LOCUS       CP057351                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB29-C08 plasmid pRHB29-C08_3, complete
            sequence.
ACCESSION   CP057351
VERSION     CP057351.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148278
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH11 T3-C08
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 154.904905608821x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:04:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,807
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,597
            CDSs (with protein)               :: 4,597
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 37 of 96
            Pseudo Genes (incomplete)         :: 58 of 96
            Pseudo Genes (internal stop)      :: 15 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB29-C08"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB29-C08_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(179..370)
                     /locus_tag="HVY61_23975"
     CDS             complement(179..370)
                     /locus_tag="HVY61_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMG39025.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            435..758
                     /locus_tag="HVY61_23980"
     CDS             435..758
                     /locus_tag="HVY61_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMG39034.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            916..1458
                     /locus_tag="HVY61_23985"
                     /pseudo=""
     CDS             916..1458
                     /locus_tag="HVY61_23985"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1436..1954
                     /locus_tag="HVY61_23990"
     CDS             1436..1954
                     /locus_tag="HVY61_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMG39026.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            1961..2194
                     /gene="mbeD"
                     /locus_tag="HVY61_23995"
     CDS             1961..2194
                     /gene="mbeD"
                     /locus_tag="HVY61_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMG39027.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(2514..2936)
                     /gene="exc2"
                     /locus_tag="HVY61_24000"
     CDS             complement(2514..2936)
                     /gene="exc2"
                     /locus_tag="HVY61_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMG39028.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            3058..3336
                     /locus_tag="HVY61_24005"
     CDS             3058..3336
                     /locus_tag="HVY61_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39029.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(3328..3618)
                     /locus_tag="HVY61_24010"
     CDS             complement(3328..3618)
                     /locus_tag="HVY61_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39030.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            3859..5358
                     /gene="csa"
                     /locus_tag="HVY61_24015"
     CDS             3859..5358
                     /gene="csa"
                     /locus_tag="HVY61_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QMG39031.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            complement(5386..5925)
                     /gene="csi"
                     /locus_tag="HVY61_24020"
     CDS             complement(5386..5925)
                     /gene="csi"
                     /locus_tag="HVY61_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QMG39032.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            5955..6110
                     /gene="csl"
                     /locus_tag="HVY61_24025"
     CDS             5955..6110
                     /gene="csl"
                     /locus_tag="HVY61_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QMG39033.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGSL"
                     /name="csl"
     Typing          7..94
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          complement(6368..6914)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.171"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          749..2298
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catacaatcc gcatcgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
       61 actgggtcag ggctgcgccc cgacacccgc taaaacctgc tgacgcgccc tgacgggctt
      121 gtctgctccc ggcatccgct cacaggcaag ctgtgaccgt ctccgggagc tgcatgtgtc
      181 agaggttttc accgtcatcc ccgaaacgtg cgaggcagct gcggtaaagc tcatcggcgt
      241 ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagttcg ttgagctttt
      301 ccagcagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
      361 gtttagtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
      421 tgaaacgcga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
      481 aacgctgtga gggtaaacaa ctggcggtat ggatgcggcg ggtctgtctg ggtgagccgg
      541 ttgcccgttc cgggaaactg ccgacactgg caccgccgtt actgcgtcag ctggccgcca
      601 tcggaaataa cctgaatcag acagcccgta aggtgaacag cgggcagtgg tcttccggtg
      661 accgggttca ggtggtggcc gcactgatgg ccatcgggga cgagctgcgc cggctgcgtc
      721 tggccgtcag ggaacagggg acgcgggatg atagttaaat ttcatgccag gggaaaaggt
      781 ggtggcagtg gtccggttga ttacctgctg gggagggagc gtaaccgcga aggcgcaacg
      841 gtgcttcagg gtaatccgga agaagtccgg gaactcatcg atgccacgcc atttgcgaag
      901 aaatacacgt caggcgttct gtcgttcgcg gagaaggagc tgccgccggg aggacgtgaa
      961 aaagtgatgg cgagctttga gcgcgtactg atgcccggtc tcgaaaagaa tcagtacagc
     1021 atcctgtggg tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac
     1081 atggagctac agaccggaaa acgcctccag ccgtactacg accgggcaga caggccgaga
     1141 attgatgcct ggcagacgct ggtgaatcac cattacgggc tgcatgaccc caacgccccg
     1201 gagaaccgcc ggatactgac actccctgat aacctgccgg aaacgaaaca ggcgcttgca
     1261 gagagcgtca cacgcgggat agacgctctt tatcatgtcg gggagataag aggccgtcag
     1321 gatgtgattc aggcgctcac agaggcaggg cttgaagtgg tcagggtgac gcgaagcagt
     1381 atcagcattg cagacccgaa cggcgggaag aatatcaggc tgaaaggagc attttatgag
     1441 caatctttta cagacgggcg tggagttcga gaaaaagctg aaagagagag ccgaatctac
     1501 agagaaaatg ctgaacgacg aattcaggaa gctcggcgaa tctgtaagcg aggctgtgac
     1561 ctcaaacgag acgaaaatca aaggcgctat agcccagttt acggcctcga cggaggaatc
     1621 gctgaaaaaa caccgggaag gggtgaaaga ggcgatgatg cagcaccgga aggacatgtt
     1681 aaagctggca gggaatacgg gcatgaggtt gctggggata gtctttctcc tgtttaccgc
     1741 gagtggcggg acgctctggt atcttggcgg gcggatacag gcgaacctgg aagaaatcag
     1801 gatacaggaa gagacattgc agaaactgaa cgcgaagaca tggggcgtgg agtttgtgca
     1861 ggacgggaga aggaaatttc tggtccttcc gtacgggaaa tcggcaacgg tgattcccta
     1921 tcaggggaag gactgggtac aactgacgga gtgacacaga gtgacggagc tggaaagaca
     1981 tttgctgaac gcattagagc aactgcaaca ggactatatg cagcggctga gcgaatggga
     2041 gagcgccttc gtggaattgc agaaaatgtt ttcgcttacg caacgggaca acgcgatgct
     2101 gaacgagcag gtcatgcagt tgagtcagca ggtgcagcac ttgagcgagc ggacagaacg
     2161 cttgagccag ttatacagcg agaacaggag ataagggacg aacggctgat acaggaacgc
     2221 gaacatgcgt tatccctgga acgggagcgt cagccggaaa tacaggaacg cacgctggat
     2281 ggtccttcgc tgggatggtg aaaccatgaa aaatggcagc ttcagtggat taagaggggt
     2341 taatgtggcc tgtaccatct ggttgcatag gtattcatgc ggttaaaatt tatcaggcgc
     2401 gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc
     2461 gctgaacgcg tttcagcggt gcgtacaata agggattatg gtaaatgtct cccttactgc
     2521 ctgccctcgt agccatcgag ataaaccgca gaaatcgtgt cagccagcag cttcattttt
     2581 tccggtgatg gcatatcact gttcgtgtat tttctgcccg caaaggtggt tgtggttctc
     2641 agtgactgaa ggaattcttc agagtggaat tcttttacac gttgctgtgc cgtttctgct
     2701 gatactccgg ctgctttgtc tttcatcccc atatcccgga actgctgaaa gaacgggtgc
     2761 atcatacgta tggtgtctgc tttctgggtg tggaagttag gatcaaatcc atcatctgaa
     2821 gcataaacat aatgccgggt atgtcgttcc aggctggtct ttggtgtgca tcctgtaaga
     2881 gctattgttg ctgttatcag aggtatcaga agcagcctga cagcggcaga tctcataaac
     2941 atctccttgc gtttttaatc ttgatactca gggagttata tctccgaaga gtatctctta
     3001 tcatacgtcg gtttactccc aatgtgattt gatggtcaat gagtcaggaa taatcccgtg
     3061 aaaaaatgtg tgccagtcct gctttttagt gttctttctg cttcggttgc cagtcatgcg
     3121 gacagcaatg tttttcaaca aaaggccgag aagacaaaaa acggcattgt tgcggatgtt
     3181 aatgccagac agaaaatgtt cagtaacaga ctgaagatac aacttcagat cgatgagttg
     3241 agagcagagc tggctggcac cagagatgcc caaaaaagag aggatttgcg taaaaaactg
     3301 gcgtctctgg agcggcaaaa gaaatcctta cgctgatggg ggcgtatttt ccccggtttc
     3361 ttccataacc tttttaccgt tcagatattt acgggcgagg cgaatgcgtc ttgcctggta
     3421 taccgccgag taagcctggg tgggattcac accgttactg atttcactga ccacatgacg
     3481 ctgatcagcc agtgtcagcg tgtaaacctg tttctggatg aggtcacggg ctttgtcatg
     3541 ctcatatccg gcagcaacca gggtgtcgag aagtcgcttg tctgttttgt tcagtttatg
     3601 gcggggagat ttgctcacat cgttctccgt tttttcatga tccaacagtt ttatatctca
     3661 tcctgatttg ctccggtgtt ctgtttcgac gttctcaatg tctgcctccc catagtgtga
     3721 tccttattgc aaaaatgaaa atccatgctc ttgacatgga taatgctgag tagtaggttt
     3781 ttgctgaata caaaaccagt ggttatatgt acagtatatt gcttttaatt tattgtttta
     3841 attatcaaag aggaatttat ggctaaagaa ctaagtggat atggcccaac tgctggtgag
     3901 tctgtggggg gaacaggggc gaatctgaat cagcaaggtg gaaataataa tagtaatagt
     3961 ggggttcact ggggtggtgg ttcaggctct ggtaatggag gtggtgaaca tggaagtcag
     4021 acggggtggg gatggagtaa aaccaataat ccggatgtgc cgccttatgt tgatgataat
     4081 gggcaggtgc gcattaccat cacaaatggt ctggtaaaaa cacctgtata tggagttcct
     4141 ggggctggcg ggaatagtga tgttcagggg ggctatattc ctgaaaatcc taatgatgaa
     4201 gttgctcgta agtgggataa aaacaactta ccgcgagaga ttgatgtgag tatcgatggt
     4261 tttaaatatc gtgtaacgct taatgataat ggtagagcta tcggtattct tcgaacaggt
     4321 gtccgtcctt acgtagggag tgaaaaagct aaggcgggta ttatggaaaa aattaatcat
     4381 aaaacacctg aggagatata tgaggcgtta ggttttaata aagatgaatc tcagcggcag
     4441 gaaaaagcaa aacaacaggc tgaagatgcg tgggataatt tgcctcctaa tgttagaaaa
     4501 tttgatgttg atgttgaaca gttccactat ttagtggtgc ttgatgatta tggtaatgtt
     4561 ttatctataa ctagaacagg tgttcgtcct tatgtaggga gtgaaaaagc taaggctggt
     4621 attatggata aagtcgacca taagaccccg gaagagatat atgaggcatt aggatttaat
     4681 aatgaagagc ctcaacgaca gaatcaagct aaaaaagctg cttatgatgt gttttattct
     4741 ttcagtatga accgtgaccg gattcagagt gatgtactta ataaagctgc tgaagttatc
     4801 tctgatatag gtaataaggt cggtgattat cttggaaatg cttataagtc gttagcaaga
     4861 gaaattgctg atgatgtcaa aaatttccag gggaaaacca tccgtagcta cgatgatgca
     4921 atggcgtcat taaataaagt tttgtctaat cctgggttta aatttaacag ggccgacagt
     4981 gatgcactgg cgaacgcctg gcgaagtatt gatgctcagg atatggcaaa taaactgggt
     5041 aatatcagca aggctttcaa atttgccgat gtggtgatga aggttgagaa ggtccgggag
     5101 aaaagcatcg agggctatga aaccgggaac tggggacctc tgatgctgga ggtggagtcc
     5161 tgggtgctca gtggcattgc ttctgctgtg gccctggggg ttttttcagc cacattaggg
     5221 gcatatgcct tatctcttgg ggctcctgct attgctgtcg gtatcgtagg tattctactc
     5281 gcagcagttg taggtgcatt aattgatgac aagtttgcag atgcactaaa taacgaaatc
     5341 attaaacccg ctcattaata aataccgggt aaaaggaagg cagggttact gcctttcttt
     5401 taataattta aacgtggtaa ctggtgtgaa tagcgtgaaa tagcttataa aaaaaatgat
     5461 taagtacagg ccaatataaa atagcaatag tgttgcatca ttgttggcca ttagcctagc
     5521 tggccttcct gctgtcgtta gctcaaggtt tcggaagcag agtaaataca aaaaaacagc
     5581 acagacaaat ggacttaata agcaacttct aagtacagca tttcggtttg ttttttttat
     5641 tagttttctc gttttaaatg tcaggataaa taatatcaaa gcaagggaag gcgctgtttt
     5701 acaataaata tccataacct ttgtcattaa tgggttgtag gcggaggtta ttgatgggat
     5761 gttttcagta ctggtagcta ttgtgtgtaa gaacagattg tctggatttg tatagtaaac
     5821 ggctagtata cagagtaaag gtataactcc gattaccata aataaataca ataagttatt
     5881 ggcttttcta ttgtctatat ctattgaatg ttcatcaatc atcattgacc tataggatta
     5941 taataaaggt atatatgaaa aaaattatta tttgtgccat ttcactagga ataatgctgc
     6001 ttgcagcatg ccaagtcaac aatgtcagag atactggagg tggggctgtt tcaccatctt
     6061 ccactgttac cggagtgagt atgggaagtg gtagtagtgg tagcctgtaa aggatcttct
     6121 tgagatcctt tacagatcgt cgtagtctct tgccctgtaa acgaaaaaac cgcctgggga
     6181 ggcggtttag tcgaaggtta agtcagttgg ggactgctta accgggtagc tggcttaagt
     6241 ggagcacaga taccaaatgc tgtcctttca gtgtagccgc tataaggctt caacttcaag
     6301 agttctgcgt atctaaatcc acttttcagt taccaatggc tgctgccagt ggcgctttgt
     6361 cgtgtcttac cgggttggac tcgagacgat agttaccgga taaggcgcag cggtcgggct
     6421 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gggccgagat
     6481 accaacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6541 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttccg gggggaaacg
     6601 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6661 gatgctcgtc aggggggcgg agcctatgga aaaacgccag cggcgcggcc tttttttgtc
     6721 ctttgccttt ttactcacat gttctttccg gccttatccc ctgattctgt ggataaccgt
     6781 atttccgcct tttagtgagc cggcaccgct cgccgcagcc gaacgaccga gcgcagcgag
     6841 tcagtgagcg aggaagcgga agagtgtcgt aagttgcatt ttctccttac gcatctgtgc
     6901 ggcatttcac accgtccatg gcgcactttt
//
