LOCUS       CP056949                7198 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB42-C02 plasmid pRHB42-C02_3, complete
            sequence.
ACCESSION   CP056949
VERSION     CP056949.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148433
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T3-C02
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 162.772649593351x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 03:36:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,844
            CDSs (total)                      :: 4,724
            Genes (coding)                    :: 4,611
            CDSs (with protein)               :: 4,611
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 45 of 113
            Pseudo Genes (incomplete)         :: 67 of 113
            Pseudo Genes (internal stop)      :: 24 of 113
            Pseudo Genes (multiple problems)  :: 21 of 113
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB42-C02"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB42-C02_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(620..811)
                     /locus_tag="HV016_24170"
     CDS             complement(620..811)
                     /locus_tag="HV016_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMA90662.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            876..1199
                     /locus_tag="HV016_24175"
     CDS             876..1199
                     /locus_tag="HV016_24175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMA90663.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPLARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1357..1899
                     /locus_tag="HV016_24180"
                     /pseudo=""
     CDS             1357..1899
                     /locus_tag="HV016_24180"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1877..2395
                     /locus_tag="HV016_24185"
     CDS             1877..2395
                     /locus_tag="HV016_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMA90664.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            2402..2635
                     /gene="mbeD"
                     /locus_tag="HV016_24190"
     CDS             2402..2635
                     /gene="mbeD"
                     /locus_tag="HV016_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMA90665.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSRTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(2953..3372)
                     /gene="exc2"
                     /locus_tag="HV016_24195"
     CDS             complement(2953..3372)
                     /gene="exc2"
                     /locus_tag="HV016_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMA90666.1"
                     /translation="MRLTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(3453..3890)
                     /locus_tag="HV016_24200"
     CDS             complement(3453..3890)
                     /locus_tag="HV016_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMA90667.1"
                     /translation="MGHCNFMAWHTLQDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            4116..6005
                     /gene="cra"
                     /locus_tag="HV016_24205"
     CDS             4116..6005
                     /gene="cra"
                     /locus_tag="HV016_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin R"
                     /protein_id="QMA90670.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGVTSTQARQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLTPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKEMGRVTERIVDAINLALILNQTE
                     SRLSESQKNVDVANQIISDTVKAINDVNKKIAEKRNQQVSLTDLMNKKQKEVEDLKKIF
                     KNHSYHRIRDAQREYDDARNKYALLASDINALQAQVSGLTARKQQAEQNKAAAEKAKAD
                     AAAKAAAEKAAAEAKAKAEAEKARKEAEEKANDEKAVLTKASEIIISVGDKAGEYLGDK
                     YKVLSREIADNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDAIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYVMKANNVREKSIEGYQTGNWGPLMLEIESWVLSGIASAVAL
                     SFFSVAVGSLFITAGVSVTAVGLMGIIFAGFIGALIDDDFVDKLNNEIIRPAY"
                     /name="cra"
     gene            complement(6033..6557)
                     /gene="cri"
                     /locus_tag="HV016_24210"
     CDS             complement(6033..6557)
                     /gene="cri"
                     /locus_tag="HV016_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_089619593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R immunity protein"
                     /protein_id="QMA90668.1"
                     /translation="MQKEYTYNKDLNRMLFLITFAGIIPLIIIFITYTSNPNFYLIRII
                     FDNTQNIPSVISSYNPVMTKVMDIYGKSAPLLAFIAFIMLFKHTRTPKITDRKKIITAC
                     ILSPFLCIFYAYFFLWHNFELTTSGRPVRLMSENDLTLLIFYICLYYCSFFITYALCLQ
                     PVIAYKLWKER"
                     /name="cri"
     gene            6677..6817
                     /gene="crl"
                     /locus_tag="HV016_24215"
     CDS             6677..6817
                     /gene="crl"
                     /locus_tag="HV016_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="QMA90669.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSELTGIAV
                     Q"
                     /name="crl"
     Typing          448..535
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          complement(2..425)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.897"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(7040..7198)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.625"
                     /coverage="89.0"
                     /evalue="7.250000000000004e-66"
     Typing          1190..2739
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.611"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
       61 acggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      121 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      181 caggggggcg gagcctatgg aaaaacgcca gcggcgaggc ctttcgttgc cctttgcctt
      241 tttgctcaca tgttctttcc ggccttatcc cctgattctg tggataaccg tattactgcc
      301 tttgagtgag cttgcaccgc tcgccgcagc cgaacgaccg agcgtagcga gtcagtgagc
      361 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
      421 caccgtatat ggcgctgttt tcttacaatc cgcactgatg ctgcatatct aagccagtat
      481 acactccgct atcgctacgt gactgggtca gggctgcgcc ccgacacccg ctaaaacctg
      541 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc tcacagacaa gctgtgtccg
      601 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc cccgaaacgt gcgaggcagc
      661 tgcggtaaag ctcatcggcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg
      721 cgtccagttc gttgagcttt tccagcagcg ttaatgtctg gcttctgata aagcgcgcca
      781 tgttaagggc ggttttttcc tgtttagtca ctgatgcctc cgtgtaaggg gggatttctg
      841 ttcatggggt aatgataccg atgaaacgcg agaggatgct cacaatacgg gttactgatg
      901 atgaacatgc ccggttactt gaacgctgtg agggtaaaca actggcggta tggatgcggc
      961 gggtctgcct gggggagccg cttgcccgtt ccgggaaact gccgacactg gcaccgccgt
     1021 tactgcgtca gctggccgcc atcggaaata acctgaatca gacagcccgt aaggtgaaca
     1081 gcgggcagtg gtcttccggt gatcgggttc aggtggtggc cgcactgatg gccatcgggg
     1141 atgagctgcg ccggctgcgt ctggccgtca gggaacaggg ggcgcgggat gatagttaaa
     1201 tttcatgcca ggggaaaagg tggtggcagt ggtccggttg attacctgct ggggagggag
     1261 cgtaaccgcg aaggcgcaac ggtgcttcag ggtaatccgg aagaagtccg ggaactcatc
     1321 gatgccacgc catttgcgaa gaaatacacg tcaggcgttc tgtcgttcgc ggagaaggag
     1381 ctgccgccgg gaggacgtga aaaagtgatg gcgagctttg agcgcgtact gatgccaggt
     1441 ctcgaaaaga atcagtacag catcctgtgg gtggagcacc aggacaaggg acggcttgag
     1501 ctgaattttg tcattccgaa catggagcta cagaccggaa aacgtctcca gccgtactac
     1561 gaccgggcag acaggccgag aattgatgcc tggcagacgc tggtgaatca ccattacggg
     1621 ctgcatgacc ccaacgcccc ggagaaccgc cggatactga ctctctctga taacctgccg
     1681 gaaacgaaac aggcgcttgc tgagagcgtc acacgcggga tagacgctct ttatcatgta
     1741 ggggagataa aaggccgtca ggatgtgatt caggcgctca cagaggcggg gcttgaagtt
     1801 gtcagggtga cgcgaagcag catcagcatt gcagacccga acggcgggaa gaatatcagg
     1861 ctgaaaggag cattttatga gcaatctttt gcagacgggc gcggagttcg agaaaaagct
     1921 gaaagagaga gccgaatcta cagagaaagt gctgaacaac gagttcagga ggctcggcga
     1981 atctgtaagc gaggctgtga catcaaacga gacgaaaatc agagacgcta tagccctgtt
     2041 cacagcctcg acagaggaat cgctggagaa acaccgggaa ggggtgaaag aggcgatgag
     2101 gcagcacagg aaggacgtgt taaagctggc agggaatacg ggcatgatgt tactggggat
     2161 agtctttctc ctgtttaccg cgagtggcgg gacgctctgg tatcttggcg ggaggataca
     2221 ggcgaacctg gaagatatca ggaagcagga agagacattg cagaaactga acgcgaagac
     2281 atggggcgtg gagttcgtgc aggacgggaa caggaaattt ctggtccttc cgtacgggaa
     2341 atcagcagag gtgattccct ttcaggggaa agagtgggta catctgaagg agtgacacag
     2401 aatgacagag ctggaaacac atttgctgaa cgccttagag cagctgcaac aggactatat
     2461 gcagcggctg agcgaatggg agagcgcctt cgtggaattg cagaagatgt tttcgcgtac
     2521 gcaacgggac aacgcgatgc tgaacgagcg ggtcatgcag ttgagtcagc aggtgcagca
     2581 cttgagcgag cagacagaac gcttgagcca gttatacagc gagaactgga gataagagag
     2641 gaacggctga tacaggagcg cgaacatgcg ttatccctgg agcgtgagcg ccagctggaa
     2701 atacaggaac gcacactgga tggtccttcg ctgggatggt gaaactatga aaaatggcag
     2761 gttaagtgga ttaagacggg ctaatgtggt ctgtaccatc tggttgcata ggtattcatg
     2821 cggttaaaat ttatcaggcg cgatcgcggc agtttttcgg gtggtttgtt gccattttta
     2881 cctgtctgct gccgtgatcg cgctgaacgc gttttggcgg tgcgtacaat taagggatta
     2941 tggtaaatcc acttactgtc ttccctcgta gccatcgaga taaaccgcag aaatcgtgtc
     3001 agccagcagc ttggattttt tcggggtaag gtctttgctg cggtaatcag tacccgcaaa
     3061 ttgagttgtg ccccggagtg agtttaaaaa ttcttggctg tgaaactgct gaatgcgttg
     3121 ttgcacatca ctccgtgatt ttcctgtcgc tttatcttta gctcccatat cccagaactg
     3181 ccgaaaaaaa ggcaccatca ttcgtgttgt gtcggctttt tgggtggaaa agttgggatc
     3241 aaaaccgtca tcagaagcat atacgtaatg ccgggtatgt tgttccacgc tgccctttgg
     3301 ggtacaggct gtgagagcta ctgttgctgt tatcatggcg ataagacaaa gtttgtttcc
     3361 ggtaagtctc atgagtgtct ccctacctta aagtatttat tctcggtgag ttattatccg
     3421 aagactattt tatcatcagt ctctgacagc gttcagcgac cgaacaacct ggaccagaat
     3481 ccccgtttct tttccagttc cagtgcctgc ctgagctggg ctatctcatt ttgtagctgt
     3541 tcccgttctg cttcctggtg tctgcgatcc atatcctgtg cctgtttatc ctcaagcatc
     3601 agcgtcaggc attgtttcag ctcattcagt tcccggagaa tgcgttctgt ctgctgatca
     3661 tgtggatttt ctgcctgtcc cacagtgtgc ctttcttgtg tctcattttg ctttaattcg
     3721 ccgtatgccc ggatcagttc actggtttca aactcccttc gtccgtcctg gctggtgcgg
     3781 taactgacaa gacctgagcg catatcacgg taaaactgtg aacgcgatct tccagtcatt
     3841 ttcagtgcgt cctggagtgt gtgccatgcc ataaagttac agtgtcccat aggtgtctca
     3901 tctcatagtt tcagtaaaac ataatgaggt ctgagaacgg taatgtttgc gccggttttt
     3961 gtggtttccg gcgggaatag cgcgtggtgt gagagactgt ttttttgatc gttttcacaa
     4021 aaatggaagt ccacactctt gacagggaaa atgcagcggc gtagctttta tactgtacat
     4081 aaagccagtg gttatgtgta cagtgtttgt ttttaatggt ttgttttaaa agtcaaagag
     4141 gaatgaatat gccggggttt aattacggtg ggtatggtga cggtactggc tggagttctg
     4201 aaagtggtgg tccagctcca ggcggtggta tgcatggaaa tagtggaggc cagcgcggag
     4261 ataacgcaaa cagtagcaat tctgtgagtc agcagatttc tgccattcag aatgatcaga
     4321 aacttaaaca gaaagtggtg aatatgctga ttgctgcgag aaaaatgaat cctgaagcaa
     4381 aaatgattct gggaagtatt gctccttctg gtgttatgca ggtcaccatt gagggggtaa
     4441 cctccactca ggccaggcag cttggtttgg gagggctggt catgggatat aacgcttctg
     4501 gtgttattgg ggctgtgggc gaaatagaca ccggtcaccg ccttaatgca tcaggagcaa
     4561 gtactcccgg aagcgaaact tctgtggaga gttttgtgaa cggacagaaa ccagccggag
     4621 aatggcatgc tgtggcgaag gacagctgga caggggctgg tccagtgaat gtagggctgg
     4681 ttaataacgc catcaaaagt gtccggatta tcaaaaaagg ctatgtgacg ggcgttctca
     4741 cgccagaaga ggtgatgaat aaagcagagt acaaagcaat gcggcaggct ttcgattccc
     4801 ttccactggc aaaacaggga gaggctgtca gacagattgt tgctgcttgg agtctggcat
     4861 atcaggattt tccggtaaat ctgaaaaaag agatggggcg tgtcactgaa cggattgttg
     4921 atgctattaa cctggcgctc attcttaatc agacagagtc ccgtttgtcg gaatcacaaa
     4981 agaatgttga tgtagcgaac cagattatca gtgatacagt aaaagctatt aatgatgtaa
     5041 ataagaaaat agcagaaaaa cgaaatcagc aggtatcact gactgatttg atgaataaaa
     5101 aacaaaaaga ggtcgaagat cttaaaaaga tattcaaaaa tcactcttat caccgtattc
     5161 gtgatgccca gagggaatat gatgatgccc gaaataaata tgctttactg gccagtgata
     5221 ttaatgcgct gcaggcacag gtttccggac tgacggcaag gaaacagcag gcagaacaga
     5281 acaaggcagc agcagaaaaa gcaaaggcag atgcagcggc gaaagctgca gcagaaaaag
     5341 cggctgctga agcgaaagca aaggctgaag ctgaaaaagc cagaaaggaa gcagaggaaa
     5401 aagcaaatga cgagaaggct gttctgacaa aagccagtga aattatcatc agtgttggtg
     5461 ataaggctgg ggagtatctg ggtgataaat ataaagttct ttctcgtgag atagcagata
     5521 atatcaaaaa ctttcagggc aaaacgattc gtagttatga tgaggcgatg gcttctgtca
     5581 ataaactgat ggctaatcct gatcttaaaa ttaatgccgc cgacagggat gcaattgtga
     5641 atgcctggaa agcatttgat gcagaggata tggggaataa gtttgccgcg ctgggtaaga
     5701 cgtttaaagc cgcagattat gtgatgaagg caaacaacgt ccgggagaag agtatcgagg
     5761 ggtatcagac cggaaactgg gggccgctga tgctggagat tgagtcctgg gtattgagtg
     5821 gtattgcttc tgctgtggcg ctatcatttt tctcagtcgc tgttggttca ttatttatta
     5881 cagcgggcgt gtctgtaacg gctgtgggac ttatgggcat tatttttgct ggctttatcg
     5941 gtgcgctgat tgatgatgat tttgttgata agttaaataa tgaaattatt cgccctgcgt
     6001 attaattgag agtcgaaagg tagtcaggag gactaccttt ctttccagag tttatatgct
     6061 attactggtt ggaggcagag ggcgtaagtt atgaaaaaag agcaataata caggcagata
     6121 taaaagataa gcaaagtaag atcgttctca gacatcaatc gaacaggtct tcccgatgtt
     6181 gttaactcga agttatgcca caagaaaaaa taagcataaa aaatacacaa aaagggggac
     6241 agtatacaag ctgttatgat tttttttctg tctgttattt tgggggttct tgtgtgtttg
     6301 aatagcatga tgaaagctat aaatgctaat aatggtgcgc tcttaccgta gatatccatc
     6361 acctttgtca tcacagggtt atatgaagaa attactgaag gtatattttg agtattatcg
     6421 aaaatgatgc ggattaaata gaagttgggg tttgatgtat aagttataaa aataataatt
     6481 aatgggatta tacccgcgaa agtaatcaaa aaaagcatcc tgtttaaatc tttgttgtaa
     6541 gtgtactctt tttgcattgg tataatactc ccggtttttc attgttatta atttagttgt
     6601 tgatgtgtat aagcatatca tgctgtgcga gtaatggtca ccctaatatt aggggtatga
     6661 gttaatgagg ggatatatga aaatgatatt atcaggaggg atgcttgtct tgatggcttt
     6721 gctatcaggc tgccaggcta attatattcg cgatgttcag ggagggaccg tcgcaccatc
     6781 ctcctcttct gaactgacgg gaatcgcggt tcagtagaaa agatcaaagg atcttcttga
     6841 gatccttttt ttctgcgcgt aatcttctgc ttgcaaacaa aaaaaccacc gctaccaacg
     6901 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttggag
     6961 gagcacagtc accaaaacct gtcttttcag tttagcctta accggctcat aacttcaaga
     7021 ccaactcctc taaatcagtt accagtggct gctgccagtg gcgctttgtc gtgtcttacc
     7081 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7141 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata ccaacagc
//
