LOCUS       CP057338                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB29-C22 plasmid pRHB29-C22_3, complete
            sequence.
ACCESSION   CP057338
VERSION     CP057338.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148284
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH11 T3-C22
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138.27439744754x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:00:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,806
            CDSs (total)                      :: 4,692
            Genes (coding)                    :: 4,596
            CDSs (with protein)               :: 4,596
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 37 of 96
            Pseudo Genes (incomplete)         :: 58 of 96
            Pseudo Genes (internal stop)      :: 15 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB29-C22"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB29-C22_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(20..211)
                     /locus_tag="HVY67_23970"
     CDS             complement(20..211)
                     /locus_tag="HVY67_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMG10746.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            276..599
                     /locus_tag="HVY67_23975"
     CDS             276..599
                     /locus_tag="HVY67_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMG10755.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            757..1299
                     /locus_tag="HVY67_23980"
                     /pseudo=""
     CDS             757..1299
                     /locus_tag="HVY67_23980"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1277..1795
                     /locus_tag="HVY67_23985"
     CDS             1277..1795
                     /locus_tag="HVY67_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMG10747.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            1802..2035
                     /gene="mbeD"
                     /locus_tag="HVY67_23990"
     CDS             1802..2035
                     /gene="mbeD"
                     /locus_tag="HVY67_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMG10748.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(2355..2777)
                     /gene="exc2"
                     /locus_tag="HVY67_23995"
     CDS             complement(2355..2777)
                     /gene="exc2"
                     /locus_tag="HVY67_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMG10749.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            2899..3177
                     /locus_tag="HVY67_24000"
     CDS             2899..3177
                     /locus_tag="HVY67_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10750.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(3169..3459)
                     /locus_tag="HVY67_24005"
     CDS             complement(3169..3459)
                     /locus_tag="HVY67_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10751.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            3700..5199
                     /gene="csa"
                     /locus_tag="HVY67_24010"
     CDS             3700..5199
                     /gene="csa"
                     /locus_tag="HVY67_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QMG10752.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            complement(5227..5766)
                     /gene="csi"
                     /locus_tag="HVY67_24015"
     CDS             complement(5227..5766)
                     /gene="csi"
                     /locus_tag="HVY67_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QMG10753.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            5796..5951
                     /gene="csl"
                     /locus_tag="HVY67_24020"
     CDS             5796..5951
                     /gene="csl"
                     /locus_tag="HVY67_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QMG10754.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGSL"
                     /name="csl"
     Typing          6778..6865
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          complement(6209..6755)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.171"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          590..2139
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc cccgaaacgt gcgaggcagc
       61 tgcggtaaag ctcatcggcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg
      121 cgtccagttc gttgagcttt tccagcagcg ttaatgtctg gcttctgata aagcgggcca
      181 tgttaagggc ggttttttcc tgtttagtca ctgatgcctc cgtgtaaggg ggatttctgt
      241 tcatgggggt aatgataccg atgaaacgcg agaggatgct cacgatacgg gttactgatg
      301 atgaacatgc ccggttactg gaacgctgtg agggtaaaca actggcggta tggatgcggc
      361 gggtctgtct gggtgagccg gttgcccgtt ccgggaaact gccgacactg gcaccgccgt
      421 tactgcgtca gctggccgcc atcggaaata acctgaatca gacagcccgt aaggtgaaca
      481 gcgggcagtg gtcttccggt gaccgggttc aggtggtggc cgcactgatg gccatcgggg
      541 acgagctgcg ccggctgcgt ctggccgtca gggaacaggg gacgcgggat gatagttaaa
      601 tttcatgcca ggggaaaagg tggtggcagt ggtccggttg attacctgct ggggagggag
      661 cgtaaccgcg aaggcgcaac ggtgcttcag ggtaatccgg aagaagtccg ggaactcatc
      721 gatgccacgc catttgcgaa gaaatacacg tcaggcgttc tgtcgttcgc ggagaaggag
      781 ctgccgccgg gaggacgtga aaaagtgatg gcgagctttg agcgcgtact gatgcccggt
      841 ctcgaaaaga atcagtacag catcctgtgg gtggagcacc aggacaaggg acggcttgag
      901 ctgaattttg tcattccgaa catggagcta cagaccggaa aacgcctcca gccgtactac
      961 gaccgggcag acaggccgag aattgatgcc tggcagacgc tggtgaatca ccattacggg
     1021 ctgcatgacc ccaacgcccc ggagaaccgc cggatactga cactccctga taacctgccg
     1081 gaaacgaaac aggcgcttgc agagagcgtc acacgcggga tagacgctct ttatcatgtc
     1141 ggggagataa gaggccgtca ggatgtgatt caggcgctca cagaggcagg gcttgaagtg
     1201 gtcagggtga cgcgaagcag tatcagcatt gcagacccga acggcgggaa gaatatcagg
     1261 ctgaaaggag cattttatga gcaatctttt acagacgggc gtggagttcg agaaaaagct
     1321 gaaagagaga gccgaatcta cagagaaaat gctgaacgac gaattcagga agctcggcga
     1381 atctgtaagc gaggctgtga cctcaaacga gacgaaaatc aaaggcgcta tagcccagtt
     1441 tacggcctcg acggaggaat cgctgaaaaa acaccgggaa ggggtgaaag aggcgatgat
     1501 gcagcaccgg aaggacatgt taaagctggc agggaatacg ggcatgaggt tgctggggat
     1561 agtctttctc ctgtttaccg cgagtggcgg gacgctctgg tatcttggcg ggcggataca
     1621 ggcgaacctg gaagaaatca ggatacagga agagacattg cagaaactga acgcgaagac
     1681 atggggcgtg gagtttgtgc aggacgggag aaggaaattt ctggtccttc cgtacgggaa
     1741 atcggcaacg gtgattccct atcaggggaa ggactgggta caactgacgg agtgacacag
     1801 agtgacggag ctggaaagac atttgctgaa cgcattagag caactgcaac aggactatat
     1861 gcagcggctg agcgaatggg agagcgcctt cgtggaattg cagaaaatgt tttcgcttac
     1921 gcaacgggac aacgcgatgc tgaacgagca ggtcatgcag ttgagtcagc aggtgcagca
     1981 cttgagcgag cggacagaac gcttgagcca gttatacagc gagaacagga gataagggac
     2041 gaacggctga tacaggaacg cgaacatgcg ttatccctgg aacgggagcg tcagccggaa
     2101 atacaggaac gcacgctgga tggtccttcg ctgggatggt gaaaccatga aaaatggcag
     2161 cttcagtgga ttaagagggg ttaatgtggc ctgtaccatc tggttgcata ggtattcatg
     2221 cggttaaaat ttatcaggcg cgatcgcggc agtttttcgg gtggtttgtt gccattttta
     2281 cctgtctgct gccgtgatcg cgctgaacgc gtttcagcgg tgcgtacaat aagggattat
     2341 ggtaaatgtc tcccttactg cctgccctcg tagccatcga gataaaccgc agaaatcgtg
     2401 tcagccagca gcttcatttt ttccggtgat ggcatatcac tgttcgtgta ttttctgccc
     2461 gcaaaggtgg ttgtggttct cagtgactga aggaattctt cagagtggaa ttcttttaca
     2521 cgttgctgtg ccgtttctgc tgatactccg gctgctttgt ctttcatccc catatcccgg
     2581 aactgctgaa agaacgggtg catcatacgt atggtgtctg ctttctgggt gtggaagtta
     2641 ggatcaaatc catcatctga agcataaaca taatgccggg tatgtcgttc caggctggtc
     2701 tttggtgtgc atcctgtaag agctattgtt gctgttatca gaggtatcag aagcagcctg
     2761 acagcggcag atctcataaa catctccttg cgtttttaat cttgatactc agggagttat
     2821 atctccgaag agtatctctt atcatacgtc ggtttactcc caatgtgatt tgatggtcaa
     2881 tgagtcagga ataatcccgt gaaaaaatgt gtgccagtcc tgctttttag tgttctttct
     2941 gcttcggttg ccagtcatgc ggacagcaat gtttttcaac aaaaggccga gaagacaaaa
     3001 aacggcattg ttgcggatgt taatgccaga cagaaaatgt tcagtaacag actgaagata
     3061 caacttcaga tcgatgagtt gagagcagag ctggctggca ccagagatgc ccaaaaaaga
     3121 gaggatttgc gtaaaaaact ggcgtctctg gagcggcaaa agaaatcctt acgctgatgg
     3181 gggcgtattt tccccggttt cttccataac ctttttaccg ttcagatatt tacgggcgag
     3241 gcgaatgcgt cttgcctggt ataccgccga gtaagcctgg gtgggattca caccgttact
     3301 gatttcactg accacatgac gctgatcagc cagtgtcagc gtgtaaacct gtttctggat
     3361 gaggtcacgg gctttgtcat gctcatatcc ggcagcaacc agggtgtcga gaagtcgctt
     3421 gtctgttttg ttcagtttat ggcggggaga tttgctcaca tcgttctccg ttttttcatg
     3481 atccaacagt tttatatctc atcctgattt gctccggtgt tctgtttcga cgttctcaat
     3541 gtctgcctcc ccatagtgtg atccttattg caaaaatgaa aatccatgct cttgacatgg
     3601 ataatgctga gtagtaggtt tttgctgaat acaaaaccag tggttatatg tacagtatat
     3661 tgcttttaat ttattgtttt aattatcaaa gaggaattta tggctaaaga actaagtgga
     3721 tatggcccaa ctgctggtga gtctgtgggg ggaacagggg cgaatctgaa tcagcaaggt
     3781 ggaaataata atagtaatag tggggttcac tggggtggtg gttcaggctc tggtaatgga
     3841 ggtggtgaac atggaagtca gacggggtgg ggatggagta aaaccaataa tccggatgtg
     3901 ccgccttatg ttgatgataa tgggcaggtg cgcattacca tcacaaatgg tctggtaaaa
     3961 acacctgtat atggagttcc tggggctggc gggaatagtg atgttcaggg gggctatatt
     4021 cctgaaaatc ctaatgatga agttgctcgt aagtgggata aaaacaactt accgcgagag
     4081 attgatgtga gtatcgatgg ttttaaatat cgtgtaacgc ttaatgataa tggtagagct
     4141 atcggtattc ttcgaacagg tgtccgtcct tacgtaggga gtgaaaaagc taaggcgggt
     4201 attatggaaa aaattaatca taaaacacct gaggagatat atgaggcgtt aggttttaat
     4261 aaagatgaat ctcagcggca ggaaaaagca aaacaacagg ctgaagatgc gtgggataat
     4321 ttgcctccta atgttagaaa atttgatgtt gatgttgaac agttccacta tttagtggtg
     4381 cttgatgatt atggtaatgt tttatctata actagaacag gtgttcgtcc ttatgtaggg
     4441 agtgaaaaag ctaaggctgg tattatggat aaagtcgacc ataagacccc ggaagagata
     4501 tatgaggcat taggatttaa taatgaagag cctcaacgac agaatcaagc taaaaaagct
     4561 gcttatgatg tgttttattc tttcagtatg aaccgtgacc ggattcagag tgatgtactt
     4621 aataaagctg ctgaagttat ctctgatata ggtaataagg tcggtgatta tcttggaaat
     4681 gcttataagt cgttagcaag agaaattgct gatgatgtca aaaatttcca ggggaaaacc
     4741 atccgtagct acgatgatgc aatggcgtca ttaaataaag ttttgtctaa tcctgggttt
     4801 aaatttaaca gggccgacag tgatgcactg gcgaacgcct ggcgaagtat tgatgctcag
     4861 gatatggcaa ataaactggg taatatcagc aaggctttca aatttgccga tgtggtgatg
     4921 aaggttgaga aggtccggga gaaaagcatc gagggctatg aaaccgggaa ctggggacct
     4981 ctgatgctgg aggtggagtc ctgggtgctc agtggcattg cttctgctgt ggccctgggg
     5041 gttttttcag ccacattagg ggcatatgcc ttatctcttg gggctcctgc tattgctgtc
     5101 ggtatcgtag gtattctact cgcagcagtt gtaggtgcat taattgatga caagtttgca
     5161 gatgcactaa ataacgaaat cattaaaccc gctcattaat aaataccggg taaaaggaag
     5221 gcagggttac tgcctttctt ttaataattt aaacgtggta actggtgtga atagcgtgaa
     5281 atagcttata aaaaaaatga ttaagtacag gccaatataa aatagcaata gtgttgcatc
     5341 attgttggcc attagcctag ctggccttcc tgctgtcgtt agctcaaggt ttcggaagca
     5401 gagtaaatac aaaaaaacag cacagacaaa tggacttaat aagcaacttc taagtacagc
     5461 atttcggttt gtttttttta ttagttttct cgttttaaat gtcaggataa ataatatcaa
     5521 agcaagggaa ggcgctgttt tacaataaat atccataacc tttgtcatta atgggttgta
     5581 ggcggaggtt attgatggga tgttttcagt actggtagct attgtgtgta agaacagatt
     5641 gtctggattt gtatagtaaa cggctagtat acagagtaaa ggtataactc cgattaccat
     5701 aaataaatac aataagttat tggcttttct attgtctata tctattgaat gttcatcaat
     5761 catcattgac ctataggatt ataataaagg tatatatgaa aaaaattatt atttgtgcca
     5821 tttcactagg aataatgctg cttgcagcat gccaagtcaa caatgtcaga gatactggag
     5881 gtggggctgt ttcaccatct tccactgtta ccggagtgag tatgggaagt ggtagtagtg
     5941 gtagcctgta aaggatcttc ttgagatcct ttacagatcg tcgtagtctc ttgccctgta
     6001 aacgaaaaaa ccgcctgggg aggcggttta gtcgaaggtt aagtcagttg gggactgctt
     6061 aaccgggtag ctggcttaag tggagcacag ataccaaatg ctgtcctttc agtgtagccg
     6121 ctataaggct tcaacttcaa gagttctgcg tatctaaatc cacttttcag ttaccaatgg
     6181 ctgctgccag tggcgctttg tcgtgtctta ccgggttgga ctcgagacga tagttaccgg
     6241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6301 cgacctacac cgggccgaga taccaacagc gtgagctatg agaaagcgcc acgcttcccg
     6361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6421 gggagcttcc ggggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6541 gcggcgcggc ctttttttgt cctttgcctt tttactcaca tgttctttcc ggccttatcc
     6601 cctgattctg tggataaccg tatttccgcc ttttagtgag ccggcaccgc tcgccgcagc
     6661 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagtgtcg taagttgcat
     6721 tttctcctta cgcatctgtg cggcatttca caccgtccat ggcgcacttt tcatacaatc
     6781 cgcatcgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     6841 gggctgcgcc ccgacacccg ctaaaacctg ctgacgcgcc ctgacgggct tgtctgctcc
     6901 cggcatccgc tcacaggcaa gctgtgaccg
//
