LOCUS       CP056957                7941 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB41-C22 plasmid pRHB41-C22_3, complete
            sequence.
ACCESSION   CP056957
VERSION     CP056957.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148430
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7941)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T3-C22
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 147.446626226081x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 03:49:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,816
            CDSs (total)                      :: 4,694
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 52 of 136
            Pseudo Genes (incomplete)         :: 82 of 136
            Pseudo Genes (internal stop)      :: 22 of 136
            Pseudo Genes (multiple problems)  :: 20 of 136
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB41-C22"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB41-C22_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6510..7941,1..134))
                     /locus_tag="HV013_23940"
     CDS             complement(join(6510..7941,1..134))
                     /locus_tag="HV013_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /protein_id="QMA99481.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSSI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-10"
     gene            394..831
                     /locus_tag="HV013_23945"
     CDS             394..831
                     /locus_tag="HV013_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMA99482.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="entry exclusion protein 1"
     gene            912..1331
                     /gene="exc2"
                     /locus_tag="HV013_23950"
     CDS             912..1331
                     /gene="exc2"
                     /locus_tag="HV013_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMA99483.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(1894..2127)
                     /gene="mbeD"
                     /locus_tag="HV013_23955"
     CDS             complement(1894..2127)
                     /gene="mbeD"
                     /locus_tag="HV013_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMA99484.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(2134..2652)
                     /locus_tag="HV013_23960"
     CDS             complement(2134..2652)
                     /locus_tag="HV013_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMA99485.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            complement(2630..3376)
                     /locus_tag="HV013_23965"
                     /pseudo=""
     CDS             complement(2630..3376)
                     /locus_tag="HV013_23965"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3318..3641)
                     /locus_tag="HV013_23970"
     CDS             complement(3318..3641)
                     /locus_tag="HV013_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMA99490.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3706..3897
                     /locus_tag="HV013_23975"
     CDS             3706..3897
                     /locus_tag="HV013_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMA99486.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4992..5960
                     /locus_tag="HV013_23980"
     CDS             4992..5960
                     /locus_tag="HV013_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="QMA99487.1"
                     /translation="MKDQITHPPDNTDHSVAKQKFRITNWSTYNKALINRGSLTFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVPLR
                     CPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRIWRK
                     LHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRLSGSNARWKWTTEYNRRSIAETAMYRMK
                     QLLGDSLTLRDYDGQVAEAMAMVRALNRMTKAGMPESVRIA"
                     /name="IS5 family transposase"
     gene            complement(6005..6124)
                     /locus_tag="HV013_23985"
     CDS             complement(6005..6124)
                     /locus_tag="HV013_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMA99488.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSWLC"
                     /name="colicin release lysis protein"
     gene            6172..6513
                     /locus_tag="HV013_23990"
     CDS             6172..6513
                     /locus_tag="HV013_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="QMA99489.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin transporter"
     Typing          complement(3983..4070)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          4081..4639
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.606"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1791..3328)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.267"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttttgctac ctccagttcc accaccaccg ccagagccac tcccgtctgg attaccattc
       61 aaaagagtaa tgattacctg ccccttatca tcataaggaa caccatcttt atagtacgct
      121 acagctgttt ccattataaa atcctctttg acattaaaaa caaatagtta aaagcaatat
      181 actgtatata taatcactgg ttttatatac agtttaaaat tacgccgctg cattttccct
      241 gtcaaccctg tggattttca tttttgtgaa aacgatcaaa aaaacagcca cccacaccac
      301 gtgctatttc cacccgatgc cacaaaaacc agcacaaaca ttaccgttct cagacctcat
      361 tatgttttac tgaaaccatg agatgagaca cctatgggac actgtaactt tatggcatgg
      421 cacacactcc gggacgcact gaaaatgact ggaagatcgc gttcacagtt ttaccgtgat
      481 atgcgctcag gtcttgtcag ttaccgtacc agccaggacg gacgacggga gtttgaaacc
      541 agtgaactga tccgggcata cggcgaatta aagcggaatg agacaccaga aaggcacact
      601 caaggacact cagaaaaacc tcatgatcag cagacagaac gcattctccg ggaactgaat
      661 gagctgaaac aatgcctgac gctgatgctt gaggataaac aagcccagga tacggagcgc
      721 cggcgccagg aagcagaacg gaaacagcta cagaatgaga tagcccagct caggcaggct
      781 ctggagcagg aaaagaaacg gggattctgg tccaggctgt tcggtcactg aacgcgccca
      841 gaaactgatg ataagataat cttcggataa taactcaccg agaataaata ctttaaggta
      901 gggagacact catgagacgt accggaaaca aactttgtct tatcgccatg ataacagcaa
      961 cagtagctct cacagcctgt accccaaagg gcagcgtgga acaacatacc cggcattacg
     1021 tatatgcttc tgatgacggt tttgatccca acttttccac ccaaaaagcc gacacaacac
     1081 gaatgatggt gccttttttt cggcagttct gggatatggg agctaaagat aaagcgacag
     1141 gaaaatcacg gagtgatgtg caacaacgca ttcaacagtt tcacagccag gaatttttaa
     1201 actcactccg gggcacaact caatttgcgg gtactgatta ccgcagcaaa gaccttaccc
     1261 cgaaaaaatc caggctgctg gctgacacga tttctgcggt ttatctcgat ggctacgagg
     1321 gcagacagta agtggattta ccataatccc ttaattgtac gcaccgccga aacgcgttcg
     1381 gtgcgatcac ggcagcagac aggtaaaaat ggcaacaaac cacccggaaa actgccgcga
     1441 tcgcacccgg taaattttaa ccacatgcat acctatgcag ccaggtgaat cacgcaggaa
     1501 ttgccagtca gagaccgctg aaacggattt ttttcatact ttcggaagcg aagaagacgg
     1561 ggatttgagc aggaaaacag aagttcaggg gaatgagcgc gatctggcaa tagaggcaaa
     1621 aacacgacta tgaaagacac accagaatcg cgcccgtatg cgttttaacg cgttcctgtg
     1681 ttttcagcaa ggtttatgac tgaagcgcgt tcccggtgcc ccgaaccaac cattatccca
     1741 aaaaatgcac cggaaacgcg ctgacaacat tttagcctgt ctctgattac catcccagcg
     1801 aagggccatc cagtgtccgt tcctgtattt ccagctggcg ctcacgctcc agggataact
     1861 catgttcgcg ttcctgtatc agccgttcgt cccttatctc ctgttctcgc tgtataactg
     1921 gctcaagcgt tctgtccgct cgctcaagtg ctgcacctgc tgactcaact tcatgacccg
     1981 ctcgttcagc atcgcgttgt cccgttgcgt aagcgaaaac attttctgca attccacgaa
     2041 ggcgctctcc cattcgttca gccgctgcat atagtcctgt tgcagttgct ctaatgcgtt
     2101 cagcaaatgt ctttccagct ccgtcactct gtgtcactcc atcagttgta cccagtcctt
     2161 cccctgatgg ggaatcacct ctgctgattt cccgtacgga aggaccagaa atttcctgtt
     2221 cccgtcctgt acgaactcca cgccccatgt cttcgcgttc agtttctgca atgtctcttc
     2281 ctgcttcctg atatcttcca ggttcgcctg tatcatccct ccaagatacc agagcgtccc
     2341 gcaactcacg gtaaacagga aaatgaccat ccccagtaac atcatgcccg tattccctgc
     2401 cagctttaac acgtccttcc tgtgctgcat catcgcctct ttcacccctt cccggtgctt
     2461 tttcagtgat tcctctgtcg aagctgtgaa cagggctata gcgtctttga ttttcgtctc
     2521 gtttgatgtc acagccttgc ttacagattt ttcgagcttg ctgaactcgt cgttcagcat
     2581 tgtctctgta gattcggctc tctctttcag ctttttctcg aactccgcgc ccgtctgtaa
     2641 aagattgctc atagaatgct cctttcagtc tgatattctt cccgccgttc gggtctgcaa
     2701 tgctgatact gcttcgcgtc accctgacca cttcaatccc tgcttccgtg agcgcctgaa
     2761 tcacatcctg acggcctttt atctctcctg catggtaaag acgtgtgacg ccctcagcaa
     2821 acgcctgttt cgtttccggc aggttatcag ggagagtcag tatccggcgg ttctccgggg
     2881 cgttggggtc atgcagcccg taatggtgat tcaccagtgt ctgccaggca tcaattctcg
     2941 gcctgtctgc ccggtcgtag tacggctgga ggcgttttcc ggtctgtagc tccatattcg
     3001 gaatgacaaa attcagctca agccgtccct tgtcctggtg ctccacccac aggatgctgt
     3061 actgattttt ttcgagaccg ggcatcagta cacgctcaaa gctcgccatc actttttcac
     3121 gccctcccgg cggcagctcc ttctccgcga acgacagaac accggacgtg tatttcttcg
     3181 caaatggcgt ggcatcgatg agttcccgga cttcttccgg attaccctga agcaccgttg
     3241 ccccttcccg gttacgctcc cttcccagca ggtaatcaac cggaccactg ccaccacctt
     3301 ttcccctggc atgaaactta actatcatcc cgcgccccct gttccctgac ggccagacgc
     3361 aaccggcgca gctcatcccc gatggccatc agtgcggcca ccacctgaac ccggtcgccg
     3421 gaagaccact gtccgctgtt caccttacgg gctgtctggt tcaggttatt cccgatggct
     3481 gccagctgac gcagtaacgg cggtgccagt gtcggcagcc tcccggaacg ggcaaccggc
     3541 tcacccaggc agacccgccg catccatacc gccagttgtt taccctcaca gcgttccagt
     3601 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctcgcg tttcatcggt
     3661 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac taaacaggaa
     3721 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct gctggagaag
     3781 ctcaacgaac tggacgcgga tgaacaggca gatatctgtg aatcgcttca cgaccacgcc
     3841 gatgagcttt accgcagctg cctcgcgcgc ttcggggatg acagtgaaaa cctctgacat
     3901 atgcagctcc cggagacggt cacagcttgc ctgtgagcgg atgccgggag cagacaagcc
     3961 cgccagggcg cgtcagggtg ttttagcggg tttcggggcg cagccctgaa ccagtcacgt
     4021 agcgatagcg gggtgtatac tggcttaact atgcagcatc agtgcggatt gtatgaaaag
     4081 tgcaccatga ccagtgtgaa ataccgcaca gatgcgtaag gagaaaatgc acgttccggc
     4141 gcttttccgc ttcctcgctc actgactcgc tacgctcggt cgttcggctg cggcgagcgg
     4201 tactgactca ctcaaaaacg ataatacagt tatccacaga atccggggat aagaccggaa
     4261 agaacatgtg cgcaaaaagg caaagcccaa caaaaggcca cgccgatggc gtttttccat
     4321 aggctccgcc cccctgacga gcatcacaaa aaccgacgct caagtcagag gtggcgaaac
     4381 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4441 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4501 ctttctcata gctcacgctg ttggtatctc ggctcggtgt aggtcgttcg ctccaagctg
     4561 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4621 cttgagtcca acccggtaag acacgactta gcgccactgg cagcagccgt tggtaacttg
     4681 gttagcagag cgatgatggc gcaaatggtg caacagagtt cttgaagtag tggcccgact
     4741 acggctacac tggaaggaca gtatttggta tctgcgctct gctgaagcca gttacctcgg
     4801 ttaagcagtt ccccaactga cttaaccttc gatcaaaccg cctccccagg cggttttttc
     4861 gtttacaggc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4921 tctactgaac agagatcccc gtcaatttag aagaagatgg gctttgttga ataaatcgaa
     4981 cttttgctga gttgaaggat cagatcacgc atcctcccga caacacagac cattccgtgg
     5041 caaagcaaaa gttcagaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
     5101 gctccctcac tttctggctg gatgatgagg ccattcaggc ctggtatgag tcggcaacgc
     5161 cttcatcacg aggaaggccc cagcgctatt ctgatctcgc catcaccacc gttctggtga
     5221 ttaaacgcgt attccggctg accctgcggg ctgcgcaggg ttttattgat tccatttttg
     5281 ccctgatgaa cgttccgttg cgctgcccgg attacaccag tgtcagtaag cgggcaaagt
     5341 cggttaatgt cagtttcaaa acgtccaccc ggggtgaaat cgcacacctg gtgattgatt
     5401 ccaccgggct gaaggtcttt ggtgaaggcg aatggaaagt cagaaagcac ggcaaagagc
     5461 gccgtcgtat ctggcgaaag ttgcatcttg ctgttgacag caacacacat gaagttgtct
     5521 gtgcagacct gtcgctgaat aacgtcacgg actcagaagc cttcccgggc cttatccggc
     5581 agactcacag aaaaatcagg gcagccgcgg cagacggggc ttacgatacc cggctctgtc
     5641 acgatgaact gcgccgcaaa aaaatcagcg cgcttattcc tccccgaaaa ggagcaggtt
     5701 actggcccgg tgagtacgca gaccgcaacc gtgccgttgc taatcagcgg ctgagcggaa
     5761 gcaatgcacg gtggaaatgg acaacggaat ataaccgtcg ctcgatagcg gaaacggcaa
     5821 tgtacagaat gaagcagttg ttgggagatt cactgacgct gcgtgactac gatggtcagg
     5881 tagcggaagc tatggccatg gtgcgtgcgt tgaacaggat gacaaaggct gggatgccag
     5941 aaagcgtgcg tattgcctga aaatccagcc agctacaggg tcgttcgcac gaaatcttat
     6001 ttattcaaca aagccaagaa gatgatggag ccacagtccc tccctgaaca tcacgtatat
     6061 agttagcctg acatccaaca aggaggttta tagcgaatat tcccacaaaa aatcttttcc
     6121 tcataactcg atccttataa aatgaaaaga atatataacg aggtttaatt tatgagttta
     6181 agatactaca taaaaaatat tttgtttggc ctatactgcg cacttatata tatatacctt
     6241 ataacaaaaa acaacgaagg gtattatttc ctagcgtcag ataagatgct atacgcaata
     6301 gtgataagca ctattctatg cccatattca aaatatgcta ttgaacacat attttttaag
     6361 ttcataaaga aagatttttt cagaaaaaga aaaaacctaa ataatgcccc cgtggcaaaa
     6421 ttaaacctat ttatgctata caatctactt tgtttggtcc tagcaatccc atttggattg
     6481 ctaggactgt ttatatcaat aaagaataat taaataccca acacctcatt tatagtatta
     6541 agtttattct tatcaataaa ggcacataga atgcctgtaa caatagcaat cccccagata
     6601 cctaatgtag ttccagcaag cacactaaaa agtaaaacaa caacataact aactccagca
     6661 tctacagctt tcttctctaa tgtaagaaat agcggcttcc agtcacctgt atccttaatt
     6721 tttaggatat cagataccac atcatatcca aaagaaacat gtcccgtaat cttcaagtac
     6781 ttggcaaact gatctaaatg cttagcccag tcttcatact taaccgcctc cagtgcattg
     6841 aaaattgcat cacggtctgc tttgctgaat ttcttattta atacatcctt gtatttctca
     6901 aaagcagcta gagcttcatt cacattgctg attttcttac ctttagattt atcagcaagt
     6961 tcctgtgcca tttttgaata tttttcacca tatttttcag atagcgtttg ataaaagcta
     7021 actgttgcat ccacagcatc cttaatctgc gagttcagga gattagtctg tgctgttttc
     7081 aaattatctt cagcatcatg aatacgtgaa actgctgtac ttcgcttatt attagcctga
     7141 gaaatagcct cttggattcc atttatctca gagctaatct ggttaagacg cgtttctgat
     7201 gctgttacct gcttttgttt ttcctccatc tcatcaccag ctctcgcccg tagtctggtg
     7261 gcctcaaaaa aaggacgact ctgaagcgga tcattcgccc taggagataa aagtttaacc
     7321 ctttcatcca gttctttata tttagcagat gcctgatcca actcatttcg ttttccggac
     7381 agcgtattcg tttctgcatc acgggcatga atactggagc ttaaacgggt attgagcgta
     7441 ttaatctctt catccacttt cgccagctca gattgtgcag cagcaagatt tttttgtgcc
     7501 acctccacag cccgggcctc ttcactcaat gctgccaggc gtttctcttc atcctcagcc
     7561 agtttcaatt ggcgttctgt ttcagcctgc tccttctcta tctctttacg tcgttgttct
     7621 gcttcctgaa aagccttttc cgcagcttcc gcttctttac gggctttttc ttctgctttt
     7681 gcaaggcgca accgctctgc ttctgcctgc atcgctgcat tattggcatg agccaggtca
     7741 ataacctccg gatgagtgga attatggcga agcgcctcat tcacaatatc cttcagatgt
     7801 tgagtcagcg catcccggtt tgcttttgct ttagcctgtg cttctgcggc agcttttgct
     7861 cgggcagcct gttctgcctg cgttttcttc aattgagcag tagaccattt agctgtggca
     7921 tgaatggctg cagaactttc a
//
