LOCUS       CP057413                7971 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB27-C20 plasmid pRHB27-C20_6, complete
            sequence.
ACCESSION   CP057413
VERSION     CP057413.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148263
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH04 T2-C20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 146.358368757903x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:24:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,633
            CDSs (total)                      :: 5,500
            Genes (coding)                    :: 5,127
            CDSs (with protein)               :: 5,127
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 373
            CDSs (without protein)            :: 373
            Pseudo Genes (ambiguous residues) :: 0 of 373
            Pseudo Genes (frameshifted)       :: 136 of 373
            Pseudo Genes (incomplete)         :: 211 of 373
            Pseudo Genes (internal stop)      :: 61 of 373
            Pseudo Genes (multiple problems)  :: 34 of 373
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB27-C20"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB27-C20_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(179..673)
                     /locus_tag="HVY46_28030"
     CDS             complement(179..673)
                     /locus_tag="HVY46_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97767.1"
                     /translation="MAGTVWNDVNTGREVFSMTHLHPQLHKVEVSGVLVEIEISFGFHV
                     FTDQKQVGKVMSFRGETRHFCQERYEGSKTIVQRILSAIENGEYITAFISKGQGQRYYH
                     LNHHDDFILMEIRKPQGKDNCLRIHVVTAYTMDEWGGVNKGRNLKFRYVLEQRLQGKKI
                     V"
                     /name="hypothetical protein"
     gene            complement(677..1108)
                     /locus_tag="HVY46_28035"
     CDS             complement(677..1108)
                     /locus_tag="HVY46_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97774.1"
                     /translation="METWDRNDRPRNDGFITVPRYLPLLGVLMDELSKGSPLSSTYLAL
                     WFRGSDEGLIEIRDKTVLALESGFASARGVTTWTGRMRKLKELGFISCREGSSGEFHNV
                     LIVHPLVAVKKLLDEGKITKGKTYNTFAERVIEVKSSWE"
                     /name="hypothetical protein"
     gene            complement(2253..2402)
                     /locus_tag="HVY46_28040"
     CDS             complement(2253..2402)
                     /locus_tag="HVY46_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97768.1"
                     /translation="MPSEYDEQRDATDALLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            2996..3109
                     /locus_tag="HVY46_28045"
     CDS             2996..3109
                     /locus_tag="HVY46_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMG97769.1"
                     /translation="MHEPPVQSDRCAFSGNYQLESNPERHDKTPMAAATGN"
                     /name="replication initiation protein"
     gene            complement(3344..3781)
                     /locus_tag="HVY46_28050"
     CDS             complement(3344..3781)
                     /locus_tag="HVY46_28050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97770.1"
                     /translation="MPKRGKSIRSSSRNEETKPSKSTAKTTDRFISISFKHFHNVDDVG
                     QSINTWKDDGSLPDLIEKLVYLTANGITKVISDGVFTNYTRFPESAVNDFRCPDEISSD
                     EDWGVIKNIGGQKRRVAGFLRDNVFYIVFFDRDHRFWKSQK"
                     /name="hypothetical protein"
     gene            complement(3786..5084)
                     /locus_tag="HVY46_28055"
     CDS             complement(3786..5084)
                     /locus_tag="HVY46_28055"
                     /inference="COORDINATES: protein motif:HMM:NF024700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="QMG97771.1"
                     /translation="MMVNFSLSNAFSFMEAQNMSMLAVSSSKDDINLANVVSVRDERIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESGITKNVVPFLLDEAGPTKPSEME
                     MTFIADGIKYRYGLSLFQGKIKEEWLYYTPKTRETILFERDGQLIEINKAAFSESNLFI
                     KDGVIQKTREDVPFVSVVAGFNGEHSKRVVNWFSNVVFLSGSNEGMFTGITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPEFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTANGEISVPIDFESEGTKKIVYLLGPIYNAMINNNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGFSQVIAAVQDVNLMNTDCFRRDQIWFVDKDVKSGSSHLYSLVEYKEKQRS
                     LKPSYGNDYLSGAFDAIPLFDNLNEFEKLMPEG"
                     /name="ATP-binding protein"
     gene            6843..7037
                     /locus_tag="HVY46_28060"
     CDS             6843..7037
                     /locus_tag="HVY46_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMG97772.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7648..7809
                     /locus_tag="HVY46_28065"
     CDS             7648..7809
                     /locus_tag="HVY46_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMG97773.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSLE
                     VMRLLRLN"
                     /name="replication initiation protein"
     Typing          complement(2431..2517)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.591"
                     /coverage="99.0"
                     /evalue="2.4800000000000002e-33"
     Typing          complement(6328..6597)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="93.0"
                     /evalue="9.77e-93"
     Typing          complement(6709..6807)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          complement(7115..7287)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.857"
                     /coverage="100.0"
                     /evalue="4.41e-59"
     Typing          2528..3065
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.993"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          7212..7762
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.514"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 cccgtgaagt tgacaagagg catgggtaag tagacaatat acctgttagc acaaaccgga
       61 accttgaaag aggggtgctg atgccggggg aaaaccctcc tagccagtga gcaggataac
      121 taaaggccag accgaaattt ttgcagtaaa acaaaggcgc ctagggcgct ttttgttttt
      181 atacgatttt cttcccctgc aacctctgct ccagaacata cctgaacttc aaattgcgtc
      241 ctttgttaac ccccccccat tcatccatcg tatacgctgt aacaacgtgt attcgaagac
      301 aattatcttt accttgaggc tttcttatct ccattagaat gaagtcatca tgatggttaa
      361 ggtggtaata tcgctgccct tgtcccttgg aaataaaggc cgttatgtac tctccatttt
      421 ctatcgctga cagtatcctc tgcacgatag tcttacttcc ttcgtatctt tcttggcaga
      481 aatgtctagt ttcccctctg aaactcatta cctttccgac ctgtttttgg tcagtaaata
      541 catgaaaccc gaagctaatc tcaatctcaa ccagtacccc agaaacctca accttatgca
      601 gctgagggtg caggtgtgtc atcgagaaaa cttcacgccc tgtattaaca tcattccata
      661 ccgtacctgc catttcttac tcccatgagg acttcacctc gattacccgt tcagcaaaag
      721 tgttataggt tttccctttc gttattttcc cttcatccag tagtttcttt accgcaacca
      781 gcggatgaac aatcaacacg ttgtggaact caccggagct accttcccgg catgatataa
      841 atcccagctc ttttaatttc cgcattctcc ccgtccatgt tgttaccccc cttgcactgg
      901 caaagcctga ttctaaagcc agcacagtct tatcccttat ctctatcagg ccttcgtctg
      961 aacccctgaa ccacagcgct aggtaggtac ttgaaagtgg gcttcccttt gataactcat
     1021 ccataagaac acctagtaat ggaagataac gaggtactgt tatgaagcca tcgtttcgag
     1081 gtcggtcatt acgatcccat gtttcaattt cctccccttc ccataatgta tttatcagtt
     1141 cgattcttct ttcagactgg ttacgtttcc ctgctctttg tgccctgact gacatactcg
     1201 cctcctgtta atgtgtgaga gaaatttacc aaaaaccctt ataaatcaat aacttttata
     1261 aattcccttg aacaattact tccggttttt atttttttta atcataaaaa atgcaatatt
     1321 cattacacaa aaggcggtta caagtaataa atattacaaa cacaaaatgc atttatatga
     1381 tttaaaaaga gtttttattt aattgcggtg ctccatgtgc tcatcgtgct ccatgtgctt
     1441 ctggtgctcc cttgtgctcc atggggttgt agggggcaag gaggggggga gaaatcgaaa
     1501 aataaattaa gagtcaccat gctggttact cgcatttacc ataacatccg ttgcgtgcac
     1561 cgccgcagag gccctcagcg cgtttctgag gcctccggcc tgaaacacac acctttctgt
     1621 gcccatatcg agaaaaaacg cccctgatga ttctggcgcg actgcatggc tatgcacgga
     1681 catacctggc cgggatttta gcggctaaaa tccgatgaac gccctgaaag ccaccacgaa
     1741 ccaccccgat aacggtgaac ggatgcgcaa tttattgcgt ggccgtgagg cgttgccggc
     1801 acagccggaa aaaccatttt cagcacgtgc aggaggccca ggaagagggg cggatgtacg
     1861 aatagttcag aggtcagatc tgacgtaaaa atgatgtaaa agtgacgtaa aagtgatgta
     1921 attttgatgt acttttgatg taattctgta gtcaggaaga ataacccgtt catgcaggca
     1981 tgaacgatac agtgaggaac gctttttttg tcacgatctc cccttaacct gcgtaaatca
     2041 ccctccatag ttgattattt ttcaacctta aaatggtcat acaccatacg caaccgcgat
     2101 tttcatcgca attgcatgaa ttaaaaggta aaaactgaag ttcccgttta tttatgtaat
     2161 acacaacgta ttacaaccac ctgtaatcac atgaaaataa agaaataaga tgtaataccg
     2221 gcgtattaca cgtacgcggg taagcgaaaa agctataacg cttcacggcc gacaggtggc
     2281 ccaaaatcca ccggggcgtg caggttctct tcagtaacct gcgccattaa atcaaaaagg
     2341 tcatattcgc cgggaagcag agcatcagtc gcatcccgtt gttcatcata ctcagatggc
     2401 atgttgtgat atccagtagc tgaggggtgt tagcgggttt cggggcgcag ccctgaacca
     2461 gtcacgcagc gctagcggag tgtatactag cttaaccatg cggcatcagt gcggattgta
     2521 tgaaaagtac gccatgccgg gtgtgaaatg ccgcacagat gcgtaaggag aaaatgcatg
     2581 tccaggcgct tttccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
     2641 cgagcggtac tgactcacac aaaaacggta acacagttat ccacagaatc aggggataag
     2701 gccggaaaga acatgtgagc aaaagaccag gaacaggagg aaggccgcgc cgctggcgtt
     2761 tttccatagg ctccgccccc ctgacgagca tcacaaaaac cgacgctcaa gtcagaggtg
     2821 gcgaaacccg acaggactta aagataccag gcgtttcccc ctggtgactc cctcgtgcgc
     2881 tctcctgttc ctgccattcg gtttaccggt gtcactccgc tgttatggcc gcgtttgtct
     2941 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     3001 cgaacccccc gttcagtccg accgctgcgc cttttccggt aactatcaac ttgagtccaa
     3061 cccggaaaga cacgacaaaa cgccaatggc agcagccact ggtaactgat ttagaggagt
     3121 tagtcttgaa gttatgcgcc tgttaaggct aaactgaaag aacagatttg gttactgcgc
     3181 tcctcataag ccagttacct tggttcacag agcgccaact cttagaaccg tcgaaaaacc
     3241 gcccgccaag gcggtttttt cgtttacaga gcaagagatt acgacgagag catcacaatc
     3301 tcaagaagat cttttcttaa catacaatac aactgtatct catctatttt tgactcttcc
     3361 agaagcgatg atctctatca aaaaacacga tatagaacac attatctctc agaaatccgg
     3421 cgactcttct tttctgcccg ccaatgtttt ttataacccc ccaatcctca tcagaggata
     3481 tctcatctgg gcatctaaaa tcgtttacag ctgactcagg aaatcgagtg tagttagtaa
     3541 acacaccgtc agaaatcact ttagtgattc cattcgccgt tagataaacc aatttttcaa
     3601 tgagatcagg taggctacca tcatctttcc atgtatttat ggactgccca acatcatcga
     3661 cgttatgaaa atgcttaaaa ctaatagata taaatctatc agtcgttttc gctgtgctct
     3721 ttgaaggttt tgtttcctca ttcctcgaag agcttcgaat gcttttgcct ctctttggca
     3781 tataatcatc cctcaggcat tagcttttca aattcattaa gattatcaaa taacgggatt
     3841 gcatcaaaag cacctgataa ataatcattc ccatatgatg gcttcaatga tctttgtttc
     3901 tctttgtact caaccaaaga ataaagatgg ctactccctg actttacatc cttgtcaaca
     3961 aaccaaattt ggtctcgtct aaagcaatca gtattcatta aattaacatc ctgaactgca
     4021 gcgattactt ggctaaaccc ttcactttgc gcatgaaata tcctaaataa aaacttagat
     4081 aaaagggtat ggaactttga atcgaactca tcaaccaata ataagttatt gttaatcatg
     4141 gcgttataaa taggcccaag gagatagact atctttttgg tcccctctga ttcaaaatca
     4201 atgggaaccg aaatctcccc attagcagtt tttttcacga ctctaagaga aaatactttt
     4261 tgattttttg caaaattctg aatcgagctg aataaatttt ttaaattatc atcatctgtt
     4321 tttatgttgc caaacggaaa ttcaggcgca acttcatcaa gataaatatc attaatctga
     4381 aaggctggta atattttttt tagccaagct ttaaaatctt cagattcatt tataagcttc
     4441 atggtaattc cagtaaacat tccttcgtta gaaccagaaa gaaataccac attactaaac
     4501 caattcacaa cacgctttga gtgctcccca ttaaatccag caacaacaga aacaaaggga
     4561 acatcttctc ttgtcttctg aatgacccca tcctttataa aaagatttga ctcgctaaat
     4621 gctgctttat ttatttcaat aagttgacca tctcgctcaa agagaatagt ctcccttgtt
     4681 tttggcgtgt agtacaacca ctcttctttt atcttccctt gaaataaaga taatccgtat
     4741 ctgtatttaa tcccatccgc aataaaagtc atttccattt cagatggttt agtaggcccc
     4801 gcctcatcaa gtaaaaaagg gactacattc ttcgtaatgc cactttcgat agcttgcaca
     4861 gaatgcagga caatatcttt cattaaagat attgtcttca tgagattaga ttttccgcta
     4921 gcgttagctc cgaaaatcaa agcagacttc agtatccgct catcccttac tgacactaca
     4981 ttagccaagt ttatatcgtc tttcgatgac gacaccgcca acatgctcat attttgagct
     5041 tccatgaagg aaaatgcgtt tgaaagacta aagttaacca tcataagaac acccctaaca
     5101 acacccttaa actcaaaaat tgcacaatat tgacaaaatt ctagattaag aatagcataa
     5161 ccccgtacta aaacaacaaa agggtccaat agacagcaca ttatgagtag ataagttaac
     5221 gaccagctta aaaagaggtg tatatgaagt tttttctata ccaaatggtc ccatttgcct
     5281 cagtgattag ctcagcagtg ctggcttttc actcggtatc tgggtgggga tggttccttt
     5341 ttgtcgcgtt tatgctcagt ggcgctgaca ctgtaaaaaa atacgtaggg agtgaaacat
     5401 gaatgaggag aagcaaaaat tgttgaagta agtgggttag cctgtaccac cagaactaac
     5461 ccgtaacaaa ccatacgcaa atgcggcgct tgagtaccac ctcaagtgct ttgataaagc
     5521 cagccacaag atctgttggt tgctacagta caattttgat agcaaaaatc aacattcatg
     5581 tggcgaaaat aggaaatgca acatgcagac ggacgagcct caggcataca tttttcgccc
     5641 ttacataacg gtaaagggca aacggataac ccgtcctaac gggggaatgt ttaagatccc
     5701 aattaatcgg gaaaagaaaa aacaataacc accgaagcat gcaagcttcg gtccctaact
     5761 tagtttatgt aaggaaagca acatggcctt ttatgtagat agcacagcga aatttttaac
     5821 aaaaacaacc gagcctgaag ggagtgtatg gcggtattac tataagccgg gaaatacttg
     5881 tccggcttcc ggcatttacc gctgcacgaa ctgtggtgat gaagtgacgt gcaataaaga
     5941 tgatcctttg ccaccgcaaa atcataggca gcataatgtc ccaggtgaca tccgatggga
     6001 attggtagtg atgacaaaga cgaagtaact tactaggccg tctacggacg gcttttttat
     6061 acctgccaat cagctggatt taccataaca tccgattgcg cgcaccgcta aaacgcgctg
     6121 aacgcgattc tgccgcaaac accgcaaaaa gagatgtttt ccccggggta aaccggaaaa
     6181 atgcgtcagg ctcgctttca gctcactgca tagctatgca tgaaagtgaa tgacgatcgg
     6241 tttgggcgca tacagtgccc atgagacaca ttttcggaca ttttcagttc gtgaatccct
     6301 gactcattgc tacatatctg aacagacacc cctggcagcg cccccagggg cgtcctgtaa
     6361 aaaaacacac cgcgcagcta gcagcccccc ctaatataag atgatgtttt ttatgaaaat
     6421 agtcagtacc acccctaaaa agcggtgtcg gcgcggtgtt gtagctgcgc caacaccgct
     6481 tttttaaata tcacaaagag agcaagaaga actaatttgt cataactctc tatttataaa
     6541 gaaaaatcag caaaaaattg tttttgcgtg gggtgtgggc atttcgggca tgagaacaga
     6601 ctcgcgtttt tccggagtat cagcggaatt attgattaag aaacactcga cgcagagcga
     6661 aattttatgc aacagcggct gcactgcaca gggattattt atcagttgat atgctgtgcc
     6721 atgatacgtc ctaaagaaac aggacaggat gtgcaattgt aataccgtcg cccgcaacgt
     6781 cttacaatta ccacctgctc agggtccccc cgacacccgg aactgacgga gaacaacgga
     6841 aggtgaacaa acaacaacaa actgcgctga atatggcgcg atttatcaaa agccagagcc
     6901 tgacactgct tgaaaaactg gatgctctgg atgccgacga acaggcggcc atgtgcgaac
     6961 gactgcacga actcgcagaa gaactccaga acagcatcca gattcgcttt gaggccgaaa
     7021 gtgaaacagg aacataacga agctgacgga ggcggtcacg gctttcccgt gggcgttttc
     7081 gtcagcagac aagcccgtca gggcgcgtca gggggtttcg gggcgaagcc ctgaaccagt
     7141 cacgtagcga tagcggagtg tatactagct tagtatgtta cgacggggtg agggttcagg
     7201 aaagtgcttc atgtggcagg agaaaaatgc agcagcggag cgtcagcaga atatgtgata
     7261 caggatatat tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7321 agcggtacca acttacaggc ggggcggaaa tttcctggga gatgccagga agaagcttaa
     7381 cggagaagcg ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag
     7441 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact taaagatacc
     7501 aggcgtttcc ccctggtggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     7561 gtgtcattcc gctgttatgg ccgcgtttat ctcattccac gcctgacact cagttccggg
     7621 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactaccac
     7681 gcccgttccg gtaactatca acttgagtcc aacccggaaa gacacgacaa atcgccagtg
     7741 gcggtagcca ttggtaacgg atttagagga gttagtcttg aagttatgcg cctgttaagg
     7801 ctaaactgaa aggacagatt tggttactgc gctcctccaa gccagttacc gcggctcaaa
     7861 gagttggtag ctctgagaac cttcgaaaaa ccgccttgca gggcggtttt ttcgtttaca
     7921 ggacaagaga ctacgacgat agacaaagga tctcaagaag atcatgataa a
//
