LOCUS       CP057366                8401 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB28-C20 plasmid pRHB28-C20_3, complete
            sequence.
ACCESSION   CP057366
VERSION     CP057366.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148273
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T2-C20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 169.962581629708x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:37:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,374
            Genes (coding)                    :: 4,177
            CDSs (with protein)               :: 4,177
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 82 of 197
            Pseudo Genes (incomplete)         :: 107 of 197
            Pseudo Genes (internal stop)      :: 28 of 197
            Pseudo Genes (multiple problems)  :: 19 of 197
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB28-C20"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB28-C20_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            442..591
                     /locus_tag="HVY56_22395"
     CDS             442..591
                     /locus_tag="HVY56_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG56608.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(1358..1798)
                     /locus_tag="HVY56_22400"
     CDS             complement(1358..1798)
                     /locus_tag="HVY56_22400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMG56609.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="entry exclusion protein 1"
     gene            2016..2405
                     /locus_tag="HVY56_22405"
     CDS             2016..2405
                     /locus_tag="HVY56_22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG56610.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            2449..2637
                     /locus_tag="HVY56_22410"
                     /pseudo=""
     CDS             2449..2637
                     /locus_tag="HVY56_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2604..3803)
                     /gene="tet(A)"
                     /locus_tag="HVY56_22415"
     ARG             complement(2608..3803)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3909..4559
                     /gene="tetR(A)"
                     /locus_tag="HVY56_22420"
     CDS             3909..4559
                     /gene="tetR(A)"
                     /locus_tag="HVY56_22420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="QMG56616.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(4591..4833)
                     /locus_tag="HVY56_22425"
     CDS             complement(4591..4833)
                     /locus_tag="HVY56_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="QMG56612.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(5139..5975)
                     /locus_tag="HVY56_22430"
     CDS             complement(5139..5975)
                     /locus_tag="HVY56_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="QMG56613.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(5975..6778)
                     /gene="aph(3'')-Ib"
                     /locus_tag="HVY56_22435"
     ARG             complement(5979..6778)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(6839..7654)
                     /gene="sul2"
                     /locus_tag="HVY56_22440"
     ARG             complement(6843..7654)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(5143..5975)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          234..407
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(3..267)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="99.0"
                     /evalue="3.95e-104"
     Typing          complement(7945..8400)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.368"
                     /coverage="99.0"
                     /evalue="1.08e-114"
ORIGIN
        1 accaggggga aacgcctggg atctttatag tcctgtcggg tttcgccacc actgatttga
       61 gcgtcagatt ttgcgatgct tgtcaggggg gcggagccta tggaaaaacg gcttcgccgt
      121 ggccttatct cttctttgct aagtgtcttc ttggtatctt ccaggaaatt tccgtccccg
      181 tccgtaagcc ggtaccgctc gccgcagccg aacgaccgag cgtagcgagt cagtgagcga
      241 ggaagcggaa tatctcctgt atcgcatatt ctgctgacgc gccggtgctg ccttttttct
      301 cctgccacat gaagcacttc actgattttc gcaatcgtgg caacatagta agccagtata
      361 cactccgcta gcgctacatg actggttcag ggctgcgccc cgaaacccgc taacaacctc
      421 tcagctactg gatatcaccc catgccatct gaatatgatg aacaactgga ttcgactgat
      481 acgcagcttc ccggcgaata tgaccttttt gatttaatgg cgcaggttac tgaagagaac
      541 ctgcatgccc ctgtggattt tgggtcgcct gtcggccggg aagcgttata gctttttcgc
      601 ttgcccgcgt acgtggtaat actccggtat taccttttat ttctttattt tcatgtaatt
      661 acagtggatt tttaataccc gggtattact cgtgaaaacg aaatattcct tttttttctt
      721 ttaattcatg cagttgcgat gaaaatcgcg gctgcgttag gtgtatgacc attttgaggt
      781 tgaaaaataa tcaaccatgg cgggtgattt acgcaggtta aggggagatc gtgacaaaaa
      841 aagcgttcct cactgtatcg ttcatgcctg catgaacggg ttattcttcc tgactacaga
      901 attacatcgc tccttcagtt catacatcac ttttacatca cttatacatc acttttacat
      961 cacttataca tcacttttac atcattcctt cagctcatac gtcactttca tcctctgtgc
     1021 catttgtaca tcaccccctt cctgaacgtg ctgacagcgg gttttccggc tgcgccggca
     1081 acgcttcacg gccacgcaat aaattgcgtc cccgttcacc gttttcggat gggaagaacg
     1141 ccggaaacag gacttacccc ggctgaatgg tgtgaatttc cgcgctatgc acttgtgcgc
     1201 atactcatgc atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt
     1261 gttgcctgtt ttaccggttt cccgtcagaa acgccctgag ggcctctcag gcggtgctga
     1321 taacagatgt tatggtaaat cagagtcacc tgcactgtta ctctgaaccg gagcgaaaca
     1381 gccgcgacca gaatccccgc tttttttcct gtgccagcgc gtcagccaga cggctaattt
     1441 cggcctgtag ttgttccctc tcctcatttc gcaggcggtt ttctgcttcc cgcgccatct
     1501 tgtcttcaag cattagggta acagtctgct gcagagcgtc cagtttctgc tgaatgacgt
     1561 ctagctgagc attttccgcc tggctaacga cagatttgga gtgtccctcc ttgtgccttt
     1621 ttggtgtctc agaagtgcgc agctcgccat aggcccgaat cagttcactg gtctcgaact
     1681 cccggcgatc attttttcct gtgcgatagc tgacaaggcc tttagccatg tcacgatata
     1741 attgcgagcg cgactttcct gttaatttca gtgtctcttg aactgtgtgc catgccataa
     1801 aagtgacagt gtctcctgtg tgtaccgtat tgtaacagca gaacatatta ctgttttaac
     1861 acaaaaggtc tgaggctgcc cgtgaggcgt gaaaaataca caaaaaacct gttgacggtg
     1921 tggttttgac gggcgtagta tttcgaatac tgtatataaa cacattgtta taaatacagt
     1981 ttttgctgtt tttaaaagtc aaagaggata attttatgag tacagagtta aatagcggta
     2041 ctgtcggtgg ttttggtcac atgggaccaa atgaccccta caatgatggt catagcggct
     2101 ctggaagttc gggaggtagt tttaacggtg gtggtaatgg tggccatgat aatagtagtg
     2161 gttcatctta tactgcatcc tttggtctca tcactggccg acacccgata actgtcccag
     2221 gtacgaagaa tattaattct gtcgatcttt ctgctgtcat taataacatt atacatcatg
     2281 aaggtgcacg caataacatg tacctagata ctgcgaacaa tgtaagagtg ggagtgacgg
     2341 gcactggctg gcaatgtcta gcaacggcag gcatttcggc tgagggtaaa agaactttcc
     2401 gctaagcgat agactgtatg taaacacagt attgcaagga cgcggaacat gcctcatgtg
     2461 gcggccagga cggccagccg ggatcgggat actggtcgtt accagagcca ccgacccgag
     2521 caaacccttc tctatcagat cgttgacgag tattacccgg cattcgctgc gcttatggca
     2581 gagcagggaa aggaattgcc gggctatgtg caacgggaat ttgaagaatt tctccaaagc
     2641 ccgcgacgca gcgccggcag gcagagcaag tagagggcag cgcctgcaat ccatgcccac
     2701 ccgttccacg ttgttataga agccgcatag atcgccgtga agaggagggg tccgacgatc
     2761 gaggtcaggc tggtgagcgc cgccagtgag ccttgcagct gcccctgacg ttcctcatcc
     2821 acctgcctgg acaacattgc ttgcagcgcc ggcattccga tgccacccga agcaagcagg
     2881 accatgatcg ggaacgccat ccatccccgt gtcgcgaagg caagcaggat gtagcctgtg
     2941 ccgtcggcaa tcattccgag catgagtgcc cgcctttcgc cgagccgggc ggctacaggg
     3001 ccggtgatca ttgcctgggc gagtgaatgc agaatgccaa atgcggcaag cgaaatgccg
     3061 atcgtggtcg cgtcccagtg aaagcgatcc tcgccgaaaa tgacccaaag cgcggccggc
     3121 acctgtccga caagttgcat gatgaagaag accgccatca gggcggcgac gacggtcatg
     3181 ccccgggccc accggaacga agcgagcggg ttgagagcct cccggcgtaa cggccggcgt
     3241 tcgcctttgt gcgactccgg caaaaggaaa cagcccgtca ggaaattgag gccgttcaag
     3301 gctgccgcgg cgaagaacgg agcgtggggg gagaaaccgc ccatcagccc accgagcaca
     3361 ggtcccgcga ccatcccgaa cccgaaacag gcgctcatga agccgaagtg ccgcgcgcgc
     3421 tcatcgccat cagtgatatc ggcaatataa gcgccggcta ccgccccagt cgccccggtg
     3481 atgccggcca cgatccgccc gatatagaga acccaaagga aaggcgccgt cgccatgatg
     3541 gcgtagtcga cagcagcgcc ggccagcgag acgagcaaga ccggccgccg cccgaaacga
     3601 tccgacagcg cgcccagcac aggtgcgcag gcaaattgca tcaacgcata cagcgccagc
     3661 agaatgccat agtgggcggt gacgtcgttc gagtgaacca gatcgcgcag gaggcccggc
     3721 agcaccggca taatcaggcc gatgccgaca gcgtcgagcg cgacagtgct cagaattacg
     3781 atcaggggtc tgttgggttt cacgtctggc ctccggacca gcctccgctg gtccgattga
     3841 acgcgcggat tctttatcac tgataagttg gtggacatat tatgtttatc agtgataaag
     3901 tgtcaagcat gacaaagttg cagccgaata cagtgatccg tgccgccctg gacctgttga
     3961 acgaggtcgg cgtagacggt ctgacgacac gcaaactggc ggaacggttg ggggttcagc
     4021 agccggcgct ttactggcac ttcaggaaca agcgggcgct gctcgacgca ctggccgaag
     4081 ccatgctggc ggagaatcat acgcattcgg tgccgagagc cgacgacgac tggcgctcat
     4141 ttctgatcgg gaatgcccgc agcttcaggc aggcgctgct cgcctaccgc gatggcgcgc
     4201 gcatccatgc cggcacgcga ccgggcgcac cgcagatgga aacggccgac gcgcagcttc
     4261 gcttcctctg cgaggcgggt ttttcggccg gggacgccgt caatgcgctg atgacaatca
     4321 gctacttcac tgttggggcc gtgcttgagg agcaggccgg cgacagcgat gccggcgagc
     4381 gcggcggcac cgttgaacag gctccgctct cgccgctgtt gcgggccgcg atagacgcct
     4441 tcgacgaagc cggtccggac gcagcgttcg agcagggact cgcggtgatt gtcgatggat
     4501 tggcgaaaag gaggctcgtt gtcaggaacg ttgaaggacc gagaaagggt gacgattgat
     4561 caggaccgct gccggagcgc aacccactca ctacagcaga gccatgtaga caacatcccc
     4621 tccccctttc caccgcgtca gacgcccgta gcagcccgct acgggctttt tcatgccctg
     4681 ccctagcgtc caagcctcac ggccgcgctc ggcctctctg gcggccttct ggcgctcctg
     4741 ctgcggcgtc cgctcgtggg ccgtggcgcg ggtccgcgcg ccggcctcgt gcgcctggcg
     4801 ctcgcgggcg aggtccaggg cggccgtctt cacgttctgc cttgcgcaga tgagatagat
     4861 ctagcgtgga ctcaaggctc tcgcgaatgg ctcgcgttgg aaactttcat tgacacttga
     4921 ggggcaccgc agggaaattc tcgtccttgc gagaaccggc tatgtcgtgc tgcgcatcga
     4981 gcctgcgccc ttggcttgtc tcgcccctct ccgcgtcgct acggggcttc caggggtcgt
     5041 ttgcgggaag gggcggaatc ctacgctaag gctttggcca gcgatattct ccggtgagat
     5101 tgatgtgttc ccaggggata ggagaagtcg cttgatatct agtatgacgt ctgtcgcacc
     5161 tgcttgatcg cggccgcgat agctagatcg cgttgctcct cttctccatc cgcgttccaa
     5221 gctgcggaaa ggcacccata agcgtacgcc tggtcgagca ggcgacgcgg atcgacgtcc
     5281 agcgcacgag agaatgcgtc cgccatctgt gcaatgcgtc taggatcgag acaaaggtcg
     5341 tctctgtcag ccggatcgta gaacatattg gcggcgccaa agcccacttc accgaccaga
     5401 ccgacgggat ctatcaccag ccagccgcga ctggagaaca tgatgttttc atgatgcaga
     5461 tcgccatgta gcccacgcag ttccgaggca ttgctcatca tttgatcggc tataatcgcc
     5521 gcgtggacgt agtcagtttg acaacctgcg ttttgatcat cgcgcgcccg ctgaaacaaa
     5581 gctgcaaagc gatcccggat cgggagaagg gcagaaggca ggggttcctc agatgcggca
     5641 tacagcttcg ccattagttc cgctgcaatt tcggtcgcct ggtagtcgcc gtgctcggca
     5701 acgatgtgag agagcattcg ctccccggca tattcgagca acatcagatt gttctcacga
     5761 ccgagcaacc ggactgctcc cctcccattg cgccatacca gatagtcggc cccgcgcagt
     5821 tcatcagcaa tgtcttctat aggtttcaat cccttgacga ttgcaggagt cccgtctggc
     5881 aatgaaactt tccaaacgag gctggaaaag gtgtccgcaa tgagaacagg ttgcgaaacg
     5941 tgccaatgag caggaaaaac aggcggcatg aacatcaacc ccaagtcaga gggtccaatc
     6001 gcagatagaa ggcaaggcgt tcgcggtcgg gggcttcgat ccccaataca ttgaatagga
     6061 cagcgaaggc gcgctctgct tcatctggcg ctgcccagtt ctcttcggcg ttagcaatca
     6121 tgagtgccaa atcggcatag cgatctgctg ttccgagccg cccaaggtcg atcagacccg
     6181 tgcattgaag agttttaggg tccaccatga agttcggcat gcagggatca ccatggcaaa
     6241 caaccatatc ggtgcgctct tggtcgagcc gcaccggtag ctctcgttcg acacgagcca
     6301 aaagatcgag ctgcggcgta ctcttgtcct cgtccggtaa gaagtcggga ttgacggcat
     6361 tgcgggacac cacatcaacg gcgcgtccga acattcgcga cagcctgcgc tcaaacggac
     6421 attgatcaac cgataggctg tgaacagcgc caagttgctg ccccattgac ggccacgctt
     6481 tgagcaaatc cgctccagac agatcagccg ccggtactcc cggaattgcc gttatcacca
     6541 agcatgcacc ctcctgttcc tcctgccagt tgatgacctc ggggcaagcc acacctcgac
     6601 ctttgagcca aatgaggcgg tcacgctctc cagcgagctc accgcggcgg gaagcaggtg
     6661 cgattttcgc gaaggcatgc ccgtcaccac gtcgaaaaac aaaatcacca gattctccgc
     6721 ctctgacagg caaccagtca gaatgcgatt caccaaaaaa aatattagtt cgattcaatg
     6781 gaggttcctt cagttttctg atgaagcgcg aatatagaga aatatcccga atgtgcagtt
     6841 aacgaattct tgcggtttct ttcagcgccg ccaataccgc cagcccgtcg cgcaaggggc
     6901 gcggctcgtg tgtgcggatg aagtcagctc cacctgcggc ggcggcaagc tctgcagcga
     6961 gtgtcgcggc cccgacatcc cccggaccac ggcctgtgag cgcgcgcaga aaggatttgc
     7021 gcgaaacaga cagaagcacc ggcaaatcga agcgcagccg caattcatcg aaccgcgcca
     7081 gcaccgagag cgaggtttcg ggagcagccc ccagaaaaaa ccccatgccg ggatcaagga
     7141 caaggcggtt gcgtttgata ccggcacccg tcagcgccgc gatgcgcgcg tcaaagaacg
     7201 ccgcaatgtg atccatgatg tcgccagcgg gtgcctcgcg ccgatctgcc tgcccgtctt
     7261 gcaccgaatg cataacgacg agtttggcag atgatttcgc caattgcgga tagaacgcag
     7321 cgtctggaaa accgcgaata tcattgagat aggccacacc acgcgacaag gcataggctt
     7381 gcgtcgcggg ttgataactg tcgagcgaga cgggaatgcc atctgccttg agcgcgtcca
     7441 gcaccggcgc gatacgcgcg atttctgtgt cggacgaaac aggcgcggcg tcgggattgc
     7501 tggatgccgg accgaggtcg atcacatctg ccccctcggc catcagctta cgcgcctgcg
     7561 caatggctgc gtctggcgcc agataccggc ctccatcgga gaaactgtcc gaggttatgt
     7621 tgacgatgcc gaaaatgatg agcgatttat tcatgggggc ttctataata ataataatcg
     7681 agcatgagtc tcatacggat gctcgggtcg aaagggaatc cccaggcgag taacctgttt
     7741 gcggtgatcc gtttcatgat gatgtatctg tagcattttg gaaataaata tatcatctct
     7801 caataagtag atatatttat cttttttgct tctttggtgc tgcaagtcac caatacgagg
     7861 gaaacctgtt acggagcggt ttaaccgtaa tcaacagtga ctggtgtcat gttcggaaga
     7921 ttaattgata gtaatctaca aaggatcttc ttgagatcct tttcaatgaa tcgtaatctc
     7981 ttgctctgta aacgaaaaaa ccgcctgggg aggcggtttg ttcgaaggtt aagtcagttg
     8041 gggaactgct taacccggta acagggttca cgagaccgcc attaccaaaa ctgttctttc
     8101 agtgtagccg cagtcaggcc tctacttcaa gacctcttaa tctatcttct cgtgcactcc
     8161 tgttactaat ggctgctgcc agtggcgttt tgtcgtgtct ttccgggttg gactcaagtt
     8221 gatagttacc ggacaaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca
     8281 gcttggagcg aactgccttc acggaactga gtgtcaggcg tggaatgaga aaccgcggcc
     8341 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc
     8401 t
//
