LOCUS       CP057307                7592 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB30-C09 plasmid pRHB30-C09_6, complete
            sequence.
ACCESSION   CP057307
VERSION     CP057307.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148293
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7592)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH02 T3-C09
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 132.647008810688x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 07:59:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,680
            CDSs (total)                      :: 5,553
            Genes (coding)                    :: 5,179
            CDSs (with protein)               :: 5,179
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 374
            CDSs (without protein)            :: 374
            Pseudo Genes (ambiguous residues) :: 0 of 374
            Pseudo Genes (frameshifted)       :: 134 of 374
            Pseudo Genes (incomplete)         :: 213 of 374
            Pseudo Genes (internal stop)      :: 59 of 374
            Pseudo Genes (multiple problems)  :: 31 of 374
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB30-C09"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB30-C09_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(302..1879)
                     /locus_tag="HVY76_28270"
     CDS             complement(302..1879)
                     /locus_tag="HVY76_28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="QMF75885.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFTE
                     EELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPIAK
                     NGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSIISSA
                     MAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSLYTGR
                     TDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQAETDQ
                     IATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGEIRQATQ
                     VIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAA
                     RTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGIGQRVNEIT
                     DMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQLTSALSELA
                     KQFWEKRG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            2189..2749
                     /locus_tag="HVY76_28275"
     CDS             2189..2749
                     /locus_tag="HVY76_28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QMF75886.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            2753..5719
                     /locus_tag="HVY76_28280"
     CDS             2753..5719
                     /locus_tag="HVY76_28280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="QMF75887.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRGD
                     ANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRREHA
                     QELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALSVID
                     RACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKPNSRH
                     MLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYATLAAV
                     VLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIGQALLEA
                     KESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRYTPALLEV
                     LELRAAPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKFYE
                     ICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQYLE
                     ERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLPRI
                     KITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPGLTY
                     AKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRGESTG
                     HVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIEEHYTD
                     TAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTLNIKHVRAH
                     WDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVEL
                     RRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYL
                     ERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFRPLRMPGKP"
                     /name="Tn3-like element TnAs1 family transposase"
     gene            complement(6634..6825)
                     /locus_tag="HVY76_28285"
     CDS             complement(6634..6825)
                     /locus_tag="HVY76_28285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMF75888.1"
                     /translation="MLKKDDIILIKSYGNEKTKIFLRGVNEVLMIDASFEEVKFAINND
                     LQDLQGMQAVSAPLKIAT"
                     /name="hypothetical protein"
     Typing          2..106
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.283"
                     /coverage="98.0"
                     /evalue="1.64e-39"
     Typing          7483..7587
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.916"
                     /coverage="98.0"
                     /evalue="1.68e-24"
ORIGIN
        1 catattaagc cagtatacac tccgctagcg ctgcgtgact ggttcagggc tgcgccccga
       61 aacccgctaa caacccctta gctactggat atcaccccat gccatctgag tagggggaac
      121 cgcagaattc ggaaaaaatc gtacgctaag ctaacggtgt tctcgtgaca gctctttgac
      181 taggctttct aaggccattc tgatagccct gacttcctga aaagccatgg ctaaaatttg
      241 tgcggctaaa agggataacc gatggtaaag taagttatcc ctgtcgagat actgaaaagc
      301 gttatcctcg tttttcccaa aactgttttg ccagttcgct cagagcgcta gttaactgag
      361 cgacagattt cgcactttgc aaattattct gcccggtctg tacattggta tcagcagcat
      421 cgcgtatatt gataatactg cggtttatgt cttcacttac tgctccctgc tgctcgaccg
      481 cagtcgctat ttgcgcgttc atgtcggtaa tttcgttaac gcgttggcca attccatcaa
      541 gagctgtagc tgcttcctct gcgtgagcta cactcgtgtg cgcttgccga ctactttgct
      601 ccatgactgt aacagcggat tgcgctcgct cttgtagagc gctgatcatg ctttgaatat
      661 ccgttgtcga ttgctgtgtg cgagcagcaa gactgcgaac ctcatcggcg acaacagcaa
      721 aaccacgccc ctgctcacca gcacgcgcgg cctcaattgc tgcgttgagt gccaacaaat
      781 tcgtttgctc ggcgatccct cgtataacgt caagaacttt tgatatctcg ttactttgac
      841 cttcaagctc atgaataacc tgagtggctt gcctaatttc accttcaagg gcagtgattg
      901 actggcttgt gtgggctacc agacgctggc cagatgccgt ctcagtgtct gctcttccgg
      961 ccgcatctgc agcatgctgt gcattgctcg caacctcttg aatgcttgcc accatttggt
     1021 ttactgccgt tgctatttga tctgtctctg cctgctgctc aactgtaagt acattgcttg
     1081 actcaatatc ctttagtagg cctcgggtgt gttcgctaag ccgatttgat gcatcaccta
     1141 tgcgacctac tatggcgcct gtttcagctt gcatcattcg taaagcaaac tctatttggc
     1201 caaactcatc ggtgcgccca gtgtagaggg attgacttaa tgggttattg gaaatattcc
     1261 tggctctttc aaccagtctt ccaagaggag agagaatagc caaaacacta acagagctta
     1321 agcttcctga cattaaagtg gctaacaata agctgcttat tgatgtatca gtaagcatgc
     1381 cggcagccat tgcgcttgat ataatactac cccatatgag caagagtatt ttcacggaaa
     1441 agctagcagc caatttcggc ctcgcggcct tcccgcttct caattgagca tataattttt
     1501 ccgcagccaa aacctgctca ggttcaggct tggtccttac agactggtat tcaacaatcg
     1561 aaccattctt agctattggc gttacataag cacttaccca atagtggtcg ccatttttac
     1621 agcgattttt tactagcccc atccatgagc ggccagattt taatgtactc cacatatgct
     1681 caaatgcagc aggcggcata tctgggtgtc ttacgatgtt gtgaggctgg cctaatagtt
     1741 cttcctcagt gaaaccactg attttaatga agtcaggatt aacgtacgtg atatggcttt
     1801 gaggggaggt agtcgaaaga atattggcat cttttgggag ttctaagttt cgacccgtca
     1861 ctggtaaatt ctggcgcata agaacctcaa gggttggctg ttttatttta ttgttttcgg
     1921 cattaagccc aatttcttga gcgttacgat aaagctagca tggaaacgat aggtgcaagc
     1981 aagttaaggg ttgcatcgcg catgtcaatc taggctatac cctaacttga tgtcaggcag
     2041 ggccgcgccg cttcgtcaga atagagtctg ctttcccatt ttttgacaca tgcccgcgaa
     2101 ggttatagat ttcagcctga cagaaatggg ctttgaggca caacggaaca gaaagtgcac
     2161 ttaagccgcc ttcaaccaag gagacatcgt gcaggggcac cgcatcggct acgtccgggt
     2221 cagcagcttt gaccagaacc cggaacgcca gctggaacaa acccaggtga gcaaggtgtt
     2281 caccgacaag gcatcgggca aggacaccca gcgcccccag ctcgaagcgc tgctgagctt
     2341 cgtccgcgaa ggcgatacag tggtggtgca cagcatggat cggctggccc gcaacctcga
     2401 tgacctgcgt cgcttggtac agaagctgac tcagcgcggc gtgcgcatcg agttcctgaa
     2461 ggagggcctg gtgttcactg gcgaggactc gccgatggcc aacctgatgc tgtcggtgat
     2521 gggggccttc gctgagttcg agcgcgccct gatccgcgag cggcagcgtg agggcatcgc
     2581 cttggccaag cagcgtggcg cgtaccgggg ccgcaagaaa gccctgtccg atgagcaggc
     2641 tgctaccctg cggcagcgag cgacggccgg cgagcccaag gcgcagcttg cccgcgagtt
     2701 caacatcagc cgggaaaccc tctaccagta cctccgcacg gacgactgac acatgccgcg
     2761 tcgcttgatc ctctcggcca cggagcggga caccctgctt gcgctgccgg aaagccagga
     2821 tgacctgatc cgctactaca ccttcaacga ctccgacctg tcgctgatcc gccagcgacg
     2881 cggcgacgcc aaccgcctcg gcttcgccgt gcagctctgc ctgctgcgct accccggtta
     2941 cgcgctggga accgacagcg agctgcccga gccggtcatc ctgtgggtgg cgaagcaagt
     3001 ccaggccgag ccggcgagct gggcaaagta cggcgagcgc gacgtgaccc gtcgcgagca
     3061 tgcccaggaa ctgcgcacct acctgcaact ggccccgttc ggcctgtccg acttccgcgc
     3121 cctggtgcgc gagctaaccg agctggccca gcagaccgac aaaggcttgc tgctggccgg
     3181 tcaggccctg gagagcctac ggcagaaacg acgcatcctg ccggcgctga gcgtgattga
     3241 ccgggcctgc tcggaagcca ttgcgcgagc caatcggcgg gtctaccgcg ccctggtcga
     3301 accactcacg gactcgcatc gggccaagct ggacgagctg ttgaagctca aggccggcag
     3361 cagcatcacc tggttgacct ggctgcgcca ggcaccgctg aaacccaact ctcggcacat
     3421 gctggaacac atcgagcggc tgaagacatt tcagttggtg gacttgcccg aaggcctggg
     3481 ccggcacatc caccagaacc gcctgctcaa gctggcccgc gagggtgggc agatgacgcc
     3541 caaagacctc ggtaagttcg agccgcagcg ccgctacgcg accctggccg ccgtggtgct
     3601 ggagagcacc gcgaccgtga tcgatgagct ggtcgatctg catgaccgca tcctggtcaa
     3661 gctgttcagc ggcgcgaagc acaagcatca gcagcagttc cagaagcagg gcaaggcgat
     3721 caacgacaag gtgcgcctgt actccaggat cggccaggcg ctgctggaag cgaaggaaag
     3781 cggcagcgac ccctatgccg ccatcgaggc ggtgattccc tgggacgagt tcaccgagag
     3841 cgtcagcgag gccgagctgc tggcccggcc ggaaggcttc gaccacctgc acctggtcgg
     3901 cgagaacttc gccaccctgc gccgttacac gccggccttg ctggaggtgc tggaactgcg
     3961 cgccgcgccg gccgcgcaag gcgtgctggc agccgtgcag accctgcgtg agatgaacgc
     4021 cgacaacctg cgcaaggtgc cggccgatgc acccacggcc ttcatcaagc cgcgctggaa
     4081 gccgctggtg atcaccccgg aaggcctcga ccggaaattc tacgaaatct gcgccctgtc
     4141 cgagctgaag aacgccctgc gctccggcga catctgggtc aagggctcgc ggcagttccg
     4201 cgacttcgac gactacctgc tgccggccga gaagttcgcc gcactcaagc gcgagcaggc
     4261 cctgcccctg gcgatcaacc cgaacagcga ccagtacctg gaagagcgtt tgcagctgct
     4321 ggacgagcag ttggccaccg tcacccgcct ggccaaggac aacgagctgc ccgatgccat
     4381 cctcaccgag tcagggctga aaatcacccc gctggatgcg gcggtgccgg atcgggcgca
     4441 ggcgctgatc gaccaaacca gccagttact gccgcgcatc aagatcaccg aactgctgat
     4501 ggacgtggac gactggacgg gcttcagccg ccacttcacc cacttgaagg acggggccga
     4561 ggccaaagac aggacgttgc tgctgtccgc aatcctcggt gatgcgatca acctcgggct
     4621 gaccaagatg gccgagtcga gccccggcct gacctacgcc aagctgtcct ggctgcaagc
     4681 ctggcacatc cgcgacgaaa cctattcggc ggccttggcc gagctggtca accaccagta
     4741 tcgccacgcc tttgccgccc actggggcga cggcacgacc tcatcctccg atggccagcg
     4801 cttccgcgcg ggtggccggg gcgagagcac cgggcacgtc aacccgaagt acggtagcga
     4861 gccgggacgg ctgttctata cccatatctc cgaccagtac gcgccgttca gcacccgcgt
     4921 ggtgaatgtc ggcgtccgcg attccaccta tgtgctcgac ggcctgctgt accacgagtc
     4981 cgacctgcgg atcgaggagc actacaccga cacggccggc ttcaccgatc acgtctttgc
     5041 cctgatgcac ctgctaggct tccgcttcgc gccgcgcatc cgcgacctcg gcgaaaccaa
     5101 gctgtacgtg ccgcagggcg tgcaagccta cccgacgttg cgcccgctga tcggcggcac
     5161 cctgaacatc aagcacgtgc gtgcccactg ggacgacatc ctgcgcctgg ccagctcgat
     5221 caagcagggc accgtcaccg cctcgctgat gctgcgcaag ctcggcagct acccgcgcca
     5281 gaacggactg gccgtggccc tgcgcgagct gggccggatc gagcgcacgc tgttcatcct
     5341 ggactggctg caaagtgttg aactgcgccg ccgcgtgcat gccggcctga acaaaggtga
     5401 ggcgcgcaac tcgctggcca gggcggtgtt cttcaaccgc cttggggaaa tcagggatcg
     5461 gagcttcgag cagcagcgct accgggccag cggcctcaac ctggtgacgg cggctatcgt
     5521 gctgtggaac acggtgtacc tggaacgcgc cacccagggg ttggtcgagg ccggcaagcc
     5581 ggtggacggc gagctgctgc aattcctgtc gccgctgggc tgggagcaca tcaacctaac
     5641 cggcgattac gtctggcggc agagccgcag actggaagac gggaagtttc ggcccttacg
     5701 gatgcccgga aaaccttagc gtacgatttt ttccgaattc tgcgggctcc ccagtatgat
     5761 gaacaacggg atgcgaatga tgcgcagctt cccggcgaat atgacctttt tgatttaatg
     5821 gcgcaggtta ctgaagagaa cctgcacgcc ccggtggatt ttgggccgcc tgtcggcagg
     5881 gaagcgttat aacttttttc gctggtcagc gtacgtgtaa tacgccggta ttacatctta
     5941 tttctttatt ttcatgtgat tacaggtggt tgtaatacgt tgtgtattac ataaataaac
     6001 gggaaattca gtttttacct tttaattcat gcaattgcga tgaaaatcgc ggttgcgtat
     6061 ggtgtatgac cattttaagg ttgaaaaata atcaactatg gagggtgatt tacgcaggtt
     6121 aaggggagat cgtgacaaaa aaagcgttcc tcactgtatc gttcatgcct gcatgaacgg
     6181 gttattcttc ctgactacag aattacatca aaagtacatc aaaattacat cacttttacg
     6241 tcacttttac atcattttta cgtcagatct gacctctgaa ctattcgtac atccgcccct
     6301 cttcctgggc ctcctgcacg tgctgaaaat ggtttttccg gctgtgccgg caacgcctca
     6361 cggccacgca ataaattgcg catccgttca ccgttatcgg ggtggttcgt ggtggctttc
     6421 agggcgttca tcggatttta gccgctaaaa tcccggccag gtatgtctgt gcatagccat
     6481 gcagtcgcgc cagaatcatc aggggcgttt tttctcgata tgggcacaga aaggtgtgtg
     6541 tttcaggccg gaggcctcag aaacgccctg agggcctctc aggcggtgca cgcaacggat
     6601 gttatggtaa atgttgagtg ctcttactaa aaatcaggtt gctattttta gcggtgcgct
     6661 tactgcctgc attccctgaa gatcctgaag gtcattattg atagcaaact ttacctcttc
     6721 aaagctcgcg tcaatcatta gaacctcatt cacaccccgt aaaaatattt ttgttttttc
     6781 gttaccatat gattttatta ggataatatc atcttttttt agcatgatac tttcgttact
     6841 tattgttttg agatgaagca tcgtcatggt tttttttgca tgtgcttgtt aaggggattg
     6901 agattattcc taactccttg agtttgtaca tacaaaaaaa acaaaaaaat cctaatgccc
     6961 ccgtggtggg tatataaaaa tcacggggag cgcagcgacc ttcgtccagc tcgtttcttg
     7021 tgtgtctcac cctgcaaaag gtttgattgt gtgggcgctg tgctgacgtc tgagaatcac
     7081 actttgccgc ttactgctgt cttattcgct cattttatca ggtgcttgtt atcgtacagc
     7141 gagacggggt agagcctaac tctttcgcgg ttaagttacg ttcccaccaa tttgcctgtt
     7201 tatcctgccg gagtccgggg cgtagacctg agcgtctaag gtgcagatat gattttaatg
     7261 tcgttgaatg gtattaagct ggtagtgaca tcgctagcca gccctgctag gatttagatt
     7321 ctcacgtctg atcctggcag gtatctgcga agaattagag taccgaagcc ctgtgattct
     7381 gtcacggggc ttttgtacat ttagcgcccg agaatcaaaa acatcccttc agaacttatg
     7441 taacgcccgc cgcagtctca cggcaggagc gcagcgaccg agtgagcgag gaggcgacat
     7501 ttcatcctta cctgcatgtt gcacttacgc cccgcagcta catttttccc ctgccacatg
     7561 aagcacttcc ctgaattccc acttcgcttt ac
//
