LOCUS       CP057401                6888 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB27-C22 plasmid pRHB27-C22_6, complete
            sequence.
ACCESSION   CP057401
VERSION     CP057401.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148265
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH04 T2-C22
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 128.21224652643x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:39:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,597
            CDSs (total)                      :: 5,478
            Genes (coding)                    :: 5,270
            CDSs (with protein)               :: 5,270
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 208
            CDSs (without protein)            :: 208
            Pseudo Genes (ambiguous residues) :: 0 of 208
            Pseudo Genes (frameshifted)       :: 72 of 208
            Pseudo Genes (incomplete)         :: 145 of 208
            Pseudo Genes (internal stop)      :: 37 of 208
            Pseudo Genes (multiple problems)  :: 39 of 208
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB27-C22"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB27-C22_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6702..6888,1..233)
                     /gene="exc2"
                     /locus_tag="HVY48_27940"
     CDS             join(6702..6888,1..233)
                     /gene="exc2"
                     /locus_tag="HVY48_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMG87846.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(796..1029)
                     /gene="mbeD"
                     /locus_tag="HVY48_27945"
     CDS             complement(796..1029)
                     /gene="mbeD"
                     /locus_tag="HVY48_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMG87847.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(1036..1554)
                     /locus_tag="HVY48_27950"
     CDS             complement(1036..1554)
                     /locus_tag="HVY48_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMG87848.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            complement(1532..2074)
                     /locus_tag="HVY48_27955"
                     /pseudo=""
     CDS             complement(1532..2074)
                     /locus_tag="HVY48_27955"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2232..2555)
                     /locus_tag="HVY48_27960"
     CDS             complement(2232..2555)
                     /locus_tag="HVY48_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMG87854.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2620..2811
                     /locus_tag="HVY48_27965"
     CDS             2620..2811
                     /locus_tag="HVY48_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMG87849.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3836..3973)
                     /locus_tag="HVY48_27970"
     CDS             complement(3836..3973)
                     /locus_tag="HVY48_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMG87850.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            4021..4362
                     /locus_tag="HVY48_27975"
     CDS             4021..4362
                     /locus_tag="HVY48_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="QMG87851.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin transporter"
     gene            complement(4359..5924)
                     /locus_tag="HVY48_27980"
     CDS             complement(4359..5924)
                     /locus_tag="HVY48_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /protein_id="QMG87852.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-10"
     gene            6184..6621
                     /locus_tag="HVY48_27985"
     CDS             6184..6621
                     /locus_tag="HVY48_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMG87853.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="entry exclusion protein 1"
     Typing          complement(2897..2984)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          2995..3553
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.606"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(693..2242)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.031"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcggcagtt ctgggatatg ggagctaaag ataaagcgac aggaaaatca cggagtgatg
       61 tgcaacaacg cattcaacag tttcacagcc aggaattttt aaactcactc cggggcacaa
      121 ctcaatttgc gggtactgat taccgcagca aagaccttac cccgaaaaaa tccaggctgc
      181 tggctgacac gatttctgcg gtttatctcg atggctacga gggcagacag taagtggatt
      241 taccataatc ccttaattgt acgcaccgcc gaaacgcgtt cggtgcgatc acggcagcag
      301 acaggtaaaa atggcaacaa accacccgga aaactgccgc gatcgcaccc ggtaaatttt
      361 aaccacatgc atacctatgc agccaggtga atcacgcagg aattgccagt cagagaccgc
      421 tgaaacggat ttttttcata ctttcggaag cgaagaagac ggggatttga gcaggaaaac
      481 agaagttcag gggaatgagc gcgatctggc aatagaggca aaaacacgac tatgaaagac
      541 acaccagaat cgcgcccgta tgcgttttaa cgcgttcctg tgttttcagc aaggtttatg
      601 actgaagcgc gttcccggtg ccccgaacca accattatcc caaaaaatgc accggaaacg
      661 cgctgacaac attttagcct gtctctgatt accatcccag cgaagggcca tccagtgtcc
      721 gttcctgtat ttccagctgg cgctcacgct ccagggataa ctcatgttcg cgttcctgta
      781 tcagccgttc gtcccttatc tcctgttctc gctgtataac tggctcaagc gttctgtccg
      841 ctcgctcaag tgctgcacct gctgactcaa cttcatgacc cgctcgttca gcatcgcgtt
      901 gtcccgttgc gtaagcgaaa acattttctg caattccacg aaggcgctct cccattcgtt
      961 cagccgctgc atatagtcct gttgcagttg ctctaatgcg ttcagcaaat gtctttccag
     1021 ctccgtcact ctgtgtcact ccatcagttg tacccagtcc ttcccctgat ggggaatcac
     1081 ctctgctgat ttcccgtacg gaaggaccag aaatttcctg ttcccgtcct gtacgaactc
     1141 cacgccccat gtcttcgcgt tcagtttctg caatgtctct tcctgcttcc tgatatcttc
     1201 caggttcgcc tgtatcatcc ctccaagata ccagagcgtc ccgcaactca cggtaaacag
     1261 gaaaatgacc atccccagta acatcatgcc cgtattccct gccagcttta acacgtcctt
     1321 cctgtgctgc atcatcgcct ctttcacccc ttcccggtgc tttttcagtg attcctctgt
     1381 cgaagctgtg aacagggcta tagcgtcttt gattttcgtc tcgtttgatg tcacagcctt
     1441 gcttacagat ttttcgagct tgctgaactc gtcgttcagc attgtctctg tagattcggc
     1501 tctctctttc agctttttct cgaactccgc gcccgtctgt aaaagattgc tcatagaatg
     1561 ctcctttcag tctgatattc ttcccgccgt tcgggtctgc aatgctgata ctgcttcgcg
     1621 tcaccctgac cacttcaatc cctgcttccg tgagcgcctg aatcacatcc tgacggcctt
     1681 ttatctctcc tgcatggtaa agagcatcta tcccgcgtgt gacgccctca gcaaacgcct
     1741 gtttcgtttc cggcaggtta tcagggagag tcagcgtccg gcggttctcc ggggcgttcg
     1801 ggtcatgcag cccgtaatgg tgattcacca gcgtctgcca ggcatcaatt ctcggcctgt
     1861 ctgcccggtc gtagtacggc tgtaggcgtt ttccggtctc cagctccatg ttcggaatga
     1921 caaaattcag ctcaagccgt cccttgtcct ggtgctccac ccacaggatg ctgtactgat
     1981 ttttttcgag accgggcatc agtacacgct caaagctcgc catcactttt tcacgtcctc
     2041 ccggcggcag ctccttctcc gcgaacgaca gaacaccgga cgtgtatttc ttcgcaaatg
     2101 gcgtggcatc gatgagttcc cggacttctt ccggattacc ctgaagcacc gttgcccctt
     2161 cccggttacg ctcccttccc agcaggtaat caaccggacc actgccacca ccttttcccc
     2221 tggcatgaaa cttaactatc atcccgcgcc ccctgttccc tgacggccag acgcaaccgg
     2281 cgcagctcat ccccgatggc catcagtgcg gccaccacct gaacccggtc gccggaagac
     2341 cactgtccgc tgttcacctt acgggctgtc tggttcaggt tattcccgat ggctgccagc
     2401 tgacgcagta acggcggtgc cagtgtcggc agcctcccgg aacgggcaac cggctcaccc
     2461 aggcagaccc gccgcatcca taccgccagt tgtttaccct cacagcgttc cagtaaccgg
     2521 gcatgttcat catcagtaac ccgtatcgtg agcatcctct cgcgtttcat cggtatcatt
     2581 acccccatga acagaaatcc cccttacacg gaggcatcag tgactaaaca ggaaaaaacc
     2641 gcccttaaca tggcccgctt tatcagaagc cagacattaa cgctgctgga gaagctcaac
     2701 gaactggacg cggatgaaca ggcagatatc tgtgaatcgc ttcacgacca cgccgatgag
     2761 ctttaccgca gctgcctcgc gcgcttcggg gatgacagtg aaaacctctg acatatgcag
     2821 ctcccggaga cggtcacagc ttgcctgtga gcggatgccg ggagcagaca agcccgccag
     2881 ggcgcgtcag ggtgttttag cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgat
     2941 agcggagtgt atactggctt aactatgcag catcagtgcg gattgtatga aaagtgcacc
     3001 atgaccagtg tgaaataccg cacagatgcg taaggagaaa atgcacgttc cggcgctttt
     3061 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtactga
     3121 ctcactcaaa aacgataata cagttatcca cagaatccgg ggataagacc ggaaagaaca
     3181 tgtgcgcaaa aaggcaaagc ccaacaaaag gccacgccga tggcgttttt ccataggctc
     3241 cgcccccctg acgagcatca caaaaaccga cgctcaagtc agaggtggcg aaacccgaca
     3301 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3361 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3421 catagctcac gctgttggta tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt
     3481 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3541 tccaacccgg taagacacga cttagcgcca ctggcagcag ccgttggtaa cttggttagc
     3601 agagcgatga tggcgcaaat ggtgcaacag agttcttgaa gtagtggccc gactacggct
     3661 acactggaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc tcggttaagc
     3721 agttccccaa ctgacttaac cttcgatcaa accgcctccc caggcggttt tttcgtttac
     3781 aggcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact
     3841 gaacagagat ccccgtcaat ttagaagaag atgatggagc cacagtccct ccctgaacat
     3901 cacgtatata gttagcctga catccaacaa ggaggtttat agcgaatatt cccacaaaaa
     3961 atcttttcct cataactcga tccttataaa atgaaaagaa tatataacga ggtttaattt
     4021 atgagtttaa gatactacat aaaaaatatt ttgtttggcc tatactgcgc acttatatat
     4081 atatacctta taacaaaaaa caacgaaggg tattatttcc tagcgtcaga taagatgcta
     4141 tacgcaatag tgataagcac tattctatgc ccatattcaa aatatgctat tgaacacata
     4201 ttttttaagt tcataaagaa agattttttc agaaaaagaa aaaacctaaa taatgccccc
     4261 gtggcaaaat taaacctatt tatgctatac aatctacttt gtttggtcct agcaatccca
     4321 tttggattgc taggactgtt tatatcaata aagaataatt aaatacccaa cacctcattt
     4381 atagtattaa gtttattctt atcaataaag gcacatagaa tgcctgtaac aatagcaatc
     4441 ccccagatac ctaatgtagt tccagcaagc acactaaaaa gtaaaacaac aacataacta
     4501 actccagcat ctacagcttt cttctctaat gtaagaaata gcggcttcca gtcacctgta
     4561 tccttaattt ttaggatatc agataccaca tcatatccaa aagaaacatg tcccgtaatc
     4621 ttcaagtact tggcaaactg atctaaatgc ttagcccagt cttcatactt aaccgcctcc
     4681 agtgcattga aaattgcatc acggtctgct ttgctgaatt tcttatttaa tacatccttg
     4741 tatttctcaa aagcagctag agcttcattc acattgctga ttttcttacc tttagattta
     4801 tcagcaagtt cctgtgccat ttttgaatat ttttcaccat atttttcaga tagcgtttga
     4861 taaaagctaa ctgttgcatc cacagcatcc ttaatctgcg agttcaggag attagtctgt
     4921 gctgttttca aattatcttc agcatcatga atacgtgaaa ctgctgtact tcgcttatta
     4981 ttagcctgag aaatagcctc ttggattcca tttatctcag agctaatctg gttaagacgc
     5041 gtttctgatg ctgttacctg cttttgtttt tcctccatct catcaccagc tctcgcccgt
     5101 agtctggtgg cctcaaaaaa aggacgactc tgaagcggat cattcgccct aggagataaa
     5161 agtttaaccc tttcatccag ttctttatat ttagcagatg cctgatccaa ctcatttcgt
     5221 tttccggaca gcgtattcgt ttctgcatca cgggcatgaa tactggagct taaacgggta
     5281 ttgagcgtat taatctcttc atccactttc gccagctcag attgtgcagc agcaagattt
     5341 ttttgtgcca cctccacagc ccgggcctct tcactcaatg ctgccaggcg tttctcttca
     5401 tcctcagcca gtttcaattg gcgttctgtt tcagcctgct ccttctctat ctctttacgt
     5461 cgttgttctg cttcctgaaa agccttttcc gcagcttccg cttctttacg ggctttttct
     5521 tctgcttttg caaggcgcaa ccgctctgct tctgcctgca tcgctgcatt attggcatga
     5581 gccaggtcaa taacctccgg atgagtggaa ttatggcgaa gcgcctcatt cacaatatcc
     5641 ttcagatgtt gagtcagcgc atcccggttt gcttttgctt tagcctgtgc ttctgcggca
     5701 gcttttgctc gggcagcctg ttctgcctgc gttttcttca attgagcagt agaccattta
     5761 gctgtggcat gaatggctgc agaactttca cttttgctac ctccagttcc accaccaccg
     5821 ccagagccac tcccgtctgg attaccattc aaaagagtaa tgattacctg ccccttatca
     5881 tcataaggaa caccatcttt atagtacgct acagctgttt ccattataaa atcctctttg
     5941 acattaaaaa caaatagtta aaagcaatat actgtatata taatcactgg ttttatatac
     6001 agtttaaaat tacgccgctg cattttccct gtcaaccctg tggattttca tttttgtgaa
     6061 aacgatcaaa aaaacagcca cccacaccac gtgctatttc cacccgatgc cacaaaaacc
     6121 agcacaaaca ttaccgttct cagacctcat tatgttttac tgaaaccatg agatgagaca
     6181 cctatgggac actgtaactt tatggcatgg cacacactcc gggacgcact gaaaatgact
     6241 ggaagatcgc gttcacagtt ttaccgtgat atgcgctcag gtcttgtcag ttaccgtacc
     6301 agccaggacg gacgacggga gtttgaaacc agtgaactga tccgggcata cggcgaatta
     6361 aagcggaatg agacaccaga aaggcacact caaggacact cagaaaaacc tcatgatcag
     6421 cagacagaac gcattctccg ggaactgaat gagctgaaac aatgcctgac gctgatgctt
     6481 gaggataaac aagcccagga tacggagcgc cggcgccagg aagcagaacg gaaacagcta
     6541 cagaatgaga tagcccagct caggcaggct ctggagcagg aaaagaaacg gggattctgg
     6601 tccaggctgt tcggtcactg aacgcgctca gaaactgatg ataagataat cttcggataa
     6661 taactcaccg agaataaata ctttaaggta gggagacact catgagacgt accggaaaca
     6721 aactttgtct tatcgccatg ataacagcaa cagtagctct cacagcctgt accccaaagg
     6781 gcagcgtgga acaacatacc cggcattacg tatatgcttc tgatgacggt tttgatccca
     6841 acttttccac ccaaaaagcc gacacaacac gaatgatggt gccttttt
//
