LOCUS       CP057337                9653 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB29-C22 plasmid pRHB29-C22_2, complete
            sequence.
ACCESSION   CP057337
VERSION     CP057337.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148284
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 9653)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH11 T3-C22
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138.27439744754x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 08:00:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,806
            CDSs (total)                      :: 4,692
            Genes (coding)                    :: 4,596
            CDSs (with protein)               :: 4,596
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 37 of 96
            Pseudo Genes (incomplete)         :: 58 of 96
            Pseudo Genes (internal stop)      :: 15 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB29-C22"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB29-C22_2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(9482..9653,1..422)
                     /locus_tag="HVY67_23895"
     CDS             join(9482..9653,1..422)
                     /locus_tag="HVY67_23895"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10745.1"
                     /translation="MKKSNQALTDALSLATAAAHINYSKGEKVGEWILCANVPEQKVID
                     ALKKVKFAVVDGVRWRKSDDGEYYRFQLVHGALPVQKQPPETQPETQASGKTFVKWKPV
                     GVALHALKDAGVTDNNAFIVLLEMAAVAVYNAQFKGPCFSGSIETIALMARMSRVPAQR
                     ALSVLIDTGLLKVVKKGKAGVTGRTFLLPFLLEE"
                     /name="hypothetical protein"
     gene            425..730
                     /locus_tag="HVY67_23900"
     CDS             425..730
                     /locus_tag="HVY67_23900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10731.1"
                     /translation="MSNVASLGRYRWRKESEQLEDALGWVLMRPMTHRFIWYEGDKTPW
                     LMIKTDDQPLRESHEFCQWCELDDKEVHWLMERGVPVFIAGMMTPGDTEIFNISVD"
                     /name="hypothetical protein"
     gene            2502..2852
                     /locus_tag="HVY67_23905"
     CDS             2502..2852
                     /locus_tag="HVY67_23905"
                     /inference="COORDINATES: protein motif:HMM:NF024139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QMG10732.1"
                     /translation="MYNRKQADKIFCRWWLLRPPSGEKVIPLLALISESRFPYWSAGFF
                     VGGDMKPKLLKVDQVLELLNISRGKLYKLLHGEPSFPCPIKIGGQLYFISEEIDEFISS
                     RRRVKYEQSKPD"
                     /name="helix-turn-helix domain-containing protein"
     gene            2830..3417
                     /locus_tag="HVY67_23910"
     CDS             2830..3417
                     /locus_tag="HVY67_23910"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10733.1"
                     /translation="MSNLNQIKGIEVPARVTYVEGTINPALLYEAPPINLCIQVPVDFL
                     TPRHKTREDSFIRAVMFMAAAGPLKCGGGKNESDLNYVMRLYYGRNIKTDIERSLDIPV
                     VKEMRGLHGFGGKDCYGFNTQEDVRAALNAVPSEYMTEDEKRACVQLYPSNEQLGIAPP
                     PQMGFRFNACRLTTAVMRRLNKRDRLRYRCRH"
                     /name="hypothetical protein"
     gene            3508..3798
                     /locus_tag="HVY67_23915"
     CDS             3508..3798
                     /locus_tag="HVY67_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10734.1"
                     /translation="MSFTQNSFDGQDYNKIAAQKAFKAALDKKVEQIINDIPEMLKPVI
                     AAAAKELAENVPEVLRKPDPVTHDKLDEKAIYMRMLFAVSNQLGHGANWLK"
                     /name="hypothetical protein"
     gene            3820..4062
                     /locus_tag="HVY67_23920"
     CDS             3820..4062
                     /locus_tag="HVY67_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10735.1"
                     /translation="MSKYEELAAKGAELVEKRNKASSGSERELLNKEIRAIRQAQLKAL
                     SEEVQAALKLAIPAMVEKAIKESDVVKAIEELQRS"
                     /name="hypothetical protein"
     gene            4067..4486
                     /locus_tag="HVY67_23925"
     CDS             4067..4486
                     /locus_tag="HVY67_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10736.1"
                     /translation="MDEQIKALLNQIRILETEANNHTGNERAELFKAAGKLKNQVATLL
                     NQQGRGKAESKVPDPNEYFGANLPDGFGNYFSGDYLTPEIESYFDNLTPDMRDYFEVKV
                     PENAPDAPKQEPAKAEVFDDWEYRRKVLNALGIPC"
                     /name="hypothetical protein"
     gene            4630..4914
                     /locus_tag="HVY67_23930"
     CDS             4630..4914
                     /locus_tag="HVY67_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10737.1"
                     /translation="MNLLIISRCAKEIAERVAYLKHCDYSQMPQHRVTLRLFADITLKA
                     LNGVIAGMLKDGKSLHECSPQEKRLVAQLKALRADLHHQLASVGCDLED"
                     /name="hypothetical protein"
     gene            4918..5193
                     /locus_tag="HVY67_23935"
     CDS             4918..5193
                     /locus_tag="HVY67_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10738.1"
                     /translation="MKQEFLTVATQSADVRPEAELNRAERIKAALIAIKAELFNMSEED
                     LLQLQQDVNAAVQKRNQVSEENKVAEAGLKVHQHHQANVPSWNDYF"
                     /name="hypothetical protein"
     gene            5208..5528
                     /locus_tag="HVY67_23940"
     CDS             5208..5528
                     /locus_tag="HVY67_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10739.1"
                     /translation="MELKDRIIQTKCESDPVFRERYLRAQVKPLAEVQDLMRRGCSDPL
                     AQEKARQAIIDAVDLLLAHPEAAHHIMNVVKAAHQLTWQQAPKSSEPLNDSDLEQQIED
                     EF"
                     /name="hypothetical protein"
     gene            5623..6042
                     /locus_tag="HVY67_23945"
     CDS             5623..6042
                     /locus_tag="HVY67_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10740.1"
                     /translation="MTDNLPQTPASRTFQTSKGQNGRPTKYNDRIAKQICLYVAEGMSL
                     NEISKIPGMPCRATLSVWQLEHPELRQTIEGLRFIVATDYACQALECYDDLDPASENIR
                     AELAIAKAKAATLLGMARLQDLRNLPRPIQENEEC"
                     /name="hypothetical protein"
     gene            6036..6626
                     /locus_tag="HVY67_23950"
     CDS             6036..6626
                     /locus_tag="HVY67_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10741.1"
                     /translation="MLIRDQDKGKRSRLEDGYRSLENKKFQDYTDEDLRKIGTIDVALE
                     LYTTNYEQGSQMALLEMADKINNHEVAIIDDCLCMRRIAPPLPGMIHNKAVLTDPAFAY
                     MWTGYVKVIAVHSLNYKDTFKATDRARYWRMVRDNEKRLNEDLPVGGYDAVDLQGIALA
                     NLRARPDYSDNPHADCEGMFVPEDYVPQAPEQI"
                     /name="hypothetical protein"
     gene            6986..7597
                     /locus_tag="HVY67_23955"
     CDS             6986..7597
                     /locus_tag="HVY67_23955"
                     /inference="COORDINATES: protein motif:HMM:NF012464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QMG10742.1"
                     /translation="MANIAYIRVSTVQQNTERQYHAMKDIAIDKWFEEKKSGKNEDRER
                     LKEMLEYAREGDHVYVASLDRLGRNLIGILKMVEDLRERGVVLHCLKEGYVLGAEKNAL
                     QEAFFQMMGVLAQLERNIMLERQAEAYAAAKAAGKQIGRGKSKRRQESEEFVIEALKNG
                     HQPAKIARAHKISRTSVYRIRDQAIKEGKLPENFRVKAIN"
                     /name="recombinase family protein"
     gene            8017..8379
                     /locus_tag="HVY67_23960"
     CDS             8017..8379
                     /locus_tag="HVY67_23960"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10743.1"
                     /translation="MDIKPIDPRWKPRDILRVLALIKQLEAKKKEVDWRKDKRDYDNEE
                     VRKKWNSIYKTFQTKKSQKTESYQVRLKNVLWWENHAQEAHESYCRALDEFKSLKGELE
                     TILARYGLKPNLLNPD"
                     /name="hypothetical protein"
     gene            8528..9238
                     /locus_tag="HVY67_23965"
     CDS             8528..9238
                     /locus_tag="HVY67_23965"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMG10744.1"
                     /translation="MSIKGPMDLILRETARKSGWYNDNNQPRIRDIDARLNMPRAGFQA
                     IGTYLKGEKEPRLENLARMLDLFDVAAIMPLKDGSWLVCEPCGGADLVSENKELRARVA
                     ELEQQIAEMEVKHETLPTTENTDERVDERVDERVDERVDEKHVSRKRNPKPQLFTVADL
                     KHALIENGGTLKGQNADIAFAMALNYSDIATRRVETLQPSIPLVSRLVKAASEAGIIRV
                     TREGSQGPKTLTVL"
                     /name="hypothetical protein"
ORIGIN
        1 tgcgctggag aaaaagcgac gatggcgaat attatcgctt tcagcttgtt cacggtgcgc
       61 tgcctgtaca aaaacaaccg ccagagacgc aaccagagac gcaagcaagc ggtaaaacct
      121 tcgttaagtg gaagcctgtt ggcgtggcac ttcatgcgct gaaagacgct ggcgtgaccg
      181 ataacaacgc ttttatcgtc ctacttgaga tggcggcggt agcggtatat aacgcgcaat
      241 tcaaaggacc gtgcttcagt ggcagcattg agacgatcgc acttatggcg cgcatgagcc
      301 gagtacccgc gcaacgcgcc ttgtctgtgc tgattgatac tggtttgctg aaagtagtca
      361 aaaagggaaa agcaggtgtg accgggcgca ctttcttgct gcctttcttg ctggaggaat
      421 gattatgtcg aatgtagcat cactgggccg ataccgttgg cgcaaggaga gtgaacagct
      481 tgaagatgcg ttaggttggg tgctaatgag gccaatgacg catcgtttta tctggtacga
      541 aggcgataaa acaccgtggt taatgattaa gacagacgat caaccgctaa gggaaagtca
      601 cgaattttgc caatggtgcg agctggatga caaagaagtc cactggctaa tggagcgcgg
      661 cgtaccagtt tttatcgctg gcatgatgac gccaggcgac acagaaatct tcaacatctc
      721 ggtagactga cccgctgcgg cgggtttttt catgccgtaa acgtcaacca atctatccaa
      781 ctaattgttt acatgtgatc cgtaccctgt ggataagtgc cgccagccca gtattttgtc
      841 attgcgtata gattctatac tcaatgattg gtttcccatt gcgtatagat tctatacttt
      901 cccattgcgt atagattcta tactttccat tgcgtataga ttcgatacgc atattaaggt
      961 ttttaaggaa aagaaataat gaaaagataa ggcgcgggcg cgcgtgatgg ggatggattg
     1021 gatggacgtg tcttcagcat cctgcctgac gattgagatc gagatagctg ccagttccca
     1081 ttggcttagc aggagggagg cgcgcttcgc ttgctcctgc gtcccttgcc aggcgggtat
     1141 ctctgttcgg ccaggtctgc cctacactgg gcaggcatat aaccgggagt gcggcagcct
     1201 tatatcggcc tccggcctca taaaacccgc tggaatatta actttgtgct taagaacaaa
     1261 atggcataac agggataaaa tcgattacga atcacaaaaa atggctgtaa gatatttaca
     1321 gatgccaatt tttctgaatg gaaaacggct agatattaac cagtgtcgaa gcccacacta
     1381 taaatttggc gatacgcgtc gggatagcgc gaaaacatat cgttccccag cacatcggag
     1441 tgccaccggg ctggtaaaaa aaatctaacg ggcggtaaca gtaaccgggc ggaagtgatg
     1501 ggatccgaac gcagccgcgc cagaggctgg ttgaaacggg tttatgcggt tgcccccacc
     1561 gtagtgaagg gattggggcg aggccggtca gtgcgctggc tacagggaca gaaacagaaa
     1621 gcaacccgat atttccggcg aaattactta acaggcaatg acttccccgc accccattca
     1681 aagacagaga tctgcccact aatccccacc agacaaaccc caacaaattt tgtccaaatc
     1741 agcatcatca cgcatacgac caaatcaaac catcctgaat agctcaaatc cttcctgaat
     1801 ggccccaaga acgcgctaaa ctaatcgcag tagcgcaatc gtattacctc accacttaat
     1861 ctcgttatag ggccaaatgg aacgttccca ttaattccca gtcaatctcc tgatagttca
     1921 gtggtggctg atgcgcttgt gatacccgcg ccgcacgcat ccacaccgcc gccgcatcgg
     1981 cttcatatcg atattgtccg cagtcactgg catacgatac caaatagccc gatctggaaa
     2041 actacctgcg atgttacaca tttcagcagg tcgcaggccg cattctaccg tctggatccg
     2101 catgtctgat taagcgtgat atcggacgcc atcatcgccg cgtcttacca tgcgccggga
     2161 ataggtccga tcgtcgtttc tgtgcttgct cctgaggcct aaaacgcact gaagccattc
     2221 agggtaagca aacctatagc tgagaatttt attaattctg aggttatttt tccgcgtgta
     2281 tggcgcgcat ccgttgatgc gatttcatta aatttttcgc attgccacaa aagattagcg
     2341 cgttccagat actattcctt tcgcgtatgg gtcattcagg atgtggatct gtggttggct
     2401 ggcagaagac agattgggat agccctgatt agccattcag gatgagaatc gtcatcaact
     2461 ctggataagc ggataaacgg gcgcgcgatg atagcacaag tttgtacaac cgtaaacaag
     2521 ctgataaaat attttgtaga tggtggttat tgcgtccacc atcgggagaa aaggtaattc
     2581 ctttattagc cttaatttca gaatcccgct ttccatattg gtcagcgggt ttttttgttg
     2641 gaggcgatat gaagccgaaa ttgctaaaag tagatcaggt tctcgagcta cttaatatca
     2701 gccgtggcaa gctgtataag ttgctacacg gtgaaccatc atttccatgc ccaattaaaa
     2761 tcggcgggca gctttatttt atttctgaag agatagacga gttcatatcg tcgcggcgga
     2821 gagtgaagta tgagcaatct aaaccagatt aaaggcatcg aagttccggc ccgtgtcact
     2881 tatgtcgagg ggacaattaa cccggcgtta ctatatgaag cgccgccgat taatttatgt
     2941 atccaggttc cggttgattt tttaacacca cgccacaaga caagggaaga tagtttcatt
     3001 cgcgctgtca tgtttatggc ggcagctggt ccgcttaaat gcggcggcgg taaaaacgaa
     3061 tctgacctta attacgtcat gcgcctttat tatggccgca atattaaaac ggacattgag
     3121 cgctcgcttg atattcccgt agtgaaagaa atgcgcgggt tacatggttt tggtggcaaa
     3181 gactgttacg ggttcaatac tcaggaagac gtccgcgccg cacttaatgc ggtcccatct
     3241 gagtacatga cggaagatga gaagcgcgct tgcgttcagc tgtatcccag caatgagcag
     3301 ctaggcatag caccgccgcc gcagatgggt ttccggttca acgcctgccg tctgactacc
     3361 gcagtgatgc gcagattaaa caaacgcgat cgccttcgct acaggtgtcg acattaaaaa
     3421 atcgggaaca actatggcca acaaaatcta ttttttgcgt agcaacacca ttctatagaa
     3481 agctaattaa ttaaagagga aaaaatcatg tcatttactc agaatagctt tgacgggcaa
     3541 gattacaaca aaattgcggc acagaaagca tttaaagccg cgctggataa gaaggttgaa
     3601 caaattatta acgatatccc ggaaatgctt aaacctgtta ttgctgcggc tgctaaagaa
     3661 cttgccgaaa atgttccgga agtactgcgt aaacccgacc cagtcacgca cgataagctg
     3721 gacgaaaagg caatatatat gcggatgcta ttcgctgtca gcaatcagtt agggcacgga
     3781 gctaattggc ttaaataacg atgaacggga gacaatatta tgagcaaata tgaagagttg
     3841 gcagcaaaag gcgctgaatt ggtggagaag cgaaataaag ccagcagcgg cagcgagcgt
     3901 gagctgctaa ataaagaaat ccgggcaatt cgtcaggcgc aattaaaagc attgtcagaa
     3961 gaagtccagg cagctttaaa actggcaatt cctgcgatgg ttgagaaagc gattaaagaa
     4021 agcgatgtag taaaagcaat tgaagaatta cagcggagtt aatgagatgg acgaacaaat
     4081 caaggcgctg ttaaaccaga tccgcatatt agaaacagaa gcgaacaacc atacaggtaa
     4141 tgaacgtgcg gagctgttta aggcagcagg taaattaaaa aaccaggtgg caacgctatt
     4201 aaaccagcag gggagaggta aagcagaaag caaagtgccc gacccgaacg aatacttcgg
     4261 cgctaatttg ccagatggat ttggtaatta cttctctggt gattatctca ctccggaaat
     4321 tgaatcctat ttcgataatc tgacgccaga tatgcgcgac tactttgaag ttaaggtgcc
     4381 ggaaaacgcg ccagatgcgc ctaaacagga gccagcgaag gcagaagtct tcgacgactg
     4441 ggaatatcgc cgcaaggtgc tcaatgcgct gggtatcccg tgctaatggc aagaaaaagc
     4501 caggtattgc gcctggctca atctcaacaa tgcgaccaat gaacaaatcg atatgaatgg
     4561 tgatgctcga agtaatgttc ccttctattg tatcaacggt cgcatgaatc gacagaaatg
     4621 aggtgagaga tgaatttatt aatcatcagt cgttgcgcca aagagatcgc cgagcgcgtc
     4681 gcctatctga agcactgcga ctatagccaa atgccacagc accgcgtcac cttgcgcctg
     4741 ttcgccgaca ttaccctgaa ggcgctcaac ggtgtaatag ctggaatgct gaaagacgga
     4801 aagagcctac atgagtgttc accgcaagag aaacgcctgg tagctcaact gaaagcatta
     4861 cgcgccgatc ttcaccatca gctggcttca gtcggttgcg acctggagga ttaaaatatg
     4921 aaacaagaat ttttaaccgt tgcgacacaa tcagcagacg ttcgcccgga agctgagctg
     4981 aaccgcgcag aacgcatcaa agcagcctta atagccatca aggcggaatt attcaacatg
     5041 tcggaagaag acttgctaca gcttcaacag gacgtaaacg ccgccgtaca gaagcgcaat
     5101 caggtcagtg aagagaacaa agtggctgag gctggattaa aagtccatca gcatcatcag
     5161 gccaatgtgc catcgtggaa cgactacttc taagaagggg aaaaattatg gaactgaaag
     5221 accgcattat tcagaccaaa tgcgaaagcg atccggtatt ccgtgagcgt tacctacgcg
     5281 cccaggtcaa accgctggct gaagttcagg acttaatgcg tcgtggttgt agtgacccat
     5341 tggcacagga gaaagcacga caggccatca ttgatgccgt tgacctgcta cttgcccatc
     5401 cagaggcagc gcatcacatc atgaacgtgg tgaaagcagc acatcagctt acctggcaac
     5461 aagcaccgaa atcatcagag ccgctaaacg attctgacct ggaacagcag attgaagacg
     5521 agttttaatg ccatttttct tcgaattctt cgctaaaacg gggccaaaac ggccctataa
     5581 acaacaaaac aggagcaaaa attgagataa aagaggtgat ttatgactga taatctgccg
     5641 caaacgccag ccagccggac ttttcagaca agtaaagggc aaaatggacg cccaacaaaa
     5701 tacaatgacc gcatagcaaa acagatttgc ttatatgttg cggaaggaat gagcctcaac
     5761 gaaatctcga aaatccccgg aatgccttgc cgggcaacgt taagcgtctg gcagcttgaa
     5821 cacccggaac tgaggcagac tatcgaggga ttaaggttta ttgtcgccac cgattacgct
     5881 tgccaggcgc tggaatgtta cgacgacctc gatccagcca gcgagaatat ccgggctgaa
     5941 ctagccattg ccaaagcgaa agcggcaacg ttactcggca tggcacgact acaggatttg
     6001 cgtaatctgc cgcgaccaat acaggagaat gaagaatgtt aatccgcgac caggacaaag
     6061 gtaaacgcag ccgccttgaa gacggttatc gctcgctgga gaacaagaaa ttccaggact
     6121 acaccgatga agacctgcgg aaaatcggca ccattgacgt ggcactggaa ctgtacacca
     6181 ccaactacga acaagggagc caaatggcgc tgttagaaat ggctgacaag atcaacaacc
     6241 acgaagtcgc catcatcgac gactgcctct gtatgcgccg catcgcgccg ccgctgcctg
     6301 ggatgattca caataaggcg gtactgactg acccggcgtt cgcctatatg tggacgggat
     6361 acgtgaaagt cattgctgtt catagcctga actacaaaga cacattcaaa gcaaccgacc
     6421 gcgcccggta ctggcgcatg gtccgcgaca atgagaagcg attgaatgaa gatctgccag
     6481 ttggtggcta tgacgccgtt gacctccagg gtatcgcact ggctaacctt cgtgcccgac
     6541 cggactacag cgacaaccct cacgccgatt gcgaaggcat gtttgtaccg gaagattacg
     6601 tgccgcaagc gccggagcaa atttagtttt cggacaagta cgcgcgcgca gccgacgcca
     6661 gggggataga ctggcggcct tcaaacgccc cgattcaggg catttagcct aagtcattga
     6721 tttttcaatt atccaaatct ggataatgcc gaccatcacg ccacaaatgc cctacgagaa
     6781 aaatccctca caactaccct gccaattttt cagatttcac caaaacgcgc ctgattcgat
     6841 agcgtccgca accatgtttg attgaacacc gggtttttac ttgatgcgca tcaaaaaagt
     6901 gttacaaatg ccttttatcg aggggtattt gctcttaatg tgtccgtaat gaagaagcaa
     6961 gggtatttga acacaggggg cagtcatggc aaacatcgct tacattcgcg tgtcaactgt
     7021 tcaacagaac actgagcgcc agtatcacgc aatgaaagat attgcgatcg acaagtggtt
     7081 tgaagagaag aagtctggca agaacgaaga ccgcgaacgg ctgaaagaaa tgctcgagta
     7141 tgccagagaa ggtgaccacg tatacgttgc cagcctggac cgtttggggc gcaaccttat
     7201 cgggattctg aaaatggttg aagacctgag agaacgcggc gttgttctcc attgcctgaa
     7261 agaaggttac gtgctgggcg ctgagaaaaa cgcgttacag gaggcgttct tccagatgat
     7321 gggagtgtta gcccaactgg agagaaacat catgttagag cgtcaggcgg aagcgtacgc
     7381 agcagcaaaa gcggcgggta agcagattgg tcggggtaag agtaaacgtc gccaggagag
     7441 cgaagagttt gttatcgagg cgctcaaaaa cggtcaccag ccagcgaaga ttgcccgtgc
     7501 gcacaagatc agccggacaa gcgtttaccg tatccgtgac caggctatca aagagggcaa
     7561 gctaccggaa aacttccgcg ttaaagccat caactaaccg caaaacaaag gggagcaagc
     7621 gttagcctgt tcccctttct tgtaccctct cacaatcccc cgattgccat cacattttaa
     7681 cgccacacca tttcatcgtt cacgtttagc cgtagaaagg cattttgtga cacccttcac
     7741 aaaaactctt tcatcgagcg gttactgccg atacaagact aatcaaattg attaaggtca
     7801 atgtttatca ccgtgcgcag cgttatttat aactcaacgg aagggaaacc cttcaaacga
     7861 cggaaaccag gcgatgaaag ggaggtgtga aatgggtatt cgcaacaatg ccggatggtg
     7921 gaaacggttt aaacacttcc accctgagct ggggttagct gccgaaaccc tgggcgtgct
     7981 gatgatgatg ctggtgttat gtgtggagtt ttagctatgg atataaaacc aatcgatcca
     8041 cgctggaaac cgagggatat ccttcgcgtt ctggcactaa tcaaacaact ggaagcgaaa
     8101 aaaaaggagg ttgactggag aaaggataaa agagattacg acaacgagga agtgagaaaa
     8161 aaatggaaca gcatttacaa aacattccaa actaagaaaa gccaaaaaac agaaagttac
     8221 caagtccgct taaagaatgt cttatggtgg gaaaaccacg cacaagaagc gcatgagtca
     8281 tattgccgcg cgttggatga gttcaaatct ctgaaagggg agttagaaac aatcctcgcc
     8341 aggtacggat tgaaacctaa ccttttgaat ccagattgat agctttaaga acaacgaaaa
     8401 cacccggccc cgcgccgggc tttttgtgat ctgcgtcatc ttttagcgat aatcaccgac
     8461 aaattatctt cgctagtgat agcctttaat cgagattaaa tcatcgttga aagggttgga
     8521 ggttgaaatg agcatcaagg ggccaatgga tcttatcctg agagaaacag ccagaaaaag
     8581 cggatggtac aacgacaaca atcagccgcg tattcgtgac attgacgcaa ggctaaacat
     8641 gccgcgcgcc ggattccagg ccataggcac gtatctaaaa ggtgagaaag agccacgctt
     8701 agaaaacctg gcgagaatgc tggatttgtt cgatgtggcg gcaattatgc cattgaaaga
     8761 tggaagctgg cttgtatgtg aaccatgcgg cggcgcggat ctggtaagcg agaataagga
     8821 attacgcgca cgtgtcgcgg aactggaaca gcagatcgca gaaatggaag taaaacacga
     8881 aacccttcca acgacggaaa acactgatga aagggtcgat gaaagggtcg atgaaagggt
     8941 cgatgaaagg gtcgatgaaa agcacgtaag cagaaagcgc aatcctaaac cgcagctatt
     9001 tacggtcgct gaccttaagc acgctttaat cgagaacggc ggcacgttga aagggcaaaa
     9061 cgctgatata gctttcgcga tggcacttaa ctacagcgac atagcaacgc gccgggtaga
     9121 aacattacaa ccatctatcc ctctggtgag ccgtctggtg aaagcagcaa gtgaggccgg
     9181 aattatccgc gtgacgcgcg aagggagcca gggaccgaag acgttaacag tcttgtaacg
     9241 atgataaatc atgcgatgaa agggaatcag catcatttca tgaaacgcaa cgacatgctt
     9301 gattgttgcg aaaagtgaaa taagccgctc aaatcaacag tttgcgagag ttttttaata
     9361 gcagtatcgt agaaagcaga aagcccggcg gcatccgggc tatcaatgaa accaacattc
     9421 aacaggagat tcaaaccatg atgaaaaatg ttgtcagtca attaacgtgg gagatagatc
     9481 gatgaaaaaa tcaaatcaag cgttaaccga cgctttaagt ctagcaaccg ccgccgcgca
     9541 catcaactac agcaaaggcg aaaaggttgg agaatggata ctatgcgcga acgtgcctga
     9601 acaaaaagtt atagacgccc tgaagaaagt taagttcgct gtggtcgatg gcg
//
