LOCUS       CP056769                4995 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00675 plasmid pRHBSTW-00675_4,
            complete sequence.
ACCESSION   CP056769
VERSION     CP056769.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148680
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00675
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 113.634895689311x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/22/2020 15:17:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,811
            CDSs (total)                      :: 4,694
            Genes (coding)                    :: 4,601
            CDSs (with protein)               :: 4,601
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 27 of 93
            Pseudo Genes (incomplete)         :: 60 of 93
            Pseudo Genes (internal stop)      :: 17 of 93
            Pseudo Genes (multiple problems)  :: 10 of 93
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00675"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00675_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(115..330)
                     /gene="mbeD"
                     /locus_tag="HV263_24005"
     CDS             complement(115..330)
                     /gene="mbeD"
                     /locus_tag="HV263_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QLV85453.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="mbeD"
     gene            complement(337..828)
                     /locus_tag="HV263_24010"
     CDS             complement(337..828)
                     /locus_tag="HV263_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QLV85454.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(803..1348)
                     /locus_tag="HV263_24015"
                     /pseudo=""
     CDS             complement(803..1348)
                     /locus_tag="HV263_24015"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1506..1829)
                     /locus_tag="HV263_24020"
     CDS             complement(1506..1829)
                     /locus_tag="HV263_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QLV85455.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2959..3267
                     /locus_tag="HV263_24025"
     CDS             2959..3267
                     /locus_tag="HV263_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="QLV85456.1"
                     /translation="MKNSFELISTDPVKFNTWSIKSKLIIIIRKLMIDNKWSQSEAAEK
                     LGISQPRVSNMLNGKLDKFSVDTLLEMVFKMGYKLDMDFTPLNTESPLTMVVKKAMV"
                     /name="XRE family transcriptional regulator"
     gene            complement(3283..3615)
                     /locus_tag="HV263_24030"
     CDS             complement(3283..3615)
                     /locus_tag="HV263_24030"
                     /inference="COORDINATES: protein motif:HMM:NF017757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QLV85457.1"
                     /translation="MSNDENEYTFKPVNAQAIKALKSLPEWVQAAFVHALDSVFYGVDP
                     GMSIAHLTVIGPGVVELRINGRPAYRCVYRSKAPGQVDLLHAFVKTCQGPDKKNMATTA
                     SRYKLL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4057..4260
                     /locus_tag="HV263_24035"
     CDS             4057..4260
                     /locus_tag="HV263_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017191052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLV85458.1"
                     /translation="MAIYVGPHSKVGRNGGSFQECDINTTNHTVTRKYGRYCTIRDNEY
                     APPTSVPGRHWLQIFRTPDNTE"
                     /name="hypothetical protein"
     gene            4396..4623
                     /locus_tag="HV263_24040"
     CDS             4396..4623
                     /locus_tag="HV263_24040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLV85459.1"
                     /translation="MGYHYKELTVIELTAEEFELHKEGYSLELLTNMRNEEHTKYWEVC
                     PSCEGKMDPEENQGHEGYCATCWLDNPDWW"
                     /name="hypothetical protein"
     Typing          complement(1943..2116)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          2083..2609
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(24..1516)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.6"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtcagctgc ctggatggtt taaagtgtcg gcccgcggta ggttttctgc ggctggcgct
       61 ccagctccag tgttttctga tactcattct ccctctgccg gaactcggtg gcattcagtt
      121 cccggacaag cggcgcagct gctcgcttaa actcagcacc tgcgtgctca agtccgacac
      181 gcgctcgttc agcaccgcgt tctcccgttg cataagccca cacatgctcc gccattccgc
      241 gaaggcgctc tcccattcgt ccagcctttg cgagtagtcc tgctgtagct gctctaatgc
      301 gctcagcaac tgcttttcca gctctgtcat tttccgctac tcctgtttca ggcggatcca
      361 tgtcgtcccg tcgtagggga tggcttcggt ttgcatcccc ggcggcagga taagaaacct
      421 ccggccgtcg ctgctctcct gatagcgcac tccccacgtc cgggcggtca ttttcgccag
      481 ggtatcttcc tgctgacgaa gatgcgtgta attgtcggtt atctgctgcc cctgccacca
      541 cagaatgctc ccgctcgtgc cgatcagcag cgctgacacc atcaggatgg tgagccatgt
      601 ccgcccggca gtccgcagta cgctccgccg gtttttctcc atcgcctcgc tcatactctg
      661 ctcgtgggcg aggatggcgt cgctgattct cctcgcgctt gcgctcagtt ccgctctgac
      721 agactgctcg tgctcgctga atgcggcttt cagcatctcg ccggtactct gctgctgcgc
      781 tttcgattgc tgctctaagt ccttcgccag cgttaaaaga ctgctcatag atagctccct
      841 tcagtcggat gtttcgcccc ccgtccgggt cggcaatgct gatactgctt ttcgtggtgc
      901 gcaccacctc aaaacctgca ccttcaagag tgtcagtgac atcctgacgg cttttcagct
      961 ccccggacga agcaagggtc agcagtcccc gggtaatggc ttctgcggcc tcctgcttcg
     1021 ttttcggcag cccggacggc gtgaccagcg cccggcggtt ttccggcgcg ttcgggtcgt
     1081 gcagccccag tcggccgttc acgatagtct gccaggcatc gatgcgcggc cggtcggcgc
     1141 ggtcgtagta aggctggagc cgcctgccgg tcagcagctc ggtattgggg atcagaaagt
     1201 tcagctccag acgccccttg tccgcgtgct ccacccacag aatgctgtac tgatctttat
     1261 cgagtccggg catcagaacc cgctcgaagc tggctatcag tttttcccgc tggccgggag
     1321 gcagatcggc ctccgcaaac gacagaacgc cggaggtgta tttcttaacg taaggcgacg
     1381 cgtcaatcag ctcccggact tcttccggct tcccctgcag gacggtggcg ccttcacgct
     1441 gccgatcttt acccagcaga tagtccaccg gcccggcgcc accgccgcgg ccgcgcggat
     1501 gaaatttaac gatcatcgtc cgttcctttc tcaagaacgg cgtgccgcag ccgctcaagg
     1561 ccggcatcga tggccatcag cgccgccaca atctgtaccc tgtcatggcc ggtgccgccg
     1621 ccggcgttaa cacggcgggc tatctggttc aggttattgc ccatgccggc aagctggcgc
     1681 agcagcgccg gcgagatcga ggggagcttt ccggaacgtg ccggcttttc gtccaggcag
     1741 atctgccgca tccacgccgc cagctgcctg ccgtcacagc gctcgagcag ccgccggtgc
     1801 tcgtcctccg tcacccacat ggtgagcatc ttgctccgtt tctctgacaa accggcctcc
     1861 tgataacgcc gggacgggcg ggaaagcctt tcccggtcgg cgcggggttg gacaagccgc
     1921 aggcgcgtca ggcttttggc gggtttcggg gcgcagccct gaaccagtca cgtagcgcta
     1981 gcggagtgta tactggctta ctatgttggc acgaatgcgg atgtcagtga agtgcttcat
     2041 gtggcaggag aaaaaatacg gcaccggcgc gtcagcagaa tatgcgatac aggatatatt
     2101 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggaaatgg
     2161 cttactggcg gggcggaaat gtcctggaag atgccaggaa gaaacttagc agggaagcaa
     2221 taaggccgcg gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcacaaaat
     2281 ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2341 ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcactcc
     2401 gctgttatgg ccgcgtttat ctcattccac gcctgacact cggttccggg aaggcagttc
     2461 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gcctgttccg
     2521 gtaactatcg acttgagtcc aacccggaaa agcacgactt aacgccactg gcggcagcca
     2581 ctggtaaagg gtgtacaaga gataactgat gcagagttct tgaagtatca gtccaactac
     2641 ggctaaactg gaaggacgaa ttaggtggca gcggtcttgt acaccctgtt accacggttc
     2701 ataaacttgc cggtttaatg aaccttcgaa aaaccacctt gccgggtggt tttttcgttt
     2761 atgggcaaag actactcttt gctggattga tacgtcaaca caataagctt tacttaaaag
     2821 atcaaaatgt ctttacactt tttcctcttg aaagcaatat cattacttgt ataatgcccc
     2881 gcccgtatat atatataagg agttccttgc cggaactttt atcattgcga tacaggtaat
     2941 ccgaagtgga gctgttctat gaaaaattca tttgagctga tcagtacaga cccagtaaag
     3001 ttcaatacat ggtcgatcaa gtcaaaactc attatcataa ttagaaaact aatgattgat
     3061 aacaaatggt cgcaatcaga agcggcagaa aagttaggta tcagtcaacc aagagtaagc
     3121 aatatgctga acggaaaact ggacaaattc tccgtcgata cgctgttgga aatggttttc
     3181 aagatggggt acaagctgga tatggacttc actccattaa acacagagtc gcccctgact
     3241 atggttgtaa aaaaggcgat ggtgtaacca tcgccaattt gattagagca acttgtagcg
     3301 cgaagctgtg gttgccatgt ttttcttatc tggtccttgg cacgttttca caaacgcatg
     3361 cagtaagtcc acctggccgg gtgccttact gcggtataca cagcgataag ctgggcgacc
     3421 attgattctc aactcaacaa cgccggggcc gatgaccgtc aagtgtgcga tggacatccc
     3481 cggatccacc ccgtagaaca ccgaatccaa tgcgtgcacg aatgcagcct gaacccattc
     3541 tgggagtgat tttaatgcct tgatagcctg ggcattaaca ggtttgaacg tgtattcatt
     3601 ttcgtcgttt gacataaaca tcctcatcta ggttattgat ttttagcaac ttaactattg
     3661 ttaaaacgtt tcgcatataa actccgattg attgattgat ttatgagcat aaaaaaacca
     3721 caccgcccta tatttaggaa agtgcgcgca tggttattgc tcgttgaacc aacaattcat
     3781 tggcaatctc gccaacgatg ccatagtata taaattctta tatgtcatca attcttatta
     3841 gtgcttcaaa cgtctgtttg aataaaaaaa aatcaatttg acttaggcgc agaagaccta
     3901 acagttacat gtgttggttt tccttacatc aagatatgag gattttatcg acgcaatgtg
     3961 gaaagccaac aaagcacact atgatattga taaatataga aattaataaa atctacttag
     4021 atactggtaa tcaactatct gacgaaggag cttagaatgg ctatttatgt tggtcctcac
     4081 agcaaagtag gtcgtaatgg tggttcattc caggaatgtg atatcaacac aacgaatcat
     4141 acggtaaccc gaaaatatgg ccgctactgc acaatccgcg ataatgagta cgcccctcca
     4201 accagcgttc caggccgcca ttggctgcaa attttccgca cgccagataa cacagagtaa
     4261 ttcatcttct gcttagtaaa gcggtaatat cccttgtata aaccggatag gattatcatt
     4321 gtaagatact gaaaaccctg acatttaatg cttttcagta tcttagtatt gagctgcata
     4381 aagcgaggca agcgaatggg ttaccattac aaggaattaa ccgtcatcga attaactgcg
     4441 gaagagtttg agcttcataa agagggatac tctttagagc ttttaactaa catgagaaat
     4501 gaagaacaca ccaagtattg ggaagtttgc ccttcctgcg aaggaaaaat ggatccagaa
     4561 gaaaatcaag ggcatgaagg ttactgtgcc acatgctggc tcgataaccc tgactggtgg
     4621 tagttaataa tttaccataa catccgttgc ccgcaccgct gaaacggggt tggctgcgtt
     4681 tccggtgcca gacgcgagaa agtggcgaca gacataacga aaaaacacca gaaacgcagc
     4741 cgctgaatat tacactggtg catagtcatg cagccagatg catggatgtt aaagtcacac
     4801 taaaatccgc gccaatgcct taaaaaccga caaatgcttc tccagccagt cccacagctc
     4861 cgccgccaga tacaggcagt taagcaatga ttcaataaga tttaacagct tcatttcccg
     4921 ctcctgaata agtcgtgacc tttttgatca cgaacctatt tagcgggaga aataagagcc
     4981 cctcttcgtg cgggt
//
