LOCUS       CP057412               28651 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB27-C20 plasmid pRHB27-C20_5, complete
            sequence.
ACCESSION   CP057412
VERSION     CP057412.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148263
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28651)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 28651)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH04 T2-C20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 146.358368757903x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:24:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,633
            CDSs (total)                      :: 5,500
            Genes (coding)                    :: 5,127
            CDSs (with protein)               :: 5,127
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 373
            CDSs (without protein)            :: 373
            Pseudo Genes (ambiguous residues) :: 0 of 373
            Pseudo Genes (frameshifted)       :: 136 of 373
            Pseudo Genes (incomplete)         :: 211 of 373
            Pseudo Genes (internal stop)      :: 61 of 373
            Pseudo Genes (multiple problems)  :: 34 of 373
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..28651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB27-C20"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB27-C20_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            1..870
                     /locus_tag="HVY46_27835"
     CDS             1..870
                     /locus_tag="HVY46_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMG97733.1"
                     /translation="MRFQVRTVTGLTKVRHRNEVGVTLASLSLSAKRVLFLALCQIDSK
                     EMLNDEILEVDADLFSKTTTLDKYAAYAALKEGAKVLASTTLVLNRDDLKNLSNELGIA
                     TSKNKMPDRLDLNLTEFCAYYDHLAIVRIKFTNTAKRYFSKLIGSENRYTTQVLKSVVI
                     LNSVNSTNLYQVIRKYYSLNPASKSFEISVDQLKDEMGLYHIENGEKVYKYPKYSFFIR
                     DVINKSIKEIIEKTEIKTIKFTVVGKKGRMVHMLKFEYSLNEDTSISDEDAKFLEKFDK
                     VISYKQKG"
                     /name="replication initiation protein"
     gene            875..1726
                     /locus_tag="HVY46_27840"
     CDS             875..1726
                     /locus_tag="HVY46_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMG97734.1"
                     /translation="MEQIKLHKDSNVSIIVIPNHLLKNTGWDDGTLLDVVKSDNALHIR
                     RKEDVVDLVTKPCVDILTATFKNIKPGKIIKYNSKLNESLNKLHVSARRVFFICLYLYF
                     NGRYNSDGSILIRADKYAEFIGIDRTMAYKQMKDAADYFSSNIKLISLCDYIKNEGLLR
                     VALSTETINFISAVDGRKYQTTVVLYQSAVKLSGRYSWNLYQLIKSRLLDKSGAFSIKL
                     DELMIELNSRVNLEFKDYKKSVIGRSIDEIVEKTEIKSIKCVNAERQGRRVSKVRFEIE
                     MR"
                     /name="replication initiation protein"
     gene            1730..2173
                     /locus_tag="HVY46_27845"
     CDS             1730..2173
                     /locus_tag="HVY46_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97735.1"
                     /translation="MAKIYQFPQGEERAKFRKEIARERKKRFAVKTGSTFVKWLGWGWF
                     YLRLLAASVLHFIFVFVLAILGAFKWAVFWLGGILCVITWYHLDHQLWTPQNLTIPVIT
                     ATWIFGLLAEPLMELLNKTMPWHKLLVPEPAGESIENSSETSQ"
                     /name="hypothetical protein"
     gene            2244..2345
                     /locus_tag="HVY46_27850"
                     /pseudo=""
     CDS             2244..2345
                     /locus_tag="HVY46_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694628.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /name="DNA distortion polypeptide 3"
     gene            complement(2666..3643)
                     /locus_tag="HVY46_27855"
                     /pseudo=""
     CDS             complement(2666..3643)
                     /locus_tag="HVY46_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(3668..3949)
                     /locus_tag="HVY46_27860"
     CDS             complement(3668..3949)
                     /locus_tag="HVY46_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="QMG97736.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFRL
                     SAHFEDILEAEAIRTGQSKTTVLKAALAMYNSQDENIKNHWLLESAKF"
                     /name="CopG family transcriptional regulator"
     gene            complement(4047..4709)
                     /locus_tag="HVY46_27865"
     CDS             complement(4047..4709)
                     /locus_tag="HVY46_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-arginine deiminase"
                     /protein_id="QMG97737.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHDL
                     LSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVSDI
                     VVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDLDKL
                     LRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIGIL"
                     /name="peptidyl-arginine deiminase"
     gene            5090..5734
                     /locus_tag="HVY46_27870"
     CDS             5090..5734
                     /locus_tag="HVY46_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QMG97738.1"
                     /translation="MTSNAPTSFVRAYLRASTAEQDASRAQETIEDFARERGLIICSFY
                     IENESGSRLDRPELFRLLRDCQQNDILLIEDVDRLSRLSGEDWNTLKKMIRQKDIRVMA
                     VNVPTTWINSGMSEFDSRLFAAINDMLLDMLAAVARRDYEQRRERQKQGIEKARKDGKY
                     KGRKPNQARHDAIIRLIESGSSWTQVQKVLGCSRGTISSAIKRKTGQKSSK"
                     /name="recombinase family protein"
     gene            complement(5889..5972)
                     /locus_tag="HVY46_27875"
     CDS             complement(5889..5972)
                     /locus_tag="HVY46_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="QMG97765.1"
                     /translation="MIDKAIIVLGALISLLELIRYLLQLMG"
                     /name="Damage inducible protein"
     gene            complement(6966..7595)
                     /locus_tag="HVY46_27880"
     CDS             complement(6966..7595)
                     /locus_tag="HVY46_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97739.1"
                     /translation="MLEQFFDILNKDVLFDLSKTRTPDSSKLIIHEDGELSEIKELEIK
                     EIPDDSIAFTLDFSSKRNVKKNGVLFSQLSPYFDKGNGDGVNKSCDLVLISRNGDKFTI
                     LIFDQKSKKPDIDSSFIQLENSRLFISYMVDIISLFYDVNVNKDDITFKRVIGTTRVVK
                     TGVNAATSQQEILRRKKLRELGMKEVSIKRTASPEKGWLDYRGIVL"
                     /name="hypothetical protein"
     gene            complement(7597..9048)
                     /locus_tag="HVY46_27885"
     CDS             complement(7597..9048)
                     /locus_tag="HVY46_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="QMG97740.1"
                     /translation="MEIEFENIGFIKSGKIETNNITIIFGPNNVGKTYLSYSTYAAISK
                     YNAILKGKIKIPDSVSSDLVLNGVAKYDIANVFTDGFIKSVNDELSSKLPAFFKDSSEM
                     FKNSKIRVIDSHIKDRVVNAEVNINVSISENIILNIKKEKNSTSIIFSVASSKNNNEAG
                     KYVVNLKEKDASDHLNILFNFFFVREILDSAANGTFIITSERTGISLFQKEIDGNRSNI
                     VDSYIWNRLSGLSSSDFVWAFDDIMNKKVAKFSEPINHNINIIRNFSGLNEKTAFMTTT
                     ANRGRRSPRKSAELADIIRTLESLTGGQYSPSDGDTIFTASCGNGDKVSMPLSMSSASN
                     KALFLLDIYIRYYLSNDSYLIIDEPELNLHPHNQIKMAELLVRLSNYGVKIIITTHSDY
                     IVKEINNRIMASQLKDRAQLDEFGYKKSDVIKPSEVNGFTIDNDGIIREVKGNKYGVNA
                     FIFDNAISDIENRTERLYTYLEGDL"
                     /name="AAA family ATPase"
     gene            complement(10230..10976)
                     /locus_tag="HVY46_27890"
     CDS             complement(10230..10976)
                     /locus_tag="HVY46_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="QMG97741.1"
                     /translation="MSAISLLEQAAKKELNYREFLCMALQQEWNGRHQRGMESRLKQAR
                     LPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVILLGPPGVGKTHLAIAPGVKA
                     VDAGHRVLFMPLDRQIATLMKAKQENRLERQLQQLSYARVLILDEIGYLPMNREEASLF
                     FRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAILDRLLHHSTTLNIKGESYRLKE
                     KRKAGVLTKNTTPISDDEMVKSGQHQ"
                     /name="ATP-binding protein"
     gene            11395..11631
                     /locus_tag="HVY46_27895"
     CDS             11395..11631
                     /locus_tag="HVY46_27895"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97742.1"
                     /translation="MTAIKSNSKHTKGMSSSDEDNRKLFNLRQPNACGNNPKAVIGGTI
                     MLPLLNTFMNAPLLSQGATILFLGLLILALKKQ"
                     /name="hypothetical protein"
     gene            complement(11728..12177)
                     /locus_tag="HVY46_27900"
     CDS             complement(11728..12177)
                     /locus_tag="HVY46_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97743.1"
                     /translation="MVKFPGEVMSSSAAVTVITGVLVFVGGQLIVKSAIEPYIEFKKQL
                     GKISNLLLANQAKLANPCAVEMSEIIHELKDAAAQLMSKHSALPFYIKKFHIGFRFVPS
                     TPEIISSAQKLNLIASIHEGKSKESTYEHMAEIGRMLKIPTTYSL"
                     /name="hypothetical protein"
     gene            12322..13098
                     /locus_tag="HVY46_27905"
     CDS             12322..13098
                     /locus_tag="HVY46_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97744.1"
                     /translation="MELDTEFRYPNVIDFFGTTLFHLCHFTTIDLHNYAAYNKYAENNI
                     AKSNKRICEEIEQRIKESDKKYHEDIIDSYHQEMQEFGDIYPPMHRKSMVITLYNFCEH
                     QIKTLCLEINKLLPEDMCVKYKLKDDICIKKYRKFLRREAGFDIHPSAELWNLWENMLK
                     VEQIRHVLVHSEGEIEKHRAERLADIKSYCNKKENIRLNKHKIIIDEGYVAGLITELIS
                     LFELLDRQVNLFIRRYESKHGSYEVPLPQEASRTAL"
                     /name="hypothetical protein"
     gene            13157..13609
                     /locus_tag="HVY46_27910"
     CDS             13157..13609
                     /locus_tag="HVY46_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000374058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97745.1"
                     /translation="MDRIEYLKWLMDESPSTTQQLMAWLQRAKCYTPEMKEHRDGVQIE
                     ENGIIVGLRQRTNLYNGDCLTIHVVQLPEKIQNKGWFKSFLKLCCESNPWNDVVIEDVK
                     NPYLLAFCQKHKFKVLADFYPDTYIVNSEEIMALEIPPLKRYEDYL"
                     /name="hypothetical protein"
     gene            complement(13681..13860)
                     /locus_tag="HVY46_27915"
     CDS             complement(13681..13860)
                     /locus_tag="HVY46_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97746.1"
                     /translation="MDFMSILGRCLLLPFPVAMFVGCLFPGIDDRFTGMFMSFIVGCLS
                     WYITKPKPQKAEVA"
                     /name="hypothetical protein"
     gene            14850..15200
                     /locus_tag="HVY46_27920"
                     /pseudo=""
     CDS             14850..15200
                     /locus_tag="HVY46_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156234.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            15253..16466
                     /locus_tag="HVY46_27925"
     CDS             join(15253..15544,15544..16466)
                     /locus_tag="HVY46_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="QMG97747.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTPE
                     TLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFGEGGVRP
                     PLEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLKKEI
                     QRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVSRKAV
                     AAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTF
                     VLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMAES
                     INGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEAEKAYYASIGN
                     DDLAA"
                     /name="IS3 family transposase"
     misc_feature    15534..15650
                     /locus_tag="HVY46_27925"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(16508..16864)
                     /locus_tag="HVY46_27930"
                     /pseudo=""
     CDS             complement(16508..16864)
                     /locus_tag="HVY46_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247738.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /name="VirB3 family type IV secretion system protein"
     gene            complement(16883..17179)
                     /locus_tag="HVY46_27935"
     CDS             complement(16883..17179)
                     /locus_tag="HVY46_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="QMG97748.1"
                     /translation="MLKLNLNKRYLTLSLFMAALMLCVAEPAFADDVSTKTTGFLQKII
                     DFLTDIRKPAITIIALVIGYIAIFSRQHTSWIVPLVIGIIIFIVAPYIPDWLA"
                     /name="TrbC/VirB2 family protein"
     gene            complement(17157..17804)
                     /locus_tag="HVY46_27940"
     CDS             complement(17157..17804)
                     /locus_tag="HVY46_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="QMG97749.1"
                     /translation="MLSTSTFLALAMQCAASVHPDTTHEVARVESGFNPYAIAEIIPKV
                     KRKPGNKGVVSYFPESKEAALKIVKNIELRNHRYSVGLMQITSTNFAKFGTTAEKMFDP
                     CENLKVSEKILVDCYKRGGDLVRGLSCYYSGNPETGVKPEPEFNNTSYVQRIGFSPPDN
                     KKSFIVPSVKEMIKKENKTTITPEEIIIYPQYAMRGTVSNEKETKDVEIKSE"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(17978..18496)
                     /locus_tag="HVY46_27945"
     CDS             complement(17978..18496)
                     /locus_tag="HVY46_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="QMG97750.1"
                     /translation="MKGQKQFYLLQCSPHFHQSVLLQLSRSGIAYYNPVIRTFYKRRDC
                     SAYRGQIMPMFPGYVFVLLDFEVVHPSRFTRMKNVYGLVSFGEYPAEVPLSVINEVKEQ
                     EKIFSMNLSLMNNSCLAKILLLAEASKRGQVFADYVCNKKYDRLKNDSYKRKAETATQC
                     TLPSYRSSC"
                     /name="transcription termination factor NusG"
     gene            complement(18849..20015)
                     /locus_tag="HVY46_27950"
     CDS             complement(18849..20015)
                     /locus_tag="HVY46_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="QMG97751.1"
                     /translation="MGVYVDKEYRVKRKSSENGRKSAFAHKVKNGGKNYSRNVQERINR
                     KGASKEVVVKISGGAVTRQGIRNSIDYMSRESELPVMSESGRVWTGDEILEAKEHMIDR
                     ANDPQHVMNDKGKENKKITQNIVFSPPVSAKVKPEDLLESVRKTMQKKYPNHRFVLGYH
                     CDKKEHPHVHVVFRIRDNDGKRADIRKKDLREIRTGFCEELKLKGYDVKATHKQQHGLN
                     QSVKDAHNTAPKRQKGVYEVVDVGYDHYQNDKTKSKQHFIKLKTLNKGVEKTYWGADFG
                     DLCSRENVKAGDLVRLKKLGQKEVKIPALDKNGVQHGWKTVHRNEWQLENLGVKGVDRT
                     PSASKELVLNSPDMLLKQQQRMAQFTQQKASTLQSEQKLKTGIKFWGL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(20018..20563)
                     /locus_tag="HVY46_27955"
     CDS             complement(20018..20563)
                     /locus_tag="HVY46_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 1"
                     /protein_id="QMG97766.1"
                     /translation="MKKVQFRIDENQHDDLLDCLKTLYPDEPALTVAKGMKLLANALLK
                     SKAGSKDINTFFDNNDFIKTTMYLTGKQRADIERAANRHGWTLSRECRYRIQTTLENEL
                     DFFDQELLMMNRCRNSIDKIGRNFHYIIVNDQTRVLDKDGFYQDVERLTKEIFNLKNQF
                     ENYIMLCKGRTVSNKVEM"
                     /name="DNA distortion polypeptide 1"
     gene            complement(20558..20632)
                     /locus_tag="HVY46_27960"
                     /pseudo=""
     CDS             complement(20558..20632)
                     /locus_tag="HVY46_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202958.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /name="DNA distortion polypeptide 3"
     gene            complement(20889..21161)
                     /locus_tag="HVY46_27965"
     CDS             complement(20889..21161)
                     /locus_tag="HVY46_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97752.1"
                     /translation="MTKHDFVSFVSGELRQGAVRFSLAFNSKGEIVLHWTNKAGIRVWR
                     ILSGNRGKKPSKANLERMSNFRRWLFDARQGMEGYTQQPEQSNLS"
                     /name="hypothetical protein"
     gene            complement(21174..21323)
                     /locus_tag="HVY46_27970"
     CDS             complement(21174..21323)
                     /locus_tag="HVY46_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97753.1"
                     /translation="MSERILSAINDVEKGGRPVFPLMPFHVFPEYMALLRKALEKKTQK
                     RTDK"
                     /name="hypothetical protein"
     gene            complement(21393..21572)
                     /locus_tag="HVY46_27975"
     CDS             complement(21393..21572)
                     /locus_tag="HVY46_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97754.1"
                     /translation="MEREFSAKASLNRNIKFWFEQCGLSKERVIRCIDNWYDLAYPPSE
                     QEKAKKEAIEKLIK"
                     /name="hypothetical protein"
     gene            complement(21634..23205)
                     /locus_tag="HVY46_27980"
     CDS             complement(21634..23205)
                     /locus_tag="HVY46_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="QMG97755.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEIN
                     RLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPSAL
                     RQSSARKPLPASLPRETRVIRPEEECCPACGGDLSPLGCDVSEQLELISSAFKVIETQR
                     PKLACCRCDHIVQATVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQGVEL
                     SRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTARLWVYV
                     RDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAA
                     CMAHARRKIHDVHARVPTDITTEALQRIGELYVIEAEVRGCSAEQRLAARKARAAPLMQ
                     SLYDWIQEQMKTLSRHSDTAKAFAYLLKQWEALNVYCSNGWVEIDNNIAENALRGVAVG
                     RKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPANRVRDLLPWK
                     VDLTSQ"
                     /name="IS66 family transposase"
     gene            complement(23225..23572)
                     /gene="tnpB"
                     /locus_tag="HVY46_27985"
     CDS             complement(23225..23572)
                     /gene="tnpB"
                     /locus_tag="HVY46_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="QMG97756.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLFI
                     FRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDWKH
                     PKRTERAGIRI"
                     /name="tnpB"
     gene            complement(23572..24222)
                     /locus_tag="HVY46_27990"
     CDS             complement(23572..24222)
                     /locus_tag="HVY46_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="QMG97757.1"
                     /translation="MNARKAVLADNPELILRVLQLRFDESLSYPRISAQTGVSKTTIFS
                     LVRRFHQVFTDWPLSGEYSCGQLARALFPGRYPSAPTVTQPVKAEKPRRNRFSPEFKWR
                     LVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPPGEHETSLLPVTLTPEPDN
                     KIPAPVQIPEQINTLPDSLCCELVLPAGTLRLKGELTPALLQTLIREMKGSSH"
                     /name="IS66 family insertion sequence hypothetical protein"
     gene            24371..25483
                     /locus_tag="HVY46_27995"
     CDS             24371..25483
                     /locus_tag="HVY46_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
                     /protein_id="QMG97758.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLLR
                     LGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAVTG
                     CTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFAQTA
                     DEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGLDCGK
                     NGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQAETLEA
                     AGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPELAKAWIF
                     ANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
                     /name="IS4-like element IS421 family transposase"
     gene            complement(25677..26006)
                     /locus_tag="HVY46_28000"
     CDS             complement(25677..26006)
                     /locus_tag="HVY46_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97759.1"
                     /translation="MKVLTFKNDTVSVGDVFVSSWGYEQTNVTFYQVLSVHGKKTVTVR
                     EIRANSEYTHSMVGFKTPVLNDFTGECLKRQIKDFGDELAIKIEDFETAYKTLPEEKHR
                     FSSYY"
                     /name="hypothetical protein"
     gene            complement(26099..26314)
                     /locus_tag="HVY46_28005"
     CDS             complement(26099..26314)
                     /locus_tag="HVY46_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97760.1"
                     /translation="MQNKPTPEEVKNARVAAGLTLKEAADIFGYQLNSWQMKESAGKAS
                     RSLSVGEYQYLLLLANMHPSYRLVKK"
                     /name="hypothetical protein"
     gene            complement(26304..26549)
                     /locus_tag="HVY46_28010"
     CDS             complement(26304..26549)
                     /locus_tag="HVY46_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97761.1"
                     /translation="MNKDKIIKKNRGNYSYVIRTMDEDGDAVFHVLKYVKTIDKTKNRK
                     TVRKLIMDEKLNLASLMLLDNGILCDCLTKGDENAE"
                     /name="hypothetical protein"
     gene            complement(26597..26920)
                     /locus_tag="HVY46_28015"
     CDS             complement(26597..26920)
                     /locus_tag="HVY46_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG97762.1"
                     /translation="MQNYAKSVATEILRQLGGNRFIVMTGAKSFSYFDENGECGLTFRL
                     PSNFAMKGINLVKIKLDFTDTYQVKFSRVRGDEVKDISRFDNIYCDQLACLFTQETGLH
                     TVL"
                     /name="hypothetical protein"
     gene            complement(27065..27346)
                     /locus_tag="HVY46_28020"
     CDS             complement(27065..27346)
                     /locus_tag="HVY46_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QMG97763.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADLR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(27336..27587)
                     /locus_tag="HVY46_28025"
     CDS             complement(27336..27587)
                     /locus_tag="HVY46_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QMG97764.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
                     /name="plasmid stabilization protein"
     Typing          27956..28545
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.985"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1731..2176
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.302"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(18853..20015)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.969"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaggtttc aggtgagaac tgtaacaggg ttaacgaaag tcaggcatag aaatgaggtt
       61 ggcgttactc tggcatctct ttctctttca gcgaaaagag tcttgtttct tgcactttgt
      121 cagattgact caaaagaaat gttgaatgat gaaattttag aagttgatgc cgatcttttt
      181 tctaaaacaa ccactttgga taagtacgcc gcatatgcag cattaaaaga aggtgcaaaa
      241 gttctggctt caacaacatt agtgttgaat agagatgatt taaaaaatct atctaatgaa
      301 ctgggtatcg ctacatctaa aaataagatg cctgacagac tggatttaaa cttaactgaa
      361 ttctgtgctt attatgatca tctggcaata gttcgcatta agttcactaa tacagcgaaa
      421 cgatattttt caaaacttat tggttctgaa aatagatata ccacacaggt gttaaaatct
      481 gttgttatat tgaacagtgt aaactccaca aacttgtatc aggttatcag aaaatactat
      541 agcttaaatc ctgcgagtaa atccttcgaa atttcagttg atcaacttaa agacgaaatg
      601 ggactttatc atattgaaaa tggtgagaag gtatacaaat atccaaagta ctcttttttt
      661 attcgtgatg ttataaataa aagtataaag gaaataatag aaaaaacaga gataaaaact
      721 ataaagttta ccgttgtagg aaaaaaaggg cgtatggttc acatgttaaa atttgaatat
      781 tctcttaatg aagacacgtc tatttctgat gaagatgcta aattcttgga gaagtttgat
      841 aaggtaattt catataaaca gaaaggataa tattatggaa caaataaaac tacacaaaga
      901 tagtaatgtt tcaataatcg ttattccaaa tcatttactc aaaaataccg gatgggatga
      961 tggtacgtta ttggatgtgg taaaatcaga taatgcactt catattagaa gaaaggaaga
     1021 cgtcgtagac ttagtcacta aaccttgcgt ggatatttta accgctacgt ttaaaaatat
     1081 taaacctgga aaaattataa agtataatag caagctgaat gaaagtctta ataagctcca
     1141 cgtctcagca aggagagtct tttttatttg tttgtatctt tattttaatg gtaggtataa
     1201 ctcagatggg tctattttaa tcagagctga taagtatgct gaatttattg gtatagatcg
     1261 cacaatggca tacaaacaga tgaaagacgc agcagattat ttttcttcca atataaaatt
     1321 aatatctctt tgtgattata taaaaaatga agggctatta agagtagctc tttctactga
     1381 gacaataaat tttatttctg ctgtagatgg tagaaaatat caaactacag ttgtattgta
     1441 tcagtctgct gtaaaactca gtggtcgata ttcatggaat ttatatcagt tgataaaatc
     1501 tcggctatta gataaatccg gtgcttttag cattaaactg gatgaattaa tgattgaatt
     1561 gaactctaga gtaaatttgg aatttaaaga ttataaaaaa tcagttattg gtcggtctat
     1621 agacgaaatt gtagaaaaaa cagaaattaa aagtattaaa tgcgttaatg ctgagaggca
     1681 gggtagaaga gtcagcaaag tccgttttga gatcgaaatg aggtaactca tggctaaaat
     1741 ctatcaattc ccccaggggg aagaacgtgc taaattcagg aaagaaatag ccagggagcg
     1801 taagaaacgt tttgcagtga aaacagggag cacttttgtt aagtggcttg gctggggatg
     1861 gttttatctt cgtcttcttg ccgcgagtgt tttacatttt atctttgttt ttgtgttagc
     1921 cattcttggt gcgtttaaat gggcagtgtt ctggctgggt ggtattttgt gtgtcatcac
     1981 ctggtatcat cttgatcacc agttatggac gccacaaaat ctgactatac cggtgattac
     2041 ggcaacctgg atttttggtc tgttggctga accgctgatg gagctgctca acaaaaccat
     2101 gccctggcac aagttactgg tccccgaacc tgcgggcgaa agcatagaga acagcagcga
     2161 gacgagccag taatgacgct acttagtcgt tatgccagct cctgcctttt cactgcgatg
     2221 caggataggg caaacgccgc aaaatgacgt ctctgacgcc attccgcaac gtttgcccgg
     2281 tgacctacag tcggttcttg ttggagaatt ttatgaaata tcaggtgaga gagtttatta
     2341 acgaaaaata tgctaaggct gttaatattt taaatgataa tcttaaagaa aacttccatg
     2401 ttttttatgg agtaagatta agtgagattc tttttccttc cagtgagtat ggctctgatg
     2461 cattctttaa agagtttgaa tcaattaaca gtgtaatact tccattagta atatttgatt
     2521 ttatagaccg aaagccgatt atggttatcg gcttcgataa gatacctgat gccagtttgt
     2581 tggaaggaac aaatattgtg gttcttgaat gtactaccct cgccgatctt ctgacaaatg
     2641 ataacattgg ttttttatat aaaagctaag aatctcctct cagtttatat tttctgcttg
     2701 ggttgccaag tgttatcgag tctagttcat gcgagtaatg atgtgtatga taatgtgctg
     2761 gatttttcag ataatcaggg agtgatatta tttttccgta gctaattgca gaataaaacc
     2821 agttgcgaaa tgtctcgctg cttgttttta aagcatcttt gggaataaat gctaatttta
     2881 tgttagaaac gtaattaaca ggtttaagtg tggtatgtct gttttgtcca aagtctttta
     2941 aagcatctgt aaattcatat tctgatgtat aattttccgt cttattacta ggttgtttta
     3001 tctggaatat gaatgaatag tagttctggc caagtatttc aacggtgttt gaatttatgc
     3061 tcagttgttc aataaccgct tctcttttat actctaatct gtctctgaca gattgatata
     3121 caaccattgc tgcatgatgt gaaccaccac tgtttaaagc tacatatcgg tcaagccagg
     3181 agtaatattt aacattaata ggatcgcgag agttgaaacc ttcatgctca atgtgccata
     3241 tattatcctc ccagtctgaa atgttttcag ctttcaggtt tttggctgga tacatcccct
     3301 ccccccacga ataaagatct ttgaaccaac taccatttat taccttgctc catccacaac
     3361 cgaaactttt cgagtcagtc agagattgta ttttgttaaa atcgaagtct agagagtgtt
     3421 tattatctgt agttattaag ttgctcagaa tttcagctag atccctgtca atatatctca
     3481 gtgagtattc gctaaatata ttttcaagct gcaatatggt gtctgcatct gctgtaggtt
     3541 taaataactt aatatcagca agatttttaa acgttgctgt ggtcagttta tggtgatctg
     3601 tcaacctgta gagtttacag attaattcgg ttaattatct catagatttt catccatgct
     3661 tctatggtta gaattttgca gactcaagca accagtgatt ctttatgttt tcatcctggc
     3721 tgttgtacat cgccagcgct gctttgagaa cggttgtttt gctctgccct gttctgatag
     3781 cttcagcttc cagtatgtcc tcaaaatggg ctgaaagtct gaagttcatc aactttggtt
     3841 tacccactac aggcgctctg gtagcgccgg agataaaacg cgcggtttct gcgtttgttg
     3901 ctggttgctg agattcatcg atgttcggac ggtttagttt taaagccatg acaatttccc
     3961 cttgtacggt atgcattaat agtaaatatc atgctgtctt tacattatct ttacaatata
     4021 aagatttata gatggtattg atattactat aaaattccaa tttcctgtgc cacaagttca
     4081 atctgtgctt tcgctgttga aaggcttgat gcccggctaa cgtcatgtac ccctgcccct
     4141 tcattacagg cgctttcata cacatccagc tcggatatcc gggttttaag aggttgaatc
     4201 cagatgttgt ccgaccgcag taatttatcc agatcaatgg ccgcttttct gtggcggggt
     4261 tttgctgagt tgattctggt gaacagcacc cacggttcca gggctgcgtt agctttctga
     4321 gcggtgcgga ttttctctgt aacactggtc agtgtttcgc tttcgaaatc agaggacggc
     4381 ttaaccagtg ttaccacgat atctgatact gtcagggcgc tacggaactc ctggctgtcg
     4441 tggccgggac aatcgacaat gaggacttca cacagcttac taagtcgctt aatttcattg
     4501 ctgacgtttc cgtaagctgc gtgaaccggg atagtgagta agccgttatc tgtccgcttt
     4561 tcgttccagc tcaacagatc gtggttttta tctgtcttga ggataattac cgacttacct
     4621 ttattgacca gcattgagcc tgtgttagcc acgtaggtac tttttcctac accaccctta
     4681 tctgatacga caagcaagat tttacccatt tgagaattcc tctctttata atgtaaagat
     4741 tatgtaaaga ccgtcttatc tttacattgt gtgttttccg taaagatgat tttgtcttta
     4801 cagtgtcttt atattacatg ggatttacca tgtaaagcac aaataaagac aatcatgtat
     4861 ttatgttgtc tttattttgt aaagactaat gtatatttac attggcttta catggtaaat
     4921 caacacttgt ctttacatca tctttatgtt gtaaattccc atgtgacatt gtcactttat
     4981 gaccagaggt ctgttttctg gttcttttag agtgtgcctt tcctatcgat ccactttctg
     5041 gtgcattttg ggtatagtgg tgtgacctta tgcgaactgg ttctgtaata tgacttcaaa
     5101 tgcaccaaca agctttgtcc gcgcttatct gagagcttca acagcagaac aggatgcttc
     5161 gcgtgcccag gaaactatcg aggattttgc cagggaaaga ggcctgatca tttgcagttt
     5221 ttatattgag aatgaatccg gctcccgtct tgaccgccca gaactcttcc gtcttctgag
     5281 agattgccag caaaatgaca ttttgcttat cgaggatgtg gacagacttt cccgcctgtc
     5341 aggggaagac tggaatactc tgaagaaaat gattcggcag aaagacatcc gggttatggc
     5401 ggtgaatgtg ccgacgacct ggattaattc tggtatgagt gagtttgaca gccggttgtt
     5461 tgctgccatt aacgatatgc tgctggatat gctggccgct gttgccagaa gggactatga
     5521 acaacgccgg gaacgccaga aacaagggat agagaaagca aggaaagacg gcaaatataa
     5581 agggcgtaaa cctaatcaag ccagacatga tgcaattatc aggttgatag aaagcggtag
     5641 ctcctggaca caggtacaga aagtgctagg gtgcagccgc ggcacaataa gcagtgcgat
     5701 aaaacgtaaa accgggcaga aatccagtaa gtagagggga ggggggttaa tatatctgaa
     5761 aggcaggata ttaactcagg aggttggggg tgtggtgttt agtcaatgca tttgaatgtt
     5821 gtaactagaa tatagtttta ttaaaggccg gaggtggtta gcccccggcc ttagttacgt
     5881 ctctgccatc agcccatcaa ctggagaagg tagcgaatca gttcaagaag cgagattagc
     5941 gctcccagaa cgatgattgc tttatcaatc atccgtttac tccattatat ggagcagact
     6001 cccgatactt actcttacac tgcctgtcat atcggttgtt cttcgatgtt ggttgaagac
     6061 agttccactc cagccagagc actgacaaca gcaccacctg tttttcagcc aggacttaaa
     6121 atcgcttaat ccttgcggga aggctggagt tataacggat cagaaccaac cggcgtatta
     6181 tacgcataag cggatacggt tgcattccat atcatgtaga catattatgt ctatgttggt
     6241 tgaagagagt tcttctccag tcagagccac caactcaagg ctggaaaaaa caaaaaagcc
     6301 cgattaccag tcgggttttt ttgtgcctgt cgttcggctg tacttccgtt ttttcccctt
     6361 catttgaggg atttttcttt cattttttgt tgcggccaat actgctgatc gcgttggcca
     6421 acagaacaat gccgttaccg gcattatcat gaatcccgtt ttttcagtaa gttgcagcat
     6481 acaaagtaat taaaaaaacg tcattttttg ttgatggatg cagtagcggt ggtgcgctac
     6541 gtgaaatctg gaaaatgctg gcgggtaagt gactcaattt ttaatctccg cacatgtttt
     6601 ttggacgtgc cagcaattat gacctgaagc agaggtgcgt ccgacaacaa aaattccgat
     6661 caccaaaaaa attttggaca ttttttccga taatgcattt ttgtccaggg ggatatgaac
     6721 gacagggcag gacgaaatct ggaagttatc ctgaattacc caggatatgg gcgcgtatgc
     6781 cggagtagag caccagataa aagaaatgaa ttcagtgtat gttgtaacta ataaggtctg
     6841 gacagaaaca cctgaatgtc caattataca gaaaattaaa cgaggccaga gacaaataat
     6901 ctccctgagg ggaggcatcc tgtatctctg gcaggttcac tttcagagat gaaacttgat
     6961 tatcatcaca atactattcc tctataatct aaccatccct tttctggtga tgccgtacgc
     7021 tttatggaca cttctttcat accaagttca cgaagcttct ttcttctcaa tatttcttgt
     7081 tgagaagtag ctgcattaac tcccgttttt acaactctgg tagttccaat tactcgttta
     7141 aaggttatat catccttatt aacgttaacg tcgtagaata aagaaataat atcaaccata
     7201 taggaaataa aaagccttga attttctaac tgaataaatg atgaatctat atcaggcttt
     7261 ttagattttt ggtcaaaaat aagaatagtg aacttatctc catttctgct tatcaataca
     7321 agatcgcatg atttatttac tccatctcca tttcccttat caaaatatgg tgacaattga
     7381 gaaaataaaa caccattctt tttaacgttt cttttactcg aaaagtccaa agtaaatgca
     7441 atcgaatcgt caggtatttc ttttatttcc aactctttta tttcactcag ttcaccatcc
     7501 tcatgaataa ttaatttaga agaatcagga gttctcgttt ttgacaaatc aaataaaaca
     7561 tccttattta aaatatcaaa aaactgctcc aacatattaa agatcccctt ctagatatgt
     7621 atacaaacgt tcagttctgt tttctatatc agaaattgca ttatcgaaaa taaatgcatt
     7681 tacaccatat ttattccctt taacctccct gataatacca tcattatcaa tagtaaaacc
     7741 attcacttcc gatggtttaa ttacatctga ttttttatat ccaaactcat ctaattgtgc
     7801 tctatctttc aactgtgaag ccattatccg attgttgatt tctttaacaa tataatcgct
     7861 atgagttgtt attataattt taactccata attagaaagc cttacaagca actcagccat
     7921 tttaatctga ttatgaggat gcaaatttag ctctggttca tcgattatca aataagagtc
     7981 attagacaag tagtatctaa tataaatatc cagtaaaaac aaagctttat tagatgcaga
     8041 tgacatagac agtggcattg atactttatc gccattgccg caagacgcag taaaaattgt
     8101 atcaccatca gatggagaat attgaccacc agtaagtgac tcaagagttc ttataatatc
     8161 tgcaagctca gcagactttc tgggtgacct tcgaccacga ttcgctgtcg ttgtcatgaa
     8221 cgctgttttt tcatttaaac cagaaaaatt ccttattata tttatattat gattaattgg
     8281 ttcagaaaat ttagccacct tcttattcat aatatcgtca aaagcccaca caaaatcact
     8341 tgaagataac ccagaaagcc tattccagat gtaagaatca actatattac tcctgttccc
     8401 atcaatttcc ttttgaaata gagaaatacc agttcgctca gatgtaatta taaatgtgcc
     8461 attcgcagca gaatcaagga tttcacgaac aaaaaagaag ttaaacaaaa tatttaaatg
     8521 atctgaagca tccttttctt ttaagttgac aacatactta cctgcctcat tattattttt
     8581 agatgacgca actgaaaaaa ttatacttgt gctatttttc tcttttttta tatttaatat
     8641 tatgttttca ctaatagata cgtttatatt aacctctgca ttgaccactc tgtccttgat
     8701 atgagaatca ataacacgaa tcttagagtt cttgaacatc tctgagctat ctttgaaaaa
     8761 agctggcaac tttgatgaaa gctcatcatt aactgactta atgaagccgt cagtaaaaac
     8821 attggcaata tcgtatttag ccactccatt taaaaccaaa tcactagaca ctgagtctgg
     8881 tattttaatt tttcctttta aaatagcatt atacttacta atagcagcat aggtactata
     8941 gcttaaatat gttttaccaa cattatttgg tccaaagata atagtaatat tattagtttc
     9001 tatcttcccg ctttttataa acccaatgtt ttcaaattct atttccattc atatcaccca
     9061 ctaaaatgtt atgatttgac cctggttata ttcaggttaa ctatacctat catgacaata
     9121 cactcataca tgatagttta gaaatcaccc tcaaatgagt ataaatcgca ttaaatttac
     9181 cataacgtcg cattgtacgc atcatccaaa ccgcattaag gatgatcacg acaacagacc
     9241 aggtaaaatt acctgaacgt ccagtgacgt tcataagaca ccaccgttgt tgtttaactg
     9301 attgaattat accaagccag atctgcggca accaacataa ccggacgaaa tcacctgaat
     9361 gtgtaacggc gcgacgataa acaaaaaatg cagttttaag gctgttatat agccgtgtaa
     9421 aggcttcctg ataaatacaa tgtagccagg caaacgtgtg gttatgttct gccgagatgg
     9481 atcggcaatt ctggaggtgt ccatgttact ggtcattgaa ctaaatggag ctgagttagt
     9541 gtacgatagg cttgcctatc gcttactgtt cagcagtaaa tatatcgcca tagtctgggc
     9601 gtactaatca gacggggact gggcattgcc ctttcccttt tcgcgcactg aacagaatta
     9661 cctgaatgtc caagtggggg gattaaacaa ggtgcctaaa aaggccgctg gcgcggcctt
     9721 gatgacactt tatggcgttg tcggcggagt ccatccggca cgttttgctg ccaccatcca
     9781 ttgaggggtt cctgtcgtga catgttgctg acagtaactg tatagctgct tgtataccgt
     9841 tgtcgttgtc tggttcaacc gtactttctg actggcttga ctcagtttaa tggctgtatg
     9901 ttggtcttgc atcagggatg aaattgccac ccgtagacat gatgctagtt gcggatcgac
     9961 agggggtaaa tccagaatgg cttgtagctt agtgatatgt tctttaagtg tccttgcgcc
    10021 atccatagac gctattacgt cagcgtacat tgtatttttt ccttattcac cagtgggata
    10081 gcctgatgat atcaggtcag atcacagcca gccagtagag ctggacagaa aaacctgaac
    10141 gtccaatatg gggaaaggat tacgtgacgg tgtcaacgcc acgatgtttg accgttattt
    10201 gccattttca tgcccgctaa atactttcgt tactgatgct gtccgctttt caccatttca
    10261 tcatcactga ttggcgttgt gtttttggtt agcactccag ctttacgttt ctcttttaac
    10321 cgataactct ctcctttgat attcagcgtg gttgagtgat gtagcaaccg atccagtatc
    10381 gcggttgcca gcacgtgatc tccgaacatt tctccccagt ctgcgaaccc tttgtttgac
    10441 gtcagtatga tgctcgcttt ttcatatcga cggttcagta gccggaagaa caggctggct
    10501 tcctctctgt tcatcggcag atagcctatt tcatccagga tcaacacccg ggcataactc
    10561 agttgctgta gctgacgctc cagccggttt tcctgtttcg ctttcatcag tgtcgcgatc
    10621 tgtctgtcca gtggcataaa cagtacccga tgtcccgcat ccaccgcttt cacgccagga
    10681 gctatggcca gatgagtttt tcctacacca ggaggtccca gcaggatcac gttttcgctg
    10741 cgctccacga acgccaaacc agccagttcc cgaacaacct tacggtcgat gcccggctgg
    10801 aaggtaaagt cgaactgctc cagcgttttg acccacggca gacgagcctg cttcagcctg
    10861 gactccatac cgcgctgatg cctgccgttc cattcctgct gtagcgccat gcacaggaac
    10921 tcccggtagt tcagctcttt tttagctgcc tgttccagca ggcttatcgc agacattaac
    10981 ctctgcaata gaatatctat tgcgtgattt attataaatg ttatcccttt attgttttcc
    11041 aattatcccc gcagttgaag aaatcagcac tgaataaaat tttattcatg atgcttacaa
    11101 ataaattaat gtagatgaat catcgggata gagtattcta catcaactgc attcttaatt
    11161 ttacgtcaaa atcaagcgtt gcactaaaat aaatccttac tagaatagct atccagaaga
    11221 atcaccatgt attcagtggc tgtcttcagt ctgaccctcg ggcgcagatt cttgttgtgt
    11281 taaaatatta ggaaaataaa tcaggagtga aaatgcagtg acagagacac gttacacaac
    11341 gtctgaaagc aaaaaaaagc cctgaataat cagagcttta ttactggatt ggaaatgacg
    11401 gccatcaaat ccaattcaaa acacaccaaa ggcatgtcct caagtgatga agataataga
    11461 aaactattca atctacgtca accgaatgcc tgcggtaaca atcccaaagc cgttatcgga
    11521 ggtacgatta tgttgccatt gctcaacacg ttcatgaatg cccctcttct gagtcagggg
    11581 gctactatcc tgtttcttgg gcttcttatc cttgcgctca agaaacagtg atcgcactga
    11641 gctggcttcg gccagcttct ttacttggat agaatcagct gaatgtccag gtggcgcgtg
    11701 gattatgtaa tctgatcggc gtgaagatta aagagaatag gtggttggga ttttgagcat
    11761 acgtccaatc tcggccatgt gctcatatgt actttccttg cttttacctt catgtattga
    11821 tgctatcaaa ttcaattttt gcgccgaact aataatttca ggcgttgatg gaacaaagcg
    11881 gaatccaata tggaattttt tgatatagaa aggcaaagct gaatgcttcg acattaactg
    11941 agctgcggca tctttcaact catggatgat ctcagacatt tcaacagcac aaggattagc
    12001 taatttagct tggttagcta ataacagatt tgatattttt cccaattgtt tcttaaattc
    12061 tatataaggt tcaatcgcgc tctttacaat gagctgacca ccaacaaaaa caagaacacc
    12121 agttataacg gttacagcag cggacgaact cataacttca ccaggaaatt taaccataga
    12181 agctatccta ccctgagtgt aacagtctgt ctactggaca gaaaatgcta tacgtccagt
    12241 ctgtgctgga attatgataa atgcttactt tagagatgcg actggcaaca gcttaaggct
    12301 gtcatgcgat taaggatgtg tatggaactg gatactgaat ttcgttaccc caatgttata
    12361 gatttcttcg gtacaaccct gtttcacctt tgtcatttca caaccataga tctccataat
    12421 tacgctgcat ataataaata tgctgagaat aacattgcga aaagtaataa aagaatttgt
    12481 gaagaaattg aacagcgaat aaaggaaagt gataagaaat accacgagga tattatcgat
    12541 agctaccatc aggagatgca ggaatttggt gatatttatc cccctatgca tcgaaagtca
    12601 atggttatca ctttatataa cttttgcgaa catcagatta aaacgctgtg cctagaaata
    12661 aacaaacttc ttccggaaga catgtgtgtt aaatataaat taaaagatga tatatgtatt
    12721 aaaaagtata gaaaatttct gcggcgcgaa gctggttttg acatacatcc aagtgcagaa
    12781 ttatggaacc tatgggaaaa tatgctgaaa gtggaacaaa tcaggcatgt tctggttcac
    12841 tccgaaggtg agatagagaa acacagagcg gagcggctgg ctgatataaa aagctattgt
    12901 aataagaaag aaaatatcag actgaacaaa cacaaaatta tcattgatga gggctatgtg
    12961 gcagggctta tcaccgaact tatctcactg tttgagctac tggacaggca ggtgaatctc
    13021 ttcatccgta gatacgaaag caagcatggt agctacgaag ttccacttcc gcaggaggca
    13081 tcacgaacag ccctatgatt tgcgggatag gaagccaaaa acgctcgtaa ttaattgttc
    13141 ttattgagag atacatatgg atcgcatcga atatttgaaa tggcttatgg atgaatcacc
    13201 ttctacgact cagcaactta tggcatggct ccaaagagca aaatgctata cccctgaaat
    13261 gaaagaacac cgtgatggag tacagatcga agagaatggt attatcgtag gtctgcggca
    13321 aagaacgaat ctgtataatg gggactgcct gactattcat gttgttcaac ttcctgaaaa
    13381 aatacagaat aaaggctggt ttaaatcttt tcttaaatta tgctgcgaat ctaatccgtg
    13441 gaacgatgta gttattgagg atgtaaaaaa tccatacctt ctggcatttt gtcaaaagca
    13501 taaatttaaa gtcctagctg atttttatcc tgacacctat atagttaatt cagaagaaat
    13561 tatggcgttg gagatacctc cacttaaacg gtacgaagat tatctttagt gaatagtgga
    13621 cagagttacc tgaatgtcca agtgaggtgg agaattaaac gaggacgtaa aaaaggccgc
    13681 ttacgcgacc tcagcctttt gcggttttgg cttggtgata taccacgata gacaaccgac
    13741 aataaaactc atgaacattc cagtaaaacg atcatcaatg cctggaaaca ggcaaccgac
    13801 aaacatcgca acagggaaag gaagaagtaa acagcgcccc aaaatgctca taaaatccat
    13861 aaatcctcct ttgttaaagg gcaggaagcg gttcacttcc tgcccttagt tacgtttccg
    13921 ctgtcatcag tcgatgaact ggcgaatcag ttccagaatt gcaatcagcg cgtttgcaac
    13981 ggcgaccaca gccctgagaa cgatgattaa caaattaatc atccgtttct ccagtaaaac
    14041 tggaggaaat caccgttgct tactcataca ctgcctgtca caacggctct ctcgcattgt
    14101 taggtgtaga gtgacctcct cctgccagag caccgaatac agcaccacct gtttatccag
    14161 ccaggacttt aaaatcgttc atacccgcag atacgggaag ggcaggattg caagtcaact
    14221 acatgactaa ctgactgatt atacgctaca caaaacaccc gttacaccac taaatgtggt
    14281 tccttaacca catttagttg ctttacagca ccatagtgaa taaaatgtaa acgttaggtg
    14341 tagagtaacc ttctccagtc agagccacca actcagggct ggaaaacaca aaaaagcccg
    14401 attaccagtc gggttttttt tgtgcctgtc gttcggctgt acttccgttt tttccccttc
    14461 atttgaggga tttttctttc attttttgtt gcggccaata ctgctgatcg cgttggccaa
    14521 cagaacgatg ccgttaccgg cattatcatg aatcccgttt tttcagtaag ttgcagcata
    14581 caaagtaatt aaaaaaacgt catttcttgt tgatgtatgc agtagcggtg gtgcgctacg
    14641 tgaaatctga gattttgtca aaggaattac acacgagatt tcacaaagaa gaggtttatc
    14701 gcgtagcttt aacattgtta ggacgctttt ttagcctgtt ttgaacagaa ctatctatag
    14761 taataggacg cactcccacg ctatatgccg tttaactcca aggaatggca atatgataaa
    14821 atacgctttc tcataaatga ggacttctaa tgacgattac ggcctactcc aaaacgttca
    14881 agcgtgaact tgatgccagg cagttgaaaa atctttttga tgaaaataat gccgattcag
    14941 cgctgagttt ccgggacttt gtaaaagcag atattgaatg cccagcctgc aatgtttctg
    15001 gtggccatta tgttgtagaa gctaaatcgc agtcgaaagg caatgttgta aagcaggctc
    15061 attttgcatt tcgcgacagc atgggaaaag atactcatct tcctttctgt gatttttaca
    15121 ctggttcaga caagctaaat gtcgttgcta acgacgggcg tgttgatttc atgcgttctg
    15181 gctctgaagc cacgcaactg aaccgccccg gaaatcctgg agactaaact ccctgagaaa
    15241 gaggtaaaca ggatgactaa aaatactcgt ttttcccccg aagtccgtca gcgggcgatt
    15301 cgtatggttc tggaaagtca ggatgaatat gactcacagt gggcggcaat ttgttccatt
    15361 gccccaaaga ttggctgtac gccggagact ctgcgtgtct gggttcgcca gcatgagcgg
    15421 gataccgggg gcggtgatgg tgggctcacc agcgctgaac gtcagcgtct gaaagagctg
    15481 gaacgtgaaa atcgtgaact gcgccgcagt aacgatatcc ttcgccaggc ttccgcttat
    15541 tttgcgaagg cggagttcga ccgcctctgg aaaaaatgat gccactgctg gataagctgc
    15601 gtgagcagta cggggtcgga ccggtatgta gcgaactgca tattgccccg tcaacgtatt
    15661 accattgtca gcaacagcga catcatccgg ataaacgcag tgcccgtgcg cagcacgatg
    15721 actggctgaa gaaagagata cagcgcgtat acgatgaaaa tcaccaggta tacggtgtgc
    15781 gtaaagtctg gcgtcagttg ttacgggaag gtatcagagt ggccagatgc actgtggcac
    15841 gtctcatggc agttatggga cttgccggtg ttctccgggg taaaaaggtc cgcactaccg
    15901 tcagccggaa agccgttgcc gcaggcgacc gcgtaaaccg tcagttcgtg gcagaacgac
    15961 ctgaccagct gtgggtggct gattttactt acgtcagcac atggcagggc ttcgtctatg
    16021 tggcgtttat cattgatgtg tttgccggat acatcgtggg gtggcgggtc tcatcgtcta
    16081 tggaaacgac attcgtgctg gatgcgctgg agcaggcgtt gtgggcccgt cgtccgtctg
    16141 gcaccatcca tcacagcgat aaaggctctc agtatgtgtc actggcctat acggagcgac
    16201 taaaagaagc cggattactg gcatcaacag ggagtacagg cgactcgtat gacaacgcga
    16261 tggctgagag catcaatggt ctttacaaag cggaggtaat acaccgtaag agctggaaaa
    16321 accgtgcaga agtggaactg gccacactaa cgtgggtgga ctggtataac aatcgacgat
    16381 tgctgggaag gctgggccat actcctccgg cagaagcaga aaaagcttat tatgcttcca
    16441 tcggaaacga tgatctggca gcctgagttc acagataaaa cactctccag gaaacccggg
    16501 gcggttcata cggaattaac tcttcaacag aagactggtc tttcagcttc ataaagttat
    16561 ccacttttga aatatcaact tcgttgtact ggctccccgc gtaatggact gcgctgaatc
    16621 gcttgtttga taaaggatgc cctttgacaa gagttctcag atacagaagg tcaaaaaagc
    16681 gttcatcaaa ctgtgttaca cgcctgatgg cataccagcc aggaagaatg agggcataca
    16741 tagcctcatg aatccagaca ccaagcagaa cacaaataat gcacattcca agaaaggggt
    16801 agagcggaac gcccagcccc cttattaaag cagggcgcgt aagaccttta aaaagagtac
    16861 tcatagtgcg cccatccttt aattacgcaa gccagtcagg aatatatggt gcgacaataa
    16921 agatgataat tccgataaca agtgggacta tccatgaggt atgttgacga gaaaatatcg
    16981 caatataccc aataacaaga gcaataattg taattgcagg cttacgaata tccgttaaaa
    17041 aatcaattat tttctgtaaa aaaccagttg tcttcgtaga cacatcatcc gcaaaagcag
    17101 gttctgcaac acaaagcatc aacgcagcca taaatagaga aagcgttaaa taacgtttat
    17161 tcagatttaa tttcaacatc ttttgtttcc ttttcatttg atacagtgcc acgcatggcg
    17221 tattgaggat atataatgat ttcttcaggt gtgatagtcg tcttattctc ctttttaatc
    17281 atttccttta cagaaggaac aataaaactt tttttattat caggagggct aaatcctata
    17341 cgttgtacat aacttgtatt attaaattca ggttctggct ttactcctgt ttctggattg
    17401 ccggaataat agcaactcag cccacgcact aagtcgccac ctcgtttata acagtcaacc
    17461 agtatttttt ctgatacctt aagattttcg catggatcaa acattttctc tgctgttgta
    17521 ccaaactttg caaaattggt actcgttatt tgcataagtc ctacagagta acgatgattc
    17581 cgtaattcaa tgtttttaac gatcttaagt gctgcctcct ttgattcagg aaagtaagat
    17641 actacgcctt tattaccagg tttgcgttta acctttggta ttatttccgc aatcgcatat
    17701 gggttaaaac ctgattcaac cctggcgact tcgtgcgttg tgtcgggatg aacgctggca
    17761 gcgcattgca tggcaagcgc aagaaaagta gaagtagata acataaatat tccacattca
    17821 gaacaccggg cgctatcact cgtcaggaat atcagcccca tgaataagct ctttcatcgc
    17881 ctgagatttg tattttttaa taagatactg aacaaaacca gaatcagaga tacgcctccc
    17941 cctcttgaaa cttatttcaa ttgcaatttt ttcaacttca acacgaactt ctgtaggaag
    18001 gtagagtaca ttgcgttgcg gtttccgctt tccttttata gctgtcattt tttaacctgt
    18061 cgtatttttt gttacacaca tagtcggcaa agacttgtcc acgctttgaa gcttctgcaa
    18121 ggagaagtat ttttgccaga caggagttat tcatcaggct taaattcatc gaaaaaatct
    18181 tctcctgctc ttttacttca tttatgacac ttagaggaac ttcagcagga tattcaccaa
    18241 aactgaccag tccataaaca tttttcatcc gggtaaagcg ggatggatgt acaacctcaa
    18301 aatccagaag aacgaataca taaccgggaa acattggcat gatttgcccc ctgtatgcac
    18361 tacagtcacg cctcttatag aaagtgcgta taactggatt gtagtaagcg ataccgctcc
    18421 gggataactg caacagaacc gactgatgaa aatgcggaga acactgaagc aggtaaaact
    18481 gtttctgtcc tttcatactc aatccccgat atacagatgt gacaaaacac aagccaaccg
    18541 tggttgcttg tgagaaatca cgcccctatg aagagagact ttttactatt gcgcgaagtt
    18601 tatactacaa gtggcttatg ctaagttcaa cacataaaac agatctttcg atcttgataa
    18661 atcaatttta ctttcattta ttccacataa acaacgaaac acagaatatt catcttttgc
    18721 agatctgttt tttagatcga tagatcgaat gatagctaaa acgcagaatt tcgatctgtt
    18781 tttaaggtga aaagatgagc aagcgaacta aaataaatat ttttctcgca taatcaatac
    18841 attaaatctt ataagcccca aaatttaatc cctgttttca gtttctgttc tgactgtaac
    18901 gtggatgctt tctgctgcgt aaactgcgcc attcgctgct gttgcttcag cagcatatca
    18961 gggctattca gtaccagctc tttgctggcc gaaggtgttc tgtctacgcc cttaaccccc
    19021 agattttcta actgccactc atttctgtga accgttttcc agccatgctg aacaccgttt
    19081 ttatcgagcg ccgggatttt tacttctttc tgaccaagtt tcttaagtct gacaagatca
    19141 cctgctttaa cgttttcccg cgaacataag tccccaaaat cagcccccca gtatgttttc
    19201 tcaaccccct tgttaagagt ctttagcttt ataaaatgtt gcttagactt tgttttatcg
    19261 ttctgataat ggtcgtagcc aacatcaaca acctcataaa cacctttctg tctttttggt
    19321 gctgtattat gtgcatcttt aacagactga ttaagtccat gctgttgctt atgggtcgct
    19381 ttaacgtcat aaccttttaa cttcaactct tcacaaaaac ctgtacgaat ttcccgtaaa
    19441 tcttttttcc tgatgtcagc gcgtttaccg tcattatctc tgatacgaaa aacaacatga
    19501 acgtgaggat gttctttctt gtcacagtgg tatccaagaa caaaacggtg attaggatat
    19561 tttttctgca tcgttttcct gacagactcc agcaaatctt caggctttac tttcgctgaa
    19621 actggaggcg agaagacaat attctgtgta atttttttat tttctttacc tttatcattc
    19681 atcacatgct gaggatcatt agcacgatct atcatgtgct ctttagcctc cagaatttcg
    19741 tcacccgtcc atacccgacc gctttcactc atcactggta gctctgactc acggctcata
    19801 taatcaatac tgttccgaat cccctgacga gtaacagcac ctccagatat tttgacaaca
    19861 acctccttac tggcaccctt gcggttgata cgttcctgaa cattgcggct atagttcttt
    19921 ccaccatttt tgactttatg agcgaaagct gacttacgac cattttctga tgactttcgt
    19981 ttaacacgat attctttatc aacgtaaacg cccataatca catctccact ttatttgaaa
    20041 cagttctccc tttacataac ataatgtaat tctcaaactg attcttaaga ttaaaaattt
    20101 cttttgtaag acgctccaca tcctgataga aaccatcttt atcaagaacc ctggtctgat
    20161 cattaacaat gatataatgg aaattacgac cgatcttatc aattgaatta cggcaacgat
    20221 tcatcatcag cagttcctgg tcaaagaaat ccagttcatt ttcaagtgtc gtctgtatgc
    20281 ggtaacgaca ttctcgtgat aacgtccatc cgtgacgatt agcagctctt tcaatatcag
    20341 ccctttgttt acctgttaag tacatcgttg ttttgataaa atcattatta tcaaaaaacg
    20401 tatttatatc cttactgcca gctttacttt ttaataaagc atttgctaaa agtttcatgc
    20461 ctttagctac tgttaaagct ggttcatctg gataaagagt tttaaggcaa tccagcaaat
    20521 catcatgctg attttcatca attctgaatt gaactttttt cataaaaatc tccgacaaga
    20581 accgactgta ggtcaccggg caaatgttgc ggaatggcgt cagagacgtc attttgcggc
    20641 gtttgcccta tcctgcatcg cagtgaatct ggcaccgctc ataaattgtg cttgagcatc
    20701 aacaaaaatg caaaaaggct gatgttgtca tggctgaaaa tacgtacgtc tgcgaccgga
    20761 aacccgatca atgcggtaag gacagtgcca tcaggttcgc tccgcgaacg gtctggcagg
    20821 aagcgcaagc gcagtcctgc gcaatatatc agaaagcacc tcccgaaata cgggagggct
    20881 tcggctattc agctaaggtt gctctgttca ggttgctgag tgtagccttc catgccctga
    20941 cgggcatcaa aaagccagcg ccggaagtta ctcattcttt caaggttagc tttgcttggt
    21001 tttttgccac gattaccact taatatccgc catactcgta ttcctgcttt attagtccag
    21061 tgcagaacaa tttctccttt gctgttaaaa gcaagagaaa agcgtacagc gccttgccgc
    21121 aattctccac tgacaaaaga cacaaaatca tgttttgtca taaagcctcc ggtttattta
    21181 tctgttctct tttgtgtctt tttttccagt gcttttctga gtaatgccat atactcagga
    21241 aagacatgga atggcatcaa aggaaaaacc ggacgcccac ccttttcaac gtcatttatt
    21301 gctgataata tgcgttccga cattttattc ctcgcaacag aatcaagtgt cacggtatca
    21361 ggttgcccca caacctgata ccatctttat ctttacttta ttaacttctc aattgcttct
    21421 tttttggctt tctcctgttc tgatggtggg tatgcaaggt cataccagtt atcaatacaa
    21481 cgaataactc tttctttaga taacccacat tgttcaaacc aaaattttat atttcggttt
    21541 aatgatgctt ttgcgctaaa ctctctttcc attttcagac ctttactaaa agggggaata
    21601 tccgtaagcg gctcgccaga accgtattga tatttactga gaggtcagat caactttcca
    21661 gggcaacaga tcgcgtaccc ggtttgccgg ccaatcctgg atatgctcaa tgacgtaacg
    21721 cagccatttt tctggttcca cattgttcag acggcatgtg ccgatcagcg agtacaacac
    21781 cgccgcatgt tcgccaccgc tgtcggaacc cgcgaagagc cagtttttcc ggcctacggc
    21841 cactccccgt aaggcgttct ctgcgatgtt gttgtcgatt tccacccagc cattactgca
    21901 gtacacgttc agggcctccc actgtttcag caggtatgcg aacgcttttg ccgtatccga
    21961 gtgacgcgac agtgttttca tctgttcctg tatccagtca tacagtgact gcatcagtgg
    22021 agccgctctg gcttttcttg ctgccagacg ctgttctgct gaacatcccc ggacttctgc
    22081 ctcgatgaca tacagttcac cgatacgctg cagggcttcc gtggtgatgt cggtgggcac
    22141 tcttgcatgc acatcgtgga tttttctccg ggcatgggcc atacacgcgg cttccgttat
    22201 tctgccggat tcgtataacg cccggtaacc accgtaagca tctgcctgaa gcacaccgct
    22261 gtaaccggcc aggtgattct gtggatggat acctttccgg tccgggctgt acgcgaacca
    22321 gaccgccggg ggcatctgtg aaccggcgtt acggtcatca cggacataga cccacagccg
    22381 ggcagttctt gttttaccac tgcccggctc ctggaccgga acggggatat catccgcatg
    22441 gactttaccg ggcatcagca catactggcg caggacgtca tacagcggct ccagcagttc
    22501 agcaacggca cctgtccagc gccccagcgt ggcgcggctc agctccacgc cctggcgacg
    22561 gtatatttct gactggcggt ataacggcag atggtctgca tatttcccgg taacaacatg
    22621 ggccagaagc cccgctccgg cataactgcg tgcaatgggt tttgaaggta ctgttgcctg
    22681 cacgatatgg tcgcaccgac aacaggccag tttcggacgt tgtgtttcga taaccttaaa
    22741 ggcgctgctg ataagctcca gttgctctga cacatcacac cccagaggac tgaggtcacc
    22801 accacaggcc gggcagcatt cctcttccgg ccggatgacc cgggtttcac ggggaagtga
    22861 ggccggtaac ggtttacggg ctgaagactg gcgcagggcg gatggcagta ccgggtcata
    22921 ttgctcaccc agcgtttccg ccatttcttc ctgaagtgcg ctgattcgct cctgtgcttc
    22981 ctgtatctgc cgttcggttt tagcgcgaag tttttctgag cttttaccga actgcatgcg
    23041 ctgcagtttc gcaaccagcg ccttcagccg gttgatttcg gaagcataag ccgccacccg
    23101 ctgtgagagc aggcggttgt atttcgccat ctggcggatg gtgtcctgtt gcgtctgcaa
    23161 cagtgcccgc aggcgggcat tctcatgagc aagtgaggtg tccatatcct cactttacaa
    23221 cgggttatat gcggatgcca gcgcgttccg ttcgtttcgg gtgcttccag tcgatgcctt
    23281 cgagaagcat ggacaactga gccggagtaa ggtgaacctt tccatcgcgg gtgaccggcc
    23341 agacgaagcg gccccgctcc aggcgtttgg tgaagaggca cagtccgtca ctgtcagccc
    23401 acagcacttt tatctggtca ccccggcgtc cgcggaagat gaacagatgc ccggagaacg
    23461 ggtcatcctt caggacgttc tgaacttttg aggccaggcc gttaaagcca tttcgcatat
    23521 cggtgatacc ggcaaccagc cagatacgcg aaccggcagg gagagatatc atcagtggct
    23581 gctccctttc atttcgcgga taagtgtctg taataacgcc ggtgtcagtt cacctttcag
    23641 cctgagagtt ccggccggca gaaccagctc gcagcacaga ctgtctggca gtgtatttat
    23701 ctgttcaggt atctgcactg gtgccgggat tttattatcc ggctccggag ttaacgtcac
    23761 gggaagtagc gatgtttcat gttcgccggg cggttgcagg ccaccgttac gccagagatg
    23821 ccgccagtta aagagcaggt tatcgttaat tccattctct cgagctattt gtgcgacaca
    23881 ggcaccgggt aaaagagttt gctgaaccag tctccattta aactccggtg aaaatcggtt
    23941 ccggcggggt ttctctgctt tcacaggctg agtcacggtc ggggctgaag ggtatcgccc
    24001 cgggaaaaga gcccgggcca gttgcccgca ggaatattca ccggaaagag gccagtcagt
    24061 gaatacctgg tgaaatcgcc tcaccagaga aaaaatggtg gttttgctga caccggtctg
    24121 cgcagaaatg cgcgggtacg acagtgactc gtcaaatctc agctgtagca cacgcaggat
    24181 caattccgga ttgtctgcca gtaccgcttt tcgtgcattc atcttaaatg tccctttact
    24241 gcgaaaatgg acattagtat cggaaacagg aaagggaggc gaaagacggt ttaaatgaga
    24301 cggttacgaa tatcccataa gcgctaactt aagggttgtg gtattacgcc tgatatgatt
    24361 taacgtgccg atgaattact ctcacgataa ctggtcagca attctggccc atattggtaa
    24421 gcccgaagaa ctggatactt cggcacgtaa tgccggggct ctaacccgcc gccgcgaaat
    24481 tcgtgatgct gcaactctgc tacgtctggg gctggcttac ggccccgggg ggatgtcatt
    24541 acgtgaagtc actgcatggg ctcagctcca tgacgttgca acattatctg acgtggctct
    24601 cctgaagcgg ctgcggaatg ccgccgactg gtttggcata cttgccgcac aaacacttgc
    24661 tgtacgcgcc gcagttacgg gttgtacaag cggaaagaga ttgcgtcttg tcgatggaac
    24721 agcaatcagt gcgcccgggg gcggcagcgc tgaatggcga ctacatatgg gatatgatcc
    24781 tcatacctgt cagttcactg attttgagct aaccgacagc agagacgctg aacggctgga
    24841 ccgatttgcg caaacggcag acgagatacg cattgctgac cggggattcg gttcgcgtcc
    24901 cgaatgtatc cgctcacttg cttttggaga agctgattat atcgtccggg ttcactggcg
    24961 aggattgcgc tggttaactg cagaaggaat gcgctttgac atgatgggtt ttctgcgcgg
    25021 gctggattgc ggtaagaacg gtgaaaccac tgtaatgata ggcaattcag gtaataaaaa
    25081 agccggagct ccctttccgg cacgtctcat tgccgtatca cttcctcccg aaaaagcatt
    25141 aatcagtaaa acccgactgc tcagcgagaa tcgtcgaaaa ggacgagtag ttcaggcgga
    25201 aacgctggaa gcagcgggcc atgtgctatt gctaacatca ttaccggaag atgaatattc
    25261 agcagagcaa gtggctgatt gttaccgtct gcgatggcaa attgaactgg cttttaagcg
    25321 gctcaaaagt ttgctgcacc tggatgcttt gcgtgcaaag gaacctgaac tcgcgaaagc
    25381 gtggatattt gctaatctac tcgccgcatt tttaattgac gacataatcc agccatcgct
    25441 ggatttcccc cccagaagtg ccggatccga aaagaagaac taactcgttg tggagaataa
    25501 caaaaatggt catctggagc ttacaggtgg ccattcgtgg gacagtatcc ctgacagcct
    25561 acaaaacgca attgaagaac gcgaggcatc gtcttaacga ggcaccgagg cgtcgcattc
    25621 ttcagatggt tcaaccctta agttagcgct tatggggaat atccccccct gattaattag
    25681 tagtaagaag aaaagcgatg tttttcttcc ggtagagttt tatacgcagt ttcaaaatct
    25741 tcgattttga ttgccagctc atcaccaaaa tcttttatct ggcgcttaag acattcacca
    25801 gtaaaatcat ttaaaacggg agttttaaag ccgaccattg aatgggtata ttctgaattg
    25861 gcgcgaatct cgcgaacggt gacggttttt ttaccgtgaa cagaaagaac ctggtagaag
    25921 gttacattgg tttgctcata gccccaggat gatacaaaga catcaccaac agagacagta
    25981 tcatttttaa aagttaatac tttcattttt agctcccgcc cagcttgggc tctggatgaa
    26041 agagaacctg tttccctttc ttgtgtttta ttatacctgc atttgcaggt atagcaagtt
    26101 atttttttac cagcctgtaa gacggatgca tatttgctaa tagcaataaa tactgatatt
    26161 caccaacaga taaagaacga ctggccttac ctgcactttc tttcatctgc caggaattca
    26221 gttgataacc aaaaatatca gcagcttctt taagagtaag acctgccgca acccgtgcat
    26281 tctttacttc ttcaggtgta ggtttattct gcattttcat ccccttttgt cagacaatca
    26341 cacaaaatcc cattatccag aagcatcaat gatgccaggt tgagtttttc gtccattatc
    26401 aattttctta ctgttttcct gtttttagtt ttatcaatcg tcttaacata ttttaagacg
    26461 tgaaaaaccg catccccatc ttcatccatc gttctgatta catatgagta attacctctg
    26521 tttttcttaa ttatcttatc tttattcata ttattcctca taataactaa aaaaggggct
    26581 taatgcccct ttttatctat aacacggtat gtaaccctgt ttcttgtgta aataaacacg
    26641 ctaactgatc acaatagata ttatcgaatc ttgaaatatc tttaacttca tcaccccgta
    26701 cacgagaaaa tttcacctgg tacgtatcag taaaatccag tttgattttt actaagttga
    26761 tgcctttcat tgcaaaattg gacggcaaac ggaaagttaa cccgcactca ccgttttcat
    26821 caaagtaaga aaaactttta gccccagtca taacaataaa acgattacca ccaagttgac
    26881 gtaaaatctc tgtggctaca gacttagcgt agttttgcat ttttaacctc ccgcccactt
    26941 tgggctctgg atgaaagaga acctgtttcc ctttcttgtg ttttattata cctgcatttg
    27001 caggtgttgc aaatttttaa tgccatgttc tgactgtaaa cagtcaatca tttgatgatt
    27061 aatcttagcg caaatctgcg atctgataaa cttcgctgct ttcccttctt ccaacggcaa
    27121 taacgagtaa aacgactttc tcatcaatga cttgatatac aagtctgtag ccagatgcac
    27181 gaagtttgat tttatagcga tcagcatgac catgtaaccg ggctgcgggt acgcgtggat
    27241 tttccagccg ttctgccagt ttttttttga attgttcacg gatggtgtgg ccgagtttct
    27301 gccattcctt cagtgctctg cggtcaaaag ccagttcata ggtcatccag gttaaccttc
    27361 acaatttccg ggtttttcat gcgctcatca gcaacagcgt ttaactcagc atcttcagcg
    27421 agttcccgat agtaggcgta aagttttggt ggaacgcaat agaacgccgg ttcgtttcgg
    27481 ttcaggatcg caacagcgtc cccttcacct tcagctacgg ttcccatagg attttttttc
    27541 aggtcagtaa tactggccgc tgtggttgtc agaatttgat agctcataag tcctccgctt
    27601 gtctcattgg cataatagca tctttaaaag cactcttaaa agtgcttttg ttgagaaaaa
    27661 catcagaaca ggtgagatat ggctctttcc gacatcgcct taaaacgctc tgtaacgcgt
    27721 tctgagcgtt ttttcccgtt tcgttatagt cagctacggt ttaacggttt ttaacgccgc
    27781 tcaacgcttt ttagcggcct tgttgctgtg atctgttgcc atgatccgga taggagttca
    27841 atcttgatca aaatttcctc aaggcaacga ctgttttgta aataccctct aaaatgcgct
    27901 gtatcgcatt ctgagcgatt ttttcgattt cgcatggttt tcctcatggt tacatcgaac
    27961 aaagcgttag aaagcgattc agggcttctc agtgaaagat ccaaaaagga tcaataaacg
    28021 gatcgtatga tctgaactct tctggttttg agaaaggaga caccgctcgc cgcagtcgaa
    28081 cgaccgagcg tagcgagtta gtgagcgagg aagcggaaca gcacagggat aacattaggc
    28141 aattacgcgc agaggttaaa aagcgagcat attgttttta tgtgtggttg tcctggttca
    28201 gagtgatctg tcctccgggt ttacgcaaag ttatccacag gctctgtgca tttaaaaaac
    28261 ttatcttttc ttcttgatct taaaaactta aaaacttaga gagccgtaac atcatgaaaa
    28321 acataataaa aaaacgctaa agttgagagg atttttaaca taagttgaga agatttttaa
    28381 gtaaggttga gaagattttt aactaaagat gagaggattt ttaagtaagg ttgagaagat
    28441 ttttaactaa agatgagagg atttttaagt gtatccacag atttaaaaaa gatcatgaaa
    28501 aacataatgt tatgaattca ttttgatgtg aatacataaa gatctttata tctaaaggtg
    28561 ataagctttt taagaaaagg ttgtagtttg tagttgatat acaacctttc aggggttatc
    28621 atttagtcaa ttgatatggc ttattctata t
//
