LOCUS       CP057365                9361 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB28-C20 plasmid pRHB28-C20_2, complete
            sequence.
ACCESSION   CP057365
VERSION     CP057365.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148273
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T2-C20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 169.962581629708x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:37:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,493
            CDSs (total)                      :: 4,374
            Genes (coding)                    :: 4,177
            CDSs (with protein)               :: 4,177
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 197
            CDSs (without protein)            :: 197
            Pseudo Genes (ambiguous residues) :: 0 of 197
            Pseudo Genes (frameshifted)       :: 82 of 197
            Pseudo Genes (incomplete)         :: 107 of 197
            Pseudo Genes (internal stop)      :: 28 of 197
            Pseudo Genes (multiple problems)  :: 19 of 197
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB28-C20"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB28-C20_2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(9154..9361,1..1868))
                     /locus_tag="HVY56_22360"
     CDS             complement(join(9154..9361,1..1868))
                     /locus_tag="HVY56_22360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="QMG56607.1"
                     /translation="MQTELIKELNKVLKAFPQFWSGENLHRSMVSDAISQRQPDLIKAL
                     VANEKVSSVYGTDIDGILIFDFDKLCSLLRYKEYWKNSFTKYRNKIGLTSSGKYISDST
                     DIVLDFPFKDCVLEGGMTKEDQGKEEIYYNEIIARDEIDNLLSPKALVNVSRHTKNGEV
                     KNIRDFHESDNLIIKGNNLIALHSLLTKFTGKVKLIYIDPPYNTGSDSFKYNDNFNHSS
                     WLTFIKNRLEVAKELLSEDGVIFVQSDDNEQAYLKVLMDNIFDRENFINMISVNMKNVA
                     GASGGGEDKRLKKNCEFILVYSKNYAAFSAFKDVFEYTEIHEVVEQYKKEGKNWHYSSV
                     LVNAGEKIYVTSTTDGSGNEIKIYKHPNAVIKSVKQLMLQEKISEKDVFYNFGTKIFEA
                     KDAQSSIRTRVIKAKKEHHINDDILSIEYIPKSGRNKGQVYEQFYKGDKCRLFAWLRDI
                     TENIDGVLYKRDKQGTYWDFTSHMNNLMKEGEISFQNGKKPEALIQRIIDISTNKNDIV
                     LDFHLGSGTTAATAHKMERRYIGIEQMDYINDITVPRLKNVITAENNGISRDVNWQGGG
                     SFVYAELMELNACFVHEIQKAQSSEELEKLFAVMKTEAHLNYQVALENVLSAEYEMDGI
                     PRKVAFSELELHEQKQLLIEILDKNQLYVNASDMDDSDLNISESDKAFTRSFYGME"
                     /name="site-specific DNA-methyltransferase"
     gene            complement(2153..2575)
                     /locus_tag="HVY56_22365"
     CDS             complement(2153..2575)
                     /locus_tag="HVY56_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="QMG56601.1"
                     /translation="MALVSITDAARLTGKSRRTIQRHIATGKLSVSHSDATGKLIETSE
                     LIRCYGEIKQSNVTPEKDMSQMTMSHDVTLLIDKNESEIELLKQQVALLQQRLQDKDEH
                     ISSLKQAMLLIESKLPATPDPARQPAVKKSWQFWKK"
                     /name="DNA-binding protein"
     gene            complement(2583..3542)
                     /locus_tag="HVY56_22370"
     CDS             complement(2583..3542)
                     /locus_tag="HVY56_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMG56602.1"
                     /translation="MSELVVFKANELVISRYDLTEHETKLILFSVAKLNPTIETPDEAQ
                     RTVIFSCSEYAQVMNLSYENAWGRLNSSTSDLFKRSVELIYPSGDVAKRVFNWAEYAEF
                     NRKNQTVTLIFSKYIVPFLFHLKKFIKYNLEHVKSFENKYSMRIYEWLLKEMSQRKTNN
                     ANIEISLKEFKFMLMLENNYPEFKFLNTRVLKPIIKDLNTYSNMQLEIEKRGRPVDTLI
                     FQVKLDKQIDLVTELAKNSPSKTSDQIPLSITPEAYLHKGLRKTLHDALTAKIQLTDFE
                     SKFLHDMQSKHDLNGSFSWLTSKQRNTLEKILAKYGQI"
                     /name="replication initiation protein"
     gene            complement(4147..4614)
                     /gene="traD"
                     /locus_tag="HVY56_22375"
     CDS             complement(4147..4614)
                     /gene="traD"
                     /locus_tag="HVY56_22375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="QMG56603.1"
                     /translation="MTQSEKQQKWITDRVQYIRGLKSPNEQQRLLIFLTDKTDRTAQEN
                     KTLSLLIQAEKAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLIMAGLV
                     DSKTGKPVDDAAALLGALAGLNDLSRDNPKWSDWKIRGQVLLKRKKADAQA"
                     /name="traD"
     gene            4782..5873
                     /gene="mobA"
                     /locus_tag="HVY56_22380"
     CDS             4782..5873
                     /gene="mobA"
                     /locus_tag="HVY56_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="QMG56604.1"
                     /translation="MASYHLSVKTGGKGRASPHANYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTLERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERLNDGFDRDPQQYFKRANSKAPERGGAKKERFG
                     ETPTERKVHLVALRERWADLQNQYLERYQYADRVDARSLKAQGIEREPERHLGAGQVQR
                     FDIEQLHAILERRDAERQAQQSFYERDSVIDVTTLLREAISERDNLAPKQDQTPEQEAH
                     SVRTFDFENASEKLNNLVSDALKDVQDEIDLQSLVNDAMAEFQGIHQEMERQKERERQQ
                     EKERQRLAEHTQQKPDKGWSFSR"
                     /name="mobA"
     gene            5886..6470
                     /locus_tag="HVY56_22385"
     CDS             5886..6470
                     /locus_tag="HVY56_22385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="QMG56605.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVISLTEQFQDASAQLQQQSETLSR
                     LIRELDSASGNMAETVRKSVNSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(6513..9149)
                     /locus_tag="HVY56_22390"
     CDS             complement(6513..9149)
                     /locus_tag="HVY56_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="QMG56606.1"
                     /translation="MAKKPKSAGSDKLLFNKLRQLDEPGLFHDNYQTPDYIQANLRDEL
                     RPYQHGALRYLHYTQRRQEETLLHYRHLLFHMATGAGKTMVMAGTILYLFKEYGYQNFI
                     FFVHTDAIIQKTRENLLNPQSTKYLFSQELEIDGEKITIAPVETFPSAPERNTIYLKLS
                     TIHKMHDELNSYRENSITYEDLKEIPLVLLGDEAHHFNAGTKAKGKSKTSPENEEQTWE
                     RTIENILALRPDNRLFEFTATIDLANKDIGQKYRDKVVYQYDLKQFMSDGYSKKVMLLE
                     ANQNDGDKMLDAVLLSQYRKLIATDNGITGFKPVILFKSNKIAISKAKQEEFSQLIAAM
                     TPESIRRHLANKKVQLSSDTSIWHKVIQRYSGSDLVTVIGQIQEDFNDFNLLNVNKSDL
                     LEENPVLLNTLEEVDNPVRAVFAVAKVNEGWDVLNLYDIVRISEQASSSKTGTDSEAQL
                     IGRGARYFPFVYDGKRSFTRRFDNSAKDLSVLEQLHYHTINEPAYIKTLHASLEQADID
                     VHQDGGGTIEHARLKEDFKKSAVYQMGRLYFNEVEEIESNSRNWETYSLETRFEIPYQT
                     AGEESLDNLTGTTAGITKPEPLVLDERFYRKAMQRISFYALDNLRRFFPKLTGIREFIH
                     SDAYLGKLKITVTVPQSLDFSTVPAKEKLHLLETVLLRISENVRRNDQKVKGTYRFISQ
                     PVKEVIKDYSLHIDPSVVINQKITAVPTIGKKWYVYDNAILNQLEHRLVKTLEAFIPKL
                     RARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTCHADNAYWQVFLEPKGDDRL
                     LDDAWKERMLETLNDRERIVIDENENVRLVGIKFFANSQMDAFVCDMQNRLNDGESLET
                     ASLSLPL"
                     /name="DEAD/DEAH box helicase family protein"
     Typing          4783..5870
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tccagtgcca cctgatagtt gagatgtgct tcggttttca tcaccgcaaa aagtttttcg
       61 agttcctcac tggactgtgc tttctggatt tcatgcacaa agcaggcatt cagctccatc
      121 agttcagcgt aaacaaagct gccgcccccc tgccagttta cgtctcttga aattccgttg
      181 ttttcagccg tgattacatt ttttagtcga ggaaccgtta tgtcattgat gtaatccatt
      241 tgttcaatgc caatgtaacg acgttccatt ttatgtgctg ttgcagcggt tgtcccgctt
      301 ccgagatgga aatctagtac aatatcgttt ttatttgttg aaatatctat aattctttgt
      361 attagtgcct ctggtttttt tccattttga aatgatattt caccttcctt cattaggtta
      421 ttcatgtgtg acgtaaaatc ccaatacgtt ccttgtttat ctcttttgta taaaacgcca
      481 tctatatttt cagttatatc tctaagccag gcaaacaatc tgcatttatc acctttgtag
      541 aattgttcat atacttggcc tttattcctt cctgattttg gaatatattc tattgataat
      601 atgtcatcat taatgtgatg ttcttttttt gcttttatca ctcgcgttct aatggatgat
      661 tgagcatcct ttgcttcaaa tatttttgtg ccaaagttat aaaagacatc cttctctgat
      721 attttttcct gtaacatcaa ttgtttaact gatttaatta ctgcgttagg atgtttgtat
      781 atttttattt cgtttccgct gccatcagta gtagatgtta cgtatatttt ttcccctgca
      841 tttaccagca cagaggagta gtgccagttt ttaccttctt tcttatattg ttcaactact
      901 tcatgaattt cggtgtattc aaaaacatct ttaaatgcag aaaaggcagc atagtttttt
      961 gagtaaacta aaataaactc acagtttttt ttaagtcttt tatcctcgcc gccaccagaa
     1021 gctccggcaa cattcttcat attcactgat atcatattga taaagttttc tctatcaaaa
     1081 atgttatcca ttaaaacttt taaatatgct tgttcattgt cgtctgattg tacaaatata
     1141 actccatctt cactaagaag ttcttttgct acttctagcc tattttttat aaatgttagc
     1201 catgatgaat gattgaaatt gtcattgtat ttaaaactgt cagatcctgt attgtatggc
     1261 gggtctatat atattaattt cacttttcca gtgaattttg tcaatagtga gtgaagtgca
     1321 attaagttat ttccctttat tattaagttg tcactttcat gaaaatcacg aatgttttta
     1381 acctctccat tctttgtatg gcgagataca ttaaccaatg cttttgggga tagtaaatta
     1441 tctatttcat cgcgagcaat tatctcgtta taataaatct cttctttccc ttgatcttct
     1501 tttgtcattc cgccttcaag aacacaatcc ttgaaaggaa aatcaagcac tatatcagtg
     1561 ctgtcgctaa tgtatttgcc tgaactggtt aatccaattt tattacggta tttagtaaaa
     1621 ctgtttttcc agtattcttt atatcttaac agactgcaaa gtttgtcaaa atcaaatatt
     1681 aaaatgccat caatatctgt accgtaaaca gaactgactt tttcattagc gactaatgct
     1741 ttgattaggt ctggctgcct ttgactgatg gcgtcactca ccattgaacg gtgtaaattt
     1801 tctccgctcc agaattgtgg gaacgctttt aaaaccttgt tcagctcttt gattaattcg
     1861 gtctgcatgt catggcctta tgactgttag agattgaaat gattgtaagg gatcgcgtcc
     1921 gatgctggaa cctgacagcg atgacacaga tacacttgcg gaaaaatgca ccaaagagaa
     1981 ggtgtttaac ataatggatc ttgcgcgcac cactgatcag gcactcatac ccagaacccg
     2041 gcaaaacgtc caaaagtgca ggttttgacg cccgaaaacg ggctgttttg gtgttgctcg
     2101 tttgttatca ggatcgaggt tccggtttca gccggtttgc cggctgaaac cgctatttct
     2161 tccagaactg ccatgatttt tttaccgcag gctgtctggc gggatcgggg gttgctggga
     2221 gttttgattc gataagcagc attgcctgtt ttaggctgct tatgtgttca tctttatctt
     2281 gaagccgctg ctgcaataag gcaacttgct gctttagtaa ctctatttct gattcatttt
     2341 tgtctatcag tagggtgaca tcgtgtgaca ttgtcatttg tgacatgtct ttttctggtg
     2401 tgacattact ttgttttatc tcaccataac accttattaa ctctgatgtt tctatcaatt
     2461 ttcctgtcgc gtcagaatgt gacactgaca gttttcctgt cgctatgtgt cgctgtatag
     2521 tcctgcgact ctttccagtt aatcttgctg cgtcagttat ggataccaga gccatgacta
     2581 cctcaaattt gtccgtattt cgctaatatc ttttccagag tgttgcgttg ctttgatgtt
     2641 agccaagaaa aagaaccatt aagatcatgt ttactttgca tatcgtgaag aaactttgac
     2701 tcaaagtcag tgagttgaat cttagcggtt agtgcatcgt gtagggtttt tcttagcccc
     2761 ttatgtagat aggcttctgg tgtgatgctc agcgggattt gatcactagt tttactaggt
     2821 gagtttttgg ctaactctgt tactaagtca atttgcttat ctaacttaac ctgaaatatc
     2881 aatgtatcta caggacgacc acgtttttca atttctaact gcatattgct ataagtgttc
     2941 aagtctttaa ttatcggttt taaaactctt gtatttaaaa acttaaattc agggtaatta
     3001 ttctcaagca ttagcatgaa tttaaattct tttaaactta tctcaatgtt ggcattgttt
     3061 gttttacgtt gactcatttc cttaagtagc cattcataaa tacgcatgga gtatttgttt
     3121 tcaaatgact ttacatgctc gagattgtat tttatgaact ttttcaaatg aaacaaaaaa
     3181 ggaactatat acttactaaa gatcaacgtt actgtctggt ttttacgatt gaactcggca
     3241 tactcagccc agttaaaaac tcgcttagct acatcaccac tagggtaaat cagctccacg
     3301 gatcgcttaa ataaatcact cgtagatgaa tttaatcgtc cccacgcatt ttcataacta
     3361 agattcatta cttgagcata ttcagaacat gaaaaaatga ctgttctttg cgcttcatca
     3421 ggagtctcta ttgttggatt cagctttgcc acactaaata aaatcagttt agtttcatgt
     3481 tccgtgagat cataacgact gataacaagc tcatttgctt taaatacaac tagttctgac
     3541 atgacagacc ctatctctac gcaaaggtat acagattaag atagagcaac cataggtaaa
     3601 ggtaaacgat ttttttatct gtatacctat cggcagctgg ctgtggatag cctaattttt
     3661 tgtataccct ttggctgaaa aacctaattt tttgtatacc cagcctaatt ttttgtatac
     3721 cttttgcatt tttttttctt ttgaaacaag agcttaaaac tgtaagaata aagaaagaat
     3781 agaaaaaaat aaaaagaata gatcccagcc ctgtggataa cagaacgact gagacagttg
     3841 tcctatatga caaaaggatg tcgcaaacgc tgtttgctcc ccttcagaac agacctttaa
     3901 acctttaaga ctgggtagca ccctcgcaga ctcgggcaaa tcgcggaaca ttccttttgt
     3961 ctccgaccat cagacacctg aaacgctgct cttttcgtgg gattcagttc gctccgctca
     4021 cggctctggc agggagtggg ggtaaatggc gctccagccg cttatcaggc attttagtgg
     4081 atgattccac tagtgaagat gaaaaagccc gtagcgggct tctcagagcg ttttatgacg
     4141 ggtctgttat gcctgagcgt ctgccttttt tcttttcagc agtacttgac ccctgatttt
     4201 ccagtctgac cacttcggat tatcccgtga caggtcattc aggccggcta atgctcccag
     4261 cagggcggca gcatcatcaa ccggtttccc cgtcttgctg tctaccagac ccgccatgat
     4321 aagcagtccg gctgactgat acagggcatg gtccctggct ttacgggcgg ctctggcttc
     4381 agcacgtttt tcggcctgta tcaggttcat cacttttgct ctggcttcct gagctttctc
     4441 tgctgccttt tctgcctgaa tcagcaggga gagagtttta ttttcctgtg ctgttctgtc
     4501 ggttttatcg gtcagaaaaa tgagaagtcg ctgttgttca ttgggcgact ttaaaccacg
     4561 gatatactgc actcggtctg ttatccattt ttgctgtttt tcgctttgag tcatttctgt
     4621 ttatcctctg aatattgacg gggaaattat ggtcaaaaat ggcggatgga taagatcaaa
     4681 tgtgggtaga atcactgata agctttttat tatcagtgcg cacttagacg tttcttcact
     4741 ttgttcagaa acggtgcgcg ccttcagcgg gataaaacac gatggcatcc tatcatctca
     4801 gcgtaaaaac cggcgggaaa ggccgtgcct ctccccatgc caattatatt gcgcgtgagg
     4861 gaaagtatgc ccgtgaaaaa gactccgatc ttgagcataa ggagtccggt aatatgcctg
     4921 cgtgggcagc gcataaaccg tccgagttct ggaaagccgc tgacacgtta gagcgtgcca
     4981 acggctgcac ttaccgcgaa atagaaattg ccctgccgcg tgagctgaaa cctgaacaac
     5041 gccttgagct ggtacgcgat ttcgttcagc aggaaatcgg cgaccgtcat gcgtaccagt
     5101 ttgctatcca taatccgaaa gcggcgatag ccggcggcga gcagccgcac gcgcatatca
     5161 tgttctctga gcgcctcaat gacgggtttg accgtgaccc gcagcagtat ttcaaacggg
     5221 ctaatagcaa agcccctgag cgcggtggtg cgaaaaaaga acgtttcggc gagacgccga
     5281 ctgaacgcaa ggtgcatctt gtcgcactgc gtgagcgctg ggctgaccta caaaatcagt
     5341 atcttgaacg gtatcagtat gctgaccgtg tcgatgctcg ctccctgaaa gctcaaggca
     5401 ttgagcgtga gccggagcgg cacttaggtg cgggacaggt tcagcggttc gatattgagc
     5461 agttgcacgc catccttgag cgcagggatg ctgaaagaca ggcgcagcag tctttttatg
     5521 agcgtgacag cgtcattgac gtcaccacct tgctacggga agccatcagt gaacgggata
     5581 atctggctcc caaacaggat cagacacctg agcaggaagc ccattccgtc agaacatttg
     5641 attttgaaaa cgcgtcggaa aagctgaaca acctggtcag tgatgcactg aaggacgtac
     5701 aggacgaaat cgaccttcag tccctggtta acgatgcaat ggcggagttt caggggatac
     5761 atcaggaaat ggaacgacag aaagaacgcg agcgtcagca ggagaaagaa cggcagagac
     5821 ttgccgaaca tacacaacag aagccggata aaggctggtc attttcacga tagacaggat
     5881 tttgcatgag tgacgaaatt aaacaaatcg ccgcccttat cgggcatcat caggcgctgg
     5941 aaaagcgtgt tatctctctg actgagcagt ttcaggacgc ctcagcacaa ttacagcagc
     6001 agagtgaaac gctgagcagg ctcattcgtg agctggacag cgcgtccggt aatatggctg
     6061 aaaccgtcag gaaatcggtt aattccgcgc tcactcaggt tgagaaagaa ctgaaacaag
     6121 ccggattagc acagcagaaa ccagcgacag aagcgcttaa tcaggctgcc gatacggcga
     6181 aggcaatgat ccatgaaatg cgccgcgaga tgtcgcgcta tacgtggaaa tccgcgattt
     6241 atcttgtgct gacgatattc ttcgttctgg ctgcctgcgt gacggcattc acgtggttta
     6301 tgaatgacgg gtacagccag attgctgaaa tgcagcggat ggaagccgtc tggcagaaga
     6361 aagccccgct agctgatata tcgcgttgtg acggaaagcc ctgcgttaag gtggacactc
     6421 gcaccacgta tggtgataaa gagaatacct ggatgattat caaaaaataa aataacggcg
     6481 cacagtgcgc cgttatttgt atttgtcact atttacagtg gcagggaaag cgaggcggtt
     6541 tcaagcgatt cgccgtcatt cagcctgttc tgcatgtcgc agacgaaggc atccatctga
     6601 ctgttggcaa agaatttaat tccgaccagc ctgacgtttt cattttcatc aatcacaatg
     6661 cgctccctgt cattcagggt ttcgagcatc cgctctttcc atgcgtcatc cagcagacgg
     6721 tcatccccct tcggctcaag gaagacctgc cagtaagcat tatccgcatg gcacgtcagt
     6781 atcatgatga agtccggcat aaagccacgt acgccgccaa attccgtgag cttaaagcgc
     6841 gttgactgct cgtcgttacg cagcacgtaa atatcgtcgt aacgtgctct gagcttcggt
     6901 ataaaggctt ccagagtttt caccagacgg tgctcaagct gattcagtat cgcgttatcg
     6961 tacacgtacc attttttacc aatggtgggt acggcggtga ttttctggtt aatgactacc
     7021 gacgggtcaa tgtgcaggct gtagtcttta atcacctctt tcaccggctg gctgataaag
     7081 cggtacgtgc ccttcacttt ctggtcattg cggcgcacgt tctctgagat acgtagcaga
     7141 actgtttcca gcaggtgcag tttctctttt gccggtacgg tggagaaatc cagtgactgc
     7201 ggcacggtca cagtgatttt cagtttgcca agataggcgt cactgtggat gaactcacgg
     7261 atgccggtca gtttcgggaa gaagcgtcgc aggttatcca gtgcgtagaa gctgatcctc
     7321 tgcatcgcct tacggtaaaa acgctcgtcc agtaccagtg gttccggttt tgttatgcca
     7381 gccgttgtgc ccgtcaggtt gtccagcgac tcctcaccgg cggtctgata cggtatctca
     7441 aagcgcgttt ccagtgagta ggtttcccag ttgcggctgt tgctctcaat ctcttccact
     7501 tcgttgaaat acagcctacc catctgatac acagcggatt ttttaaagtc ctctttcagt
     7561 ctggcatgtt caattgtgcc gccaccgtcc tgatgcacgt caatgtccgc ctgctccaga
     7621 gatgcgtgca gcgttttgat gtacgccggt tcattgatgg tgtggtagtg cagttgttcc
     7681 agcacactta aatctttcgc gctgttatca aagcggcggg tgaagctgcg tttgccgtca
     7741 tacacgaacg ggaagtaacg tgcaccgcga ccgataagct gtgcctcgct gtccgtgccg
     7801 gttttgctgc tgctcgcctg ttcgctgatg cggacaatgt cgtagaggtt gagtacatcc
     7861 cagccttcat tgaccttcgc cacggcaaag acggctctca ccggattatc gacttcttcc
     7921 agcgtattca gcaggacggg gttttcttcc agcaagtcgg atttattgac gttcagcagg
     7981 ttgaagtcgt taaaatcttc ctgaatctgc ccgataaccg ttaccaaatc gctgccagag
     8041 taacgttgaa tcactttgtg ccagatactg gtgtcagagc tgagctggac ttttttattt
     8101 gccagatgac gtctgatgct ctctggcgtc attgccgcaa tcagttgcga gaactcctcc
     8161 tgcttcgcct ttgatatcgc aatcttattc gacttgaaca gaatgaccgg tttgaagccg
     8221 gtgatgccgt tatctgtcgc tatcagttta cggtactggc ttagcagcac ggcatcgagc
     8281 atcttgtcgc cgtcgttctg attcgcttcc agcagcatga ccttcttcga gtaaccgtct
     8341 gacatgaact gcttcaggtc atactgatac accaccttgt cacggtattt ctgcccgata
     8401 tctttgttcg caaggtcaat ggtggcggtg aactcgaaca aacggttatc cggtcgcagg
     8461 gctaggatgt tctctatcgt acgctcccag gtctgctcct cattctccgg tgaagttttc
     8521 gacttgcctt tggctttggt gccggcgttg aagtgatgcg cctcgtcacc gagcagcacg
     8581 agcgggattt ccttcaaatc ctcgtaggtg atgctgttct cgcgatagct gttcagctcg
     8641 tcgtgcatct tatggatggt cgagagctta aggtaaatcg tgttgcgctc aggtgcagac
     8701 gggaacgtct ccaccggtgc gatggtgatt ttctccccgt cgatttccag ctcctgactg
     8761 aacaggtact tcgtcgattg cgggttgagc agattctcac gggttttctg aataatcgcg
     8821 tcggtatgca cgaagaagat gaaattctga tacccgtact ctttaaacag atagagtatc
     8881 gtccccgcca tcaccatcgt ttttcctgca ccggtagcca tgtgaaatag cagatgccgg
     8941 taatgcaata gcgtctcttc ctgcctgcgc tgcgtgtaat gcaggtagcg cagcgccccg
     9001 tgctggtacg ggcgcagttc atccctcagg ttcgcctgaa tgtagtccgg cgtctggtag
     9061 ttatcatgga acagccctgg ttcatcgagc tgtctgagct tgttaaacag cagcttgtcg
     9121 ctgcctgctg actttggttt cttcgccata ccgttactcc attccgtaga agctgcgggt
     9181 aaaggctttg tcgctctctg agatgttcag gtcgctgtca tccatatcgg aagcgttcac
     9241 atagagctgg tttttatcga ggatttcaat cagtagctgt ttctgctcat gcagttccag
     9301 ctcgctaaac gcgactttgc gcggaatgcc gtccatctcg tactcagcag acagcacgtt
     9361 t
//
