LOCUS       CP057298                7592 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB30-C10 plasmid pRHB30-C10_6, complete
            sequence.
ACCESSION   CP057298
VERSION     CP057298.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148294
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7592)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH02 T3-C10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 132.442474658053x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 07:43:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,692
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,192
            CDSs (with protein)               :: 5,192
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 373
            CDSs (without protein)            :: 373
            Pseudo Genes (ambiguous residues) :: 0 of 373
            Pseudo Genes (frameshifted)       :: 134 of 373
            Pseudo Genes (incomplete)         :: 212 of 373
            Pseudo Genes (internal stop)      :: 59 of 373
            Pseudo Genes (multiple problems)  :: 31 of 373
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB30-C10"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB30-C10_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(7557..7592,1..525)
                     /locus_tag="HVY77_28330"
     CDS             join(7557..7592,1..525)
                     /locus_tag="HVY77_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QMF70706.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            529..3495
                     /locus_tag="HVY77_28335"
     CDS             529..3495
                     /locus_tag="HVY77_28335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="QMF70707.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRGD
                     ANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRREHA
                     QELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALSVID
                     RACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKPNSRH
                     MLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYATLAAV
                     VLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIGQALLEA
                     KESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRYTPALLEV
                     LELRAAPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKFYE
                     ICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQYLE
                     ERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLPRI
                     KITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPGLTY
                     AKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRGESTG
                     HVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIEEHYTD
                     TAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTLNIKHVRAH
                     WDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVEL
                     RRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYL
                     ERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFRPLRMPGKP"
                     /name="Tn3-like element TnAs1 family transposase"
     gene            complement(4410..4643)
                     /locus_tag="HVY77_28340"
     CDS             complement(4410..4643)
                     /locus_tag="HVY77_28340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMF70708.1"
                     /translation="MTMLHLKTISNESIMLKKDDIILIKSYGNEKTKIFLRGVNEVLMI
                     DASFEEVKFAINNDLQDLQGMQAVSAPLKIAT"
                     /name="hypothetical protein"
     gene            complement(5670..7247)
                     /locus_tag="HVY77_28345"
     CDS             complement(5670..7247)
                     /locus_tag="HVY77_28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="QMF70709.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFTE
                     EELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPIAK
                     NGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSIISSA
                     MAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSLYTGR
                     TDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQAETDQ
                     IATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGEIRQATQ
                     VIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAA
                     RTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGIGQRVNEIT
                     DMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQLTSALSELA
                     KQFWEKRG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     Typing          5259..5474
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.908"
                     /coverage="100.0"
                     /evalue="7.28e-73"
ORIGIN
        1 agctttgacc agaacccgga acgccagctg gaacaaaccc aggtgagcaa ggtgttcacc
       61 gacaaggcat cgggcaagga cacccagcgc ccccagctcg aagcgctgct gagcttcgtc
      121 cgcgaaggcg atacagtggt ggtgcacagc atggatcggc tggcccgcaa cctcgatgac
      181 ctgcgtcgct tggtacagaa gctgactcag cgcggcgtgc gcatcgagtt cctgaaggag
      241 ggcctggtgt tcactggcga ggactcgccg atggccaacc tgatgctgtc ggtgatgggg
      301 gccttcgctg agttcgagcg cgccctgatc cgcgagcggc agcgtgaggg catcgccttg
      361 gccaagcagc gtggcgcgta ccggggccgc aagaaagccc tgtccgatga gcaggctgct
      421 accctgcggc agcgagcgac ggccggcgag cccaaggcgc agcttgcccg cgagttcaac
      481 atcagccggg aaaccctcta ccagtacctc cgcacggacg actgacacat gccgcgtcgc
      541 ttgatcctct cggccacgga gcgggacacc ctgcttgcgc tgccggaaag ccaggatgac
      601 ctgatccgct actacacctt caacgactcc gacctgtcgc tgatccgcca gcgacgcggc
      661 gacgccaacc gcctcggctt cgccgtgcag ctctgcctgc tgcgctaccc cggttacgcg
      721 ctgggaaccg acagcgagct gcccgagccg gtcatcctgt gggtggcgaa gcaagtccag
      781 gccgagccgg cgagctgggc aaagtacggc gagcgcgacg tgacccgtcg cgagcatgcc
      841 caggaactgc gcacctacct gcaactggcc ccgttcggcc tgtccgactt ccgcgccctg
      901 gtgcgcgagc taaccgagct ggcccagcag accgacaaag gcttgctgct ggccggtcag
      961 gccctggaga gcctacggca gaaacgacgc atcctgccgg cgctgagcgt gattgaccgg
     1021 gcctgctcgg aagccattgc gcgagccaat cggcgggtct accgcgccct ggtcgaacca
     1081 ctcacggact cgcatcgggc caagctggac gagctgttga agctcaaggc cggcagcagc
     1141 atcacctggt tgacctggct gcgccaggca ccgctgaaac ccaactctcg gcacatgctg
     1201 gaacacatcg agcggctgaa gacatttcag ttggtggact tgcccgaagg cctgggccgg
     1261 cacatccacc agaaccgcct gctcaagctg gcccgcgagg gtgggcagat gacgcccaaa
     1321 gacctcggta agttcgagcc gcagcgccgc tacgcgaccc tggccgccgt ggtgctggag
     1381 agcaccgcga ccgtgatcga tgagctggtc gatctgcatg accgcatcct ggtcaagctg
     1441 ttcagcggcg cgaagcacaa gcatcagcag cagttccaga agcagggcaa ggcgatcaac
     1501 gacaaggtgc gcctgtactc caggatcggc caggcgctgc tggaagcgaa ggaaagcggc
     1561 agcgacccct atgccgccat cgaggcggtg attccctggg acgagttcac cgagagcgtc
     1621 agcgaggccg agctgctggc ccggccggaa ggcttcgacc acctgcacct ggtcggcgag
     1681 aacttcgcca ccctgcgccg ttacacgccg gccttgctgg aggtgctgga actgcgcgcc
     1741 gcgccggccg cgcaaggcgt gctggcagcc gtgcagaccc tgcgtgagat gaacgccgac
     1801 aacctgcgca aggtgccggc cgatgcaccc acggccttca tcaagccgcg ctggaagccg
     1861 ctggtgatca ccccggaagg cctcgaccgg aaattctacg aaatctgcgc cctgtccgag
     1921 ctgaagaacg ccctgcgctc cggcgacatc tgggtcaagg gctcgcggca gttccgcgac
     1981 ttcgacgact acctgctgcc ggccgagaag ttcgccgcac tcaagcgcga gcaggccctg
     2041 cccctggcga tcaacccgaa cagcgaccag tacctggaag agcgtttgca gctgctggac
     2101 gagcagttgg ccaccgtcac ccgcctggcc aaggacaacg agctgcccga tgccatcctc
     2161 accgagtcag ggctgaaaat caccccgctg gatgcggcgg tgccggatcg ggcgcaggcg
     2221 ctgatcgacc aaaccagcca gttactgccg cgcatcaaga tcaccgaact gctgatggac
     2281 gtggacgact ggacgggctt cagccgccac ttcacccact tgaaggacgg ggccgaggcc
     2341 aaagacagga cgttgctgct gtccgcaatc ctcggtgatg cgatcaacct cgggctgacc
     2401 aagatggccg agtcgagccc cggcctgacc tacgccaagc tgtcctggct gcaagcctgg
     2461 cacatccgcg acgaaaccta ttcggcggcc ttggccgagc tggtcaacca ccagtatcgc
     2521 cacgcctttg ccgcccactg gggcgacggc acgacctcat cctccgatgg ccagcgcttc
     2581 cgcgcgggtg gccggggcga gagcaccggg cacgtcaacc cgaagtacgg tagcgagccg
     2641 ggacggctgt tctataccca tatctccgac cagtacgcgc cgttcagcac ccgcgtggtg
     2701 aatgtcggcg tccgcgattc cacctatgtg ctcgacggcc tgctgtacca cgagtccgac
     2761 ctgcggatcg aggagcacta caccgacacg gccggcttca ccgatcacgt ctttgccctg
     2821 atgcacctgc taggcttccg cttcgcgccg cgcatccgcg acctcggcga aaccaagctg
     2881 tacgtgccgc agggcgtgca agcctacccg acgttgcgcc cgctgatcgg cggcaccctg
     2941 aacatcaagc acgtgcgtgc ccactgggac gacatcctgc gcctggccag ctcgatcaag
     3001 cagggcaccg tcaccgcctc gctgatgctg cgcaagctcg gcagctaccc gcgccagaac
     3061 ggactggccg tggccctgcg cgagctgggc cggatcgagc gcacgctgtt catcctggac
     3121 tggctgcaaa gtgttgaact gcgccgccgc gtgcatgccg gcctgaacaa aggtgaggcg
     3181 cgcaactcgc tggccagggc ggtgttcttc aaccgccttg gggaaatcag ggatcggagc
     3241 ttcgagcagc agcgctaccg ggccagcggc ctcaacctgg tgacggcggc tatcgtgctg
     3301 tggaacacgg tgtacctgga acgcgccacc caggggttgg tcgaggccgg caagccggtg
     3361 gacggcgagc tgctgcaatt cctgtcgccg ctgggctggg agcacatcaa cctaaccggc
     3421 gattacgtct ggcggcagag ccgcagactg gaagacggga agtttcggcc cttacggatg
     3481 cccggaaaac cttagcgtac gattttttcc gaattctgcg ggctccccag tatgatgaac
     3541 aacgggatgc gaatgatgcg cagcttcccg gcgaatatga cctttttgat ttaatggcgc
     3601 aggttactga agagaacctg cacgccccgg tggattttgg gccgcctgtc ggcagggaag
     3661 cgttataact tttttcgctg gtcagcgtac gtgtaatacg ccggtattac atcttatttc
     3721 tttattttca tgtgattaca ggtggttgta atacgttgtg tattacataa ataaacggga
     3781 aattcagttt ttacctttta attcatgcaa ttgcgatgaa aatcgcggtt gcgtatggtg
     3841 tatgaccatt ttaaggttga aaaataatca actatggagg gtgatttacg caggttaagg
     3901 ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat gaacgggtta
     3961 ttcttcctga ctacagaatt acatcaaaag tacatcaaaa ttacatcact tttacgtcac
     4021 ttttacatca tttttacgtc agatctgacc tctgaactat tcgtacatcc gcccctcttc
     4081 ctgggcctcc tgcacgtgct gaaaatggtt tttccggctg tgccggcaac gcctcacggc
     4141 cacgcaataa attgcgcatc cgttcaccgt tatcggggtg gttcgtggtg gctttcaggg
     4201 cgttcatcgg attttagccg ctaaaatccc ggccaggtat gtctgtgcat agccatgcag
     4261 tcgcgccaga atcatcaggg gcgttttttc tcgatatggg cacagaaagg tgtgtgtttc
     4321 aggccggagg cctcagaaac gccctgaggg cctctcaggc ggtgcacgca acggatgtta
     4381 tggtaaatgt tgagtgctct tactaaaaat caggttgcta tttttagcgg tgcgcttact
     4441 gcctgcattc cctgaagatc ctgaaggtca ttattgatag caaactttac ctcttcaaag
     4501 ctcgcgtcaa tcattagaac ctcattcaca ccccgtaaaa atatttttgt tttttcgtta
     4561 ccatatgatt ttattaggat aatatcatct ttttttagca tgatactttc gttacttatt
     4621 gttttgagat gaagcatcgt catggttttt tttgcatgtg cttgttaagg ggattgagat
     4681 tattcctaac tccttgagtt tgtacataca aaaaaaacaa aaaaatccta atgcccccgt
     4741 ggtgggtata taaaaatcac ggggagcgca gcgaccttcg tccagctcgt ttcttgtgtg
     4801 tctcaccctg caaaaggttt gattgtgtgg gcgctgtgct gacgtctgag aatcacactt
     4861 tgccgcttac tgctgtctta ttcgctcatt ttatcaggtg cttgttatcg tacagcgaga
     4921 cggggtagag cctaactctt tcgcggttaa gttacgttcc caccaatttg cctgtttatc
     4981 ctgccggagt ccggggcgta gacctgagcg tctaaggtgc agatatgatt ttaatgtcgt
     5041 tgaatggtat taagctggta gtgacatcgc tagccagccc tgctaggatt tagattctca
     5101 cgtctgatcc tggcaggtat ctgcgaagaa ttagagtacc gaagccctgt gattctgtca
     5161 cggggctttt gtacatttag cgcccgagaa tcaaaaacat cccttcagaa cttatgtaac
     5221 gcccgccgca gtctcacggc aggagcgcag cgaccgagtg agcgaggagg cgacatttca
     5281 tccttacctg catgttgcac ttacgccccg cagctacatt tttcccctgc cacatgaagc
     5341 acttccctga attcccactt cgctttacca tattaagcca gtatacactc cgctagcgct
     5401 gcgtgactgg ttcagggctg cgccccgaaa cccgctaaca accccttagc tactggatat
     5461 caccccatgc catctgagta gggggaaccg cagaattcgg aaaaaatcgt acgctaagct
     5521 aacggtgttc tcgtgacagc tctttgacta ggctttctaa ggccattctg atagccctga
     5581 cttcctgaaa agccatggct aaaatttgtg cggctaaaag ggataaccga tggtaaagta
     5641 agttatccct gtcgagatac tgaaaagcgt tatcctcgtt tttcccaaaa ctgttttgcc
     5701 agttcgctca gagcgctagt taactgagcg acagatttcg cactttgcaa attattctgc
     5761 ccggtctgta cattggtatc agcagcatcg cgtatattga taatactgcg gtttatgtct
     5821 tcacttactg ctccctgctg ctcgaccgca gtcgctattt gcgcgttcat gtcggtaatt
     5881 tcgttaacgc gttggccaat tccatcaaga gctgtagctg cttcctctgc gtgagctaca
     5941 ctcgtgtgcg cttgccgact actttgctcc atgactgtaa cagcggattg cgctcgctct
     6001 tgtagagcgc tgatcatgct ttgaatatcc gttgtcgatt gctgtgtgcg agcagcaaga
     6061 ctgcgaacct catcggcgac aacagcaaaa ccacgcccct gctcaccagc acgcgcggcc
     6121 tcaattgctg cgttgagtgc caacaaattc gtttgctcgg cgatccctcg tataacgtca
     6181 agaacttttg atatctcgtt actttgacct tcaagctcat gaataacctg agtggcttgc
     6241 ctaatttcac cttcaagggc agtgattgac tggcttgtgt gggctaccag acgctggcca
     6301 gatgccgtct cagtgtctgc tcttccggcc gcatctgcag catgctgtgc attgctcgca
     6361 acctcttgaa tgcttgccac catttggttt actgccgttg ctatttgatc tgtctctgcc
     6421 tgctgctcaa ctgtaagtac attgcttgac tcaatatcct ttagtaggcc tcgggtgtgt
     6481 tcgctaagcc gatttgatgc atcacctatg cgacctacta tggcgcctgt ttcagcttgc
     6541 atcattcgta aagcaaactc tatttggcca aactcatcgg tgcgcccagt gtagagggat
     6601 tgacttaatg ggttattgga aatattcctg gctctttcaa ccagtcttcc aagaggagag
     6661 agaatagcca aaacactaac agagcttaag cttcctgaca ttaaagtggc taacaataag
     6721 ctgcttattg atgtatcagt aagcatgccg gcagccattg cgcttgatat aatactaccc
     6781 catatgagca agagtatttt cacggaaaag ctagcagcca atttcggcct cgcggccttc
     6841 ccgcttctca attgagcata taatttttcc gcagccaaaa cctgctcagg ttcaggcttg
     6901 gtccttacag actggtattc aacaatcgaa ccattcttag ctattggcgt tacataagca
     6961 cttacccaat agtggtcgcc atttttacag cgatttttta ctagccccat ccatgagcgg
     7021 ccagatttta atgtactcca catatgctca aatgcagcag gcggcatatc tgggtgtctt
     7081 acgatgttgt gaggctggcc taatagttct tcctcagtga aaccactgat tttaatgaag
     7141 tcaggattaa cgtacgtgat atggctttga ggggaggtag tcgaaagaat attggcatct
     7201 tttgggagtt ctaagtttcg acccgtcact ggtaaattct ggcgcataag aacctcaagg
     7261 gttggctgtt ttattttatt gttttcggca ttaagcccaa tttcttgagc gttacgataa
     7321 agctagcatg gaaacgatag gtgcaagcaa gttaagggtt gcatcgcgca tgtcaatcta
     7381 ggctataccc taacttgatg tcaggcaggg ccgcgccgct tcgtcagaat agagtctgct
     7441 ttcccatttt ttgacacatg cccgcgaagg ttatagattt cagcctgaca gaaatgggct
     7501 ttgaggcaca acggaacaga aagtgcactt aagccgcctt caaccaagga gacatcgtgc
     7561 aggggcaccg catcggctac gtccgggtca gc
//
