LOCUS       CP057350                9653 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB29-C08 plasmid pRHB29-C08_2, complete
            sequence.
ACCESSION   CP057350
VERSION     CP057350.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148278
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 9653)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH11 T3-C08
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 154.904905608821x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:04:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,807
            CDSs (total)                      :: 4,693
            Genes (coding)                    :: 4,597
            CDSs (with protein)               :: 4,597
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 37 of 96
            Pseudo Genes (incomplete)         :: 58 of 96
            Pseudo Genes (internal stop)      :: 15 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB29-C08"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB29-C08_2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(9552..9653,1..183)
                     /locus_tag="HVY61_23900"
     CDS             join(9552..9653,1..183)
                     /locus_tag="HVY61_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39010.1"
                     /translation="MNLLIISRCAKEIAERVAYLKHCDYSQMPQHRVTLRLFADITLKA
                     LNGVIAGMLKDGKSLHECSPQEKRLVAQLKALRADLHHQLASVGCDLED"
                     /name="hypothetical protein"
     gene            187..462
                     /locus_tag="HVY61_23905"
     CDS             187..462
                     /locus_tag="HVY61_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39011.1"
                     /translation="MKQEFLTVATQSADVRPEAELNRAERIKAALIAIKAELFNMSEED
                     LLQLQQDVNAAVQKRNQVSEENKVAEAGLKVHQHHQANVPSWNDYF"
                     /name="hypothetical protein"
     gene            477..797
                     /locus_tag="HVY61_23910"
     CDS             477..797
                     /locus_tag="HVY61_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39012.1"
                     /translation="MELKDRIIQTKCESDPVFRERYLRAQVKPLAEVQDLMRRGCSDPL
                     AQEKARQAIIDAVDLLLAHPEAAHHIMNVVKAAHQLTWQQAPKSSEPLNDSDLEQQIED
                     EF"
                     /name="hypothetical protein"
     gene            892..1311
                     /locus_tag="HVY61_23915"
     CDS             892..1311
                     /locus_tag="HVY61_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39013.1"
                     /translation="MTDNLPQTPASRTFQTSKGQNGRPTKYNDRIAKQICLYVAEGMSL
                     NEISKIPGMPCRATLSVWQLEHPELRQTIEGLRFIVATDYACQALECYDDLDPASENIR
                     AELAIAKAKAATLLGMARLQDLRNLPRPIQENEEC"
                     /name="hypothetical protein"
     gene            1305..1895
                     /locus_tag="HVY61_23920"
     CDS             1305..1895
                     /locus_tag="HVY61_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39014.1"
                     /translation="MLIRDQDKGKRSRLEDGYRSLENKKFQDYTDEDLRKIGTIDVALE
                     LYTTNYEQGSQMALLEMADKINNHEVAIIDDCLCMRRIAPPLPGMIHNKAVLTDPAFAY
                     MWTGYVKVIAVHSLNYKDTFKATDRARYWRMVRDNEKRLNEDLPVGGYDAVDLQGIALA
                     NLRARPDYSDNPHADCEGMFVPEDYVPQAPEQI"
                     /name="hypothetical protein"
     gene            2255..2866
                     /locus_tag="HVY61_23925"
     CDS             2255..2866
                     /locus_tag="HVY61_23925"
                     /inference="COORDINATES: protein motif:HMM:NF012464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QMG39015.1"
                     /translation="MANIAYIRVSTVQQNTERQYHAMKDIAIDKWFEEKKSGKNEDRER
                     LKEMLEYAREGDHVYVASLDRLGRNLIGILKMVEDLRERGVVLHCLKEGYVLGAEKNAL
                     QEAFFQMMGVLAQLERNIMLERQAEAYAAAKAAGKQIGRGKSKRRQESEEFVIEALKNG
                     HQPAKIARAHKISRTSVYRIRDQAIKEGKLPENFRVKAIN"
                     /name="recombinase family protein"
     gene            3286..3648
                     /locus_tag="HVY61_23930"
     CDS             3286..3648
                     /locus_tag="HVY61_23930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39016.1"
                     /translation="MDIKPIDPRWKPRDILRVLALIKQLEAKKKEVDWRKDKRDYDNEE
                     VRKKWNSIYKTFQTKKSQKTESYQVRLKNVLWWENHAQEAHESYCRALDEFKSLKGELE
                     TILARYGLKPNLLNPD"
                     /name="hypothetical protein"
     gene            3797..4507
                     /locus_tag="HVY61_23935"
     CDS             3797..4507
                     /locus_tag="HVY61_23935"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39017.1"
                     /translation="MSIKGPMDLILRETARKSGWYNDNNQPRIRDIDARLNMPRAGFQA
                     IGTYLKGEKEPRLENLARMLDLFDVAAIMPLKDGSWLVCEPCGGADLVSENKELRARVA
                     ELEQQIAEMEVKHETLPTTENTDERVDERVDERVDERVDEKHVSRKRNPKPQLFTVADL
                     KHALIENGGTLKGQNADIAFAMALNYSDIATRRVETLQPSIPLVSRLVKAASEAGIIRV
                     TREGSQGPKTLTVL"
                     /name="hypothetical protein"
     gene            4751..5344
                     /locus_tag="HVY61_23940"
     CDS             4751..5344
                     /locus_tag="HVY61_23940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39018.1"
                     /translation="MKKSNQALTDALSLATAAAHINYSKGEKVGEWILCANVPEQKVID
                     ALKKVKFAVVDGVRWRKSDDGEYYRFQLVHGALPVQKQPPETQPETQASGKTFVKWKPV
                     GVALHALKDAGVTDNNAFIVLLEMAAVAVYNAQFKGPCFSGSIETIALMARMSRVPAQR
                     ALSVLIDTGLLKVVKKGKAGVTGRTFLLPFLLEE"
                     /name="hypothetical protein"
     gene            5347..5652
                     /locus_tag="HVY61_23945"
     CDS             5347..5652
                     /locus_tag="HVY61_23945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39019.1"
                     /translation="MSNVASLGRYRWRKESEQLEDALGWVLMRPMTHRFIWYEGDKTPW
                     LMIKTDDQPLRESHEFCQWCELDDKEVHWLMERGVPVFIAGMMTPGDTEIFNISVD"
                     /name="hypothetical protein"
     gene            7424..7774
                     /locus_tag="HVY61_23950"
     CDS             7424..7774
                     /locus_tag="HVY61_23950"
                     /inference="COORDINATES: protein motif:HMM:NF024139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QMG39020.1"
                     /translation="MYNRKQADKIFCRWWLLRPPSGEKVIPLLALISESRFPYWSAGFF
                     VGGDMKPKLLKVDQVLELLNISRGKLYKLLHGEPSFPCPIKIGGQLYFISEEIDEFISS
                     RRRVKYEQSKPD"
                     /name="helix-turn-helix domain-containing protein"
     gene            7752..8339
                     /locus_tag="HVY61_23955"
     CDS             7752..8339
                     /locus_tag="HVY61_23955"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39021.1"
                     /translation="MSNLNQIKGIEVPARVTYVEGTINPALLYEAPPINLCIQVPVDFL
                     TPRHKTREDSFIRAVMFMAAAGPLKCGGGKNESDLNYVMRLYYGRNIKTDIERSLDIPV
                     VKEMRGLHGFGGKDCYGFNTQEDVRAALNAVPSEYMTEDEKRACVQLYPSNEQLGIAPP
                     PQMGFRFNACRLTTAVMRRLNKRDRLRYRCRH"
                     /name="hypothetical protein"
     gene            8430..8720
                     /locus_tag="HVY61_23960"
     CDS             8430..8720
                     /locus_tag="HVY61_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39022.1"
                     /translation="MSFTQNSFDGQDYNKIAAQKAFKAALDKKVEQIINDIPEMLKPVI
                     AAAAKELAENVPEVLRKPDPVTHDKLDEKAIYMRMLFAVSNQLGHGANWLK"
                     /name="hypothetical protein"
     gene            8742..8984
                     /locus_tag="HVY61_23965"
     CDS             8742..8984
                     /locus_tag="HVY61_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39023.1"
                     /translation="MSKYEELAAKGAELVEKRNKASSGSERELLNKEIRAIRQAQLKAL
                     SEEVQAALKLAIPAMVEKAIKESDVVKAIEELQRS"
                     /name="hypothetical protein"
     gene            8989..9408
                     /locus_tag="HVY61_23970"
     CDS             8989..9408
                     /locus_tag="HVY61_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG39024.1"
                     /translation="MDEQIKALLNQIRILETEANNHTGNERAELFKAAGKLKNQVATLL
                     NQQGRGKAESKVPDPNEYFGANLPDGFGNYFSGDYLTPEIESYFDNLTPDMRDYFEVKV
                     PENAPDAPKQEPAKAEVFDDWEYRRKVLNALGIPC"
                     /name="hypothetical protein"
ORIGIN
        1 ttgcgcctgt tcgccgacat taccctgaag gcgctcaacg gtgtaatagc tggaatgctg
       61 aaagacggaa agagcctaca tgagtgttca ccgcaagaga aacgcctggt agctcaactg
      121 aaagcattac gcgccgatct tcaccatcag ctggcttcag tcggttgcga cctggaggat
      181 taaaatatga aacaagaatt tttaaccgtt gcgacacaat cagcagacgt tcgcccggaa
      241 gctgagctga accgcgcaga acgcatcaaa gcagccttaa tagccatcaa ggcggaatta
      301 ttcaacatgt cggaagaaga cttgctacag cttcaacagg acgtaaacgc cgccgtacag
      361 aagcgcaatc aggtcagtga agagaacaaa gtggctgagg ctggattaaa agtccatcag
      421 catcatcagg ccaatgtgcc atcgtggaac gactacttct aagaagggga aaaattatgg
      481 aactgaaaga ccgcattatt cagaccaaat gcgaaagcga tccggtattc cgtgagcgtt
      541 acctacgcgc ccaggtcaaa ccgctggctg aagttcagga cttaatgcgt cgtggttgta
      601 gtgacccatt ggcacaggag aaagcacgac aggccatcat tgatgccgtt gacctgctac
      661 ttgcccatcc agaggcagcg catcacatca tgaacgtggt gaaagcagca catcagctta
      721 cctggcaaca agcaccgaaa tcatcagagc cgctaaacga ttctgacctg gaacagcaga
      781 ttgaagacga gttttaatgc catttttctt cgaattcttc gctaaaacgg ggccaaaacg
      841 gccctataaa caacaaaaca ggagcaaaaa ttgagataaa agaggtgatt tatgactgat
      901 aatctgccgc aaacgccagc cagccggact tttcagacaa gtaaagggca aaatggacgc
      961 ccaacaaaat acaatgaccg catagcaaaa cagatttgct tatatgttgc ggaaggaatg
     1021 agcctcaacg aaatctcgaa aatccccgga atgccttgcc gggcaacgtt aagcgtctgg
     1081 cagcttgaac acccggaact gaggcagact atcgagggat taaggtttat tgtcgccacc
     1141 gattacgctt gccaggcgct ggaatgttac gacgacctcg atccagccag cgagaatatc
     1201 cgggctgaac tagccattgc caaagcgaaa gcggcaacgt tactcggcat ggcacgacta
     1261 caggatttgc gtaatctgcc gcgaccaata caggagaatg aagaatgtta atccgcgacc
     1321 aggacaaagg taaacgcagc cgccttgaag acggttatcg ctcgctggag aacaagaaat
     1381 tccaggacta caccgatgaa gacctgcgga aaatcggcac cattgacgtg gcactggaac
     1441 tgtacaccac caactacgaa caagggagcc aaatggcgct gttagaaatg gctgacaaga
     1501 tcaacaacca cgaagtcgcc atcatcgacg actgcctctg tatgcgccgc atcgcgccgc
     1561 cgctgcctgg gatgattcac aataaggcgg tactgactga cccggcgttc gcctatatgt
     1621 ggacgggata cgtgaaagtc attgctgttc atagcctgaa ctacaaagac acattcaaag
     1681 caaccgaccg cgcccggtac tggcgcatgg tccgcgacaa tgagaagcga ttgaatgaag
     1741 atctgccagt tggtggctat gacgccgttg acctccaggg tatcgcactg gctaaccttc
     1801 gtgcccgacc ggactacagc gacaaccctc acgccgattg cgaaggcatg tttgtaccgg
     1861 aagattacgt gccgcaagcg ccggagcaaa tttagttttc ggacaagtac gcgcgcgcag
     1921 ccgacgccag ggggatagac tggcggcctt caaacgcccc gattcagggc atttagccta
     1981 agtcattgat ttttcaatta tccaaatctg gataatgccg accatcacgc cacaaatgcc
     2041 ctacgagaaa aatccctcac aactaccctg ccaatttttc agatttcacc aaaacgcgcc
     2101 tgattcgata gcgtccgcaa ccatgtttga ttgaacaccg ggtttttact tgatgcgcat
     2161 caaaaaagtg ttacaaatgc cttttatcga ggggtatttg ctcttaatgt gtccgtaatg
     2221 aagaagcaag ggtatttgaa cacagggggc agtcatggca aacatcgctt acattcgcgt
     2281 gtcaactgtt caacagaaca ctgagcgcca gtatcacgca atgaaagata ttgcgatcga
     2341 caagtggttt gaagagaaga agtctggcaa gaacgaagac cgcgaacggc tgaaagaaat
     2401 gctcgagtat gccagagaag gtgaccacgt atacgttgcc agcctggacc gtttggggcg
     2461 caaccttatc gggattctga aaatggttga agacctgaga gaacgcggcg ttgttctcca
     2521 ttgcctgaaa gaaggttacg tgctgggcgc tgagaaaaac gcgttacagg aggcgttctt
     2581 ccagatgatg ggagtgttag cccaactgga gagaaacatc atgttagagc gtcaggcgga
     2641 agcgtacgca gcagcaaaag cggcgggtaa gcagattggt cggggtaaga gtaaacgtcg
     2701 ccaggagagc gaagagtttg ttatcgaggc gctcaaaaac ggtcaccagc cagcgaagat
     2761 tgcccgtgcg cacaagatca gccggacaag cgtttaccgt atccgtgacc aggctatcaa
     2821 agagggcaag ctaccggaaa acttccgcgt taaagccatc aactaaccgc aaaacaaagg
     2881 ggagcaagcg ttagcctgtt cccctttctt gtaccctctc acaatccccc gattgccatc
     2941 acattttaac gccacaccat ttcatcgttc acgtttagcc gtagaaaggc attttgtgac
     3001 acccttcaca aaaactcttt catcgagcgg ttactgccga tacaagacta atcaaattga
     3061 ttaaggtcaa tgtttatcac cgtgcgcagc gttatttata actcaacgga agggaaaccc
     3121 ttcaaacgac ggaaaccagg cgatgaaagg gaggtgtgaa atgggtattc gcaacaatgc
     3181 cggatggtgg aaacggttta aacacttcca ccctgagctg gggttagctg ccgaaaccct
     3241 gggcgtgctg atgatgatgc tggtgttatg tgtggagttt tagctatgga tataaaacca
     3301 atcgatccac gctggaaacc gagggatatc cttcgcgttc tggcactaat caaacaactg
     3361 gaagcgaaaa aaaaggaggt tgactggaga aaggataaaa gagattacga caacgaggaa
     3421 gtgagaaaaa aatggaacag catttacaaa acattccaaa ctaagaaaag ccaaaaaaca
     3481 gaaagttacc aagtccgctt aaagaatgtc ttatggtggg aaaaccacgc acaagaagcg
     3541 catgagtcat attgccgcgc gttggatgag ttcaaatctc tgaaagggga gttagaaaca
     3601 atcctcgcca ggtacggatt gaaacctaac cttttgaatc cagattgata gctttaagaa
     3661 caacgaaaac acccggcccc gcgccgggct ttttgtgatc tgcgtcatct tttagcgata
     3721 atcaccgaca aattatcttc gctagtgata gcctttaatc gagattaaat catcgttgaa
     3781 agggttggag gttgaaatga gcatcaaggg gccaatggat cttatcctga gagaaacagc
     3841 cagaaaaagc ggatggtaca acgacaacaa tcagccgcgt attcgtgaca ttgacgcaag
     3901 gctaaacatg ccgcgcgccg gattccaggc cataggcacg tatctaaaag gtgagaaaga
     3961 gccacgctta gaaaacctgg cgagaatgct ggatttgttc gatgtggcgg caattatgcc
     4021 attgaaagat ggaagctggc ttgtatgtga accatgcggc ggcgcggatc tggtaagcga
     4081 gaataaggaa ttacgcgcac gtgtcgcgga actggaacag cagatcgcag aaatggaagt
     4141 aaaacacgaa acccttccaa cgacggaaaa cactgatgaa agggtcgatg aaagggtcga
     4201 tgaaagggtc gatgaaaggg tcgatgaaaa gcacgtaagc agaaagcgca atcctaaacc
     4261 gcagctattt acggtcgctg accttaagca cgctttaatc gagaacggcg gcacgttgaa
     4321 agggcaaaac gctgatatag ctttcgcgat ggcacttaac tacagcgaca tagcaacgcg
     4381 ccgggtagaa acattacaac catctatccc tctggtgagc cgtctggtga aagcagcaag
     4441 tgaggccgga attatccgcg tgacgcgcga agggagccag ggaccgaaga cgttaacagt
     4501 cttgtaacga tgataaatca tgcgatgaaa gggaatcagc atcatttcat gaaacgcaac
     4561 gacatgcttg attgttgcga aaagtgaaat aagccgctca aatcaacagt ttgcgagagt
     4621 tttttaatag cagtatcgta gaaagcagaa agcccggcgg catccgggct atcaatgaaa
     4681 ccaacattca acaggagatt caaaccatga tgaaaaatgt tgtcagtcaa ttaacgtggg
     4741 agatagatcg atgaaaaaat caaatcaagc gttaaccgac gctttaagtc tagcaaccgc
     4801 cgccgcgcac atcaactaca gcaaaggcga aaaggttgga gaatggatac tatgcgcgaa
     4861 cgtgcctgaa caaaaagtta tagacgccct gaagaaagtt aagttcgctg tggtcgatgg
     4921 cgtgcgctgg agaaaaagcg acgatggcga atattatcgc tttcagcttg ttcacggtgc
     4981 gctgcctgta caaaaacaac cgccagagac gcaaccagag acgcaagcaa gcggtaaaac
     5041 cttcgttaag tggaagcctg ttggcgtggc acttcatgcg ctgaaagacg ctggcgtgac
     5101 cgataacaac gcttttatcg tcctacttga gatggcggcg gtagcggtat ataacgcgca
     5161 attcaaagga ccgtgcttca gtggcagcat tgagacgatc gcacttatgg cgcgcatgag
     5221 ccgagtaccc gcgcaacgcg ccttgtctgt gctgattgat actggtttgc tgaaagtagt
     5281 caaaaaggga aaagcaggtg tgaccgggcg cactttcttg ctgcctttct tgctggagga
     5341 atgattatgt cgaatgtagc atcactgggc cgataccgtt ggcgcaagga gagtgaacag
     5401 cttgaagatg cgttaggttg ggtgctaatg aggccaatga cgcatcgttt tatctggtac
     5461 gaaggcgata aaacaccgtg gttaatgatt aagacagacg atcaaccgct aagggaaagt
     5521 cacgaatttt gccaatggtg cgagctggat gacaaagaag tccactggct aatggagcgc
     5581 ggcgtaccag tttttatcgc tggcatgatg acgccaggcg acacagaaat cttcaacatc
     5641 tcggtagact gacccgctgc ggcgggtttt ttcatgccgt aaacgtcaac caatctatcc
     5701 aactaattgt ttacatgtga tccgtaccct gtggataagt gccgccagcc cagtattttg
     5761 tcattgcgta tagattctat actcaatgat tggtttccca ttgcgtatag attctatact
     5821 ttcccattgc gtatagattc tatactttcc attgcgtata gattcgatac gcatattaag
     5881 gtttttaagg aaaagaaata atgaaaagat aaggcgcggg cgcgcgtgat ggggatggat
     5941 tggatggacg tgtcttcagc atcctgcctg acgattgaga tcgagatagc tgccagttcc
     6001 cattggctta gcaggaggga ggcgcgcttc gcttgctcct gcgtcccttg ccaggcgggt
     6061 atctctgttc ggccaggtct gccctacact gggcaggcat ataaccggga gtgcggcagc
     6121 cttatatcgg cctccggcct cataaaaccc gctggaatat taactttgtg cttaagaaca
     6181 aaatggcata acagggataa aatcgattac gaatcacaaa aaatggctgt aagatattta
     6241 cagatgccaa tttttctgaa tggaaaacgg ctagatatta accagtgtcg aagcccacac
     6301 tataaatttg gcgatacgcg tcgggatagc gcgaaaacat atcgttcccc agcacatcgg
     6361 agtgccaccg ggctggtaaa aaaaatctaa cgggcggtaa cagtaaccgg gcggaagtga
     6421 tgggatccga acgcagccgc gccagaggct ggttgaaacg ggtttatgcg gttgccccca
     6481 ccgtagtgaa gggattgggg cgaggccggt cagtgcgctg gctacaggga cagaaacaga
     6541 aagcaacccg atatttccgg cgaaattact taacaggcaa tgacttcccc gcaccccatt
     6601 caaagacaga gatctgccca ctaatcccca ccagacaaac cccaacaaat tttgtccaaa
     6661 tcagcatcat cacgcatacg accaaatcaa accatcctga atagctcaaa tccttcctga
     6721 atggccccaa gaacgcgcta aactaatcgc agtagcgcaa tcgtattacc tcaccactta
     6781 atctcgttat agggccaaat ggaacgttcc cattaattcc cagtcaatct cctgatagtt
     6841 cagtggtggc tgatgcgctt gtgatacccg cgccgcacgc atccacaccg ccgccgcatc
     6901 ggcttcatat cgatattgtc cgcagtcact ggcatacgat accaaatagc ccgatctgga
     6961 aaactacctg cgatgttaca catttcagca ggtcgcaggc cgcattctac cgtctggatc
     7021 cgcatgtctg attaagcgtg atatcggacg ccatcatcgc cgcgtcttac catgcgccgg
     7081 gaataggtcc gatcgtcgtt tctgtgcttg ctcctgaggc ctaaaacgca ctgaagccat
     7141 tcagggtaag caaacctata gctgagaatt ttattaattc tgaggttatt tttccgcgtg
     7201 tatggcgcgc atccgttgat gcgatttcat taaatttttc gcattgccac aaaagattag
     7261 cgcgttccag atactattcc tttcgcgtat gggtcattca ggatgtggat ctgtggttgg
     7321 ctggcagaag acagattggg atagccctga ttagccattc aggatgagaa tcgtcatcaa
     7381 ctctggataa gcggataaac gggcgcgcga tgatagcaca agtttgtaca accgtaaaca
     7441 agctgataaa atattttgta gatggtggtt attgcgtcca ccatcgggag aaaaggtaat
     7501 tcctttatta gccttaattt cagaatcccg ctttccatat tggtcagcgg gtttttttgt
     7561 tggaggcgat atgaagccga aattgctaaa agtagatcag gttctcgagc tacttaatat
     7621 cagccgtggc aagctgtata agttgctaca cggtgaacca tcatttccat gcccaattaa
     7681 aatcggcggg cagctttatt ttatttctga agagatagac gagttcatat cgtcgcggcg
     7741 gagagtgaag tatgagcaat ctaaaccaga ttaaaggcat cgaagttccg gcccgtgtca
     7801 cttatgtcga ggggacaatt aacccggcgt tactatatga agcgccgccg attaatttat
     7861 gtatccaggt tccggttgat tttttaacac cacgccacaa gacaagggaa gatagtttca
     7921 ttcgcgctgt catgtttatg gcggcagctg gtccgcttaa atgcggcggc ggtaaaaacg
     7981 aatctgacct taattacgtc atgcgccttt attatggccg caatattaaa acggacattg
     8041 agcgctcgct tgatattccc gtagtgaaag aaatgcgcgg gttacatggt tttggtggca
     8101 aagactgtta cgggttcaat actcaggaag acgtccgcgc cgcacttaat gcggtcccat
     8161 ctgagtacat gacggaagat gagaagcgcg cttgcgttca gctgtatccc agcaatgagc
     8221 agctaggcat agcaccgccg ccgcagatgg gtttccggtt caacgcctgc cgtctgacta
     8281 ccgcagtgat gcgcagatta aacaaacgcg atcgccttcg ctacaggtgt cgacattaaa
     8341 aaatcgggaa caactatggc caacaaaatc tattttttgc gtagcaacac cattctatag
     8401 aaagctaatt aattaaagag gaaaaaatca tgtcatttac tcagaatagc tttgacgggc
     8461 aagattacaa caaaattgcg gcacagaaag catttaaagc cgcgctggat aagaaggttg
     8521 aacaaattat taacgatatc ccggaaatgc ttaaacctgt tattgctgcg gctgctaaag
     8581 aacttgccga aaatgttccg gaagtactgc gtaaacccga cccagtcacg cacgataagc
     8641 tggacgaaaa ggcaatatat atgcggatgc tattcgctgt cagcaatcag ttagggcacg
     8701 gagctaattg gcttaaataa cgatgaacgg gagacaatat tatgagcaaa tatgaagagt
     8761 tggcagcaaa aggcgctgaa ttggtggaga agcgaaataa agccagcagc ggcagcgagc
     8821 gtgagctgct aaataaagaa atccgggcaa ttcgtcaggc gcaattaaaa gcattgtcag
     8881 aagaagtcca ggcagcttta aaactggcaa ttcctgcgat ggttgagaaa gcgattaaag
     8941 aaagcgatgt agtaaaagca attgaagaat tacagcggag ttaatgagat ggacgaacaa
     9001 atcaaggcgc tgttaaacca gatccgcata ttagaaacag aagcgaacaa ccatacaggt
     9061 aatgaacgtg cggagctgtt taaggcagca ggtaaattaa aaaaccaggt ggcaacgcta
     9121 ttaaaccagc aggggagagg taaagcagaa agcaaagtgc ccgacccgaa cgaatacttc
     9181 ggcgctaatt tgccagatgg atttggtaat tacttctctg gtgattatct cactccggaa
     9241 attgaatcct atttcgataa tctgacgcca gatatgcgcg actactttga agttaaggtg
     9301 ccggaaaacg cgccagatgc gcctaaacag gagccagcga aggcagaagt cttcgacgac
     9361 tgggaatatc gccgcaaggt gctcaatgcg ctgggtatcc cgtgctaatg gcaagaaaaa
     9421 gccaggtatt gcgcctggct caatctcaac aatgcgacca atgaacaaat cgatatgaat
     9481 ggtgatgctc gaagtaatgt tcccttctat tgtatcaacg gtcgcatgaa tcgacagaaa
     9541 tgaggtgaga gatgaattta ttaatcatca gtcgttgcgc caaagagatc gccgagcgcg
     9601 tcgcctatct gaagcactgc gactatagcc aaatgccaca gcaccgcgtc acc
//
