LOCUS       CP057328                3370 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Citrobacter freundii strain RHB30-C03 plasmid pRHB30-C03_6, complete
            sequence.
ACCESSION   CP057328
VERSION     CP057328.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148288
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH02 T3-C03
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183.227035180809x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 11:16:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,259
            CDSs (total)                      :: 5,142
            Genes (coding)                    :: 5,081
            CDSs (with protein)               :: 5,081
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 17 of 61
            Pseudo Genes (incomplete)         :: 49 of 61
            Pseudo Genes (internal stop)      :: 13 of 61
            Pseudo Genes (multiple problems)  :: 12 of 61
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="RHB30-C03"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:546"
                     /plasmid="pRHB30-C03_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            800..1045
                     /locus_tag="HVY71_26285"
     CDS             800..1045
                     /locus_tag="HVY71_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMF95584.1"
                     /translation="MGFWDKARDVAKSAGKMAWEETKAANERMQQYKDEMPSLNDRELA
                     RIVAKERSSSPLKAGAARQELKNRGYSTAEEIKSLL"
                     /name="hypothetical protein"
     gene            complement(2683..3363)
                     /locus_tag="HVY71_26290"
     CDS             complement(2683..3363)
                     /locus_tag="HVY71_26290"
                     /inference="COORDINATES: protein motif:HMM:NF025107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QMF95585.1"
                     /translation="MIPRWAPMYSWWIQDEVIQRLNWRNNELGRPAAKTAALMLYIAIT
                     MKSSEEPGMSPVKITYNRLSEMTGISRELIREGITILESLEMISVEKVGRNNIYKLINK
                     RMKGGWCKIPVKPLMTEDGKVISPFKELRLRRKIELHAIKIFLYLASVRDNHTEFSVAS
                     YETIRKAIGASDKDISRALTLLSIIGLLARVSSEKAEDNKVNQPNRYYLIGYKSFARSS
                     EINL"
                     /name="helix-turn-helix domain-containing protein"
     Typing          2140..2596
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.0"
                     /coverage="90.0"
                     /evalue="6.75e-87"
ORIGIN
        1 cctccataat gcaataccga ttacgacaaa agccgtcaaa acggcaagag ataaaacgaa
       61 tggtacaaag cccccaataa caggagccaa agtggtcatc aatgcactta cattttctaa
      121 aatttccata aaagctcctt ttttggctgc gttatttttt aattttaccg ggctcaatta
      181 tctctatcta gaagtagtgg cttttgtata gataaaaaaa atcacaaaaa aacgttatta
      241 atcaaattca taactcttaa aaattcagtt ttttaaatat gaaaattagt tcataaagac
      301 gtgttctaag gatttaatag ctcaaaagag ctcaataaac gcacaaaaaa ataatgtaat
      361 tagttcatga aaacaccaca taagaccatg aaaaataaga tttattttta agttattgtt
      421 ttatatgtaa attaccaatc ggccaattac accaaatgtc ttaaattcgc ttcacgagtt
      481 gcctcaaaaa atgcaggtaa attcgccctc attgatggct ttttgcatca gctaatccct
      541 tgtattgaac ataacatccg gctgacgaac cgagatctct gacttttaag ctcaagtaac
      601 ctccagcttt gccgatgtag agtgcactac actggcagat aaaagaggtt ctgaatggca
      661 ccaacaatat acagggctaa gtgcttggaa tgtaagcatg aatggactag tcgagcaggt
      721 tatggaaccc cagactactg cccaaaatgc cgcagccgta gaatttcaac aagcccaatt
      781 ggataagcag aggtaacaga tgggattctg ggacaaggcc cgcgatgtgg caaaatccgc
      841 aggaaaaatg gcttgggaag aaaccaaagc tgcaaacgaa cgaatgcaac agtacaaaga
      901 tgaaatgcca tcacttaacg atcgtgaact ggcgaggatt gttgcgaagg aacgcagtag
      961 ctcaccacta aaagccggtg cagcaagaca ggaattgaaa aacagagggt actcgactgc
     1021 tgaagagata aaatctctcc tgtgataaca ggccgctctt agcggctttt ttgtacccgt
     1081 cggtcacctg catttaccat aacatctgtt gcccgcaccc tctcagacag cctcagcgcg
     1141 tttctgggtc ttcctgcctg tttccggcac ttccctgtgc ctatatcgtg aaaaaacgcg
     1201 cctgataatt tctgcgcacc tgcatgacta tgcacgcagg tgactggcgg gcggtttcag
     1261 gcgtatgttg tcactaaggg cggattttag tacgtaaagg cgactgacgg tattggttaa
     1321 tctgtacctc attctcacca ctgctgcttc agccactacc gcacaaatca ggggttcccg
     1381 gccctgcgtg acgggcgata tagcggttgg taaatctgta cctgattaac ggtgaacggg
     1441 tgcgcagttt actgtgcggc cgtgaggcgt tggccaggcc gtcaggcatg gctggattta
     1501 ttgctaaggg gggggtcgga tttaacctgg ggtgatcagc gcgcagagat gaacgggatt
     1561 cggaagggga acccttgcgg cgcggcgatg tatacatcgc taacgtgcct tactcttttc
     1621 actgctgagg gtagcgacag caaacagata gcaaaaaggt agcaaacaga tagcaaacag
     1681 atagcaaaca tacggtgtcg ggcggagcct gaccaggagt cattggcgtg ccggcggtac
     1741 tccaaaaaca catcttgtcc cgctgttttg cgaataagcc agcatggctg tgggcacaaa
     1801 acatttcctg tccgatgttt taacgtttgt aatccaagag cgtcaggtga cgtcactttg
     1861 cggcgttagc cctatcctgc atcgtattaa aaaatgcagc tatggcgtgt acggtgccag
     1921 agataagtac ggagatatgc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1981 ctgcggcgag cggagggaac agagacatta agacataggc aatgagagag gatggagaag
     2041 caacaggaga ggccgaggcg ttgccggttt tccataggct tcgccctcct gacgagcgtc
     2101 acagaaatca gacgatctcc ggtgctcaaa tcagaagggg cgaaacccga caggacttaa
     2161 agatccccac cgtttccgga aggtagctcc ctcgtgcgct ctcctgttcc caccgtacag
     2221 tttacccatg tctatccgct gttatggccg cgtattctca ttccacgcct gacactggat
     2281 tctgcttagg tggttcgctc caagcttggc tgtatgcacg aactccccgt tcagcccgac
     2341 taccacgtct tgttcaggta actatgctct tgattccaac cctgacagac acgacgaatc
     2401 gccattggcg gtagccaacg gtaacagaga tgtgcgagag atattctgag aactcttgaa
     2461 gtagaagcct gactacggct acactggaag gacagatttg gtgacatgtc tcgcacatag
     2521 actgttacca cggttcagaa acttgccggt tactgaacct tcgaaaaagc caccttgccg
     2581 ggtggttttt tcgtttcaat cgtaagatac gctgacggcc tcaacatcgt caagacagca
     2641 ttccgagcat tactttgatt taaaggccat atttgtagct tcttaaaggt ttatctcact
     2701 acttctggcg aagctcttat aaccgatcaa gtagtatcga ttgggctgat ttaccttgtt
     2761 atcttctgct ttctcgctag acactcttgc aagcaatcca ataatcgaaa gcaacgtcaa
     2821 agcacgagat atatccttat ctgatgcgcc aatagcctta cgaatggttt cataagaagc
     2881 gacagaaaat tcggtgtgat tgtcacgaac tgaagcaaga tagagaaaga tcttaattgc
     2941 atgaagctct atcttacgtc tcagtctcaa ttctttaaat gggctaatta ccttaccatc
     3001 ttccgtcatt agcggtttta cgggaatttt gcaccatccg cctttcatcc gtttatttat
     3061 gagtttgtag atgttgtttc ttccaacttt ttctacagaa atcatctcca aactttcgag
     3121 tattgtgatc ccctcacgta tcagctccct gctaatccct gtcatctcag acaagcggtt
     3181 atacgtgatt tttacaggac tcattcctgg ctcttcagag gatttcatcg taatggcgat
     3241 atagagcata agagccgcag tcttcgctgc cggccttcca agctcattgt tccgccaatt
     3301 taaccgttga atgacttcat cctgaatcca ccaagaatac attggagccc aacgaggtat
     3361 cattttactt
//
