LOCUS       CP057000                8136 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB41-C05 plasmid pRHB41-C05_4, complete
            sequence.
ACCESSION   CP057000
VERSION     CP057000.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148422
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T3-C05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 139.34638485268x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 03:15:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,441
            CDSs (total)                      :: 4,322
            Genes (coding)                    :: 4,056
            CDSs (with protein)               :: 4,056
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 119 of 266
            Pseudo Genes (incomplete)         :: 125 of 266
            Pseudo Genes (internal stop)      :: 46 of 266
            Pseudo Genes (multiple problems)  :: 22 of 266
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB41-C05"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB41-C05_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(7940..8136,1..1084)
                     /locus_tag="HV005_22150"
                     /pseudo=""
     CDS             join(7940..8136,1..1084)
                     /locus_tag="HV005_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(1351..1618)
                     /locus_tag="HV005_22155"
                     /pseudo=""
     CDS             complement(1351..1618)
                     /locus_tag="HV005_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1590..2055)
                     /locus_tag="HV005_22160"
                     /pseudo=""
     CDS             complement(1590..2055)
                     /locus_tag="HV005_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(2254..2538)
                     /locus_tag="HV005_22165"
     CDS             complement(2254..2538)
                     /locus_tag="HV005_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QMB30731.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3159..3476)
                     /locus_tag="HV005_22170"
     CDS             complement(3159..3476)
                     /locus_tag="HV005_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMB30732.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="hypothetical protein"
     gene            4561..4710
                     /locus_tag="HV005_22175"
     CDS             4561..4710
                     /locus_tag="HV005_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMB30733.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            5093..5461
                     /locus_tag="HV005_22180"
     CDS             5093..5461
                     /locus_tag="HV005_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMB30734.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            5546..6529
                     /locus_tag="HV005_22185"
     CDS             5546..6529
                     /locus_tag="HV005_22185"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="QMB30735.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENQGQSVARNVAIESSTGEYIWFLDGDDKISHGSLKLIDKI
                     TNEKNYGIIFFNAHVFFDTPDLKKKGWHDYKRSTFGEIDALTFFEKEMANKKLIVSPCC
                     YVFKRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEELFLRRVRNDSVMTSKK
                     SMRHIIGYSTCIDELTKYKKIKGIQNKSFDRFVNGMVLSLFSISETIKTKEAMNYVARY
                     KKHPGLSFKDSVSINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(6988..7691)
                     /locus_tag="HV005_22190"
                     /pseudo=""
     CDS             complement(6988..7691)
                     /locus_tag="HV005_22190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     Typing          4353..4526
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          complement(3878..4386)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 acaagaggcg tcgatcggtt tttataatta catatacagg ataaacattt ttactgggtt
       61 gtttgttatg ctgcttgtaa taataacatc cccattaatt gtcgtgtact ttaacacacc
      121 cgagctatac ccggttctat ttctattggc ttttagcttt ccaatttcca gcttaacaat
      181 aactcacaaa gcaaaattag aaaaagaaat gaactttaaa gtcatcattg caattgaaat
      241 tttctctagc cttacaggaa tggtgctggc aatagtttta gctaatttag gctttggtgt
      301 ttatagtttg atttttcaaa cactgacaac aatatcaata tccacaatat gttttttgaa
      361 ggtttccggg ttgagtttat cattgagaat taaaaatatc agccatagtg acaagtcagg
      421 agttataggg tttagtggtt atttattatc ttttaattta ataaattatt tttccaggaa
      481 tcttgattct attttaatag gccgtttttt ctcatccgca atacttgggg tctattcgat
      541 tgcctaccgc attatgttat ttccgttgca gagcctgacg cttgtttttt ctagagtttt
      601 cttaccacac cttagcaaaa acataaataa cactaataac actaataaac aagattactt
      661 aaatgcatta aaacttatac tatcattaag cgcgccactg atgctaggtt tatctgctac
      721 atctttttct tttacgagga ttttctttga tcctaagtgg cacctacttg gagacttatt
      781 gcattggttg gcacctacag cgattatcca atcagccttg agtacaacag gttctgtttt
      841 tatggcctac ggaaaaacaa ggtggttgtt tttcttaggt tgcattggcg cttttttgat
      901 ggggttgtct ttcattatag gtgttcattt cgatataaaa acattggtct acctttattt
      961 ttttgcaaac ttaattaatt cactcccggc atttatttta cttaagaata tcttgaagtt
     1021 tagttacatt gaattggtaa acgtgttttt tagaactatc tccccagctt ttttgatgtt
     1081 cttacttatt aaagtatggc agcatttttc ctttttcaaa aatgccagca ttgatttttc
     1141 ttttaatgtc gcattgggta tttttagtta tattgttttt tataccttgt ttaatttcaa
     1201 tgaggttgtt aggacaggag taattcataa gttaagatgc atgaccaagt taaagtgatt
     1261 catttattaa attcattaaa tgaattggta atgactccaa cttactgctt gtgttttatg
     1321 ttcagataat gcccgatacc gactgcggcc taattttttt aagtgtcgta aaatcgtgtt
     1381 gaggacagtg cccataatgc gggatgtggc ccggcatcca acgccattca tggccatatc
     1441 aatgattttc tggtgtgtac cgggctcaga ggcggtgtaa gtgaactgta gttgcttgct
     1501 atgttttacg acagtgagag cagagatagc gttgatgtcc ggtagtgctt ttaccgttac
     1561 gcaccacgcc ttcagtagct gaacaggagg gataaccagt ccagcgtgta gagttcaggc
     1621 tgccatacgg gcaattttcc agtgactgtg gttcggattc ttgcaaatta gccccgaata
     1681 ccccgtgtca gcgcccaatt ttttacgtag cgcgctctct acagcagcgg cgtaacgtaa
     1741 cggtttcatt tttccgtctg gcgcggtgcg tatcgaggtt ttcagtgcgt acagcaggtg
     1801 cgcgtgtccg ttttcggggt tggtgatggt cagtgtcggc gcgggggcgt tgcggtcgct
     1861 ccagtcaatg gctgcgccga tgcggtcaac gtcaaatccc agccagaaca tggcgtgagg
     1921 ctgattaaac tggatgtatt tagccagaat agcgcgctct ttccctgcga tgcggacacc
     1981 aaagtgtaaa tcatccgaga agtacggttt atgcggcaag cggtcgttga aaagttcgag
     2041 tgctgcgtta cgcaatgtgc cctgaactta tggcgggctg ttgtctggca cggttcatgt
     2101 gctaatatcc ttcttaggtt ttatacctag ccctgattta tcatgtctct tccgccaagt
     2161 tgaaatcatg attatcgggg ctttgttttt tctgttgccg ttaaagttac acgcgaacgg
     2221 cctcgcctgt cgaatttctt ctgtcacatc agcttaatgc gccgccagtt catcaaaaaa
     2281 ctttgggtta gcctgaatgg tggccagcac ttttgctgcc agtccggttg ggttacggcg
     2341 tccggtttcc cagcttttga tggtatcgag gctggttccc agcactttcg ccatttcgct
     2401 ctgagtaata ttcaggtttt ctctgatagc cctgacgtct gccagctcgt agcgggtcac
     2461 acgggcgggg gctttggttc cgtttttgat ttcaacggcc tcttccagag aggctttcag
     2521 ctcgtcaaaa aaactcatct tacacctcgt ctttcagtcg ttgggtcagc ttcttcagtt
     2581 ctgctttctc tgcgtctgtg atgctgtctt tggtgctttt agggtacgta cagcatgtag
     2641 acaacatgtc cgaaagtaag caaaaccatt aacggacagc cggatcattt cgtacatccc
     2701 ttacggtcag gggttcgcgc tcaatggaac gaaatcaccc gttagcttaa aacgcttaac
     2761 gtaggataat ttcctttttc caagcggacc cgctctactc gaaattgtcg gatacctgca
     2821 attacctacc gtgccttacg gcttggtcaa tgcctcacgg ccacgcaata aattgcgtcc
     2881 ccgttcaccg ttttcggata agaagaacat cggaaacagg acttaccccg gctgaatggc
     2941 gtgaatttcc gcgctatgca cttgcgcgca tactcatgca tgccgtaaaa acagagcctg
     3001 cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt taccggtttt ccgtcagaaa
     3061 cgcgctgagg gcctctgagg cggtgcggcc aacggatgtt atggtaaatt cagctgattg
     3121 gcaggtacaa aaaaagcccc gcagggcttt tttgtacctt attgatctgc acactttgca
     3181 gccttcaggg cttccagctg atttagccgg ttagctgctt ttttgcgggc ttcgcctttt
     3241 gccatgccat ttcctatccc aaaatcaccc aggaaaccta atacggtgcg cccgtcaaat
     3301 tcgccggttt cctcaatttc ttgttgtaca ctgcgcgttt tggcgatttc cagagctacc
     3361 gctttgcaat ccattgcgga agattctgga ccggtgacgt tcacggcctg tggatattgc
     3421 tttgttgcgc atcctgccat gatcagcgct gccgatagga tgaatatttt tttcaaaatt
     3481 atccttattt atttaggcat agttaagaga tacccatttt atcggcagga aatgagggtt
     3541 ttttagttga attttaggag gggcaaggtt ccatattggt cgaggattct ggagctgacc
     3601 ggcaacggcc ggcgcggcta tgggccatgc ggtggttgag catagaaagg atcttcttga
     3661 gttccttttt ttctacgcgt aatctcttgc tctgaaaacg aaaaaaccac ctggggaggt
     3721 ggtttttcga aggttaagtc agttggggaa ctgcttaacc tggtaactgg cttcagcaga
     3781 gcgcagatac caaatactgt ccttccagtg tagccgtagt taggccacca cttcaagaac
     3841 tctatagcac cacctacatt aactcgctct gctaatcctg ttaccagtgg ctgctgccag
     3901 tggcgctttg tcgtgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3961 gcagtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgccttccc
     4021 ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg
     4081 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt
     4141 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct
     4201 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctatcg cttccctgtt
     4261 aagtatttcc ctggcatctt ccaggacatc tccgccccgt tcgtaagcca tttccgctcg
     4321 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta
     4381 tcacatattc tgctgacgca ccggtgccgc cttttttgtc ctgctacatg aagcacttca
     4441 ctgactccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctacgtga
     4501 ctggttcagg gctgcgcccc gaaaccctct aacaacctct cagctactgg atatcacccc
     4561 atgccatctg aacatgatga acaactggat tcgactgata cgcagcttcc cggcgaatat
     4621 gacctttttg atttaatggc gcaggttact gaagagaacc tgcatgcccc ggtggatttt
     4681 gggccgcctg tcggccggga agcgttatag ctttttcgct tacccgcgta cgtggtaata
     4741 cgccggtatt acatcttatt tctttatttt catgtaatta caggtggttg taatacgttg
     4801 cgtattacat aaataaacgg ggacttcagt ttttatcttt taattcatgc aattgcgatg
     4861 aaaatcgcgg ttgcgtatgg tgtatgacca tttaagggat aatgtaacct gacttttaaa
     4921 gaatgaatct atctttggca gcgcatgaga tagataaaaa gcgttccaca tcaggcggtt
     4981 catgtatcca tgaaccggaa aggacaaaaa taagacaatt gtaagacatt ttttgcgttt
     5041 tactcttatt ttggactatt atgagacaaa tgtaagacat tatgggcggt atatggcgaa
     5101 ttttaaccct ggtccaagaa cagccattgc agctgtcagg gattccaaga acgatataga
     5161 aacctggaag aaaaatggta ttcgtaggga agagattgtg actgtgctga atgaacgtta
     5221 cggcctgaat atctcactgg ttgcgtttga taaggctctt tacagattga gaaaaagtac
     5281 agatgaaccg tctcttgctg atgtggattc tgtgtctgct gcagaaacga atttctctga
     5341 agccagtggg cctgacaatg gtttttttct gtccgggaat gacttcatgc gagcagaaga
     5401 agaatctcaa agagtggttg agtcattcag agatagcctt agcaggaaga aaggattctg
     5461 atttattcaa caaagccgtt gataaatcgc attccataaa taacaacttg atgttagtat
     5521 aactaaatat tatttggaga ttactgtgaa aaaaaaattt tcgataatag tacctgtata
     5581 taacgttgaa aaatacttaa gggaatgtat tcaatcagta ttagatcagg attttaacga
     5641 ctatgagata atctgtgtaa atgatggttc aacagatggc agtttagata tcatactgtc
     5701 atttgatgac caaagaatca aagtaataaa tcaggaaaat caaggccaat cggtggcaag
     5761 aaatgtcgcc attgaatcat caactggtga atatatatgg tttttagatg gtgatgataa
     5821 aatatcacat ggaagtctaa agttaataga caagataacc aatgaaaaaa attatgggat
     5881 tattttcttt aatgcacatg ttttttttga cactccagat ctgaaaaaaa aaggctggca
     5941 tgattataag cgctcaacat ttggcgaaat agatgcatta actttctttg aaaaagaaat
     6001 ggcgaacaaa aaactaatag tgtcaccatg ttgctatgtt tttaaaaggg agttattaaa
     6061 tggcaatgca ttcattccgc atatatatca tgaggataat cattttatag caaccctgat
     6121 ttttgacaat tattcaacta tatgcctctc gttggatgag gagttatttc ttaggagggt
     6181 aagaaatgat tccgtaatga cgtctaaaaa atcaatgagg cacatcattg gatattcaac
     6241 atgcatcgat gagcttacga agtataaaaa aataaaaggc atacaaaaca agagctttga
     6301 tagatttgtg aatggaatgg ttttgtcgtt attctctata tcggaaacaa taaaaacaaa
     6361 ggaagcaatg aactatgtgg cgagatataa aaaacacccc ggcctatcat ttaaagatag
     6421 cgtatcaatt aattacccat taattaaaaa gacttattta aaactaaaaa acgggaaggt
     6481 gtttaattac ctaaggaatg gcattattaa taaaaaaagc aacccttaat aaaagcaaag
     6541 cgataattaa taaaggtttt tctttgttga ataaataaga tttcggatag gtatccccgt
     6601 gacgggttgg gttttcaggc gatacgcacg ctttccgggg ctttgttgaa taaatcgaac
     6661 ttttgctgag ttgaaggatc agatcggggt cagtttggag atcggaaatt tttgtacgtt
     6721 aagcgcatta tttaaccaat cttggttcgt aaggggttga ttttgcgtca gtggctatcg
     6781 cttttaagtt gtcgctcttg cagtatcgca tttaggttat ccgattaagg tcaaacctct
     6841 gaaaatgtcg tatagcgcgg gagtacaccc tattgctata ggtaagtctg ttcaaaaaac
     6901 aggcttaccg tacaataatt ctctatatcc aaactgaccc cagatcacgc atcttcccga
     6961 caacgcagac cgggtaatga ctccaactta ctgatagtgt tttatgttca gataatgccc
     7021 gatgaccttg tcatgcagct ccaccgattt tgagaacgac agtgacttcc gtcccagcct
     7081 tgccagatgt tgtctcagat tcagattcag attatgtcgc tcaatgcgct gagtgtaacg
     7141 cttgctgata acgtgcagct ttcccttcag gcgtgattca tacagcggcc agccatccgt
     7201 catccatacc acgacctcaa aggccgacag caggctcaga agacgctcca gtgtggccag
     7261 agtgcgttca ccgaagacgt gcgccacaac cgtccttcgt atcctgtcat acgcgtaaaa
     7321 cagccagcgc tgacgtgatt tagcaccgac gtagccccac tgttcgtcca tttcagcgca
     7381 gacaatcaca tcactgcccg gttgtatgcg cgaggttacc gactgcggcc tgagtttttt
     7441 aagtgacgta aaaccgtgtt gaggccaacg cccataatgc gtgcactggc gcgacatccg
     7501 acgccattca tggccatatc aatgattttc tggtgcgtac cgggctgaga ggcggtgtaa
     7561 gtgaactgta gttgccatgt tttacggcaa tgagagcaga gatagcgctg atgtccggca
     7621 gtgcttttgc cgttacgcac cacgccttca gtagcggagc aggaaggaca tctgatggaa
     7681 atggaagcca cgcaagcacc ttaaaattac catcatacac taaatcagta agttggcagc
     7741 attacccact attggtggag atgatcataa tgccttataa tcatacggga tacgaactta
     7801 taaagtccta aagtttagct aaatcggaat ctacaacgta gatgatagct tgaagaatta
     7861 tgatgtcttt tatggtggta tctttaaaac catcacggat tataaattat tgaaaattca
     7921 aaaggatatt aaaaacaata tgactgcact agtcagtagt tttaaatggg ttggcattgg
     7981 acaacttgta aaaatagcaa cacaattggt ttcgttattc tacttgacgc acttaatccc
     8041 cccatcgagc tatggactat tagcaatgtc atctgtggtt atgaatttag caagcatatt
     8101 caatgacatg ggaactgggg cggcagtaat tcagcg
//
