LOCUS       CP056942                7235 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB42-C04 plasmid pRHB42-C04_3, complete
            sequence.
ACCESSION   CP056942
VERSION     CP056942.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148434
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T3-C04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 151.53365995679x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 03:47:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,795
            CDSs (total)                      :: 4,673
            Genes (coding)                    :: 4,575
            CDSs (with protein)               :: 4,575
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 48 of 98
            Pseudo Genes (incomplete)         :: 46 of 98
            Pseudo Genes (internal stop)      :: 25 of 98
            Pseudo Genes (multiple problems)  :: 18 of 98
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB42-C04"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB42-C04_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(7154..7235,1..50)
                     /locus_tag="HV017_23880"
     CDS             join(7154..7235,1..50)
                     /locus_tag="HV017_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QMA86052.1"
                     /translation="MKIVIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1059..1250)
                     /locus_tag="HV017_23885"
     CDS             complement(1059..1250)
                     /locus_tag="HV017_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMA86043.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1314..1637
                     /locus_tag="HV017_23890"
     CDS             1314..1637
                     /locus_tag="HV017_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMA86044.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1777..2337
                     /locus_tag="HV017_23895"
                     /pseudo=""
     CDS             1777..2337
                     /locus_tag="HV017_23895"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2315..2845
                     /locus_tag="HV017_23900"
     CDS             2315..2845
                     /locus_tag="HV017_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMA86045.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTAALKELLERYQKTTVSTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            2852..3085
                     /gene="mbeD"
                     /locus_tag="HV017_23905"
     CDS             2852..3085
                     /gene="mbeD"
                     /locus_tag="HV017_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMA86046.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(3643..4065)
                     /gene="exc2"
                     /locus_tag="HV017_23910"
     CDS             complement(3643..4065)
                     /gene="exc2"
                     /locus_tag="HV017_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMA86047.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            4396..4674
                     /locus_tag="HV017_23915"
     CDS             4396..4674
                     /locus_tag="HV017_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMA86048.1"
                     /translation="MKKCVPVLLFGVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(4666..4956)
                     /locus_tag="HV017_23920"
     CDS             complement(4666..4956)
                     /locus_tag="HV017_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMA86049.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEYDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            5197..6843
                     /gene="cka"
                     /locus_tag="HV017_23925"
     CDS             5197..6843
                     /gene="cka"
                     /locus_tag="HV017_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="QMA86050.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTVPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKETEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     LQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVTYDEWAKHLEKIS
                     KALKVTGYLSFGNDVWDGTRKGLETGDWRPLFVTLEKSAVDFGVAKIVALMFSFIAGAP
                     LGFWGIAIITGIVSSYIGDDELNKLNELLGI"
                     /name="cka"
     gene            complement(6840..7130)
                     /locus_tag="HV017_23930"
     CDS             complement(6840..7130)
                     /locus_tag="HV017_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421186.1"
                     /note="Members of this family include Cki (colicin K
                     immunity protein) for the pore-forming toxin Cka (colicin K
                     activity protein), as well as immunity proteins from other
                     related colicin systems; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cki family colicin immunity protein"
                     /protein_id="QMA86051.1"
                     /translation="MYLKYYLHNLPESLLPWLLYLTLNYNNDISLSFIFFASIHVLLYP
                     YSKLTIFSFIQKTTNIKKEPWYSYNLFYLLYLAMAIPVGLPSFIYYTLKRN"
                     /name="Cki family colicin immunity protein"
     Typing          888..975
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(317..865)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.109"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1628..3189
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.929"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccgttgcacc atcttcgtct tctgaactga cggggatcgc ggttcagtag aaaagatcaa
       61 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcctgtaaa cgaaaaaacc
      121 gcctggggag gcggtttagt cgaaggttaa gtcagttggg tactgcttaa ccgggtaact
      181 ggctttagtg gagcacagat accaaatgct gtccttccag tgtagccgct ataaggcttc
      241 aacttcaaga gttctgcgta tctaaatcca ctatcccgtt accaatggct gctgccagtg
      301 gcgttaagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      361 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      421 aactgagata ccaacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      481 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      541 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      601 ggtttttgtg atgctcgtca ggggggcgga gcctatggaa aaagcgccac ctgcgcggcc
      661 ttttccccgg gctttgtctt gttgcacaca tgttctttcc ggcgttatcc cctgattctg
      721 tggataaccg tatcgccgct tttgagtgag cagacaccgc tcgccgcagc cgaacgaccg
      781 agcgtagcga gtcagtgagc gaggaagcgg aagagcaccg gtacctgcat gttctcctta
      841 cgcatctgtg cggcatttca caccgtacat ggtgcacttt tcatacaatc cgcactgatg
      901 ccgcatggct aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
      961 ccgacacccg ctaaaacccg ctgacgcgcc ctgacgggct tgtcggctcc cggagacgct
     1021 cacagacaag ctgtgagcgt ctccgggagc tacatgtgtc agtggttttc accgtcatcc
     1081 ccgaaacgtg cgaggcagct gcggtaaagc tcatcggcgt ggtcgtgaag cgattcacag
     1141 atgtctgcct gttcatccgc gtccagttcg ttgagctttt ccagcagcgt taatgtctgg
     1201 cttctgataa agcgcgccat gttaagggcg gttttttcct gtttagtcac tgatgcctcc
     1261 gtgtaagggg gatttctgtt catggggtaa tgataccgat gaaacgcgag aggatgctca
     1321 caatacgggt tactgatgat gaacatgccc ggttacttga acgctgtgag ggtaaacaac
     1381 tggcggtatg gatgcggcgg gtctgcctgg gggagccggt tgcccgttcc gggaaactgc
     1441 cgacactggc accgccgtta ctgcgtcagc tggccgccat cggaaataac ctgaatcaga
     1501 cagcccgtaa ggtgaacagc gggcagtggt cttccggtga ccgggttcag gtggtggccg
     1561 cactgatggc catcggggat gagctgcgcc ggctgcgtct ggccgtcagg gaacagggga
     1621 cgcgggatga tagttaagtt tcatgccagg agaaaaggtg gtggcagtgg tccggttgat
     1681 tacctgctgg gaagggagcg taaccgggaa ggggcaacgg tgcttcaggg taatccggaa
     1741 gaagtccggg aactcatcga tgccacgcca tttgcgaata aatacacgtc cggtgttctg
     1801 tcgttcgcgg agaaggagct gccgcttgga gggcgtgaaa aagtgatggc gagctttgag
     1861 cgtgtactga tgcccggtct cgaaaagaat cagtacagca tcctgtgggt ggagcaccag
     1921 gacaagggac ggcttgagct gaattttgtc attccgaaca tggagctaca gaccggaaaa
     1981 cgcctccagc cgtactacga ccgggcagac aggccgagaa ttgatgcctg gcagacgctg
     2041 gttaatcacc attacgggct gcatgacccc aacgccccgg agaaccgcag gacgctgaca
     2101 ctccctgata atctgccgga aacgaaacag gcgattgctg agggcgttac acgcgggata
     2161 gacgctcttt atcatgtcgg ggagataaaa ggccgtcagg atgtgattca ggcgctcacg
     2221 gaggcggggc ttgaagttgt cagggtgacg cgaagcagta tcagcattgc agacccgaac
     2281 ggcgggagga atatcagact gaaaggagcg ttttatgagc aatcttttgc agacgggcgc
     2341 ggagttcgag aaaaagctga aagagagagc cgaatctaca gagaaaatgc tgaacgacga
     2401 gttcaggaag ctcgaagaat ctgtaaacag ggaactgacc tcaaacgaat ctttaatcag
     2461 gaacgctatc aacgaccata cagcagccct gaaagaactt ctggagaggt atcagaaaac
     2521 gaccgtcagc acgatggaca cacactggca aaccgtgctg aaaatgagcg tgaaacgctg
     2581 gctgtggctg attatcgtct cagtactgat gtttgccacg accgggagtc tgctgtggta
     2641 ccagggcatg aagataaacg cgaatatgaa tattctccgc gagcagaaag agagtctgga
     2701 gaagctgaac gcgaagacat ggggcgtgcg ttatcacgag gacagcaacg gacgttttct
     2761 ggtgttgccg aaggggatga aagccgaaac gaactggacg aaggacaacg gcaaactgaa
     2821 cgcagtgaga ctggtgcagg agtgacagag catgacggag ctggaaagac atttgctgaa
     2881 cgcattagag caactgcaac aggactatat gcagcggctg aacgaatggg agagcgcctt
     2941 cgtggaattg cagaaaatgt tttcgcttac gcaacgggac aacgcgatgc tgaacgggcg
     3001 ggtcatgcag ttgagtcagc aggtgcagca cttgagcgag cggacagaac gcttgagcca
     3061 gttatacagc gagaacagga gataagggat gaacggctga tgcaggaacg cgaacatgag
     3121 ttatatctgg aacgggagcg ccagccggaa attcaggaac ggacactgga tggcccttcg
     3181 ctgggatggt aaccagagac aggctaaaat gttggcagcg cgtttccggt gcattttttg
     3241 ggataatggc tggttcgggg gcaccgggaa cgcgcttcag tcagagcttg ctgaaaacac
     3301 aggaacgcgt taaaacgcat acgggcgcga ttctggtgtg tctttgttgc tgtgttttgc
     3361 cgttattgcc agatcgcgct cattcccctg aatttctgtt tttccgctcc ggttccgtct
     3421 tctgcctttc cgaaagtatg aaaaaaatcc gtttcagcag tctctggctg gcaattcctg
     3481 cgtgattcac atggctgcat agctatgcat gtggttaaaa tttatcaggc gcgatcgcgg
     3541 cagtttttcg ggtggtttgt tgccattttt acctgtctgt tgccgtgatc gcgatgaacg
     3601 cattatggcg gtgcgtacaa ttaagggatt atggtaaatc ccttactgtc tgccctcgta
     3661 tccatcgaga taaaccgcag aaatggtgtc tgccattagt ttcatttttt tcggcgaagg
     3721 catatcgctg tttgtgtatt ttctgccagc aaaggttgtt gtgctccgga gtgagtgaaa
     3781 aaatttttct gagtggaatt cttttacacg ttgctgtgcc gtttctgctg atactccggc
     3841 tgctttgtct ttcatcccca tatcccggaa ctgctgaaag aacgggagca tcatacgtat
     3901 ggtgtctgct ttctgggtgt agaagttagg atcaaatcca tcatctgaag cataaacata
     3961 atgccgggta tgtcgttcca ggctggtctt tggtgtgcat cctgtaagag ctattgttgc
     4021 tgttatcaga ggtatcagaa gcagcctgac agcggcagat ctcataaaca tctccttgcg
     4081 tttttaatct tgatactcag ggggttatat ctccgaagag tatctcttat catggggttc
     4141 caagccggaa acctagattt ttccgcgacc agtaacggcg tgggttgtca cataaacgac
     4201 cgattgagtg acactcacag aaacaacgat ttcgacactg aattcgtcac ataacccgtc
     4261 acttattcaa tgtcatgcaa agctgtctgt gactcgcttt agtgacggaa ttttctggga
     4321 ttccggctta gacccccatc atacgtcggt ttactcccaa tgtgatttga tggtcaatga
     4381 gtcaggaata atcccgtgaa aaaatgtgtg ccagtcctgc tttttggtgt tctttctgct
     4441 tcggttgcca gtcatgcgga cagcaatgtt tttcaacaaa aggccgagaa gacaaaaaac
     4501 ggcattgttg cggatgttaa tgccagacag aaaatgttca gtaacagact gaagatacaa
     4561 cttcagatcg atgagttgag agctgagctg gctggcacca gagatgccca aaaaagagag
     4621 gatttgcgta aaaaactgga gtctctggag cggcaaaaga aatccttacg ctgatggggg
     4681 cgtattttcc ccggtttctt ccataacctt tttaccgttc agatatttac gggcgaggcg
     4741 aatgcgtctt gcctggtata ccgccgagta agcctgggtg ggattcacac cattactgat
     4801 ttcactgacc acatgacgct gatctgccag tgtcagcgtg taaacctgtt tctggatgag
     4861 gtcacgggct ttgtcatact catatccggc agcaacaagg gtgtcgagaa gtcgtttgtc
     4921 tgttttgttc agtttatggc ggggtgattt gctcacatcg ttctccgttt tttcatgatc
     4981 caacagtttt atatctcatc ctgatttgct ccggtgttct gtttcgacgt tctcaatgcc
     5041 tgcctcccca tagtgtgatc cttattgcaa aaatgaaaat ccatgctctt gacatggaca
     5101 atgctgagta gtaggttttc actgtacata aaaccagtgg ttatatgtac agtatgttgt
     5161 ttttaattta ttgttttaat tatcaaagag gaatttatgg ctaaagaact aagtggatat
     5221 ggcccaactg ctggtgagtc tatgggggga acaggggcga atctgaatca gcaaggtgga
     5281 aataataata gtaatagtgg ggttcactgg ggtggtggct ctgggcatgg taataatggt
     5341 ggtcagggaa attcaaattc atctggttct acatcaactg tgatgaaaac cggtgagtca
     5401 tatctgactc cgtggggtga tgttgtcatt aataatgatg gccttccggt gatgaatggt
     5461 attgttatga cagaagagaa ttcaactctg gttgataatc catttggagg ggtctccaga
     5521 gttcttaatt ctttaattag tgatatgcca tcactttttg cagaaagctc aggaaataac
     5581 aacaataaca ctgcttcagt taatactgta ccaacgaatg ctcaggtcag tgatatggat
     5641 aagagttcga aggtggtggg taatgtaatt aatgaaaaac aaaagcagaa gaataaaatt
     5701 gctacacaga ttagtgaaaa acagaagaaa atcgaggaga tgaaaaaagt attcaagcat
     5761 cattcttatc atggtatcac tgatcttgag agggatgttg atgagttgca aaaaaaatca
     5821 aatcagcttg atgcagatat tagcaaactt aattcttata aaaatacatt gcaaagtaaa
     5881 attggtgatg tgaataaaca gaaggaaact gaagaaaagg ctcgtgaaaa tgcagaggtc
     5941 gctgaacatg aaactcttaa tgaagagaaa caggcggtgg cggaagcaga gaagcgttta
     6001 gctgaggcga aagctgaact ggcgaaggct gaaagtgatt tacagagtaa gcaagctact
     6061 gtttccagag ttgcaggaga gcttgaaaac gctcagaaga gtgttgacgt gaaggtcacc
     6121 ggatttccgg gatggcgtga cgttcagaaa aaactacagc gtcagcttga agctaagcag
     6181 gctgagtact ccgcagttga aaatgaactt aagaatgctg ttagtttcag ggatggtaag
     6241 gccgctgagg ttaaagaggc agagcagaaa ttaaaagaag ctcaggatgc tcttgaaaag
     6301 agccagataa aagatgctgt tgatactatg gttggatttt atcaatatat aaccgaacaa
     6361 tatggagaaa aatacgcaaa aattgctcag gatttagcgg aaaagtctaa aggaaagaaa
     6421 attcaaggtg ttgatgaagc tctggcagca tttgaaaagt ataaaaatgt actggataag
     6481 aaattcagta aggttgatcg ggatgctatt tttaatgcgt tagagtctgt tacttatgat
     6541 gagtgggcaa agcatctaga aaagatctct aaagctctta aggttaccgg atatttgtct
     6601 ttcgggaatg atgtatggga tggtacccgg aagggattag aaacaggaga ctggaggcct
     6661 ttatttgtca ctctggagaa gagtgcggta gatttcggag tggcaaaaat tgtggcatta
     6721 atgttcagtt ttattgctgg agctccactt ggtttctggg ggattgctat tatcacaggt
     6781 attgtttctt cttacatagg ggatgatgag ttgaacaaac ttaatgagtt gttagggatt
     6841 tagtttctct ttagagtgta atatatgaaa cttggcaatc ctactgggat tgccattgct
     6901 aggtaaagta aataaaaaag gttgtatgag taccaaggct ctttttttat gtttgtcgtc
     6961 ttttgtataa aagaaaatat cgttagtttt gaatatgggt atagtaatac atgtattgat
     7021 gcaaaaaata taaaagatag agatatgtca ttattatagt taagtgttaa atataataac
     7081 catggtagaa gtgattcagg taaattatgt aggtagtatt ttagatacat aatttttctt
     7141 ttgttggagg tgtatgaaaa tagtaatttt tctaattaca atattattct tgtcaggatg
     7201 tcaagcaaac tatattcgtg atgtacaagg aggaa
//
