LOCUS       CP057374                6669 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB28-C15 plasmid pRHB28-C15_4, complete
            sequence.
ACCESSION   CP057374
VERSION     CP057374.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148271
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T2-C15
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 165.998511090652x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:18:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,909
            CDSs (total)                      :: 4,793
            Genes (coding)                    :: 4,638
            CDSs (with protein)               :: 4,638
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 59 of 155
            Pseudo Genes (incomplete)         :: 84 of 155
            Pseudo Genes (internal stop)      :: 29 of 155
            Pseudo Genes (multiple problems)  :: 15 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB28-C15"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB28-C15_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(5866..6669,1..765))
                     /gene="cea"
                     /locus_tag="HVY54_24435"
     CDS             complement(join(5866..6669,1..765))
                     /gene="cea"
                     /locus_tag="HVY54_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="QMG65704.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            1025..1462
                     /locus_tag="HVY54_24440"
     CDS             1025..1462
                     /locus_tag="HVY54_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMG65705.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            1543..1962
                     /gene="exc2"
                     /locus_tag="HVY54_24445"
     CDS             1543..1962
                     /gene="exc2"
                     /locus_tag="HVY54_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMG65706.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(2280..2534)
                     /gene="mbeD"
                     /locus_tag="HVY54_24450"
     CDS             complement(2280..2534)
                     /gene="mbeD"
                     /locus_tag="HVY54_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMG65707.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(2541..3059)
                     /locus_tag="HVY54_24455"
     CDS             complement(2541..3059)
                     /locus_tag="HVY54_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="QMG65708.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mobilization protein MbeB"
     gene            complement(3037..3795)
                     /locus_tag="HVY54_24460"
                     /pseudo=""
     CDS             complement(3037..3795)
                     /locus_tag="HVY54_24460"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3737..4060)
                     /locus_tag="HVY54_24465"
     CDS             complement(3737..4060)
                     /locus_tag="HVY54_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMG65712.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4125..4316
                     /locus_tag="HVY54_24470"
     CDS             4125..4316
                     /locus_tag="HVY54_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMG65709.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5343..5480)
                     /locus_tag="HVY54_24475"
     CDS             complement(5343..5480)
                     /locus_tag="HVY54_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMG65710.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            5528..5869
                     /locus_tag="HVY54_24480"
     CDS             5528..5869
                     /locus_tag="HVY54_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="QMG65711.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(4402..4489)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          4500..5058
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.365"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2177..3747)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttgaccagc tcatccagtt ctttatattt agcggatgcc tgagccagtt catttcgttt
       61 tccagcgagc gttttcattt ctgcatcacg ggcatggata ctggagctta aacgagaatt
      121 gagagtctta atctctccat ccattttcac cacttcagat tgtgcagcag aaagtttttt
      181 ttgggcgatc tcaacagctt tagcttcttc actcaatgca gccagtcgtt tctcttcagc
      241 ttcagccagt ttcaactggc gttctgtttc agccttctcc cgttcaatct ctttacgtcg
      301 ttgttctgct tcctgaaaag ccttttctgc tgcttccgct tctttacggg ctttttcttc
      361 tgctttcgca aggcgcaaac gctctgcttc cgcctgcata gctgcattat tagcatgagc
      421 aagctctgtt gctgaaggcg tacgtgaggc attgtgacga agagcctcat tcacgatatc
      481 cttcaggcgc tgagtcagcg catccctgtt tgcctttgct ttcgcctgtg cttccgctgc
      541 agcttttgcc cgggcagcct gctctgcctg tgttttcttt aattgagcag tagaccattt
      601 agcagttgca tgaatagctg cagaactttc acttttactg cctccttttc cacctccgcc
      661 gccagagcca ctcccgtcag gagtaccatt caaaagagta ataattacct gtcccttatc
      721 atcataagga acaccatctt tatagtacgc taccgcggtt tccattataa aatcctcttt
      781 gacttttaaa acaataagtt aaaaataaat actgtacata taaccactgg ttttatatac
      841 agcataaaag ctacgccgct gcattttccc tgtcaagact gtggacttcc atttttgtga
      901 aaacgatcaa aaaaacagtc tttcacacca cgcgctattc tcgcccgatg ccacaaaaac
      961 cagcacaaac attaccgttc tcagacctca ttatgtttta ctgaaactat gagatgagac
     1021 atctatggga cactgtcact ttatggcatg gcacacactc cgggacgcac taaaaatgac
     1081 aggcagatcg cgttcacagt tttaccgtga tatgcgcgca ggccttgtca gttaccgtac
     1141 cggcagggac ggacgacggg agtttgaaac cagtgaactg atccgggcat acggcgaatt
     1201 aaagcagaat gagacaccag aaaggcacag tgagggacat gcagaaaatc cacatgatca
     1261 gcagacagaa cgcattctcc gggagctgaa tgagctgaaa caatgcctga cgctgatgct
     1321 tgaggataaa caggcacagg atatggatcg cagacgccag gaagcagaac gggaacagct
     1381 acaaaatgag atagcccagc tcaggcaggc actggaactg gaaaagaaac ggggattctg
     1441 gtccaggttg ttcggtcgct gaacgctgtc agagactgat gataaaatag tcttcggata
     1501 ataactcacc gagaataaat actttaaggt agggagacac tcatgagacg taccggaaac
     1561 aaactttgtc ttatcgccat gataacagca acagtagctc tcacagcctg taccccaaag
     1621 ggcagcgtgg aacaacatac ccggcattac gtatatgctt ctgatgacgg ttttgatccc
     1681 aacttttcca cccaaaaagc cgacacaaca cgaatgatgg tgcctttttt tcggcagttc
     1741 tgggatatgg gagctaaaga taaagcgaca ggaaaatcac ggagtgatgt gcaacaacgc
     1801 attcagcagt ttcacagcca ggaattttta aactcactcc ggggcacaac tcaatttgcg
     1861 ggtactgatt accgcagcaa agaccttacc ccgaaaaaat ccaggctgct ggctgacacg
     1921 atttctgcgg tttatctcga tggctacgag ggcagacagt aagtggattt accataatcc
     1981 cttaattgta cgcaccgcta aaacgcgttc agcgcgatca cggcagcaga caggtaaaaa
     2041 tggcaacaaa ccacccgaaa aactgccgcg atcgcgcctg ataaatttta accgtatgaa
     2101 tacctatgca accagagggt acaggccaca ttacccccac ttaatccact gaagctgcca
     2161 tttttcatgg tttcaccatc ccagcgaagg gccatccagc gtgcgttcct gtatttccgg
     2221 ctgacgctcc cgttccaggg ataacacatg ttcgcgctcc tgtatcagcc gttcctctct
     2281 tatctccagt tctcgctgta taactggctc aagcgttctg tctgctcgct caagcgttct
     2341 gtctgctcgc tcaagtgctg cacctgctga ctcaactgca tgacccgctc gttcagcatc
     2401 gcgttgtccc gttgcgtaag cgaaaacatc ttctgcaatt ccacgaaggc gctctcccat
     2461 tcgctcagcc gctgcatata gtcctgttgc agctgctcta aggcgttcag caaatgtgtt
     2521 tccagctctg tcactctgtg tcactccttc agatgtaccc actctttccc ctgaaaggga
     2581 atcacctccg ctgatttccc gtacggaagg acaaggaatt tcctgttccc gtcctgcaca
     2641 aactccacgc cccatgtctt cgcgttcagt ttctgcaatg tctcttcctg cttcctgatt
     2701 tcttccaggt tcgcctgtat cctccctcca agataccaga gcgtcccgcc actcgcggta
     2761 aacaggagaa agactatccc cagtaacatc atgcccgtat tccctgccag ctttaacacg
     2821 tccctcctgt gctgcatcat cgcctctttc accccttccc ggtgtttttc cagcgattcc
     2881 tctgtcgagg ctgtgaacag ggctatagcg tctctgattt tcgtctcgtt tgatgtcaca
     2941 gcctcgctta cagattcgcc gagcctcctg aactcgttgt tcagcatttt ctctgtagat
     3001 tcggctctct ctttcagctt tttctcgaac tccgcgcccg tctgcaaaag attgctcata
     3061 aaatgctcct ttcagcctga tattcttccc gccgttcgga tctgcaatgc tgatactgct
     3121 tcgcgtcacc ctgaccactt ccagccccgc ctcagtgagc gcctgaatca catcctgacg
     3181 gccttttatc tctccggcat ggtaaagtgc atctatacct cgcgtgacgc cctcagcaag
     3241 cgcctgtttc gtttcaggca ggttatcagg gagtgtcagc gtcctgcggt tctccggggc
     3301 gttcgggtca tgcagcccgt aatggtgatt caccagcgtc tgccaggcat caattctagg
     3361 cctgtctgcg cggtcgtagt acggctggag gcgttttccg gtctgtagct ccatgttcgg
     3421 aatgacaaaa ttcagctcaa gccgtccctt gtcctggtgc tccacccaca ggatgctgta
     3481 ctgatttttt tcgagaccgg gcatcagtac acgctcaaag ctcgccatca ctttttcacg
     3541 tcctcccggc ggcagctcct tctccgcgaa cgacagaaca ccggacgtgt atttcttcgc
     3601 aaatggcgtg gcatcgatga gttcccggac ttcttccgga ttaccctgaa gcaccgttgc
     3661 gccttcgcgg ttacgctccc tccccagcag gtaatcaacc ggaccactgc caccaccttt
     3721 tcccctggca tgaaatttaa ctatcatccc gcgccccctg ttccctgacg gccagacgca
     3781 gccggcgcag ctcatccccg atggccatca gtgcggccac cacctgaacc cggtcaccgg
     3841 aagaccactg cccgctgttc accttcctcg ctgtctgatt caggttattc ccgatggcgg
     3901 ccagctgacg cagtaacggc ggtgccagtg tcggcagctt cccggaacgg gcaaccggct
     3961 cacccaggca gacccgccgc atccatactg ccagttgttt accctcacag cgttcaagta
     4021 acctggcatg ttcatcatca gtaacccgta tcgtgagcat cctctcgcgt ttcatcggta
     4081 tcattacccc atgaacagaa ataccccctt acacggaggc atcagtgact aaacaggaaa
     4141 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctg ctggagaagc
     4201 tcaacgaact ggacgcagat gaacaggcag acatctgtga atcgcttcac gaccacgccg
     4261 atgagcttta ccgcagctgc ctcgcacgtt tcggggatga cggtgaaaac ctctgacaca
     4321 tgcagctccc ggagacggtc acagcttgtc tgtgagcgga tgccgggagc agacaagccc
     4381 gtcagggcgc gtcagcaggt tttagcgggt gtcggggcgc agccctgacc cagtcacgta
     4441 gcgatagcgg agtgtatact ggcttaacca tgcggcatca gtgcggattg tatgaaaagt
     4501 acgccatgcc gggtgtgaaa tgccgcacag atgcgtaagg agaaaatgca cgtccaggcg
     4561 cttttccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcggt
     4621 actgactcac acaaaaacgg taacacagtt atccacagaa tcaggggata aggccggaaa
     4681 gaacatgtga gcaaaagacc aagaacagga agaaggccac gtagcaggcg tttttccata
     4741 ggctccgccc ccctgacgag catcacaaaa atagacgctc aagtcagagg tggcgaaacc
     4801 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4861 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4921 tttctcatag ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4981 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5041 ttgagtccaa cccggtaagg cacgccttaa cgccactggc agcagccact ggtaaccgga
     5101 ttagcagagc gatgatggca caaacggtgc tacagagttc ttgaagtagt ggcccgacta
     5161 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5221 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gttggtagcg gtggtttttt
     5281 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5341 ttctactgaa ccgcgatccc cgtcagttta gaagaggagg atggtgcgat ggtccctccc
     5401 tgaacatcac gtatatagtt agcctgacat ccaacaagga ggtttatcgc gaatattccc
     5461 acaaaaaatc ttttcctcat aactcgatcc ttataaaatg aaaagaatat atggcgaggt
     5521 ttaatttatg agcttaagat actacataaa aaatatttta tttggcctgt actgcacact
     5581 tatatatata taccttataa caaaaaacag cgaagggtat tatttccttg tgtcagataa
     5641 gatgctatat gcaatagtga taagcactat tctatgtcca tattcaaaat atgctattga
     5701 atacatagct tttaacttca taaagaaaga ttttttcgaa agaagaaaaa acctaaataa
     5761 cgcccccgta gcaaaattaa acctatttat gctatataat ctactttgtt tggtcctagc
     5821 aatcccattt ggattgctag gactttttat atcaataaag aataattaaa tccctaacac
     5881 ctcatttata gtattaagtt tattcttatc aatataggag catagaattc ctgtaacaat
     5941 agcaataccc caaataccta atgtagttcc agcaagcaag ctaaaaagta aagcaacaac
     6001 ataactcacc cctgcatctg cagctttctt ctctaatgta agaaatagtg gcttccagtc
     6061 acctgtatcc ttaattttta ggatatcaga taccacatca tatccaaaag aaacatgccc
     6121 cgtaatcttc aagtacttgg caaactgatc taaatgttta gcccagtcat catacttcac
     6181 cgatgccaac gcattaaaaa tagcatcacg atcggctttg ctgaatttct tatttaaaac
     6241 atccttgtat ttttcaaaag cagcgagagc ttcattcaca ttgccgattt tcttaccttt
     6301 agacttatca gcaagttcct gtgccatttt cgaatatttt tcaccatatt tttcagtcag
     6361 cgtttgataa aagctaactg ttgcatcaac agcatcctta atctgtgaat taaggagatt
     6421 attctgtgct tttttcaaat tttcttcagc ttcatgaaca cgagcgatac cggcattacg
     6481 attattactg acctgagaaa tagccttctg gatctgagtt atatcagcat ttatccggtt
     6541 aatacgtgtt tctgatgctg ttacctgttt ttgtttttct tctctaatct taccggcccc
     6601 aacccgtcgt ctggttgctt caaaaaaagg acggttctga agcggatcat tggctcttgg
     6661 tgatagttt
//
