LOCUS       CP056931                6930 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB42-C20 plasmid pRHB42-C20_4, complete
            sequence.
ACCESSION   CP056931
VERSION     CP056931.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148439
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH12 T3-C20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 162.261147489259x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 03:14:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,990
            CDSs (total)                      :: 4,871
            Genes (coding)                    :: 4,781
            CDSs (with protein)               :: 4,781
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 49 of 90
            Pseudo Genes (incomplete)         :: 40 of 90
            Pseudo Genes (internal stop)      :: 19 of 90
            Pseudo Genes (multiple problems)  :: 18 of 90
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB42-C20"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB42-C20_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(866..1057)
                     /locus_tag="HV022_24870"
     CDS             complement(866..1057)
                     /locus_tag="HV022_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QMA72770.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1122..1445
                     /locus_tag="HV022_24875"
     CDS             1122..1445
                     /locus_tag="HV022_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QMA72779.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1603..2145
                     /locus_tag="HV022_24880"
                     /pseudo=""
     CDS             1603..2145
                     /locus_tag="HV022_24880"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2123..2641
                     /locus_tag="HV022_24885"
     CDS             2123..2641
                     /locus_tag="HV022_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QMA72771.1"
                     /translation="MSNLLQTGVEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMRLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            2648..2881
                     /gene="mbeD"
                     /locus_tag="HV022_24890"
     CDS             2648..2881
                     /gene="mbeD"
                     /locus_tag="HV022_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QMA72772.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(3201..3623)
                     /gene="exc2"
                     /locus_tag="HV022_24895"
     CDS             complement(3201..3623)
                     /gene="exc2"
                     /locus_tag="HV022_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QMA72773.1"
                     /translation="MRSAAVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFHTQKADTIRMMHPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEEFLQSLRTTTT
                     FAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            3745..4023
                     /locus_tag="HV022_24900"
     CDS             3745..4023
                     /locus_tag="HV022_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMA72774.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKSGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(4015..4305)
                     /locus_tag="HV022_24905"
     CDS             complement(4015..4305)
                     /locus_tag="HV022_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMA72775.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            4546..6045
                     /gene="csa"
                     /locus_tag="HV022_24910"
     CDS             4546..6045
                     /gene="csa"
                     /locus_tag="HV022_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QMA72776.1"
                     /translation="MAKELSGYGPTAGESVGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDNLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSITRTGVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     NQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGNAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRTDSDALANAWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNNEIIKPAH"
                     /name="csa"
     gene            complement(6073..6612)
                     /gene="csi"
                     /locus_tag="HV022_24915"
     CDS             complement(6073..6612)
                     /gene="csi"
                     /locus_tag="HV022_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QMA72777.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMANNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            6642..6797
                     /gene="csl"
                     /locus_tag="HV022_24920"
     CDS             6642..6797
                     /gene="csl"
                     /locus_tag="HV022_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QMA72778.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GSSGSL"
                     /name="csl"
     Typing          694..781
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          complement(125..671)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.171"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1436..2985
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.412"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcacagatac caaatgctgt cctttcagtg tagccgctat aaggcttcaa cttcaagagt
       61 tctgcgtatc taaatccact tttcagttac caatggctgc tgccagtggc gctttgtcgt
      121 gtcttaccgg gttggactcg agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      181 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccggg ccgagatacc
      241 aacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      301 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccgggg ggaaacgcct
      361 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      421 gctcgtcagg ggggcggagc ctatggaaaa acgccagcgg cgcggccttt ttttgtcctt
      481 tgccttttta ctcacatgtt ctttccggcc ttatcccctg attctgtgga taaccgtatt
      541 tccgcctttt agtgagccgg caccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      601 gtgagcgagg aagcggaaga gtgtcgtaag ttgcattttc tccttacgca tctgtgcggc
      661 atttcacacc gtccatggcg cacttttcat acaatccgca tcgatgccgc atagttaagc
      721 cagtatacac tccgctatcg ctacgtgact gggtcagggc tgcgccccga cacccgctaa
      781 aacctgctga cgcgccctga cgggcttgtc tgctcccggc atccgctcac aggcaagctg
      841 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatccccg aaacgtgcga
      901 ggcagctgcg gtaaagctca tcggcgtggt cgtgaagcga ttcacagatg tctgcctgtt
      961 catccgcgtc cagttcgttg agcttttcca gcagcgttaa tgtctggctt ctgataaagc
     1021 gggccatgtt aagggcggtt ttttcctgtt tagtcactga tgcctccgtg taagggggat
     1081 ttctgttcat gggggtaatg ataccgatga aacgcgagag gatgctcacg atacgggtta
     1141 ctgatgatga acatgcccgg ttactggaac gctgtgaggg taaacaactg gcggtatgga
     1201 tgcggcgggt ctgtctgggt gagccggttg cccgttccgg gaaactgccg acactggcac
     1261 cgccgttact gcgtcagctg gccgccatcg gaaataacct gaatcagaca gcccgtaagg
     1321 tgaacagcgg gcagtggtct tccggtgacc gggttcaggt ggtggccgca ctgatggcca
     1381 tcggggacga gctgcgccgg ctgcgtctgg ccgtcaggga acaggggacg cgggatgata
     1441 gttaaatttc atgccagggg aaaaggtggt ggcagtggtc cggttgatta cctgctgggg
     1501 agggagcgta accgcgaagg cgcaacggtg cttcagggta atccggaaga agtccgggaa
     1561 ctcatcgatg ccacgccatt tgcgaagaaa tacacgtcag gcgttctgtc gttcgcggag
     1621 aaggagctgc cgccgggagg acgtgaaaaa gtgatggcga gctttgagcg cgtactgatg
     1681 cccggtctcg aaaagaatca gtacagcatc ctgtgggtgg agcaccagga caagggacgg
     1741 cttgagctga attttgtcat tccgaacatg gagctacaga ccggaaaacg cctccagccg
     1801 tactacgacc gggcagacag gccgagaatt gatgcctggc agacgctggt gaatcaccat
     1861 tacgggctgc atgaccccaa cgccccggag aacagccgga tactgacact ccctgataac
     1921 ctgccggaaa cgaaacaggc gcttgcagag agcgtcacac gcgggataga cgctctttat
     1981 catgtcgggg agataagagg ccgtcaggat gtgattcagg cgctcacaga ggcagggctt
     2041 gaagtggtca gggtgacgcg aagcagtatc agcattgcag acccgaacgg cgggaagaat
     2101 atcaggctga aaggagcatt ttatgagcaa tcttttacag acgggcgtgg agttcgagaa
     2161 aaagctgaaa gagagagccg aatctacaga gaaaatgctg aacgacgaat tcaggaagct
     2221 cggcgaatct gtaagcgagg ctgtgacctc aaacgagacg aaaatcaaag gcgctatagc
     2281 ccagtttacg gcctcgacgg aggaatcgct gaaaaaacac cgggaagggg tgaaagaggc
     2341 gatgatgcag caccggaagg acatgttaaa gctggcaggg aatacgggca tgaggttgct
     2401 ggggatagtc tttctcctgt ttaccgcgag tggcgggacg ctctggtatc ttggcgggcg
     2461 gatacaggcg aacctggaag aaatcaggat acaggaagag acattgcaga aactgaacgc
     2521 gaagacatgg ggcgtggagt ttgtgcagga cgggagaagg aaatttctgg tccttccgta
     2581 cgggaaatcg gcaacggtga ttccctatca ggggaaggac tgggtacaac tgacggagtg
     2641 acacagagtg acggagctgg aaagacattt gctgaacgca ttagagcaac tgcaacagga
     2701 ctatatgcag cggctgagcg aatgggagag cgccttcgtg gaattgcaga aaatgttttc
     2761 gcttacgcaa cgggacaacg cgatgctgaa cgagcaggtc atgcagttga gtcagcaggt
     2821 gcagcacttg agcgagcgga cagaacgctt gagccagtta tacagcgaga acaggagata
     2881 agggacgaac ggctgataca ggaacgcgaa catgcgttat ccctggaacg ggagcgtcag
     2941 ccggaaatac aggaacgcac gctggatggt ccttcgctgg gatggtgaaa ccatgaaaaa
     3001 tggcagcttc agtggattaa gaggggttaa tgtggcctgt accatctggt tgcataggta
     3061 ttcatgcggt taaaatttat caggcgcgat cgcggcagtt tttcgggtgg tttgttgcca
     3121 tttttacctg tctgctgccg tgatcgcgct gaacgcgttt cagcggtgcg tacaataagg
     3181 gattatggta aatgtctccc ttactgcctg ccctcgtagc catcgagata aaccgcagaa
     3241 atcgtgtcag ccagcagctt cattttttcc ggtgatggca tatcactgtt cgtgtatttt
     3301 ctgcccgcaa aggtggttgt ggttctcagt gactgaagga attcttcaga gtggaattct
     3361 tttacacgtt gctgtgccgt ttctgctgat actccggctg ctttgtcttt catccccata
     3421 tcccggaact gctgaaagaa cgggtgcatc atacgtatgg tgtctgcttt ctgggtgtgg
     3481 aagttaggat caaatccatc atctgaagca taaacataat gccgggtatg tcgttccagg
     3541 ctggtctttg gtgtgcatcc tgtaagagct attgttgctg ttatcagagg tatcagaagc
     3601 agcctgacag cggcagatct cataaacatc tccttgcgtt tttaatcttg atactcaggg
     3661 agttatatct ccgaagagta tctcttatca tacgtcggtt tactcccaat gtgatttgat
     3721 ggtcaatgag tcaggaataa tcccgtgaaa aaatgtgtgc cagtcctgct ttttagtgtt
     3781 ctttctgctt cggttgccag tcatgcggac agcaatgttt ttcaacaaaa ggccgagaag
     3841 acaaaaagcg gcattgttgc ggatgttaat gccagacaga aaatgttcag taacagactg
     3901 aagatacaac ttcagatcga tgagttgaga gcagagctgg ctggcaccag agatgcccaa
     3961 aaaagagagg atttgcgtaa aaaactggcg tctctggagc ggcaaaagaa atccttacgc
     4021 tgatgggggc gtattttccc cggtttcttc cataaccttt ttaccgttca gatatttacg
     4081 ggcgaggcga atgcgtcttg cctggtatac cgccgagtaa gcctgggtgg gattcacacc
     4141 gttactgatt tcactgacca catgacgctg atcagccagt gtcagcgtgt aaacctgttt
     4201 ctggatgagg tcacgggctt tgtcatgctc atatccggca gcaaccaggg tgtcgagaag
     4261 tcgcttgtct gttttgttca gtttatggcg gggagatttg ctcacatcgt tctccgtttt
     4321 ttcatgatcc aacagtttta tatctcatcc tgatttgctc cggtgttctg tttcgacgtt
     4381 ctcaatgtct gcctccccat agtgtgatcc ttattgcaaa aatgaaaatc catgctcttg
     4441 acatggataa tgctgagtag taggtttttg ctgaatacaa aaccagtggt tatatgtaca
     4501 gtatattgct tttaatttat tgttttaatt atcaaagagg aatttatggc taaagaacta
     4561 agtggatatg gcccaactgc tggtgagtct gtggggggaa caggggcgaa tctgaatcag
     4621 caaggtggaa ataataatag taatagtggg gttcactggg gtggtggttc aggctctggt
     4681 aatggaggtg gtgaacatgg aagtcagacg gggtggggat ggagtaaaac caataatccg
     4741 gatgtgccgc cttatgttga tgataatggg caggtgcgca ttaccatcac aaatggtctg
     4801 gtaaaaacac ctgtatatgg agttcctggg gctggcggga atagtgatgt tcaggggggc
     4861 tatattcctg aaaatcctaa tgatgaagtt gctcgtaagt gggataaaaa caacttaccg
     4921 cgagagattg atgtgagtat cgatggtttt aaatatcgtg taacgcttaa tgataatggt
     4981 agagctatcg gtattcttcg aacaggtgtc cgtccttacg tagggagtga aaaagctaag
     5041 gcgggtatta tggaaaaaat taatcataaa acacctgagg agatatatga ggcgttaggt
     5101 tttaataaag atgaatctca gcggcaggaa aaagcaaaac aacaggctga agatgcgtgg
     5161 gataatttgc ctcctaatgt tagaaaattt gatgttgatg ttgaacagtt ccactattta
     5221 gtggtgcttg atgattatgg taatgtttta tctataacta gaacaggtgt tcgtccttat
     5281 gtagggagtg aaaaagctaa ggctggtatt atggataaag tcgaccataa gaccccggaa
     5341 gagatatatg aggcattagg atttaataat gaagagcctc aacgacagaa tcaagctaaa
     5401 aaagctgctt atgatgtgtt ttattctttc agtatgaacc gtgaccggat tcagagtgat
     5461 gtacttaata aagctgctga agttatctct gatataggta ataaggtcgg tgattatctt
     5521 ggaaatgctt ataagtcgtt agcaagagaa attgctgatg atgtcaaaaa tttccagggg
     5581 aaaaccatcc gtagctacga tgatgcaatg gcgtcattaa ataaagtttt gtctaatcct
     5641 gggtttaaat ttaacaggac cgacagtgat gcactggcga acgcctggcg aagtattgat
     5701 gctcaggata tggcaaataa actgggtaat atcagcaagg ctttcaaatt tgccgatgtg
     5761 gtgatgaagg ttgagaaggt ccgggagaaa agcatcgagg gctatgaaac cgggaactgg
     5821 ggacctctga tgctggaggt ggagtcctgg gtgctcagtg gcattgcttc tgctgtggcc
     5881 ctgggggttt tttcagccac attaggggca tatgccttat ctcttggggc tcctgctatt
     5941 gctgtcggta tcgtaggtat tctactcgca gcagttgtag gtgcattaat tgatgacaag
     6001 tttgcagatg cactaaataa cgaaatcatt aaacccgctc attaataaat accgggtaaa
     6061 aggaaggcag ggttactgcc tttcttttaa taatttaaac gtggtaactg gtgtgaatag
     6121 cgtgaaatag cttataaaaa aaatgattaa gtacaggcca atataaaata gcaatagtgt
     6181 tgcatcattg ttggccatta gcctagctgg ccttcctgct gtcgttagct caaggtttcg
     6241 gaagcagagt aaatacaaaa aaacagcaca gacaaatgga cttaataagc aacttctaag
     6301 tacagcattt cggtttgttt tttttattag ttttctcgtt ttaaatgtca ggataaataa
     6361 tatcaaagca agggaaggcg ctgttttaca ataaatatcc ataacctttg tcattaatgg
     6421 gttgtaggcg gaggttattg atgggatgtt ttcagtactg gtagctattg tgtgtaagaa
     6481 cagattgtct ggatttgtat agtaaacggc tagtatacag agtaaaggta taactccgat
     6541 taccataaat aaatataata agttattggc ttttctattg tctatatcta ttgaatgttc
     6601 atcaatcatc attgacctat aggattataa taaaggtata tatgaaaaaa attattattt
     6661 gtgccatttc actaggaata atgctgcttg cagcatgcca agtcaacaat gtcagagata
     6721 ctggaggtgg ggctgtttca ccatcttcca ctgttaccgg agtgagtatg ggaagtggta
     6781 gtagtggtag cctgtaaagg atcttcttga gatcctttac agatcgtcgt agtctcttgc
     6841 cctgtaaacg aaaaaaccgc ctggggaggc ggtttagtcg aaggttaagt cagttgggga
     6901 ctgcttaacc gggtagctgg cttaagtgga
//
