LOCUS       CP057183                1603 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB34-C04 plasmid pRHB34-C04_6, complete
            sequence.
ACCESSION   CP057183
VERSION     CP057183.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148337
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH03 T3-C04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 165.58193898008x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 07:49:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,916
            CDSs (total)                      :: 4,798
            Genes (coding)                    :: 4,549
            CDSs (with protein)               :: 4,549
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 249
            CDSs (without protein)            :: 249
            Pseudo Genes (ambiguous residues) :: 0 of 249
            Pseudo Genes (frameshifted)       :: 95 of 249
            Pseudo Genes (incomplete)         :: 154 of 249
            Pseudo Genes (internal stop)      :: 40 of 249
            Pseudo Genes (multiple problems)  :: 37 of 249
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB34-C04"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB34-C04_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            518..955
                     /locus_tag="HVZ20_24575"
     CDS             518..955
                     /locus_tag="HVZ20_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /protein_id="QME18871.1"
                     /translation="MNTQNLIAEHREKHDRDNLGFTQVYPLGWKRLMALAKGNQGAFGL
                     YTFLAQHIDGACGAVVADQAFLADQFDVTTRTVRNWISYLEEQKAIVKIPVAGRVCAYA
                     LNPHEVWKGYDNNKPTAAFLTKTLVSKDGEIKRRIMAMFSN"
                     /name="repA"
     Typing          31..111
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.341"
                     /coverage="87.0"
                     /evalue="2.0600000000000002e-29"
     Typing          complement(1259..1519)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.221"
                     /coverage="100.0"
                     /evalue="4.01e-96"
ORIGIN
        1 cacgtacgca ctgatgcggg gtgtcggggc gaagccctga ccagatggca aacggaatat
       61 cgtcgcgggt gacggtatta catttgcaca tcctgtcccg atttctgagg cgttttagcg
      121 gtgaacggac ggcaaaacca tgatggagct gttgaacgtt gagagcgtga aatctggcaa
      181 aaatcaccgc tggtaaggct tgcagaggag taagccagta tacactccgc tatcgctacg
      241 tgactgggtc agggcttcgc cccgacaccc caaaaaaacc aaaaccaaac gatcgtgcat
      301 taggaaacat agtgctctta agataacaat tagcagaaga tgtaagttat tgtttttatt
      361 tgtggaaata tagcatgata ggaaacacat gtgtttccgc atgataggaa atacatgtgt
      421 ttcctatcat ttctgaaagt aggaagtaaa taagcgttga tatggaaaat aaatttccta
      481 ttataggaat gataatttcc ggttgatagg aatcgctatg aatacccaga atctaatcgc
      541 agaacatcga gaaaaacatg acagagataa cctcggattc actcaagtgt atccactagg
      601 atggaagcgg ctgatggctc ttgccaaagg aaatcagggc gcatttggct tgtatacttt
      661 tttggcacag catatcgatg gggcgtgcgg tgctgttgta gcggatcagg cttttctggc
      721 tgatcagttt gatgtgacca ctcgaactgt acgcaattgg atctcgtacc tagaagagca
      781 gaaagccata gttaaaattc ccgttgcggg aagagtttgc gcctatgctc ttaacccaca
      841 cgaggtgtgg aaaggctatg acaataacaa gccaacagct gcattcttaa caaagacctt
      901 agtcagcaaa gatggggaaa ttaagcgccg gattatggcc atgttttcaa attgagccag
      961 ggcaggcgga caatcagggc tacgtgttaa cgttttgacc atgattgtct atcctgcatt
     1021 gctcttttcc cgacctcaaa ttcccttgca tattgaatgg gaattgcact acaaggtatt
     1081 tgagcgcgtt tcttcacagt cccttgaccc acatttgccc gtggtccctg aaacgcgctc
     1141 atcttccgtt ctgttccctg ccacggttcc ggcagcgcct tgagcgcaac cggaaaccgt
     1201 ctggggagaa ccccagaccc ccatcgctgt aacaccgacg gtctacgacc acctaccaac
     1261 cgcgtggctg cctgttccgt gcaaatgggc tttgcagcgg gctgacgcgg ttcctggcga
     1321 cctgaaagcc cctagttagg ggtatcggcg cgcctgacct cctgagactc tctgtaagcg
     1381 tttttcgcgc cttccatgcc ctgacgggca tttaaaaccc gtttcgtgcg tctgagggct
     1441 tctgagcgcg tttgaggggc tatctggcga ctcatccccg aaagggcaac agcagaatca
     1501 ccgacagcat gcgagaggtg gcaccgctcg ccgcagcccc agcgaccgaa cgttagtgag
     1561 cgagtgggcg aggaagcgga agagaaccgg aaaccacatt gag
//
