LOCUS       CP057005                8136 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB41-C03 plasmid pRHB41-C03_4, complete
            sequence.
ACCESSION   CP057005
VERSION     CP057005.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148421
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T3-C03
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 170.662648303445x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 03:11:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,437
            CDSs (total)                      :: 4,318
            Genes (coding)                    :: 4,056
            CDSs (with protein)               :: 4,056
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 119 of 262
            Pseudo Genes (incomplete)         :: 121 of 262
            Pseudo Genes (internal stop)      :: 46 of 262
            Pseudo Genes (multiple problems)  :: 22 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB41-C03"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB41-C03_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            327..695
                     /locus_tag="HV004_22130"
     CDS             327..695
                     /locus_tag="HV004_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMB34786.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            780..1763
                     /locus_tag="HV004_22135"
     CDS             780..1763
                     /locus_tag="HV004_22135"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="QMB34787.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENQGQSVARNVAIESSTGEYIWFLDGDDKISHGSLKLIDKI
                     TNEKNYGIIFFNAHVFFDTPDLKKKGWHDYKRSTFGEIDALTFFEKEMANKKLIVSPCC
                     YVFKRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEELFLRRVRNDSVMTSKK
                     SMRHIIGYSTCIDELTKYKKIKGIQNKSFDRFVNGMVLSLFSISETIKTKEAMNYVARY
                     KKHPGLSFKDSVSINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(2222..2925)
                     /locus_tag="HV004_22140"
                     /pseudo=""
     CDS             complement(2222..2925)
                     /locus_tag="HV004_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            3174..4628
                     /locus_tag="HV004_22145"
     CDS             3174..4628
                     /locus_tag="HV004_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="QMB34791.1"
                     /translation="MTALVSSFKWVGIGQLVKIATQLVSLFYLTHLIPPSSYGLLAMSS
                     VVMNLASIFNDMGTGAAVIQRQEASIGFYNYIYRINIFTGLFVMLLVIITSPLIVVYFN
                     TPELYPVLFLLAFSFPISSLTITHKAKLEKEMNFKVIIAIEIFSSLTGMVLAIVLANLG
                     FGVYSLIFQTLTTISISTICFLKVSGLSLSLRIKNISHSDKSGVIGFSGYLLSFNLINY
                     FSRNLDSILIGRFFSSAILGVYSIAYRIMLFPLQSLTLVFSRVFLPHLSKNINNTNNTN
                     KQDYLNALKLILSLSAPLMLGLSATSFSFTRIFFDPKWHLLGDLLHWLAPTAIIQSALS
                     TTGSVFMAYGKTRWLFFLGCIGAFLMGLSFIIGVHFDIKTLVYLYFFANLINSLPAFIL
                     LKNILKFSYIELVNVFFRTISPAFLMFLLIKVWQHFSFFKNASIDFSFNVALGIFSYIV
                     FYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(4721..4988)
                     /locus_tag="HV004_22150"
                     /pseudo=""
     CDS             complement(4721..4988)
                     /locus_tag="HV004_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(4960..5425)
                     /locus_tag="HV004_22155"
                     /pseudo=""
     CDS             complement(4960..5425)
                     /locus_tag="HV004_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(5624..5908)
                     /locus_tag="HV004_22160"
     CDS             complement(5624..5908)
                     /locus_tag="HV004_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QMB34788.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(6529..6846)
                     /locus_tag="HV004_22165"
     CDS             complement(6529..6846)
                     /locus_tag="HV004_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMB34789.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="hypothetical protein"
     gene            7931..8080
                     /locus_tag="HV004_22170"
     CDS             7931..8080
                     /locus_tag="HV004_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMB34790.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     Typing          7723..7896
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          complement(7248..7756)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ttttcatgta attacaggtg gttgtaatac gttgcgtatt acataaataa acggggactt
       61 cagtttttat cttttaattc atgcaattgc gatgaaaatc gcggttgcgt atggtgtatg
      121 accatttaag ggataatgta acctgacttt taaagaatga atctatcttt ggcagcgcat
      181 gagatagata aaaagcgttc cacatcaggc ggttcatgta tccatgaacc ggaaaggaca
      241 aaaataagac aattgtaaga cattttttgc gttttactct tattttggac tattatgaga
      301 caaatgtaag acattatggg cggtatatgg cgaattttaa ccctggtcca agaacagcca
      361 ttgcagctgt cagggattcc aagaacgata tagaaacctg gaagaaaaat ggtattcgta
      421 gggaagagat tgtgactgtg ctgaatgaac gttacggcct gaatatctca ctggttgcgt
      481 ttgataaggc tctttacaga ttgagaaaaa gtacagatga accgtctctt gctgatgtgg
      541 attctgtgtc tgctgcagaa acgaatttct ctgaagccag tgggcctgac aatggttttt
      601 ttctgtccgg gaatgacttc atgcgagcag aagaagaatc tcaaagagtg gttgagtcat
      661 tcagagatag ccttagcagg aagaaaggat tctgatttat tcaacaaagc cgttgataaa
      721 tcgcattcca taaataacaa cttgatgtta gtataactaa atattatttg gagattactg
      781 tgaaaaaaaa attttcgata atagtacctg tatataacgt tgaaaaatac ttaagggaat
      841 gtattcaatc agtattagat caggatttta acgactatga gataatctgt gtaaatgatg
      901 gttcaacaga tggcagttta gatatcatac tgtcatttga tgaccaaaga atcaaagtaa
      961 taaatcagga aaatcaaggc caatcggtgg caagaaatgt cgccattgaa tcatcaactg
     1021 gtgaatatat atggttttta gatggtgatg ataaaatatc acatggaagt ctaaagttaa
     1081 tagacaagat aaccaatgaa aaaaattatg ggattatttt ctttaatgca catgtttttt
     1141 ttgacactcc agatctgaaa aaaaaaggct ggcatgatta taagcgctca acatttggcg
     1201 aaatagatgc attaactttc tttgaaaaag aaatggcgaa caaaaaacta atagtgtcac
     1261 catgttgcta tgtttttaaa agggagttat taaatggcaa tgcattcatt ccgcatatat
     1321 atcatgagga taatcatttt atagcaaccc tgatttttga caattattca actatatgcc
     1381 tctcgttgga tgaggagtta tttcttagga gggtaagaaa tgattccgta atgacgtcta
     1441 aaaaatcaat gaggcacatc attggatatt caacatgcat cgatgagctt acgaagtata
     1501 aaaaaataaa aggcatacaa aacaagagct ttgatagatt tgtgaatgga atggttttgt
     1561 cgttattctc tatatcggaa acaataaaaa caaaggaagc aatgaactat gtggcgagat
     1621 ataaaaaaca ccccggccta tcatttaaag atagcgtatc aattaattac ccattaatta
     1681 aaaagactta tttaaaacta aaaaacggga aggtgtttaa ttacctaagg aatggcatta
     1741 ttaataaaaa aagcaaccct taataaaagc aaagcgataa ttaataaagg tttttctttg
     1801 ttgaataaat aagatttcgg ataggtatcc ccgtgacggg ttgggttttc aggcgatacg
     1861 cacgctttcc ggggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagatcg
     1921 gggtcagttt ggagatcgga aatttttgta cgttaagcgc attatttaac caatcttggt
     1981 tcgtaagggg ttgattttgc gtcagtggct atcgctttta agttgtcgct cttgcagtat
     2041 cgcatttagg ttatccgatt aaggtcaaac ctctgaaaat gtcgtatagc gcgggagtac
     2101 accctattgc tataggtaag tctgttcaaa aaacaggctt accgtacaat aattctctat
     2161 atccaaactg accccagatc acgcatcttc ccgacaacgc agaccgggta atgactccaa
     2221 cttactgata gtgttttatg ttcagataat gcccgatgac cttgtcatgc agctccaccg
     2281 attttgagaa cgacagtgac ttccgtccca gccttgccag atgttgtctc agattcagat
     2341 tcagattatg tcgctcaatg cgctgagtgt aacgcttgct gataacgtgc agctttccct
     2401 tcaggcgtga ttcatacagc ggccagccat ccgtcatcca taccacgacc tcaaaggccg
     2461 acagcaggct cagaagacgc tccagtgtgg ccagagtgcg ttcaccgaag acgtgcgcca
     2521 caaccgtcct tcgtatcctg tcatacgcgt aaaacagcca gcgctgacgt gatttagcac
     2581 cgacgtagcc ccactgttcg tccatttcag cgcagacaat cacatcactg cccggttgta
     2641 tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga cgtaaaaccg tgttgaggcc
     2701 aacgcccata atgcgtgcac tggcgcgaca tccgacgcca ttcatggcca tatcaatgat
     2761 tttctggtgc gtaccgggct gagaggcggt gtaagtgaac tgtagttgcc atgttttacg
     2821 gcaatgagag cagagatagc gctgatgtcc ggcagtgctt ttgccgttac gcaccacgcc
     2881 ttcagtagcg gagcaggaag gacatctgat ggaaatggaa gccacgcaag caccttaaaa
     2941 ttaccatcat acactaaatc agtaagttgg cagcattacc cactattggt ggagatgatc
     3001 ataatgcctt ataatcatac gggatacgaa cttataaagt cctaaagttt agctaaatcg
     3061 gaatctacaa cgtagatgat agcttgaaga attatgatgt cttttatggt ggtatcttta
     3121 aaaccatcac ggattataaa ttattgaaaa ttcaaaagga tattaaaaac aatatgactg
     3181 cactagtcag tagttttaaa tgggttggca ttggacaact tgtaaaaata gcaacacaat
     3241 tggtttcgtt attctacttg acgcacttaa tccccccatc gagctatgga ctattagcaa
     3301 tgtcatctgt ggttatgaat ttagcaagca tattcaatga catgggaact ggggcggcag
     3361 taattcagcg acaagaggcg tcgatcggtt tttataatta catatacagg ataaacattt
     3421 ttactgggtt gtttgttatg ctgcttgtaa taataacatc cccattaatt gtcgtgtact
     3481 ttaacacacc cgagctatac ccggttctat ttctattggc ttttagcttt ccaatttcca
     3541 gcttaacaat aactcacaaa gcaaaattag aaaaagaaat gaactttaaa gtcatcattg
     3601 caattgaaat tttctctagc cttacaggaa tggtgctggc aatagtttta gctaatttag
     3661 gctttggtgt ttatagtttg atttttcaaa cactgacaac aatatcaata tccacaatat
     3721 gttttttgaa ggtttccggg ttgagtttat cattgagaat taaaaatatc agccatagtg
     3781 acaagtcagg agttataggg tttagtggtt atttattatc ttttaattta ataaattatt
     3841 tttccaggaa tcttgattct attttaatag gccgtttttt ctcatccgca atacttgggg
     3901 tctattcgat tgcctaccgc attatgttat ttccgttgca gagcctgacg cttgtttttt
     3961 ctagagtttt cttaccacac cttagcaaaa acataaataa cactaataac actaataaac
     4021 aagattactt aaatgcatta aaacttatac tatcattaag cgcgccactg atgctaggtt
     4081 tatctgctac atctttttct tttacgagga ttttctttga tcctaagtgg cacctacttg
     4141 gagacttatt gcattggttg gcacctacag cgattatcca atcagccttg agtacaacag
     4201 gttctgtttt tatggcctac ggaaaaacaa ggtggttgtt tttcttaggt tgcattggcg
     4261 cttttttgat ggggttgtct ttcattatag gtgttcattt cgatataaaa acattggtct
     4321 acctttattt ttttgcaaac ttaattaatt cactcccggc atttatttta cttaagaata
     4381 tcttgaagtt tagttacatt gaattggtaa acgtgttttt tagaactatc tccccagctt
     4441 ttttgatgtt cttacttatt aaagtatggc agcatttttc ctttttcaaa aatgccagca
     4501 ttgatttttc ttttaatgtc gcattgggta tttttagtta tattgttttt tataccttgt
     4561 ttaatttcaa tgaggttgtt aggacaggag taattcataa gttaagatgc atgaccaagt
     4621 taaagtgatt catttattaa attcattaaa tgaattggta atgactccaa cttactgctt
     4681 gtgttttatg ttcagataat gcccgatacc gactgcggcc taattttttt aagtgtcgta
     4741 aaatcgtgtt gaggacagtg cccataatgc gggatgtggc ccggcatcca acgccattca
     4801 tggccatatc aatgattttc tggtgtgtac cgggctcaga ggcggtgtaa gtgaactgta
     4861 gttgcttgct atgttttacg acagtgagag cagagatagc gttgatgtcc ggtagtgctt
     4921 ttaccgttac gcaccacgcc ttcagtagct gaacaggagg gataaccagt ccagcgtgta
     4981 gagttcaggc tgccatacgg gcaattttcc agtgactgtg gttcggattc ttgcaaatta
     5041 gccccgaata ccccgtgtca gcgcccaatt ttttacgtag cgcgctctct acagcagcgg
     5101 cgtaacgtaa cggtttcatt tttccgtctg gcgcggtgcg tatcgaggtt ttcagtgcgt
     5161 acagcaggtg cgcgtgtccg ttttcggggt tggtgatggt cagtgtcggc gcgggggcgt
     5221 tgcggtcgct ccagtcaatg gctgcgccga tgcggtcaac gtcaaatccc agccagaaca
     5281 tggcgtgagg ctgattaaac tggatgtatt tagccagaat agcgcgctct ttccctgcga
     5341 tgcggacacc aaagtgtaaa tcatccgaga agtacggttt atgcggcaag cggtcgttga
     5401 aaagttcgag tgctgcgtta cgcaatgtgc cctgaactta tggcgggctg ttgtctggca
     5461 cggttcatgt gctaatatcc ttcttaggtt ttatacctag ccctgattta tcatgtctct
     5521 tccgccaagt tgaaatcatg attatcgggg ctttgttttt tctgttgccg ttaaagttac
     5581 acgcgaacgg cctcgcctgt cgaatttctt ctgtcacatc agcttaatgc gccgccagtt
     5641 catcaaaaaa ctttgggtta gcctgaatgg tggccagcac ttttgctgcc agtccggttg
     5701 ggttacggcg tccggtttcc cagcttttga tggtatcgag gctggttccc agcactttcg
     5761 ccatttcgct ctgagtaata ttcaggtttt ctctgatagc cctgacgtct gccagctcgt
     5821 agcgggtcac acgggcgggg gctttggttc cgtttttgat ttcaacggcc tcttccagag
     5881 aggctttcag ctcgtcaaaa aaactcatct tacacctcgt ctttcagtcg ttgggtcagc
     5941 ttcttcagtt ctgctttctc tgcgtctgtg atgctgtctt tggtgctttt agggtacgta
     6001 cagcatgtag acaacatgtc cgaaagtaag caaaaccatt aacggacagc cggatcattt
     6061 cgtacatccc ttacggtcag gggttcgcgc tcaatggaac gaaatcaccc gttagcttaa
     6121 aacgcttaac gtaggataat ttcctttttc caagcggacc cgctctactc gaaattgtcg
     6181 gatacctgca attacctacc gtgccttacg gcttggtcaa tgcctcacgg ccacgcaata
     6241 aattgcgtcc ccgttcaccg ttttcggata agaagaacat cggaaacagg acttaccccg
     6301 gctgaatggc gtgaatttcc gcgctatgca cttgcgcgca tactcatgca tgccgtaaaa
     6361 acagagcctg cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt taccggtttt
     6421 ccgtcagaaa cgcgctgagg gcctctgagg cggtgcggcc aacggatgtt atggtaaatt
     6481 cagctgattg gcaggtacaa aaaaagcccc gcagggcttt tttgtacctt attgatctgc
     6541 acactttgca gccttcaggg cttccagctg atttagccgg ttagctgctt ttttgcgggc
     6601 ttcgcctttt gccatgccat ttcctatccc aaaatcaccc aggaaaccta atacggtgcg
     6661 cccgtcaaat tcgccggttt cctcaatttc ttgttgtaca ctgcgcgttt tggcgatttc
     6721 cagagctacc gctttgcaat ccattgcgga agattctgga ccggtgacgt tcacggcctg
     6781 tggatattgc tttgttgcgc atcctgccat gatcagcgct gccgatagga tgaatatttt
     6841 tttcaaaatt atccttattt atttaggcat agttaagaga tacccatttt atcggcagga
     6901 aatgagggtt ttttagttga attttaggag gggcaaggtt ccatattggt cgaggattct
     6961 ggagctgacc ggcaacggcc ggcgcggcta tgggccatgc ggtggttgag catagaaagg
     7021 atcttcttga gttccttttt ttctacgcgt aatctcttgc tctgaaaacg aaaaaaccac
     7081 ctggggaggt ggtttttcga aggttaagtc agttggggaa ctgcttaacc tggtaactgg
     7141 cttcagcaga gcgcagatac caaatactgt ccttccagtg tagccgtagt taggccacca
     7201 cttcaagaac tctatagcac cacctacatt aactcgctct gctaatcctg ttaccagtgg
     7261 ctgctgccag tggcgctttg tcgtgtcttt ccgggttgga ctcaagacga tagttaccgg
     7321 ataaggcgca gcagtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa
     7381 ctgccttccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga
     7441 atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga
     7501 aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt
     7561 tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctatcg
     7621 cttccctgtt aagtatttcc ctggcatctt ccaggacatc tccgccccgt tcgtaagcca
     7681 tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat
     7741 atatcctgta tcacatattc tgctgacgca ccggtgccgc cttttttgtc ctgctacatg
     7801 aagcacttca ctgactccct catcagtgcc aacatagtaa gccagtatac actccgctag
     7861 cgctacgtga ctggttcagg gctgcgcccc gaaaccctct aacaacctct cagctactgg
     7921 atatcacccc atgccatctg aacatgatga acaactggat tcgactgata cgcagcttcc
     7981 cggcgaatat gacctttttg atttaatggc gcaggttact gaagagaacc tgcatgcccc
     8041 ggtggatttt gggccgcctg tcggccggga agcgttatag ctttttcgct tacccgcgta
     8101 cgtggtaata cgccggtatt acatcttatt tcttta
//
