LOCUS       CP056958                6715 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB41-C22 plasmid pRHB41-C22_4, complete
            sequence.
ACCESSION   CP056958
VERSION     CP056958.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148430
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T3-C22
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 147.446626226081x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 03:49:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,816
            CDSs (total)                      :: 4,694
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 52 of 136
            Pseudo Genes (incomplete)         :: 82 of 136
            Pseudo Genes (internal stop)      :: 22 of 136
            Pseudo Genes (multiple problems)  :: 20 of 136
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB41-C22"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB41-C22_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6493..6715,1..11)
                     /gene="mbeD"
                     /locus_tag="HV013_23995"
     CDS             join(6493..6715,1..11)
                     /gene="mbeD"
                     /locus_tag="HV013_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMA99497.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(386..592)
                     /locus_tag="HV013_24000"
     CDS             complement(386..592)
                     /locus_tag="HV013_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMA99491.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGKLMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(585..968)
                     /locus_tag="HV013_24005"
     CDS             complement(585..968)
                     /locus_tag="HV013_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMA99492.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKELI
                     SKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINHKI
                     SDTEDAVLSVGKIINLIEDSQHG"
                     /name="hypothetical protein"
     gene            complement(1359..2420)
                     /locus_tag="HV013_24010"
     CDS             complement(1359..2420)
                     /locus_tag="HV013_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="QMA99493.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMNP
                     IYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVIKG
                     NNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHEIRK
                     IFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKSPILD
                     QNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNINRIYT
                     EMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPERRFDQS
                     VYYTASHLFDEIK"
                     /name="HindVP family restriction endonuclease"
     gene            2508..3665
                     /locus_tag="HV013_24015"
     CDS             2508..3665
                     /locus_tag="HV013_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="QMA99494.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSHP
                     IKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKRIK
                     PKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGKLDV
                     RDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTVRGVN
                     RPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGNAVPVE
                     LAKYVAVTIMEYEKKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDSEG
                     VDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
                     /name="DNA cytosine methyltransferase"
     gene            complement(4711..4902)
                     /locus_tag="HV013_24020"
     CDS             complement(4711..4902)
                     /locus_tag="HV013_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMA99495.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGEDL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4967..5290
                     /locus_tag="HV013_24025"
     CDS             4967..5290
                     /locus_tag="HV013_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMA99498.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRSLRQVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5280..5990
                     /locus_tag="HV013_24030"
                     /pseudo=""
     CDS             5280..5990
                     /locus_tag="HV013_24030"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5968..6486
                     /locus_tag="HV013_24035"
     CDS             5968..6486
                     /locus_tag="HV013_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMA99496.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGMSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     Typing          4549..4633
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          complement(3980..4529)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.298"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4935..5284
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.162"
                     /coverage="99.0"
                     /evalue="1.96e-64"
     Typing          5281..6698
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.658"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 actggagata agagaggaac ggctgataca ggagcgcgaa catgcgttat ccctggaacg
       61 ggagcgtcag ccggaaatac aggaacgcat actggatggt ccttcgttgt cgctgtgatg
      121 gcgaaactat aaaaaatggc aggttcagtg gattttgacg gactaatgta gtctgcacca
      181 tctggttgca taggtattca tgcggttaaa atttatcagg cgcgatcgcg gcagtttttc
      241 gggtggtttg ttgccatttt tacctgtctg ctgccgtgat cgcaccgaac gcgttttagc
      301 ggtgtgcgta acggatgtta tggtaaatac aatatgcagc cagtcttaga gacgaaaagt
      361 ccgcccagaa gactgacttt tcagatcaac cttcagcatc taacttgtct atcgcttttt
      421 tattgcgccg ataggtagcc attagtttac catcgcctga ttgtaagtca cgacctgctt
      481 gctcagcata ggtatcgttt acagtcttag cgcctttttt ataggccttt ttaacatcat
      541 caaatccggc ttggatgact tctttcggct tttcttgagg taatttagcc atgctgtgaa
      601 tcctctataa ggttgattat ttttccaaca gataagacgg catcttctgt gtcgctgatt
      661 ttatgattta tccaaccgtc ctctatcttt aatttgttca ctactcctaa tgtaaaaaca
      721 atcttttttt caggaaagaa aattgcaaag gttctacttt tctctttcca atcagagggc
      781 ggcgtcgaac ggaacgctac attcctcgat ttaagtatcg ctttgatttt actaattaat
      841 tctttttttt gtttattgct gagatttttg acgaaccctt tgctgagatt gtctgtgtaa
      901 tcatcaaaag aaatatcaac tggtttgttt ttttgttttt tatcagatag atgaacaatg
      961 tttgacactg gatatcctgc tttttgatga atgaatactc agaagagtag cggaactgtc
     1021 ttgttagtca cagaggtaga gtatcaattc tacgatagat gggttcgaag atccggctga
     1081 gaccgttcag tacgcggtcg agatgttatg ttcaatgtaa tgaccgtcga aattgccttt
     1141 acgggcatga agcaaccagg agcaatcatt ggcaaaggta actagagttc aatgcatggt
     1201 aatcattttt gagcttgtga tgcatagagt tggttgctgt tccattgcgt ctcaaactcc
     1261 gtcaagatgc tcgccgtaaa agcgagcatg tctagtatgc ctgttccgct actgattttt
     1321 gatgggcgaa ttcagggctg gcgagccacc aagaaaaatt actttatttc atcaaacagg
     1381 tgagatgcag tataataaac gctttgatca aagcgtctct ctgggcttaa tttattttgc
     1441 ccgccattta aaattatttc ttttaatact gatggtggga ggatgggttt tgttcttctt
     1501 gggtgatcca tataagcttt tgttattttt ccgtttagcg agaactcttt atcactttga
     1561 tatccaaagt tcatctcagt ataaattcta ttgatgtttg tcttgttttt gctcgcaatt
     1621 tcatacacca ttctggcgag tctggctgat gatctcatta gtctgctggc agatgaattg
     1681 ttatatgagg atctatcaat aaaggttcga cacaaagcaa aatcactcca gatgaatatg
     1741 tcaaaggcat tttgatctaa tattgggctc ttgccttttg ttttccataa tggttgcatt
     1801 aaaaaaggtt tctggtaatc aagaaaattt tcttggaaag tgtttaggca atttaatata
     1861 ttttctttct ttgaaagaac ttcagtttca ttcccccagt gctgtatgct ttcgcatgtg
     1921 gattcaaata tttttcttat ttcatggaat ttatcgctgc aagagtgcat tatgccaagg
     1981 gcacaataag aagtgcttgc aggtcttatt acaatctctg aactccattc gctctcgttt
     2041 tctttatatg tcgtgctatc tggaagtacg gttaatttta tttctagtgc ttttaaaaaa
     2101 ctattgtttc cttttataac tagatctatt ccttttatgt catcataagc atactgttgg
     2161 tatggttcat attttttctc aaattgaaat tcaagatcac tattttttaa tgttgtgtta
     2221 aaaacgaagt caaaagatac ttctttggca tgcactttta aatccttatc taaataaaga
     2281 taaatcggat tcattttgtt gtctctcata taacatgcta gagaggtggg aaaagaactg
     2341 ttaaattggt tctttcccca cagatcgctc tctttcctgt tggtgttttt taaaccataa
     2401 agagattcta ttttgctcac tttttgctcc tgacgtcaat ttattgacgt ctatggtgtg
     2461 tttctatatc atgatgtgga gtgatatgat aatgagtttt ttatgttatg agaataattg
     2521 atctttttag tgggtgtggg gggttgtccc taggcttttt gaaggggggg tttgatgttg
     2581 taggtgctta tgatttctgg gatcctgcta ttgaatgtta cagagataat tttagccatc
     2641 cgataaaaaa actagatttg agcaatgttg atgatgtggt gagggagctt aaagatatag
     2701 acttcgatat gattattgga gggcctcctt gccaagactt cagtcatgca ggtttaagaa
     2761 ttgaaggtgc tagagctaac ttaactcgct cattttcaga aattataaaa agaataaagc
     2821 caaagtggtt tgtgatggaa aatgtagatc gggcattaag aagtggtgcc tatttagaag
     2881 ctcgaggaat ctttaaagaa tcagggtatg ggttaacaga aattgttttg gatgccagta
     2941 agtgtggtgt tccgcaaaaa agaaaaagat tatttgtaat tggaaagctt gacgtgaggg
     3001 atggttttat ccttaacgaa gtgatgtgcg ggatatcgaa agatagtatg acagttagaa
     3061 attatcttgg tgattcattg gggattgagt attattacag acatccgaga aattataaca
     3121 gaagggctat tttctctatt gatgaacctg cgccaacagt aagaggggtt aatagaccga
     3181 tacctgatgg atatttaggg catgcaggcg atcctgtcag tatttctgag aatgtaaggc
     3241 cattaacaac ctttgaaaga gcaagattgc aaacatttcc tgaagacttt aaatttaaag
     3301 gtgcaaaaac aaatttggag caaatgatag gtaatgctgt tccggttgag ttagcaaaat
     3361 atgttgcggt gacaatcatg gagtatgaaa aaaaacaagt aaaaggtatt tatgataaag
     3421 aaggctttag ggcgtggttg ctgaatgaaa agaaactaac aaaaagaacc tctagcgata
     3481 ttatatctag atgttgcaga ggtgtttcat tttttgattc agagggggtg gatttttata
     3541 actgtgaaat agatgaaatt attatgaaac tggaaaggct tgaatcgttt gtcaggcttg
     3601 gggttagcct gaagtctcag ttaagaaggg cttttaaatt atattacgaa tattgtcgaa
     3661 ggtaataaat acgaatttat gtctctcagg ggagggggag gggataaaaa gatcaaagga
     3721 tcctcttgtg atcctttttt tctgcgcgta atctgctgcc tgtaaacaaa aaaaccaccg
     3781 ctaccaacgg tggtttgttt gccggaatct agagctacca actctttctt ccgaaggtaa
     3841 ctggcttggt ggagcgcaga taccaaatac tgtccttcta gtgtagccgc agttaggcca
     3901 ccacttcaag aactcttaat atctcaatcc accttgtcca gttaccagtg gctgctgcca
     3961 gtggcgttaa gtcgtgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc
     4021 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4081 ccgaactgag ataccaacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4141 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4201 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4261 gtcgatttct gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcggcgcgg
     4321 ccttttgttg tcctttgcct ttttgctcac atgttctttc cggccttatc ccctgattct
     4381 gtggataacc gtattaccgc ctttgagtga gccgactccg ctcgccgcag ccgaacgacc
     4441 gagcgtagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     4501 acgcatctgt gcggtatttc acaccgcata tggtgctctt ttcttataat ccgcactgat
     4561 gccacatagt taagccagta tacactccgc tatcgctacg tgactggttc agggcttcgc
     4621 cccgaaaccc cctgacgcgc cctgacgggc ttgtctgctc ccggcatccg ctcacaggca
     4681 agctgtgacc gtctccggga gctgcatgtg tcagaggtct tcaccgtcat caccgaaacg
     4741 cgcgaggcag ctgcggtaaa gctcatcggc gtggtcgtga agagattcac aaatatcggc
     4801 ctgttcatct gcgtccagtt cgttgagttt ctccagcagc gttaatgtct ggcttctgat
     4861 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
     4921 gggatttctg ttcatggggg taatgatacc gatgaaacgc gagaggatgc tcacgatacg
     4981 ggttactgat gatgaacatg cccggttact ggaacgctgt gagggtaaac aactggcggt
     5041 atggatgcgg cgggtctgcc tgggtgagcc ggttgcccgt tccgggaagc tgccgacact
     5101 ggcaccgccg ttactgcgtc agctggccgc catcgggaat aacctgaatc agacagcgag
     5161 gaaggtaaac agcgggcagt ggtcttccgg tgaccgggtt caggtggtgg ccgcactgat
     5221 ggccatcgag ggagagttgc gcagcttacg gcaggtcgtc agggagcagg gagcgcggga
     5281 tgattgttaa atttcatgcc aggggaaaag gcggtggcag tggtccggtt gattacctgc
     5341 tgggaaggga gcgtaaccgg gaaggggcaa cggtgcttca gggtaatccg gaagaagtca
     5401 gggaactcat cgatgccacg ccatttgcga agaaatacac gtccggtgtt ctgtcgttcg
     5461 cggagaagga actgccgccg ggaggacgtg aaaaagtgat ggagagcttt gagcgcgtac
     5521 tgatgcccgg tctcgaaaaa aatcagtaca gcattctgtg ggtggagcac caggacaagg
     5581 ggcgtcttga gctgaatttt gtcattccga acatggagct acagaccgga aaacgcctcc
     5641 agccgtacta cgaccgagca gacaggccga gaattgatgc ctggcagaca ctggtgaatc
     5701 accattacgg gctgcatgac cccaacgccc cggagaaccg ccggatactg actctctctg
     5761 ataacctgcc ggaaacgaaa caggcgcttg ctgagagcgt cacacgcggg atagacgctc
     5821 tttatcatgt aggggagata aaaggccgtc aggatgtgat tcaggcgctc acagaggcgg
     5881 ggcttgaagt ggtcagggtg acgcgaagca gcatcagcat tgcagacccg aacggcggga
     5941 agaatatcag gctgaaagga gcgttttatg agcaatcttt tacagacggg cgcggagttc
     6001 gagaaaaagc tgaaagagag agccgaatct acagagaaaa tgctgaacga cgagttcagg
     6061 aagctcggcg agtctgtcag cgaggctgtg acctcaaacg agacgaaaat cagagacgct
     6121 atagccctgt tcacagcctc gacagagaaa tcgctggaaa aacacaggga aggggtgaaa
     6181 gaggcgatga tgcagcacag gaaggacgtg ttaaagctgg cagggaatac gggcatgatg
     6241 ttactgggga tagtctttct cctgtttacc gcgagtggcg ggacgctctg gtatcttggc
     6301 gggaggatac aggcgaacct ggaagaaatc aggaagcagg aagagacatt gcagaaactg
     6361 aacgcgaaga catggggcgt ggagtttgtg caggacggga gaaggaaatt tctggtcatt
     6421 ccgcaaggga tgtcagcaga ggtgacgcac tatcagggga aggactggat acaactgacg
     6481 gagtgatacg gaatgacaga gctggaaaca catttgctga acgccttaga gcagctgcaa
     6541 caggactata tgcagcggct gaacgaatgg gagagcgcct tcgtggaatt gcagaagatg
     6601 ttttcgctta cgcaacggga caacgcgatg ctgaacgagc gggtcatgca gttgagtcag
     6661 caggtgcagc acttgagcga gcggacagaa cgcttgagcc agttatacag cgaga
//
