagcaggta atcaaccgga ccactgccac caccttttcc cctggcatga aacttaacta
     3181 tcatcccgcg ccccctgttc cctgacggcc agacgcaacc ggcgcagctc atccccgatg
     3241 gccatcagtg cggccaccac ctgaacccgg tcgccggaag accactgtcc gctgttcacc
     3301 ttacgggctg tctggttcag gttattcccg atggctgcca gctgacgcag taacggcggt
     3361 gccagtgtcg gcagcctccc ggaacgggca accggctcac ccaggcagac ccgccgcatc
     3421 cataccgcca gttgtttacc ctcacagcgt tccagtaacc gggcatgttc atcatcagta
     3481 acccgtatcg tgagcatcct ctcgcgtttc atcggtatca ttacccccat gaacagaaat
     3541 cccccttaca cggaggcatc agtgactaaa caggaaaaaa ccgcccttaa catggcccgc
     3601 tttatcagaa gccagacatt aacgctgctg gagaagctca acgaactgga cgcggatgaa
     3661 caggcagata tctgtgaatc gcttcacgac cacgccgatg agctttaccg cagctgcctc
     3721 gcgcgcttcg gggatgacag tgaaaacctc tgacatatgc agctcccgga gacggtcaca
     3781 gcttgcctgt gagcggatgc cgggagcaga caagcccgcc agggcgcgtc agggtgtttt
     3841 agcgggtttc ggggcgcagc cctgaaccag tcacgtagcg atagcggagt gtatactggc
     3901 ttaactatgc agcatcagtg cggattgtat gaaaagtgca ccatgaccag tgtgaaatac
     3961 cgcacagatg cgtaaggaga aaatgcacgt tccgccgctt ttccgcttcc tcgctcactg
     4021 actcgctacg ctcggtcgtt cggctgcggc gagcggtact gactcactca aaaacgataa
     4081 tacagttatc cacagaatcc ggggataaga ccggaaagaa catgtgcgca aaaaggcaaa
     4141 gcccaacaaa aggccacgcc gatggcgttt ttccataggc tccgcccccc tgacgagcat
     4201 cacaaaaacc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4321 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgttgg
     4381 tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4441 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4501 gacttagcgc cactggcagc agccgttggt aacttggtta gcagagcgat gatggcgcaa
     4561 atggtgcaac agagttcttg aagtagtggc ccgactacgg ctacactgga aggacagtat
     4621 ttggtatctg cgctctgctg aagccagtta cctcggttaa gcagttcccc aactgactta
     4681 accttcgatc aaaccgcctc cccaggcggt tttttcgttt acaggcagca gattacgcgc
     4741 agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaacagag atccccgtca
     4801 atttagaaga agatgatgga gccacagtcc ctccctgaac atcacgtata tagttagcct
     4861 gacatccaac aaggaggttt atagcgaata ttcccacaaa aaatcttttc ctcataactc
     4921 gatccttata aaatgaaaag aatatataac gaggtttaat ttatgagttt aagatactac
     4981 ataaaaaata ttttgtttgg cctatactgc gcacttatat atatatacct tataacaaaa
     5041 aacaacgaag ggtattattt cctagcgtca gataagatgc tatacgcaat agtgataagc
     5101 actattctat gcccatattc aaaatatgct attgaacaca tattttttaa gttcataaag
     5161 aaagattttt tcagaaaaag aaaaaaccta aataatgccc ccgtggcaaa attaaaccta
     5221 tttatgctat acaatctact ttgtttggtc ctagcaatcc catttggatt gctaggactg
     5281 tttatatcaa taaagaataa ttaaataccc aacacctcat ttatagtatt aagtttattc
     5341 ttatcaataa aggcacatag aatgcctgta acaatagcaa tcccccagat acctaatgta
     5401 gttccagcaa gcacactaaa aagtaaaaca acaacataac taactccagc atctacagct
     5461 ttcttctcta atgtaagaaa tagcggcttc cagtcacctg tatccttaat ttttaggata
     5521 tcagatacca catcatatcc aaaagaaaca tgtcccgtaa tcttcaagta cttggcaaac
     5581 tgatctaaat gcttagccca gtcttcatac ttaaccgcct ccagtgcatt gaaaattgca
     5641 tcacggtctg ctttgctgaa tttcttattt aatacatcct tgtatttctc aaaagcagct
     5701 agagcttcat tcacattgct gattttctta cctttagatt tatcagcaag ttcctgtgcc
     5761 atttttgaat atttttcacc atatttttca gatagcgttt gataaaagct aactgttgca
     5821 tccacagcat ccttaatctg cgagttcagg agattagtct gtgctgtttt caaattatct
     5881 tcagcatcat gaatacgtga aactgctgta cttcgcttat tattagcctg agaaatagcc
     5941 tcttggattc catttatctc agagctaatc tggttaagac gcgtttctga tgctgttacc
     6001 tgcttttgtt tttcctccat ctcatcacca gctctcgccc gtagtctggt ggcctcaaaa
     6061 aaaggacgac tctgaagcgg atcattcgcc ctaggagata aaagtttaac cctttcatcc
     6121 agttctttat atttagcaga tgcctgatcc aactcatttc gttttccgga cagcgtattc
     6181 gtttctgcat cacgggcatg aatactggag cttaaacggg tattgagcgt attaatctct
     6241 tcatccactt tcgccagctc agattgtgca gcagcaagat ttttttgtgc cacctccaca
     6301 gcccgggcct cttcactcaa tgctgccagg cgtttctctt catcctcagc cagtttcaat
     6361 tggcgttctg tttcagcctg ctccttctct atctctttac gtcgttgttc tgcttcctga
     6421 aaagcctttt ccgcagcttc cgcttcttta cgggcttttt cttctgcttt tgcaaggcgc
     6481 aaccgctctg cttctgcctg catcgctgca ttattggcat gagccaggtc aataacctcc
     6541 ggatgagtgg aattatggcg aagcgcctca ttcacaatat ccttcagatg ttgagtcagc
     6601 gcatcccggt ttgcttttgc tttagcctgt gcttctgcgg cagcttttgc tcgggcagcc
     6661 tgttctgcct gcgttttctt caattgagca gtagaccatt tagctgtggc atgaatggct
     6721 gcagaacttt cacttttgct acctccagtt ccaccaccac cgccagagcc actcccgtct
     6781 ggattaccat tcaaaagagt aatgattacc tgccccttat catcataagg aacaccatct
     6841 ttatagtacg ctacagctgt ttccattata aaatcctctt tgacatta
//
