LOCUS       CP057329                2001 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Citrobacter freundii strain RHB30-C03 plasmid pRHB30-C03_7, complete
            sequence.
ACCESSION   CP057329
VERSION     CP057329.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148288
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH02 T3-C03
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183.227035180809x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 11:16:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,259
            CDSs (total)                      :: 5,142
            Genes (coding)                    :: 5,081
            CDSs (with protein)               :: 5,081
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 17 of 61
            Pseudo Genes (incomplete)         :: 49 of 61
            Pseudo Genes (internal stop)      :: 13 of 61
            Pseudo Genes (multiple problems)  :: 12 of 61
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHB30-C03"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:546"
                     /plasmid="pRHB30-C03_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            1516..1752
                     /locus_tag="HVY71_26295"
     CDS             1516..1752
                     /locus_tag="HVY71_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMF95586.1"
                     /translation="MNRLTNLTPAEKKFLDDAIAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREGERQQSEFAWSRPRTPRR"
                     /name="hypothetical protein"
     Typing          1180..1352
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.205"
                     /coverage="100.0"
                     /evalue="4.4699999999999994e-54"
     Typing          complement(650..1201)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.394"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 taggtcgctg taatccacta tcgccaaccg ataagcattg gaatatgacg cttatatcat
       61 cccctgggag cgagctaaac gtagagtgat ccatgttctt cacaaggatg gtcttgcctt
      121 gattaaggca atattggttc gcttctgtta acgcagcttt ttttgctcca gcctgtcccc
      181 ttacaggtgc agcatgaaca gatatggaat aagtatctgg ccccatgggt aatacgccac
      241 ttgaattaac gcatcctgta agggaaatca ttactgctgc aaagcaaatg tatttcatta
      301 ctctaatatc ctatattttt atttttctat ttattcaaat ggttatgctg gtttgttggg
      361 tgattgtgga tcatccaact atacgatttt ttatgcttta ttttcaaagg gttttgtcgg
      421 tgtgagtttt tgactggtac tgttgtttgg tatcggtttt actttgatgt ctgtcggtca
      481 tcgaagatga acctcttgag atcctttccg ttgcgtcgta atctcttgct ctgaaaacga
      541 aaaaaccacc tggggaggtg gtttttcgaa ggttcagtaa gttggggaac ttctgaaccg
      601 tggtaacagg gtgtacaaga ccgctgccac caaattcgtc cttccagttt agccgtagtt
      661 tggctctgac ttcaagaact ctgcatcagt aatctcttgt acaccctctt accagtggct
      721 gctgccagtg gcgctttgtc gtgtctttcc ggcttggact caagtcgata gttaccggaa
      781 caggcgcagc agtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact
      841 gccttcccgg aaccgagtgt caggcgtgga atgagataaa cgcggccata acagcggagt
      901 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccac cagggggaaa
      961 cgcctggtat ctttaagtcc tgtcgggttt cgccaccact gatttgagcg tcagattctg
     1021 tgatgcttgt caggggggcg gagcctatgg aaaaacggct ccgccgtggc cttctcgctt
     1081 ccctgttaag tttcttcctg gcatcttcca ggaaatttcc gccccgttcg taagccattt
     1141 ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata
     1201 tcctgtatca catattctgc tgacgcgctg gtgccacctt tttctcctgc tacatgaagc
     1261 actttcataa agtgttactc cgccgtaaca tattaagcca gtatacactc cgctagcgct
     1321 gcgtgactgg ttcagggctg cgccccgaaa cccgctaaca ccactgacgc gcctgcggct
     1381 tgcccaacgt ctcacgtcac cggattaaaa tccgctcccg ttcaccgttt ctggctgtct
     1441 ttcagcaccg ccagcgcttc atcggttctg ctgtgtctgg cagactgtgc ggagttattc
     1501 tggcgggaga atgctatgaa cagactgaca aacctgacac ctgcagagaa aaaatttctt
     1561 gatgatgcta ttgccgcagc tgagagagcg tcaggcaaaa agctaaatca gccgaaccgg
     1621 catatcgtcc tgaacagagc gcgtgcgcag attgagtcgc agcgttacgc cgaccgccag
     1681 cgtgcattac gtgagggtga gcggcagcag tccgagtttg cctggtcacg tccgcgcacg
     1741 ccgcgccgct gattgcgcct tttggcggtt tagtggtgct gacactccga aatcgtctgc
     1801 cattcacccg cgtgcatagc catgcagtcg cgcagaaatc atcaggtgcg tttttcaggg
     1861 ttatggccag ggaaaggtgc tggaaatggc taactcgtta cagagtggcg ctgagggcct
     1921 ttcagagggt gcgtccaaca gatgttatgg taaatggtgc ctaattaacg ttgacgttta
     1981 catcagcagg actttgataa t
//
