LOCUS       CP056806                1976 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00176 plasmid pRHBSTW-00176_7,
            complete sequence.
ACCESSION   CP056806
VERSION     CP056806.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148525
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00176
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 49.6754890387248x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 02:24:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,408
            CDSs (total)                      :: 5,280
            Genes (coding)                    :: 5,034
            CDSs (with protein)               :: 5,034
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 99 of 246
            Pseudo Genes (incomplete)         :: 137 of 246
            Pseudo Genes (internal stop)      :: 52 of 246
            Pseudo Genes (multiple problems)  :: 39 of 246
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00176"
                     /isolation_source="Freshwater sample from upstream of
                     wastewater treatment plant"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00176_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(995..1976,1..8)
                     /locus_tag="HV108_27030"
     CDS             join(995..1976,1..8)
                     /locus_tag="HV108_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QLZ05232.1"
                     /translation="MKNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
                     /name="protein rep"
     Typing          complement(7..88)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.181"
                     /coverage="83.0"
                     /evalue="8.21e-29"
     Typing          complement(334..428)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.82e-43"
     Typing          complement(1711..1902)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.482"
                     /coverage="100.0"
                     /evalue="1.32e-94"
ORIGIN
        1 caggctagcg gggtgtcggg gcgaagccct gaccaggtgg ggaatgtctg agcgagcgtg
       61 cgcggtccga cattcccaca tcctgtcccg aaaattcccg ccgtttctcg gttttttgag
      121 cgtcgcagac cgcacgacaa acgaaacgaa gtgtagtttg tatgagtgcg cacttcgtga
      181 gattccccga ctgccagttt gtcgatcgcg cggaacagct gcggcacgaa aacggcagaa
      241 tgatttgctg tccggttgcc agaactgtgc gcttacgtgg agggcggcgc ggcgcaaaaa
      301 ctgccgacat tgcgccagtg ttccggtgac acaaacccgc agaaatcggg acaggatgtg
      361 taatcgtata accgcgcacg cacagccatc cgattaccac ctggtcaggg cttcgccccg
      421 acaccccgcc aacgcttcac ggcacgcgaa aaaaaattcg gcgtcccgct cactgttccc
      481 gaactcgtca aacagaaaat cgcgggggcc cctccggggt tccgattttg tgattgacga
      541 cggcacggaa aaaaacactg gggatggtgg ttggtgtcac gcagtgaggc caaccacctg
      601 caagacggtt tttgtttttc acaaaatcgc tggaaaaacc tgtgagtgtt ttgcggggaa
      661 aacgcttttg tggttgttgt ttttgctgtc cgaaaatcag ctcagatttt gagcaaatct
      721 cgcgcgcgtt ccttgtattt atacttaagg gataactcta ggattcaaat tggcctccag
      781 cccttgtccc gcaaggcctg aggggggatt tgtcggaaaa tggttttggc gttattctgt
      841 cgggagtggt tgcgttaaaa gtgacccccg caacgacggg catcgttcgg gggtgtgacc
      901 acctgtagaa attcgaatta gtgacacgac aacacaggtg atatggcaat gataacacgt
      961 cagatgagcc atcgcccaca atcgggtggt gcgcatgaaa aaccctaaac tccagtacct
     1021 cactgattat tccccctctg atgcgccctg ggacgcacac agaagcgttt ctgacgacgt
     1081 gggagggatt tacctgcttg cggccgaata tgagcgctac ggggctcgta tggcctcctg
     1141 tggcggttta ctgcgtttcg ggtggtctac cctcaaggaa accggagaaa cgcgtctgag
     1201 gctgcgtgag gcacatttct gccgtgtccg gcactgtcct gtctgccagt ggaggcgctc
     1261 cctgatgtgg caagcccgtt tctaccagtc cctgccgcgt atcgtggcgg attatcccga
     1321 tgcccgctgg atgttcctga ccctgaccgt gcgcaactgt gcgattgggg aactgggcga
     1381 gatgctgaac cggatgaatg cagcttttca gcgtatgaag gttcgcaagg agttcctgcc
     1441 tgttcagggc tggatccgca caacggaagt tacccgcggc agcgacggca gcgcacaccc
     1501 gcacttccat accttgatga tggctcctcc tggcatgcta aatgggaaaa gttacgtcag
     1561 gcatgaacgc tgggttgagc tatggcggga gtgcctgcgg gttgattatg acccgaatgt
     1621 tgatgtccgg gcagtgaaac cccgcaagcc gaaggatggc gagtccttag cctgcgcgac
     1681 cgctgaactg gtgcgcggcg cagtggccga aaccctgaaa tattccacga agccggctga
     1741 catggtggcc gatcctgaat ggttcctcga actgacccgc cagacgcaca agcgccgctt
     1801 tgtggcgacc ggcggcgcac tcaaagacgt gttgaaactc gatcaggaaa ccgacgccga
     1861 catggtgatc ggggatgata tttccgaggg cgacgatgac ggttcacgaa tcgcgttcga
     1921 gtggaaaacc gaggtcagaa agtaccgtcg ctcaccgacg aaagataaaa ctgaat
//
