LOCUS       CP056792                1976 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00180 plasmid pRHBSTW-00180_7,
            complete sequence.
ACCESSION   CP056792
VERSION     CP056792.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148528
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00180
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77.3547982687507x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 00:15:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,955
            CDSs (total)                      :: 4,830
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 97 of 227
            Pseudo Genes (incomplete)         :: 119 of 227
            Pseudo Genes (internal stop)      :: 47 of 227
            Pseudo Genes (multiple problems)  :: 31 of 227
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00180"
                     /isolation_source="Freshwater sample from upstream of
                     wastewater treatment plant"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00180_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(571..1560)
                     /locus_tag="HV111_24770"
     CDS             complement(571..1560)
                     /locus_tag="HV111_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QLY95120.1"
                     /translation="MKNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
                     /name="protein rep"
     Typing          152..246
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.82e-43"
     Typing          492..570
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.25"
                     /coverage="80.0"
                     /evalue="8.21e-29"
     Typing          654..845
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.482"
                     /coverage="100.0"
                     /evalue="1.32e-94"
ORIGIN
        1 gacaccaacc accatcccca gtgttttttt ccgtgccgtc gtcaatcaca aaatcggaac
       61 cccggagggg cccccgcgat tttctgtttg acgagttcgg gaacagtgag cgggacgccg
      121 aatttttttt cgcgtgccgt gaagcgttgg cggggtgtcg gggcgaagcc ctgaccaggt
      181 ggtaatcgga tggctgtgcg tgcgcggtta tacgattaca catcctgtcc cgatttctgc
      241 gggtttgtgt caccggaaca ctggcgcaat gtcggcagtt tttgcgccgc gccgccctcc
      301 acgtaagcgc acagttctgg caaccggaca gcaaatcatt ctgccgtttt cgtgccgcag
      361 ctgttccgcg cgatcgacaa actggcagtc ggggaatctc acgaagtgcg cactcataca
      421 aactacactt cgtttcgttt gtcgtgcggt ctgcgacgct caaaaaaccg agaaacggcg
      481 ggaattttcg ggacaggatg tgggaatgtc ggaccgcgca cgctcgctca gacattcccc
      541 acctggtcag ggcttcgccc cgacaccccg ctagcctgat tcagttttat ctttcgtcgg
      601 tgagcgacgg tactttctga cctcggtttt ccactcgaac gcgattcgtg aaccgtcatc
      661 gtcgccctcg gaaatatcat ccccgatcac catgtcggcg tcggtttcct gatcgagttt
      721 caacacgtct ttgagtgcgc cgccggtcgc cacaaagcgg cgcttgtgcg tctggcgggt
      781 cagttcgagg aaccattcag gatcggccac catgtcagcc ggcttcgtgg aatatttcag
      841 ggtttcggcc actgcgccgc gcaccagttc agcggtcgcg caggctaagg actcgccatc
      901 cttcggcttg cggggtttca ctgcccggac atcaacattc gggtcataat caacccgcag
      961 gcactcccgc catagctcaa cccagcgttc atgcctgacg taacttttcc catttagcat
     1021 gccaggagga gccatcatca aggtatggaa gtgcgggtgt gcgctgccgt cgctgccgcg
     1081 ggtaacttcc gttgtgcgga tccagccctg aacaggcagg aactccttgc gaaccttcat
     1141 acgctgaaaa gctgcattca tccggttcag catctcgccc agttccccaa tcgcacagtt
     1201 gcgcacggtc agggtcagga acatccagcg ggcatcggga taatccgcca cgatacgcgg
     1261 cagggactgg tagaaacggg cttgccacat cagggagcgc ctccactggc agacaggaca
     1321 gtgccggaca cggcagaaat gtgcctcacg cagcctcaga cgcgtttctc cggtttcctt
     1381 gagggtagac cacccgaaac gcagtaaacc gccacaggag gccatacgag ccccgtagcg
     1441 ctcatattcg gccgcaagca ggtaaatccc tcccacgtcg tcagaaacgc ttctgtgtgc
     1501 gtcccagggc gcatcagagg gggaataatc agtgaggtac tggagtttag ggtttttcat
     1561 gcgcaccacc cgattgtggg cgatggctca tctgacgtgt tatcattgcc atatcacctg
     1621 tgttgtcgtg tcactaattc gaatttctac aggtggtcac acccccgaac gatgcccgtc
     1681 gttgcggggg tcacttttaa cgcaaccact cccgacagaa taacgccaaa accattttcc
     1741 gacaaatccc ccctcaggcc ttgcgggaca agggctagag gccaatttga atcctagagt
     1801 tatcccttaa gtataaatac aaggaacgcg cgcgagattt gctcaaaatc tgagctgatt
     1861 ttcggacagc aaaaacaaca accacaaaag cgttttcccc gcaaaacact cacaggtttt
     1921 tccagcgatt ttgtgaaaaa caaaaaccgt cttgcaggtg gttggcctca ctgcgt
//
