LOCUS       CP057414                6647 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB27-C20 plasmid pRHB27-C20_7, complete
            sequence.
ACCESSION   CP057414
VERSION     CP057414.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148263
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH04 T2-C20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 146.358368757903x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:24:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,633
            CDSs (total)                      :: 5,500
            Genes (coding)                    :: 5,127
            CDSs (with protein)               :: 5,127
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 373
            CDSs (without protein)            :: 373
            Pseudo Genes (ambiguous residues) :: 0 of 373
            Pseudo Genes (frameshifted)       :: 136 of 373
            Pseudo Genes (incomplete)         :: 211 of 373
            Pseudo Genes (internal stop)      :: 61 of 373
            Pseudo Genes (multiple problems)  :: 34 of 373
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB27-C20"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB27-C20_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6521..6647,1..107))
                     /gene="mbeD"
                     /locus_tag="HVY46_28070"
     CDS             complement(join(6521..6647,1..107))
                     /gene="mbeD"
                     /locus_tag="HVY46_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMG97775.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(114..632)
                     /locus_tag="HVY46_28075"
     CDS             complement(114..632)
                     /locus_tag="HVY46_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="QMG97776.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mobilization protein MbeB"
     gene            complement(610..1152)
                     /locus_tag="HVY46_28080"
                     /pseudo=""
     CDS             complement(610..1152)
                     /locus_tag="HVY46_28080"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1310..1633)
                     /locus_tag="HVY46_28085"
     CDS             complement(1310..1633)
                     /locus_tag="HVY46_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMG97777.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1697..1888
                     /locus_tag="HVY46_28090"
     CDS             1697..1888
                     /locus_tag="HVY46_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMG97778.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2915..3052)
                     /locus_tag="HVY46_28095"
     CDS             complement(2915..3052)
                     /locus_tag="HVY46_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMG97779.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            3100..3441
                     /locus_tag="HVY46_28100"
     CDS             3100..3441
                     /locus_tag="HVY46_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="QMG97780.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(3438..5006)
                     /gene="cea"
                     /locus_tag="HVY46_28105"
     CDS             complement(3438..5006)
                     /gene="cea"
                     /locus_tag="HVY46_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="QMG97781.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            5266..5703
                     /locus_tag="HVY46_28110"
     CDS             5266..5703
                     /locus_tag="HVY46_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMG97782.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            5784..6203
                     /gene="exc2"
                     /locus_tag="HVY46_28115"
     CDS             5784..6203
                     /gene="exc2"
                     /locus_tag="HVY46_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMG97783.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     Typing          complement(1974..2061)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2072..2630
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..1320)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.773"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcttctgca attccacgaa ggcgctctcc cattcgctca gccgctgcat atagtcctgt
       61 tgcagctgct ctaaggcgtt cagcaaatgt gtttccagct ctgtcactct gtgtcactcc
      121 ttcagatgta cccactcttt cccctgaaag ggaatcacct ccgctgattt cccgtacgga
      181 aggacaagga atttcctgtt cccgtcctgc acaaactcca cgccccatgt cttcgcgttc
      241 agtttctgca atgtctcttc ctgcttcctg atttcttcca ggttcgcctg tatcctccct
      301 ccaagatacc agagcgtccc gccactcgcg gtaaacagga gaaagactat ccccagtaac
      361 atcatgcccg tattccctgc cagctttaac acgtccctcc tgtgctgcat catcgcctct
      421 ttcacccctt cccggtgttt ttccagcgat tcctctgtcg aggctgtgaa cagggctata
      481 gcgtctctga ttttcgtctc gtttgatgtc acagcctcgc ttacagattc gccgagcctc
      541 ctgaactcgt tgttcagcat tttctctgta gattcggctc tctctttcag ctttttctcg
      601 aactccgcgc ccgtctgcaa aagattgctc ataaaatgct cctttcagcc tgatattctt
      661 cccgccgttc ggatctgcaa tgctgatact gcttcgcgtc accctgacca cttccagccc
      721 cgcctcagtg agcgcctgaa tcacatcctg acggcctttt atctctccgg catggtaaag
      781 tgcatctata cctcgcgtga cgccctcagc aagcgcctgt ttcgtttcag gcaggttatc
      841 agggagtgtc agcgtcctgc ggttctccgg ggcgttcggg tcatgcagcc cgtaatggtg
      901 atttaccagc gtctgccagg catcaattct aggcctgtct gcgcggtcgt agtacggctg
      961 gaggcgtttt ccggtctgta gctccatgtt cggaatgaca aaattcagct caagccgtcc
     1021 cttgtcctgg tgctccaccc acaggatgct gtactgattt ttttcgagac cgggcatcag
     1081 tacacgctca aagctcgcca tcaccttttc acgtcctccc ggcggcagct ccttctccgc
     1141 gaacgacaga acacctgacg tgtatttctt cgcaaatggc gtggcatcga tgagttcccg
     1201 gacttcttcc ggattaccct gaagcaccgt tgcgccttcg cggttacgct ccctccccag
     1261 caggtaatca accggaccac tgccaccacc ttttcccctg gcatgaaatt taactatcat
     1321 cccgcgcccc ctgttccctg acagccagac gcagccggcg cagctcatcc ccgatggcca
     1381 tcagtgcggc caccacctga acccggtcac cggaagacca ctgcccgctg ttcaccttac
     1441 gggctgtctg attcaggtta tttccgatgg cggccagctg acgcagtaac ggcggtgcca
     1501 gtgtcggcag ttttccggaa cgggcaaccg gctcccccag gcagacccgc cgcatccata
     1561 ccgccagttg tttaccctca cagcgttcaa gtaaccgggc atgttcatca tcagtaaccc
     1621 gtattgtgag catcctctcg cgtttcatcg gtatcattac cccatgaaca gaaatccccc
     1681 ttacacggag gcatcagtga ctaaacagga aaaaaccgcc cttaacatgg cccgctttat
     1741 cagaagccag acattaacgc tgctggagaa gctcaacgaa ctggacgcag atgaacaggc
     1801 cgatatttgt gaatcgcttc acgaccacgc cgatgagctt taccgcagct gcctcgcacg
     1861 tttcggggat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     1921 tctgtgagcg gatgccggga gctgacaagc ccgtcagggc gcgtcagcag gttttagcgg
     1981 gtgtcggggc gcagccctga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     2041 catgcggcat cagtgcggat tgtatgaaaa gtacgccatg ccgggtgtga aatgccgcac
     2101 agatgcgtaa ggagaaaatg cacgtccagg cgcttttccg cttcctcgct cactgactcg
     2161 ctacgctcgg tcgttcgact gcggcgagcg gtactgactc acacaaaaac ggtaacacag
     2221 ttatccacag aatcagggga taaggccgga aagaacatgt gagcaaaaga ccaggaacag
     2281 gaagaaggcc acgtagcagg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2341 aaatagacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2401 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2461 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gttggtatct
     2521 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2581 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa ggcacgcctt
     2641 aacgccactg gcagcagcca ctggtaaccg gattagcaga gcgatgatgg cacaaacggt
     2701 gctacagagt tcttgaagta gtggcccgac tacggctaca ctagaaggac agtatttggt
     2761 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2821 aaacaaacca ccgttggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2881 aaaaaaggat ctcaagaaga tcctttaatc ttttctactg aaccgcgatc cccgtcagtt
     2941 tagaagagga ggatggtgcg atggtccctc cctgaacatc acgtatatag ttagcctgac
     3001 atccaacaag gaggtttatc gcgaatattc ccacaaaaaa tcttttcctc ataactcgat
     3061 ccttataaaa tgaaaagaat atatggcgag gtttaattta tgagcttaag atactacata
     3121 aaaaatattt tatttggcct gtactgcaca cttatatata tataccttat aacaaaaaac
     3181 agcgaagggt attatttcct tgtgtcagat aagatgctat atgcaatagt gataagcact
     3241 attctatgtc catattcaaa atatgctatt gaatacatag cttttaactt cataaagaaa
     3301 gattttttcg aaagaagaaa aaacctaaat aacgcccccg tagcaaaatt aaacctattt
     3361 atgctatata atctactttg tttggtccta gcaatcccat ttggattgct aggacttttt
     3421 atatcaataa agaataatta aatccctaac acctcattta tagtattaag tttattctta
     3481 tcaatatagg agcatagaat tcctgtaaca atagcaatac cccaaatacc taatgtagtt
     3541 ccagcaagca agctaaaaag taaagcaaca acataactca cccctgcatc tgcagctttc
     3601 ttctctaatg taagaaatag tggcttccag tcacctgtat ccttaatttt taggatatca
     3661 gataccacat catatccaaa agaaacatgc cccgtaatct tcaagtactt ggcaaactga
     3721 tctaaatgtt tagcccagtc atcatacttc accgatgcca acgcattaaa aatagcatca
     3781 cgatcggctt tgctgaattt cttatttaaa acatccttgt atttttcaaa agcagcgaga
     3841 gcttcattca cattgccgat tttcttacct ttagacttat cagcaagttc ctgtgccatt
     3901 ttcgaatatt tttcaccata tttttcagtc agcgtttgat aaaagctaac tgttgcatca
     3961 acagcatcct taatctgtga attaaggaga ttattctgtg cttttttcaa attttcttca
     4021 gcttcatgaa cacgagcgat accggcatta cgattattac tgacctgaga aatagccttc
     4081 tggatctgag ttatatcagc atttatccgg ttaatacgtg tttctgatgc tgttacctgt
     4141 ttttgttttt cttctctaat cttaccggcc ccaacccgtc gtctggttgc ttcaaaaaaa
     4201 ggacggttct gaagcggatc attggctctt ggtgatagtt ttttgaccag ctcatccagt
     4261 tctttatatt tagcggatgc ctgagccagt tcatttcgtt ttccagcgag cgttttcatt
     4321 tctgcatcac gggcatggat actggagctt aaacgagaat tgagagtctt aatctctcca
     4381 tccattttca ccacttcaga ttgtgcagca gaaagttttt tttgggcgat ctcaacagct
     4441 ttagcttctt cactcaatgc agccagtcgt ttctcttcag cttcagccag tttcaactgg
     4501 cgttctgttt cagccttctc ccgttcaatc tctttacgtc gttgttctgc ttcctgaaaa
     4561 gccttttctg ctgcttccgc ttctttacgg gctttttctt ctgctttcgc aaggcgcaaa
     4621 cgctctgctt ccgcctgcat agctgcatta ttagcatgag caagctctgt tgctgaaggc
     4681 gtacgtgagg cattgtgacg aagagcctca ttcacgatat ccttcaggcg ctgagtcagc
     4741 gcatccctgt ttgcctttgc tttcgcctgt gcttccgctg cagcttttgc ccgggcagcc
     4801 tgctctgcct gtgttttctt taattgagca gtagaccatt tagcagttgc atgaatagct
     4861 gcagaacttt cacttttact gcctcctttt ccacctccgc cgccagagcc actcccgtca
     4921 ggagtaccat tcaaaagagt aataattacc tgtcccttat catcataagg aacaccatct
     4981 ttatagtacg ctaccgcggt ttccattata aaatcctctt tgacttttaa aacaataagt
     5041 taaaaataaa tactgtacat ataaccactg gttttatata cagcataaaa gctacgccgc
     5101 tgcactttcc ctgtcaagac tgtggacttc catttttgtg aaaacgatca aaaaaacagt
     5161 ctttcacacc acgcgctatt ctcgcccgat gccacaaaaa ccagcacaaa cattaccgtt
     5221 ctcagacctc attatgtttt actgaaacta tgagatgaga catctatggg acactgtcac
     5281 tttatggcat ggcacacact ccgggacgca ctaaaaatga caggcagatc gcgttcacag
     5341 ttttaccgtg atatgcgcgc aggccttgtc agttaccgta ccggcaggga cggacgacgg
     5401 gagtttgaaa ccagtgaact gatccgggca tacggcgaat taaagcagaa tgagacacca
     5461 gaaaggcaca gtgagggaca tgcagaaaat ccacatgatc agcagacaga acgcattctc
     5521 cgggaactga atgagctgaa acaatgcctg acgctgatgc ttgaggataa acaggcacag
     5581 gatatggatc gcagacgcca ggaagcagaa cgggaacagc tacaaaatga gatagcccag
     5641 ctcaggcagg cactggaact ggaaaagaaa cggggattct ggtccaggtt gttcggtcgc
     5701 tgaacgctgt cagagactga tgataaaata gtcttcggat aataactcac cgagaataaa
     5761 tactttaagg tagggagaca ctcatgagac gtaccggaaa caaactttgt cttatcgcca
     5821 tgataacagc aacagtagct ctcacagcct gtaccccaaa gggcagcgtg gaacaacata
     5881 cccggcatta cgtatatgct tctgatgacg gttttgatcc caacttttcc acccaaaaag
     5941 ccgacacaac acgaatgatg gtgccttttt ttcggcagtt ctgggatatg ggagctaaag
     6001 ataaagcgac aggaaaatca cggagtgatg tgcaacaacg cattcagcag tttcacagcc
     6061 aggaattttt aaactcactc cggggcacaa ctcaatttgc gggtactgat taccgcagca
     6121 aagaccttac cccgaaaaaa tccaggctgc tggctgacac gatttctgcg gtttatctcg
     6181 atggctacga gggcagacag taagtggatt taccataatc ccttaattgt acgcaccgct
     6241 aaaacgcgtt cagcgcgatc acggcagcag acaggtaaaa atggcaacaa accacccgaa
     6301 aaactgccgc gatcgcgcct gataaatttt aaccgtatga atacctatgc aaccagaggg
     6361 tacaggccac attaccccca cttaatccac tgaagctgcc atttttcatg gtttcaccat
     6421 cccagcgaag ggccatccag cgtgcgttcc tgtatttccg gctgacgctc ccgttccagg
     6481 gataacacat gttcgcgctc ctgtatcagc cgttcctctc ttatctccag ttctcgctgt
     6541 ataactggct caagcgttct gtctgctcgc tcaagtgctg cacctgctga ctcaactgca
     6601 tgacccgctc gttcagcatc gcgttgtccc gttgcgtaag cgaaaac
//
