LOCUS       CP056791                2088 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00180 plasmid pRHBSTW-00180_6,
            complete sequence.
ACCESSION   CP056791
VERSION     CP056791.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148528
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP1 T1-00180
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77.3547982687507x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 00:15:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,955
            CDSs (total)                      :: 4,830
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 97 of 227
            Pseudo Genes (incomplete)         :: 119 of 227
            Pseudo Genes (internal stop)      :: 47 of 227
            Pseudo Genes (multiple problems)  :: 31 of 227
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00180"
                     /isolation_source="Freshwater sample from upstream of
                     wastewater treatment plant"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00180_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(1911..2088,1..89)
                     /locus_tag="HV111_24760"
     CDS             join(1911..2088,1..89)
                     /locus_tag="HV111_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLY95119.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            942..1907
                     /locus_tag="HV111_24765"
     CDS             942..1907
                     /locus_tag="HV111_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QLY95118.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          1336..1567
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ttcagaaaac aaacgagagg gtaaaaactc tttttgtgaa aatagattta aacggtatca
       61 agtttggatt tttaagaacg cattcttagt tctggaaaag agccagcggc aggctgaggt
      121 gataggtacg agattgcatg caatctctag tgctctgtct atcctgcatt atcctcagca
      181 ttatcctcag ccttgccaac tcgacaccaa tgcaggatag acaatccgat gtcaaatgtt
      241 aacactctgc gagtggtaca ttttccccgg attatcgtcc tgagcctgcc gctggctctc
      301 tttctaccgc ctcgctttgc tcgttgctca acgcctcaca gacacggatt aaaatccgca
      361 tccgttcacc gttttttaaa gtccgttaaa agcatgatgc catctccgag agttaatctc
      421 gtcaaatgct aaatcgtggg ggtccccttt ggggttccga tttagtgatt gacgacacca
      481 ccgattaaaa aacttatgcg gggtggatgg tttcacgaag tgaggccatc cacctgtaag
      541 acagggtttt gtttttattc cctgttttgg tgatcgggtg tgtggaaaag gttggggtaa
      601 gccgttcggg ggtgcttgtt ttgggggtta aaattgtggt tattttttgc gcaattctcg
      661 cgcgtgatcc ttgtatttat acttaaggga taaatggcgg atatgaaata gtggtttagc
      721 ccagtaatga cgaggctttg agtgggtttt gacaggtcaa agaaaatgga gcagaattga
      781 ggcgttttta atcggcgttg gggagtgcgt caacactccc caacatttcg aatgtgtcac
      841 ctcagcggca aactctggtg acatgtactg gctcgcaatg cacaggtacg tgatgaatat
      901 accacatcaa atcacagcct gcccagatcg gagcaggctt aatgtcagaa gataaattcc
      961 tttcggacta cagcccccgt gatgcagttt gggataccca gcgcacgctt accgattctg
     1021 tcgggggtat ctaccagact gctgctgaat tcgagcgcta tgcactccgt atggcctcct
     1081 gtagcggttt gttacgtttt ggttggtcta ccatcatgga aaccggagaa acgcgcctac
     1141 ggcttcgtag tgcgcaattt tgccgtgtcc gtcattgccc tgtctgccag tggagaagaa
     1201 ccctcatgtg gcaagcccgt ttttatcagg ctctaccgaa aatcgttgtg gattacccgt
     1261 cttcccgatg gttgtttctg acgttaactg tcaggaactg cgagataggt gaacttggaa
     1321 cagtccttac agcaatgaat gcggcgttta agcgaatgga aaagcgaaag gagctatcac
     1381 ctgttcaggg gtggatcagg gctacggagg tgacgcgagg taaggatggc agcgcacatc
     1441 cgcattttca ctgtctgctg atggtgcaac cttcttggtt taaagggaag aactacgtta
     1501 agcacgaacg ttgggtagaa ctctggcgcg attgcttgcg ggtgaactat gagccgaata
     1561 tcgatattcg ggcagtaaaa actaagacag gtgaggttgt ggccaacgtt gccgagcaac
     1621 tgcaaagcgc ggttgctgaa acgctgaaat actccgttaa accggaagat atggcaaacg
     1681 atcctgagtg gtttcttgag ctgacgcggc agcttcacaa gcgccgtttt atctcgaccg
     1741 gtggggcgct aaaaaacgtc ctccagttgg atcgagaaac caatgaggat cttgtcattg
     1801 ccgacgatgt aggggatggc actgatgacg ggaagcggac ggcgtttgtc tgggattcag
     1861 gtaaacggcg ttacaaacgc gcccctgaga aggataaatc ggattaacgt atgaatatta
     1921 atattgaata cctgaatgga aataagacta ttggtttatt ttttttaaga agtgaagcgg
     1981 tgattcctga caggtttaaa aaccttattt tgcttattga tggattaagt tttggcacat
     2041 ttggttttca tccgcacgaa ggttttgagg atgaattaat tttatata
//
