LOCUS       CP056869                2181 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00087 plasmid pRHBSTW-00087_3,
            complete sequence.
ACCESSION   CP056869
VERSION     CP056869.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148479
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP5 T1-00087
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 116.204574884614x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 01:43:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,030
            CDSs (total)                      :: 4,912
            Genes (coding)                    :: 4,757
            CDSs (with protein)               :: 4,757
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 76 of 155
            Pseudo Genes (incomplete)         :: 72 of 155
            Pseudo Genes (internal stop)      :: 42 of 155
            Pseudo Genes (multiple problems)  :: 30 of 155
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00087"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00087_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            201..626
                     /locus_tag="HV062_25145"
     CDS             201..626
                     /locus_tag="HV062_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QLZ90732.1"
                     /translation="MVKNKRSGSETRQRSKVITLRINNKIDREIRRLAKAEGMTISEYI
                     RTSCLNKEIIQRIPSRYLDELLELGRMQKNLFDKGRTPKDKDYLDVMHKIITLCAEAKE
                     VILRNSDIYKEMDLLKQEIKIIKQYYKNKYPGSDLFN"
                     /name="mobilization protein"
     gene            complement(668..1195)
                     /locus_tag="HV062_25150"
     CDS             complement(668..1195)
                     /locus_tag="HV062_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="QLZ90731.1"
                     /translation="MSVERYKSNPFLENMIVPVKGRQVKLSRLGKDNNILMNQSTGEFL
                     GTHVTTYKQVDADQFIKIFTDNIAMTFDFTSAGIKAFGVLLWAVQHHALSKDEVDLDSF
                     ILDAFISENNKENKKIRLSMATFKRGINELEKASIIAKTLRKGRYYINPNFVFNGDRIA
                     FTNLIERKAKDK"
                     /name="replication/maintenance protein RepL"
ORIGIN
        1 aaatattgcg tgatgccttg cttttttgta ttgaagatac cagcttatga ggtagttttt
       61 tattttccgc cgaaaagttg tagcgaaaaa cgggacagga tgtgtatttg tcataccgcg
      121 aatacattcg cttgtatgaa aatacctcct gtcagggcag gccccgacac cccgaacgtg
      181 gaaaccagag gttcattgtg atggttaaaa ataagcgcag cggctcagaa acacggcaga
      241 gaagcaaggt tataaccttg cggataaata acaagattga cagggagatc cgacgcctgg
      301 caaaggcgga agggatgacg atttcagaat atataagaac gtcatgttta aataaagaaa
      361 taatacagcg catcccttcc agatatttgg atgaattatt agagcttggc cggatgcaga
      421 agaatttatt tgataaagga agaacaccga aggataagga ctaccttgat gtgatgcaca
      481 aaataatcac gctgtgcgca gaagcgaagg aggttatttt acgtaattct gatatatata
      541 aagagatgga tttattaaaa caggaaataa aaataattaa acagtactac aaaaataaat
      601 acccaggctc tgatttattt aattaaaata aaccctgctc aatttttaga gcggggttta
      661 tatgttgtta tttgtctttt gcttttcttt ctatcagatt tgtgaaggct attctatcac
      721 cattaaaaac aaagtttggg tttatataat aacgtccttt tcttagggtt tttgctatta
      781 tacttgcttt ctcaagttca ttaatgcccc ttttaaatgt ggccatactc aaccttatct
      841 tcttgttttc tttattattt tcagagatga aggcatccaa tataaaacta tctaaatcaa
      901 cttcatcttt agataatgca tgatgctgaa ccgcccaaag aaggacacca aaagctttaa
      961 ttccggcgga agtaaaatca aatgtcatcg ctatattatc tgtaaatatt ttgataaatt
     1021 gatcggcgtc gacttgttta tatgttgtca cgtgagtacc aaggaactct cccgtcgatt
     1081 gattcattaa tatattatta tctttgccta aacgagataa tttaacctgc ctacctttga
     1141 caggcacgat catattttcc aaaaatgggt tcgatttgta cctttcaacg ctcataatgc
     1201 accattatat tgaaccttag tagctaaaaa agcgcccttt caaagctcat aatgaacgaa
     1261 ctatttgagc tttgaaagct cacactatag ctaacacggc tcaaaaacaa cacctatttt
     1321 gagccgtgtt ggtaacaggg ttgagctaac aagttacaga tccgaagatc acgatcccca
     1381 tttcatgcgg gttgcagagg attcccttct tagttcttat acggtggtgc gtaagtgccc
     1441 taggctgaca tctggttcac ttccgcttcc tcgcccactc gctcactaac gttcggtcgc
     1501 tggggctgcg gcgagcggat cctcctctct cgtagtggct ggcttttggt gttgttcttt
     1561 cgaagatgag tcgtcagcat ggccctaaaa cgcgctcaga gcctctcaaa cgcacgaaac
     1621 ggggtttaaa tgcccgtgag ggcatggaag gcgcgaaaaa cgcttacaga gggtttcagg
     1681 aggccaggcg cgccgatacc cctaactagg ggctttcagg tcgctaggaa ccgcgcaagc
     1741 cccctgcaaa gcccaaccgc acgggacagg ccgccactcg gttggtgggt ggtcgaagac
     1801 cgttggtgtt acagcggcgg gggtctgggg gtctccccag acggtttccg gtagcgctca
     1861 aggcgctgtc ggaaccgtgg cgggtttttt ttaacaaaaa acgacaggtc ggaaattaaa
     1921 gcgcgaactg aacttatatt tcacatatat aaacgcgata gttatcatgc acacacatat
     1981 atattcgctt catcgttgta atctgtgcga tttctgcctt aatcgagcat attgacggca
     2041 tcggttaaca ctgaaaataa tactatcgcg aggattaaat aattatttag catatatata
     2101 ccgcagacga taagagaaga aacgtaatat caaataaaaa taataaaaaa ataacaagca
     2161 ccattttaaa acctgtgtaa a
//
