LOCUS       CP057228                2590 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB32-C18 plasmid pRHB32-C18_4, complete
            sequence.
ACCESSION   CP057228
VERSION     CP057228.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148321
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH13 T3-C18
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 152.268423951769x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 07:26:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,546
            CDSs (with protein)               :: 4,546
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 46 of 85
            Pseudo Genes (incomplete)         :: 39 of 85
            Pseudo Genes (internal stop)      :: 19 of 85
            Pseudo Genes (multiple problems)  :: 18 of 85
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB32-C18"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB32-C18_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(1310..2299)
                     /locus_tag="HVZ04_23740"
     CDS             complement(1310..2299)
                     /locus_tag="HVZ04_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QME83972.1"
                     /translation="MQNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYEHYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESD"
                     /name="protein rep"
     Typing          282..363
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="93.0"
                     /evalue="3.4000000000000004e-27"
     Typing          complement(843..1098)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.574"
                     /coverage="89.0"
                     /evalue="2.65e-108"
     Typing          complement(1224..1305)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.771"
                     /coverage="93.0"
                     /evalue="1.58e-25"
     Typing          1393..1584
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.446"
                     /coverage="100.0"
                     /evalue="2.86e-91"
ORIGIN
        1 gctggatttt cagtaaccaa aaacctccca accccaccca cttacaccca tctcatccac
       61 agactttcac cccgattttg tgaacaacaa aaaaccgtct tgcaggtggt tggcctcact
      121 ccgtgacacc aaccaccatc cccagtgttt tccccgtgcc gtcgtcaatc acaaaatcgg
      181 aacccacgcc agaggcgcgg ggccccgcga ttttctgttt gacgagttcg ggaacggtga
      241 gcggtacgcg gaattttttc gcgggccgtg aggcgttggc ggggtgtcgg ggcgcagccc
      301 tgaccaggtg gtaattgtga tagcgtcgcg tgtgacggta ttacaattac acatcctgtc
      361 ccgaaaattc ccgccgtttc tcggtttttt gagcgtcgca gaccgcacga caaacgaaac
      421 gaagtgaagt ttgtataagt gcgcacttcg tgagattccc tgactgccag tttgtcgatc
      481 acgcggaacg gctgcggcac aaaaacggca aaatgatttg ctgtccggtc gccagaattt
      541 ggcgcgtcgg tggcgatggc tgtacggaac gggaacgacc gcggggtggg gcgcgtggtg
      601 taaggacttg ttacgcgcac cgctaaaacg cgctgaacgc gattctgccg cgaacaccgc
      661 aaaaagggat gttttcccca gggtaaaccg gaaaaatgcg tcaggaacgt tttcagcgcg
      721 ttgcatgact atgcatgaaa ctgaatggcg atcggtttgg gcgcatacag tgcccatgag
      781 acatattttc ggacattttc aattcgtgaa tccctgactc attgctacat atctgaacag
      841 acacccctag cagcgccccc aggggcgtcc tgtaaaaaaa cacaccgcgc cgctagcagc
      901 acccctaata taaaattatg ttttttatga aaatagtcag taccacccct acaaagcggt
      961 gtcggcgcag ctacaacacc gcgccgacac cgctttttta aatatcacaa agagagcaag
     1021 agaaactaat ttgtcataac tctctattta taaagaaaaa tcagcaaaaa attgtttttg
     1081 cgtggggtgt gggcatttca ggcatgagaa cagactcgcg tttttccgga gtatcagcgg
     1141 aattcttgat taagaaacac tcgacgcaga gcgaaatttt atgcaacagc ggctgtactg
     1201 cacagggatt atttatcagt tgaaacagga caggatgtgt aattgtaata ccgtcacacg
     1261 cgacgctatc acaattacca cctggtcagg gctgcgcccc gacactctgc tagtctgatt
     1321 cagttttatc tttcgtcggt gagcggcggt actttctgac ctcggttttc cattcgaacg
     1381 cgatgcgtga accgtcatcg tcgccctcgg aaatatcatc tccgatcacc atgtcggcgt
     1441 cggtttcctg atcgagtttc agcacatctt tgagtgcgcc gccggtcgcc acaaagcggc
     1501 gcttgtgcgt ctggcgagtc agttcgagga accattcagg atcggccacc atgtcagccg
     1561 gcttcgtgga atatttcagg gtttcggcca ctgcgccgcg caccagttca gcggtcgcgc
     1621 aggctaagga ctcgccatcc ttcggcttgc ggggtttcac tgcccggaca tcaacattcg
     1681 ggtcataatc aacccgcagg cactcccgcc atagctcaac ccagcgttca tgcctgacgt
     1741 aacttttccc atttagcatg ccaggaggag ccatcatcaa ggtatggaag tgcgggtgtg
     1801 cgctgccgtc gctgccgcgg gtaacttccg ttgtgcggat ccagccctga acaggcagga
     1861 actccttgcg aaccttcata cgctgaaaag ctgcattcat ccggttcagc atctcgccca
     1921 gttccccaat cgcacagttg cgcacggtca gggtcaggaa catccagcgg gcatcgggat
     1981 aatccgccac gatacgcggc agggactggt agaaacgggc ttgccacatc agggagcgcc
     2041 tccactggca gacaggacag tgccggacac ggcagaaatg tgcctcacgc agcctcagac
     2101 gcgtttctcc ggtttccttg agggtagacc acccgaaacg cagtaaaccg ccacaggaag
     2161 ccatacgagc cccgtagtgc tcatattcgg ccgcaagcag gtaaatccct cccacgtcgt
     2221 cagaaacgct tctgtgtgcg tcccagggcg catcagaggg ggaataatca gtgaggtact
     2281 ggagtttagg gttttgcatg cacaccgccc catactgggc gatggctcga ttgacgtgtt
     2341 atcattgcca tatcacctgt tgctctcatc atgaatccag tttctgcagg tggtcacacc
     2401 cccgaacgat gcccgtcgtt gcgggggtca cttttaacgc aaccactccc gacagaataa
     2461 ctccaaaacc attttccggc aaatcccccc tcaggccttg cggcacaagg gctggaggcg
     2521 aatttgaatc ctagagttat cccttaagta taaatacaag gatcacgcgc gagatttgct
     2581 tgaattttga
//
