LOCUS       CP057122                3727 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB37-C15 plasmid pRHB37-C15_4, complete
            sequence.
ACCESSION   CP057122
VERSION     CP057122.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148379
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH15 T3-C15
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 156.462627016734x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 05:40:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,851
            CDSs (total)                      :: 4,732
            Genes (coding)                    :: 4,645
            CDSs (with protein)               :: 4,645
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 46 of 87
            Pseudo Genes (incomplete)         :: 34 of 87
            Pseudo Genes (internal stop)      :: 19 of 87
            Pseudo Genes (multiple problems)  :: 12 of 87
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB37-C15"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB37-C15_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            241..522
                     /locus_tag="HVZ62_24230"
     CDS             241..522
                     /locus_tag="HVZ62_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QMC99543.1"
                     /translation="MIKNIKHKGLRSFAEDGSTAGIQPHLAKKLRMILATLSTATCLQD
                     IVDVRALGCHPLKGDRKGEFSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(997..1110)
                     /locus_tag="HVZ62_24235"
     CDS             complement(997..1110)
                     /locus_tag="HVZ62_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMC99544.1"
                     /translation="MHEPPVQSDRCALSGNYHLESNPERHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            1680..1829
                     /locus_tag="HVZ62_24240"
     CDS             1680..1829
                     /locus_tag="HVZ62_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMC99545.1"
                     /translation="MPSEYDEQRDANDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            2213..2581
                     /locus_tag="HVZ62_24245"
     CDS             2213..2581
                     /locus_tag="HVZ62_24245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMC99546.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNDFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            complement(3004..3207)
                     /locus_tag="HVZ62_24250"
     CDS             complement(3004..3207)
                     /locus_tag="HVZ62_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017191052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMC99547.1"
                     /translation="MAIYVGPHNKIGRDGGSFQECDINTINHTVIRKYGRYCTIRDNEY
                     APPTSTPGRYWLQIYRTPDNTE"
                     /name="hypothetical protein"
     gene            complement(3335..3628)
                     /locus_tag="HVZ62_24255"
     CDS             complement(3335..3628)
                     /locus_tag="HVZ62_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="QMC99548.1"
                     /translation="MINLYDPVHPGEFIREMFMEPFEIPAAELADKLEVSQSALSRLLN
                     GKTDLSYEMALRLSKVIGRSAESWVNMQAQYSLSKSRKALSETLEHLHPMFP"
                     /name="HigA family addiction module antidote protein"
     Typing          1472..1645
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          complement(1002..1548)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.754"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 agcagtaagc tttaggatgc tctgtggagc agaaagctac cgctaatatg gtgtcataga
       61 ttgacttttt caaggcgcca tattgtatgg ttatcgctca tggaagtcaa acgaggtaat
      121 cgacctcaga gacaaactta cgtcaattat tttaataatg caaggggttc aaatggacgc
      181 tgttaaattg ttttctgttt atagagacag atatcgacga gatttttatg gagattatag
      241 atgataaaaa acataaaaca taaaggactt cggagctttg ctgaagacgg tagcaccgcc
      301 ggaattcagc cccatttagc gaaaaaactt cgcatgatat tggctacgct aagcaccgca
      361 acatgccttc aagatattgt ggatgttagg gcgttgggat gtcaccccct caaaggtgac
      421 cgtaaaggtg agttttccgt agaggttaac ggaaactgga gggtagtttt caaggtggaa
      481 gaacctaacg tcttagacgt cgaccttgaa gattatcatt agtaatcaaa aagtaagttg
      541 ctaatccatc ctgacctgaa tatggatgga ttagggactt tattcaaaaa aaagtccctc
      601 tggaggattt tatgagagcc tttatgattt tgttgttccc tttgctgtgt gtcgtcatcg
      661 caggggttct tgcagttcga gacttgaatg ggtgggggtg gttcttgttt gttgctgttt
      721 tgtgtactgg agctgatacg gtgcagaagc atgtttcctg caagaataat tccataagcg
      781 attgaggctt ttagggttcg tccttgactg atttaatcga caaagcgtat cttatgttcg
      841 aaacgaaaaa accgccctgc aaggcggttt ttcgaaggtt ctcagagcta ccaactcttt
      901 gaaccgcggt aactggcttg gaggagcgca gtaaccaaat ctgtcctttc agtttagcct
      961 taaccggcac ataacttcaa gactaactcc tctaaatcag ttaccagtgg ctgctgccag
     1021 tggcgctttt gcatgtcttt ccgggttgga ctcaagatga tagttaccgg acaaggcgca
     1081 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc
     1141 ggaactgagt gtcaggcgtg gaatgagata aacgcggcca taacagcgga atgacaccgg
     1201 taaaccaaaa ggcaggaaca ggagagcgca cgagggagcc actaggggga aacgcctggt
     1261 atctttaagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt
     1321 gtcagggggg cggagcctat ggaaaaacgg cttcgccgtg gccttattgc ttctccgtta
     1381 agcttcttct tggcatctcc caggaaattt ccgccccgcc tgtaagttgg taccgctcgc
     1441 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaata tatcctgtat
     1501 cacatattct gctgacgcac cggtgcagcc ttttttctcc tgccacatga agcacttcac
     1561 tgacaccctc atcagtgcca acatagtaag ccagtataca ctctgctagc gctacgtgac
     1621 tggttcaggg ctgcgccccg aaacccgcta acaacccctt agctactgga tatcacccca
     1681 tgccatctga gtatgatgaa caacgggatg cgaatgatac gcagcttccc ggcgaatatg
     1741 acctttttga tttaatggcg caggttactg aagagaacct gcacgccccg gtggattttg
     1801 ggccgcctgt cggcagggaa gcgttataac ttttttcgct ggtcagcgta cgtgtaatac
     1861 gccggtatta catcttattt ctttattttc atgtgattac agatggttgt aatacgttgt
     1921 gtattacata aataaacggg aaattcagtt tttacctttt aattcatgca attgcgatga
     1981 aaatcgcggt tgcgtatggt gtatggccat ttaagggata atgtaacctg acttttaaag
     2041 aatgaatcta tctttggcag cgcatgagat cgataaaaag cgttccacat caggcggttc
     2101 atggatacat gaaccggaaa ggacaacaat aagacaattg taagacattt tttgcgtttt
     2161 actcttattt ttggactatt atgagacaaa tgtaagacat tatgggcggt atatggcgaa
     2221 ttttaaccct ggtccaagaa cagccattgc agctgtcagg gattccaaga acgatataga
     2281 aacctggaag aaaaatggta tccgtaggga agagattgtg actgtgctga atgaacgtta
     2341 cggcctgaat atctcactgg ttgcgtttga taaggctctt tacagattga gaaaaagtac
     2401 agatgaaccg tctcttgctg atgtggattc tgtgtctgct gcagaaacga atttctctga
     2461 agccagtggg cctgacaatg atttttttct gtccgggaat gacttcatgc gagcagaaga
     2521 agaatctcaa agagtggttg aatcattcag agatagcctt agcaggaaga aaggattctg
     2581 attttgaatt ctaagtagtg aaatagtgga tcctctactc gaaattgtcg gatacctgca
     2641 attacctacc gtgccttatg gcttggtcaa tgcctcacgg ccacgcaata aattgcgtcc
     2701 ccgttcaccg ttttcggata agaagaacat cggaaacagg acttaccccg gctgaatggt
     2761 gtgaaattct gcgctatgca cttgcgcgca tactcatgca tgccgtaaaa acagagcctg
     2821 cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt taccggtttc ccgtcagaaa
     2881 cgccctgagg gcctctcagg cggtgcacgc aacggatgtt atggtaaata agagcgacag
     2941 tcctagtcac tcttaatgat agtcaccgta atgtggctgc tttcgctggg gatggatttt
     3001 tggttactcg gtgttatctg gggttcgata gatctgtagc cagtatcggc ctggtgtgct
     3061 tgttggtggt gcgtactcat tatctcgaat ggtgcagtat cgtccgtatt tgcggataac
     3121 agtatgattg atggtgttga tgtcacattc ttggaatgag ccaccatcac gacctatttt
     3181 gttgtgtggt cctacataaa ttgccatttt gttttccttt tcagggagtt aatgggcatc
     3241 ttatcggtgt ttaacaacaa tcacttctac atcaaatgaa cggtagatat gcggagcgtt
     3301 cgatggtgaa aagcccgcat tcgcgggctt ttacttatgg aaacatcgga tgcagatgct
     3361 ctagcgtctc ggataaagcc ttacggcttt tagagaggct gtactgagcc tgcatattaa
     3421 cccacgactc tgcagagcgg cctataactt tagatagtcg aagagccatc tcataactaa
     3481 gatcagtttt tccattgagc aatctgctta atgcagattg tgatacttca agcttatcag
     3541 ctaactctgc ggcaggtatt tcgaacggtt ccatgaacat ttccctgatg aactcaccgg
     3601 ggtgtacagg atcgtaaaga tttatcatgc cttcctcagt tggttttgta tttgattaca
     3661 caaaacattt tgttttcttt cgtattgcgc attgcacaat gtgcaagtgt ctgttattga
     3721 caaaaaa
//
