LOCUS       CP056770                2495 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00675 plasmid pRHBSTW-00675_5,
            complete sequence.
ACCESSION   CP056770
VERSION     CP056770.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148680
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00675
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 113.634895689311x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/22/2020 15:17:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,811
            CDSs (total)                      :: 4,694
            Genes (coding)                    :: 4,601
            CDSs (with protein)               :: 4,601
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 27 of 93
            Pseudo Genes (incomplete)         :: 60 of 93
            Pseudo Genes (internal stop)      :: 17 of 93
            Pseudo Genes (multiple problems)  :: 10 of 93
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00675"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00675_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(2437..2495,1..169)
                     /locus_tag="HV263_24045"
     CDS             join(2437..2495,1..169)
                     /locus_tag="HV263_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="QLV85460.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            535..720
                     /locus_tag="HV263_24050"
     CDS             535..720
                     /locus_tag="HV263_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLV85461.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1011..1202)
                     /locus_tag="HV263_24055"
     CDS             complement(1011..1202)
                     /locus_tag="HV263_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLV85462.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(768..855)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1494..2022
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ctgcacgcgc acaagctatg ccatacacac caatgacggt cgcactattg tcagcgacgg
       61 caaacctaaa gaatcagact caggcctgct tggctataca gacgcgaatg gtgtcaaaca
      121 gcagatcaat aaaaccgacg ttaaagaagt ctccgaaatt ccgcactgag catcttctcc
      181 gaaaaaggcc gctaatgtgg cctttttctt tacctgtggt tctccgccaa aatcccagca
      241 aattgcatca ccaaagctaa aagctttcag ggttgtctaa aaagcacaag acataagagg
      301 aagtgcggta ttttataatc aagcccccaa gaatttacca taacatccgt tgcccgcacc
      361 gcctgagacg cgttcagcgc gttcctgacg aaaccatgac aaaaaccaca acaaaccacc
      421 ccggaacccg tcagaaacgc gcctgttaaa ttttaacggc atgcatgact atgcaccaga
      481 atgacgccat gctcttttca cgcaaaaatc atcaccagac ggggaaaatc accagtgacc
      541 agacaggaat ccgccgccct caatatggcc aaatttatcc gcgcacagac acttctcctc
      601 cttgagcggc tcgagcagat ggatctggat gaggctgccg gctgctgtga gcacctgcac
      661 gatcaggccg aagcgcttta cgccatgctg aacgcacaga taggcgagga aaatgcgtga
      721 aaatcggtga acgggtgcgc aattcagtgc gcggccgtga ggcgatggcg gggtgtcggg
      781 gcgcagccct gaccagggta tttgtgatgc cggcgcgtgc gcggtattac aaatgcacat
      841 cctgtcccgg aacggacacc gggaaacagc aaaaaaaacc gggcggcacg cccggaactc
      901 aatcaagtta gattagatta ctctcactcg tccataacag catcatggaa cgacgaccac
      961 cgtccgtgac ggccgcctcg tttaagtatg gacagaaata cagaaaatgc tcaggacgaa
     1021 atgtaatgaa tgcgaacgga ttcaagaaat tcgagcatga cagtccttac ggccggttcg
     1081 gtttcagaca aaatctgccg gtatgcatcc agcatcatgg ctccggcatc ccctccggca
     1141 cgccgtagcc agaccgaaac aacggacaca agcaggtgtc gctcatcatc actaagagtc
     1201 atcagggctc cggaagaaaa accaaacatg gcttacctac tgttaacggc aacgcagaag
     1261 aaaggtttac agggtagcgg gcagggatga aaaaaaaaaa attgcagtca ggccggaacg
     1321 gctgctgaac cggtacaggc gggttccggg cgcagcgccg gaccagtcac ggagcactag
     1381 cggagtgtat actggcttaa gatgtagcat ggcagtaaca catagcatta atgccacata
     1441 agacaggcgc agaaagtgaa agaaaacgcg tcaggagaaa atgtgataca gaatatattc
     1501 cgcttcctcg ctcactgact cgctccgctc ggtcgttcgg ctgcggcgag cgggacgggc
     1561 ttacggatgg atcagaaatt tcctggggga tgccaggaag atacttaacg aagaaagcat
     1621 aaggccgcgc caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacagaatc
     1681 tgacgctcaa atcagtggtg gcgaaacccg atgcggctta aagataccag gcgtttcccc
     1741 ctggtggctc cctcgtgctc ccccgcattc ctgcctttcg gtttaccggt gtcactccgc
     1801 tgttatggcc gcggtttctc attccacgcc tgacactcag ttccgggaag gcagttcgct
     1861 ccaggatgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     1921 actatcaact tgagtccaac ccggaaagac acgactaatc gccattggca acggccattg
     1981 gcaaacagga tgtgcgagag atgtctcaag agttcttgaa gtggcggcct tagtgaggct
     2041 acactgaaag gacaaatttg gtgacatgtc tcgcacaaga ctgttaccac ggttagggga
     2101 tctgccagga ttacctaacc ttcgaaaaac cacctgccag ggtggttttt tcgtttacag
     2161 ggcaagagat tacgcgcgga aaaaaaggat ctcaagaaga tcctttataa attagacatc
     2221 caacctcaca aagaggaata cttaaatcat cttggtatat gtatcggccg gattacatct
     2281 catataagcc tcctccccgc ctgacaccac actttgacca tagcacctcg cgcctaactc
     2341 gatatacagc ccaccatcaa gcctcttttc acataaaaaa tgacctatag ttagatggtt
     2401 agtgaccaaa actgatatgg ataaaaggag ctggctttga aaaagatttt tgtttcagtg
     2461 ttcgcagcgg cagttgcgct ttcagccctc accgg
//
