LOCUS       CP057387                8136 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB28-C05 plasmid pRHB28-C05_4, complete
            sequence.
ACCESSION   CP057387
VERSION     CP057387.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148267
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T2-C05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 195.471565422958x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 07:41:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,443
            CDSs (total)                      :: 4,324
            Genes (coding)                    :: 4,061
            CDSs (with protein)               :: 4,061
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 119 of 263
            Pseudo Genes (incomplete)         :: 122 of 263
            Pseudo Genes (internal stop)      :: 46 of 263
            Pseudo Genes (multiple problems)  :: 22 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB28-C05"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB28-C05_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(7975..8136,1..542))
                     /locus_tag="HVY50_22140"
                     /pseudo=""
     CDS             complement(join(7975..8136,1..542))
                     /locus_tag="HVY50_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            791..2245
                     /locus_tag="HVY50_22145"
     CDS             791..2245
                     /locus_tag="HVY50_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="QMG78516.1"
                     /translation="MTALVSSFKWVGIGQLVKIATQLVSLFYLTHLIPPSSYGLLAMSS
                     VVMNLASIFNDMGTGAAVIQRQEASIGFYNYIYRINIFTGLFVMLLVIITSPLIVVYFN
                     TPELYPVLFLLAFSFPISSLTITHKAKLEKEMNFKVIIAIEIFSSLTGMVLAIVLANLG
                     FGVYSLIFQTLTTISISTICFLKVSGLSLSLRIKNISHSDKSGVIGFSGYLLSFNLINY
                     FSRNLDSILIGRFFSSAILGVYSIAYRIMLFPLQSLTLVFSRVFLPHLSKNINNTNNTN
                     KQDYLNALKLILSLSAPLMLGLSATSFSFTRIFFDPKWHLLGDLLHWLAPTAIIQSALS
                     TTGSVFMAYGKTRWLFFLGCIGAFLMGLSFIIGVHFDIKTLVYLYFFANLINSLPAFIL
                     LKNILKFSYIELVNVFFRTISPAFLMFLLIKVWQHFSFFKNASIDFSFNVALGIFSYIV
                     FYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(2338..2605)
                     /locus_tag="HVY50_22150"
                     /pseudo=""
     CDS             complement(2338..2605)
                     /locus_tag="HVY50_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(2577..3042)
                     /locus_tag="HVY50_22155"
                     /pseudo=""
     CDS             complement(2577..3042)
                     /locus_tag="HVY50_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(3241..3525)
                     /locus_tag="HVY50_22160"
     CDS             complement(3241..3525)
                     /locus_tag="HVY50_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QMG78511.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4146..4463)
                     /locus_tag="HVY50_22165"
     CDS             complement(4146..4463)
                     /locus_tag="HVY50_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG78512.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="hypothetical protein"
     gene            5548..5697
                     /locus_tag="HVY50_22170"
     CDS             5548..5697
                     /locus_tag="HVY50_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG78513.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            6080..6448
                     /locus_tag="HVY50_22175"
     CDS             6080..6448
                     /locus_tag="HVY50_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG78514.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            6533..7516
                     /locus_tag="HVY50_22180"
     CDS             6533..7516
                     /locus_tag="HVY50_22180"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="QMG78515.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENQGQSVARNVAIESSTGEYIWFLDGDDKISHGSLKLIDKI
                     TNEKNYGIIFFNAHVFFDTPDLKKKGWHDYKRSTFGEIDALTFFEKEMANKKLIVSPCC
                     YVFKRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEELFLRRVRNDSVMTSKK
                     SMRHIIGYSTCIDELTKYKKIKGIQNKSFDRFVNGMVLSLFSISETIKTKEAMNYVARY
                     KKHPGLSFKDSVSINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     Typing          5340..5513
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          complement(4865..5373)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 aacgtgcagc tttcccttca ggcgtgattc atacagcggc cagccatccg tcatccatac
       61 cacgacctca aaggccgaca gcaggctcag aagacgctcc agtgtggcca gagtgcgttc
      121 accgaagacg tgcgccacaa ccgtccttcg tatcctgtca tacgcgtaaa acagccagcg
      181 ctgacgtgat ttagcaccga cgtagcccca ctgttcgtcc atttcagcgc agacaatcac
      241 atcactgccc ggttgtatgc gcgaggttac cgactgcggc ctgagttttt taagtgacgt
      301 aaaaccgtgt tgaggccaac gcccataatg cgtgcactgg cgcgacatcc gacgccattc
      361 atggccatat caatgatttt ctggtgcgta ccgggctgag aggcggtgta agtgaactgt
      421 agttgccatg ttttacggca atgagagcag agatagcgct gatgtccggc agtgcttttg
      481 ccgttacgca ccacgccttc agtagcggag caggaaggac atctgatgga aatggaagcc
      541 acgcaagcac cttaaaatta ccatcataca ctaaatcagt aagttggcag cattacccac
      601 tattggtgga gatgatcata atgccttata atcatacggg atacgaactt ataaagtcct
      661 aaagtttagc taaatcggaa tctacaacgt agatgatagc ttgaagaatt atgatgtctt
      721 ttatggtggt atctttaaaa ccatcacgga ttataaatta ttgaaaattc aaaaggatat
      781 taaaaacaat atgactgcac tagtcagtag ttttaaatgg gttggcattg gacaacttgt
      841 aaaaatagca acacaattgg tttcgttatt ctacttgacg cacttaatcc ccccatcgag
      901 ctatggacta ttagcaatgt catctgtggt tatgaattta gcaagcatat tcaatgacat
      961 gggaactggg gcggcagtaa ttcagcgaca agaggcgtcg atcggttttt ataattacat
     1021 atacaggata aacattttta ctgggttgtt tgttatgctg cttgtaataa taacatcccc
     1081 attaattgtc gtgtacttta acacacccga gctatacccg gttctatttc tattggcttt
     1141 tagctttcca atttccagct taacaataac tcacaaagca aaattagaaa aagaaatgaa
     1201 ctttaaagtc atcattgcaa ttgaaatttt ctctagcctt acaggaatgg tgctggcaat
     1261 agttttagct aatttaggct ttggtgttta tagtttgatt tttcaaacac tgacaacaat
     1321 atcaatatcc acaatatgtt ttttgaaggt ttccgggttg agtttatcat tgagaattaa
     1381 aaatatcagc catagtgaca agtcaggagt tatagggttt agtggttatt tattatcttt
     1441 taatttaata aattattttt ccaggaatct tgattctatt ttaataggcc gttttttctc
     1501 atccgcaata cttggggtct attcgattgc ctaccgcatt atgttatttc cgttgcagag
     1561 cctgacgctt gttttttcta gagttttctt accacacctt agcaaaaaca taaataacac
     1621 taataacact aataaacaag attacttaaa tgcattaaaa cttatactat cattaagcgc
     1681 gccactgatg ctaggtttat ctgctacatc tttttctttt acgaggattt tctttgatcc
     1741 taagtggcac ctacttggag acttattgca ttggttggca cctacagcga ttatccaatc
     1801 agccttgagt acaacaggtt ctgtttttat ggcctacgga aaaacaaggt ggttgttttt
     1861 cttaggttgc attggcgctt ttttgatggg gttgtctttc attataggtg ttcatttcga
     1921 tataaaaaca ttggtctacc tttatttttt tgcaaactta attaattcac tcccggcatt
     1981 tattttactt aagaatatct tgaagtttag ttacattgaa ttggtaaacg tgttttttag
     2041 aactatctcc ccagcttttt tgatgttctt acttattaaa gtatggcagc atttttcctt
     2101 tttcaaaaat gccagcattg atttttcttt taatgtcgca ttgggtattt ttagttatat
     2161 tgttttttat accttgttta atttcaatga ggttgttagg acaggagtaa ttcataagtt
     2221 aagatgcatg accaagttaa agtgattcat ttattaaatt cattaaatga attggtaatg
     2281 actccaactt actgcttgtg ttttatgttc agataatgcc cgataccgac tgcggcctaa
     2341 tttttttaag tgtcgtaaaa tcgtgttgag gacagtgccc ataatgcggg atgtggcccg
     2401 gcatccaacg ccattcatgg ccatatcaat gattttctgg tgtgtaccgg gctcagaggc
     2461 ggtgtaagtg aactgtagtt gcttgctatg ttttacgaca gtgagagcag agatagcgtt
     2521 gatgtccggt agtgctttta ccgttacgca ccacgccttc agtagctgaa caggagggat
     2581 aaccagtcca gcgtgtagag ttcaggctgc catacgggca attttccagt gactgtggtt
     2641 cggattcttg caaattagcc ccgaataccc cgtgtcagcg cccaattttt tacgtagcgc
     2701 gctctctaca gcagcggcgt aacgtaacgg tttcattttt ccgtctggcg cggtgcgtat
     2761 cgaggttttc agtgcgtaca gcaggtgcgc gtgtccgttt tcggggttgg tgatggtcag
     2821 tgtcggcgcg ggggcgttgc ggtcgctcca gtcaatggct gcgccgatgc ggtcaacgtc
     2881 aaatcccagc cagaacatgg cgtgaggctg attaaactgg atgtatttag ccagaatagc
     2941 gcgctctttc cctgcgatgc ggacaccaaa gtgtaaatca tccgagaagt acggtttatg
     3001 cggcaagcgg tcgttgaaaa gttcgagtgc tgcgttacgc aatgtgccct gaacttatgg
     3061 cgggctgttg tctggcacgg ttcatgtgct aatatccttc ttaggtttta tacctagccc
     3121 tgatttatca tgtctcttcc gccaagttga aatcatgatt atcggggctt tgttttttct
     3181 gttgccgtta aagttacacg cgaacggcct cgcctgtcga atttcttctg tcacatcagc
     3241 ttaatgcgcc gccagttcat caaaaaactt tgggttagcc tgaatggtgg ccagcacttt
     3301 tgctgccagt ccggttgggt tacggcgtcc ggtttcccag cttttgatgg tatcgaggct
     3361 ggttcccagc actttcgcca tttcgctctg agtaatattc aggttttctc tgatagccct
     3421 gacgtctgcc agctcgtagc gggtcacacg ggcgggggct ttggttccgt ttttgatttc
     3481 aacggcctct tccagagagg ctttcagctc gtcaaaaaaa ctcatcttac acctcgtctt
     3541 tcagtcgttg ggtcagcttc ttcagttctg ctttctctgc gtctgtgatg ctgtctttgg
     3601 tgcttttagg gtacgtacag catgtagaca acatgtccga aagtaagcaa aaccattaac
     3661 ggacagccgg atcatttcgt acatccctta cggtcagggg ttcgcgctca atggaacgaa
     3721 atcacccgtt agcttaaaac gcttaacgta ggataatttc ctttttccaa gcggacccgc
     3781 tctactcgaa attgtcggat acctgcaatt acctaccgtg ccttacggct tggtcaatgc
     3841 ctcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggataaga agaacatcgg
     3901 aaacaggact taccccggct gaatggcgtg aatttccgcg ctatgcactt gcgcgcatac
     3961 tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg ggttttcggg tggtttgttg
     4021 cctgttttac cggttttccg tcagaaacgc gctgagggcc tctgaggcgg tgcggccaac
     4081 ggatgttatg gtaaattcag ctgattggca ggtacaaaaa aagccccgca gggctttttt
     4141 gtaccttatt gatctgcaca ctttgcagcc ttcagggctt ccagctgatt tagccggtta
     4201 gctgcttttt tgcgggcttc gccttttgcc atgccatttc ctatcccaaa atcacccagg
     4261 aaacctaata cggtgcgccc gtcaaattcg ccggtttcct caatttcttg ttgtacactg
     4321 cgcgttttgg cgatttccag agctaccgct ttgcaatcca ttgcggaaga ttctggaccg
     4381 gtgacgttca cggcctgtgg atattgcttt gttgcgcatc ctgccatgat cagcgctgcc
     4441 gataggatga atattttttt caaaattatc cttatttatt taggcatagt taagagatac
     4501 ccattttatc ggcaggaaat gagggttttt tagttgaatt ttaggagggg caaggttcca
     4561 tattggtcga ggattctgga gctgaccggc aacggccggc gcggctatgg gccatgcggt
     4621 ggttgagcat agaaaggatc ttcttgagtt cctttttttc tacgcgtaat ctcttgctct
     4681 gaaaacgaaa aaaccacctg gggaggtggt ttttcgaagg ttaagtcagt tggggaactg
     4741 cttaacctgg taactggctt cagcagagcg cagataccaa atactgtcct tccagtgtag
     4801 ccgtagttag gccaccactt caagaactct atagcaccac ctacattaac tcgctctgct
     4861 aatcctgtta ccagtggctg ctgccagtgg cgctttgtcg tgtctttccg ggttggactc
     4921 aagacgatag ttaccggata aggcgcagca gtcggactga acggggggtt cgtgcataca
     4981 gtccagcttg gagcgaactg ccttcccgga actgagtgtc aggcgtggaa tgagacaaac
     5041 gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga
     5101 gggagccgcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
     5161 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct
     5221 ttgccgcggc cctatcgctt ccctgttaag tatttccctg gcatcttcca ggacatctcc
     5281 gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt
     5341 gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgccgcctt
     5401 ttttgtcctg ctacatgaag cacttcactg actccctcat cagtgccaac atagtaagcc
     5461 agtatacact ccgctagcgc tacgtgactg gttcagggct gcgccccgaa accctctaac
     5521 aacctctcag ctactggata tcaccccatg ccatctgaac atgatgaaca actggattcg
     5581 actgatacgc agcttcccgg cgaatatgac ctttttgatt taatggcgca ggttactgaa
     5641 gagaacctgc atgccccggt ggattttggg ccgcctgtcg gccgggaagc gttatagctt
     5701 tttcgcttac ccgcgtacgt ggtaatacgc cggtattaca tcttatttct ttattttcat
     5761 gtaattacag gtggttgtaa tacgttgcgt attacataaa taaacgggga cttcagtttt
     5821 tatcttttaa ttcatgcaat tgcgatgaaa atcgcggttg cgtatggtgt atgaccattt
     5881 aagggataat gtaacctgac ttttaaagaa tgaatctatc tttggcagcg catgagatag
     5941 ataaaaagcg ttccacatca ggcggttcat gtatccatga accggaaagg acaaaaataa
     6001 gacaattgta agacattttt tgcgttttac tcttattttg gactattatg agacaaatgt
     6061 aagacattat gggcggtata tggcgaattt taaccctggt ccaagaacag ccattgcagc
     6121 tgtcagggat tccaagaacg atatagaaac ctggaagaaa aatggtattc gtagggaaga
     6181 gattgtgact gtgctgaatg aacgttacgg cctgaatatc tcactggttg cgtttgataa
     6241 ggctctttac agattgagaa aaagtacaga tgaaccgtct cttgctgatg tggattctgt
     6301 gtctgctgca gaaacgaatt tctctgaagc cagtgggcct gacaatggtt tttttctgtc
     6361 cgggaatgac ttcatgcgag cagaagaaga atctcaaaga gtggttgagt cattcagaga
     6421 tagccttagc aggaagaaag gattctgatt tattcaacaa agccgttgat aaatcgcatt
     6481 ccataaataa caacttgatg ttagtataac taaatattat ttggagatta ctgtgaaaaa
     6541 aaaattttcg ataatagtac ctgtatataa cgttgaaaaa tacttaaggg aatgtattca
     6601 atcagtatta gatcaggatt ttaacgacta tgagataatc tgtgtaaatg atggttcaac
     6661 agatggcagt ttagatatca tactgtcatt tgatgaccaa agaatcaaag taataaatca
     6721 ggaaaatcaa ggccaatcgg tggcaagaaa tgtcgccatt gaatcatcaa ctggtgaata
     6781 tatatggttt ttagatggtg atgataaaat atcacatgga agtctaaagt taatagacaa
     6841 gataaccaat gaaaaaaatt atgggattat tttctttaat gcacatgttt tttttgacac
     6901 tccagatctg aaaaaaaaag gctggcatga ttataagcgc tcaacatttg gcgaaataga
     6961 tgcattaact ttctttgaaa aagaaatggc gaacaaaaaa ctaatagtgt caccatgttg
     7021 ctatgttttt aaaagggagt tattaaatgg caatgcattc attccgcata tatatcatga
     7081 ggataatcat tttatagcaa ccctgatttt tgacaattat tcaactatat gcctctcgtt
     7141 ggatgaggag ttatttctta ggagggtaag aaatgattcc gtaatgacgt ctaaaaaatc
     7201 aatgaggcac atcattggat attcaacatg catcgatgag cttacgaagt ataaaaaaat
     7261 aaaaggcata caaaacaaga gctttgatag atttgtgaat ggaatggttt tgtcgttatt
     7321 ctctatatcg gaaacaataa aaacaaagga agcaatgaac tatgtggcga gatataaaaa
     7381 acaccccggc ctatcattta aagatagcgt atcaattaat tacccattaa ttaaaaagac
     7441 ttatttaaaa ctaaaaaacg ggaaggtgtt taattaccta aggaatggca ttattaataa
     7501 aaaaagcaac ccttaataaa agcaaagcga taattaataa aggtttttct ttgttgaata
     7561 aataagattt cggataggta tccccgtgac gggttgggtt ttcaggcgat acgcacgctt
     7621 tccggggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcggggtcag
     7681 tttggagatc ggaaattttt gtacgttaag cgcattattt aaccaatctt ggttcgtaag
     7741 gggttgattt tgcgtcagtg gctatcgctt ttaagttgtc gctcttgcag tatcgcattt
     7801 aggttatccg attaaggtca aacctctgaa aatgtcgtat agcgcgggag tacaccctat
     7861 tgctataggt aagtctgttc aaaaaacagg cttaccgtac aataattctc tatatccaaa
     7921 ctgaccccag atcacgcatc ttcccgacaa cgcagaccgg gtaatgactc caacttactg
     7981 atagtgtttt atgttcagat aatgcccgat gaccttgtca tgcagctcca ccgattttga
     8041 gaacgacagt gacttccgtc ccagccttgc cagatgttgt ctcagattca gattcagatt
     8101 atgtcgctca atgcgctgag tgtaacgctt gctgat
//
