LOCUS       CP056905                4072 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00014 plasmid pRHBSTW-00014_4,
            complete sequence.
ACCESSION   CP056905
VERSION     CP056905.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148452
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP4 T1-00014
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87.061765885616x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 02:27:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,380
            CDSs (total)                      :: 5,260
            Genes (coding)                    :: 5,047
            CDSs (with protein)               :: 5,047
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 91 of 213
            Pseudo Genes (incomplete)         :: 118 of 213
            Pseudo Genes (internal stop)      :: 43 of 213
            Pseudo Genes (multiple problems)  :: 35 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00014"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00014_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2755..4072,1..842))
                     /locus_tag="HV035_26885"
     CDS             complement(join(2755..4072,1..842))
                     /locus_tag="HV035_26885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QMA39948.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            1021..1275
                     /locus_tag="HV035_26890"
     CDS             1021..1275
                     /locus_tag="HV035_26890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QMA39949.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            1807..2499
                     /locus_tag="HV035_26895"
     CDS             1807..2499
                     /locus_tag="HV035_26895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMA39950.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(485..843)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          844..1020
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1808..2499
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..929)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.706"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2759..4071)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.772"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttactgtcct tcggcaacgc ttcgatgatg tcacgtagtg tgtacgaagt tgcgttgtca
       61 ggaagcacga tattcagaag gtcagccaca cggcgggcag gctctgtcag cccgctgttc
      121 ctgagttcag tgcgggaaat ctgtatttcc aggttaagcc tgttaatcag acgattctgt
      181 tcgccgcgtt ctgtttcgat accgcgtgtt tccatcgcac aggcagactt tccgaggtga
      241 ataccaggcg ttttctcaag cccctgtcgc tcgtatgaac gatggtctat ctcttcctga
      301 taaccggcgt tcgccagtgc tctgtttgca tggtcagccc atgatgcacg ccacgtctcg
      361 gcatgtgtcc ggtcgttcca gtccctgacc tttccgccga acccagcagg tccgacagcc
      421 ctcgtggtca gcatgatgtg cgcatgggga tttgttttat ccatgtgatg gaacgccaca
      481 tcagcaatca tgccctgact gacaaagttt tctctgacaa aggcgagaac ggtttcccgt
      541 gcggcatcct gcggcagttc acgggggata gcgagttcaa tttcccgcgc aagttgtgag
      601 ttcttccggc attcggcctt ctcaacggca ttccagagtt cggcacggtt cgtacaccag
      661 gcaggggctg tcgcgggggt cagaatcaca gcttcagcca caccgctttt gcgggtgtag
      721 tcgtgagtca gtccggtacg ctcatcaagc atcagtgaac cggaacgata ggcagcggaa
      781 gctacggcgc tgtagccgct gcttctggag ataattttca tgctgagatg ataaatcgcc
      841 atttctgccg taatgccttt ttgtagtttt ctttcagccc cgcagggcgc acacaatcga
      901 aacggagtgg agattgtata agtgcgcact ttgtgatttg tcgcgcatta tgccagctca
      961 gtttttaaac taccagtgac gcgctaaaat gcgatttttc atcgcgcacg gagttacatc
     1021 atgagcagaa cgctcgaaca gaagattgct gatgctgagg cgagattaca gcgcctgaag
     1081 gcgaagagcc ggagcctgga cacagcgcag aaggttgttg tgggtgcagc attgctggca
     1141 aaggtcagaa agccggagga ggtgcagttg cgtgcctggt tactccagtt cctgaaggca
     1201 gaggtgacac gacaggctga tgtgacgcgc atactgccgc tgattaacga gctggaggcg
     1261 ctgccaggac agtgattgta tcgtccgctc gccgcagccc cagcgaccga gcgttagcga
     1321 gcgagtgggc gaggaagcgg aagtgaatcc gtcgccaggt tgagcacata cgcacggttt
     1381 ttcggtaggt gatatgcgct gaacccgcat gaatactgga tttccacagg gttggaaggg
     1441 gagtattcgg aacttgcaat tctgctttct tgcaacacat tgtttttatt ggttttttgt
     1501 tttcagcgcc ccaagttatc cacaggctcg ccgttaagtt tttagatctt gtttttaaga
     1561 tcttgttttt aatacttgtt ttagggtcaa aattcattaa taaaatcaat atgttataga
     1621 ggtatatgtg gaggaattta aggtaaaggt ggaggaattt aaggtgttta ggtggaggaa
     1681 tttaaggtaa aggtggagga atttaaggtg ttattcacag aaatgtgagg atataaaaaa
     1741 aaatattcca catttcttat ttgtggggca cattaaagca tcaccacatt tgaacgggat
     1801 ctttgtatgt cggacttgat tgcctacaag tcgaatgctt tggttgaggc cagctacaag
     1861 ttgaccctac aggaacaacg ttttctgttg ctgtgtatca gtcgattgaa atctggtgct
     1921 gatgctgaat tgcaaaagac aatgaccatt accgctgcgg aatattttga ttcattccct
     1981 gacatggggc ggaaaaatgc cgaggttcag ttacaggaag cgatcgatcg tttatgggac
     2041 aggtcgatca ttctcaagaa tgacgagaag cgtgaggagt tccgctggat ccagtatcgg
     2101 gcacagtacg ctaaaggtga ggccaaggct cgaataacct tttctgacgc ggtaatgcct
     2161 tatctgacgc aactgaaggg gcaattcacg cgcgttgtta taaaaaacat atcaggttta
     2221 tcaagtagtt acagtataag aatatacgag ttcctccagc aattccgcag tacgggagag
     2281 agaattattg ctcttaatga ctttcgttca atgttaggga ttgaaaataa atacaaacaa
     2341 ttcagagact taaataaaat actaataaaa ccctgtgttg acgaactgaa caaaaaaagc
     2401 gacctggctg taacggttga gacgatcaaa aaggggcgca cggttgtagc cctgcatttt
     2461 cgcttcaagg aagacaaaca gatcaagatg acgatctaaa atcgatttta aggcacctaa
     2521 aacgctccct gctgcgtttt ttttgtgtta gtagtgcgaa ggtactattg aagtgaaaaa
     2581 ttgcgtcaaa agcgctctaa tgcagcgaaa aaagaacccc caatttttgg gggcggaaaa
     2641 acaaaactat gcttgcatta tgtgtgtgct tcttgcattc atccctgtcg atgaacatag
     2701 aagaacatcg acgaacataa cacgatgtta aacaaaacaa aatattgttc acattcactg
     2761 tgaccaaccg cgatcatgtc tcctctcggt ggtgtgtgtt cttgcatctt cctcgcgccg
     2821 ccattcatcc caggtacgat tgaaacccag agagcggaac agatgatccg gctgtttttc
     2881 tggtgtgttg acggcagcaa aaagcggtgc ctcacgggtt ttcagatgaa actccataag
     2941 ttccggcgca ggtgatatgt ggctgtctgc atcgtgattt acggcaacac cataccctgc
     3001 ataccgtaac cccttatcgg tcagtgcact gatgaccagc tggctgttat cggctgaggg
     3061 aacggcaaac gcgggataca gcgtaccatt aagccggaca acaccgccca gagagtcatc
     3121 cacgcccatg accagcaaaa gctgaagctg gcgggtttcc cggtcgacag aactcattac
     3181 cggcatcagc gtcggtagca gacgtccctg cgtaaaatcc accttccttg ctgccagtga
     3241 tggcatggct ggctggcggt cttctccaag acagaaagac tccggccatg tcttcggctg
     3301 gcggagattg cgggtatatt gcggccatgt ctggcaaacc tgcgtcagaa gttgctcaaa
     3361 ggtcgcagag gaataaacac gttgcagtgg tggatgttga ccgatttcgc ttcggtcaca
     3421 gatataacca ctcctgtcac gcattaaatc aacacgctca aagattctgg tgcggtattt
     3481 ctccagctcg ttcccccaga gcgtgtattc tccgttccgg ttgtgaagca gaaggtaaca
     3541 ggtcatttcc ccgttaccag gcatgggggc ctcgccatac cccgtaacga ctgcgctcag
     3601 tacccctgaa tccataacgg cattgtcatt tttcctgagc tgttccgctc gtcgctccgc
     3661 gcgactacgt tctgtatcct cctgctcctt ccggcgcaat tcctcttcac gtttaagcaa
     3721 ctggactctg agagctgcaa ggccggatgc cccccactgc tgccactgtt tttcaaatgc
     3781 cgtctgactc gcgggaatgt cctgtttctc caccttccgg taatgctgtc gcaatttttc
     3841 catttgctgg ctcagcgact ggatttttcc ctgttcgcta cggtattccg gtgccgccca
     3901 ttgcatcagg ggaatacggg aaaaaccgga cgaaaacggc gatgattttt tcaggctggc
     3961 agcatggctc atcgcttcct ttcgttcggt gttcagctct tcccagcgtc tgcgcgtcgc
     4021 ctgcatatct gccatcatcg acatggcatt actggtgagt ttccatgccg ca
//
