LOCUS       CP056768                5724 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Enterobacter cloacae strain RHBSTW-00675 plasmid pRHBSTW-00675_3,
            complete sequence.
ACCESSION   CP056768
VERSION     CP056768.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148680
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T2-00675
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 113.634895689311x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/22/2020 15:17:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,811
            CDSs (total)                      :: 4,694
            Genes (coding)                    :: 4,601
            CDSs (with protein)               :: 4,601
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 27 of 93
            Pseudo Genes (incomplete)         :: 60 of 93
            Pseudo Genes (internal stop)      :: 17 of 93
            Pseudo Genes (multiple problems)  :: 10 of 93
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00675"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:550"
                     /plasmid="pRHBSTW-00675_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4665..5724,1..47)
                     /locus_tag="HV263_23965"
                     /pseudo=""
     CDS             join(4665..5724,1..47)
                     /locus_tag="HV263_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701154.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /name="DUF262 domain-containing protein"
     gene            46..717
                     /locus_tag="HV263_23970"
     CDS             46..717
                     /locus_tag="HV263_23970"
                     /inference="COORDINATES: protein motif:HMM:NF037890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLV85448.1"
                     /translation="MSNDPKDDFQNFVQSDLGWRKREMSTIKGFVFSSTGLNQHTYVRA
                     GIALLYSHWEGHIKQCSCEYINYLNKLGVKLSSLSENNITSYMYYKFSLLNGSKQITSY
                     TDIYNSISNNLTAEVFNIPPKSIIRTESNLKVRRLEDILHVIGIDNSAFMACKTQIDSQ
                     LLHHRNGISHGERTDGLQSTTLSTASYSTLHDKIIELIGLFSDAIIDHVINDKYMKQTN
                     P"
                     /name="hypothetical protein"
     gene            964..1290
                     /gene="mobC"
                     /locus_tag="HV263_23975"
     CDS             964..1290
                     /gene="mobC"
                     /locus_tag="HV263_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="QLV85449.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRR
                     RRVPDVAPELLRQVSGIGNNLNQIARRLNQTDTLTPAERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="mobC"
     gene            1280..2128
                     /locus_tag="HV263_23980"
                     /pseudo=""
     CDS             1280..2128
                     /locus_tag="HV263_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635827.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2514..2747
                     /gene="mbeD"
                     /locus_tag="HV263_23985"
     CDS             2514..2747
                     /gene="mbeD"
                     /locus_tag="HV263_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QLV85452.1"
                     /translation="MTEMEKQLLSALESLQTGYEQQQQAWQNSYSSLQHMFEATSQALT
                     HSDQVCQHLSSQVESLRAQVESLSRNVSRLMR"
                     /name="mbeD"
     gene            2912..3244
                     /locus_tag="HV263_23990"
     CDS             2912..3244
                     /locus_tag="HV263_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QLV85450.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKVRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAALKMLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3246..3530
                     /locus_tag="HV263_23995"
     CDS             3246..3530
                     /locus_tag="HV263_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QLV85451.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="helix-turn-helix domain-containing protein"
     gene            3722..4543
                     /locus_tag="HV263_24000"
                     /pseudo=""
     CDS             3722..4543
                     /locus_tag="HV263_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(1099..1290)
                     /name="Col(Ye4449)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.474"
                     /coverage="99.0"
                     /evalue="1.43e-39"
     Typing          1281..2848
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.455"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atagatggcc tcgattccaa gatttaataa aaaggatctt tttagatgtc taatgaccca
       61 aaggatgact ttcaaaactt tgtacagtct gatctcggtt ggcgaaaaag agaaatgtct
      121 actataaaag gttttgtctt ttcgtctaca gggcttaatc agcatacgta tgtgagggct
      181 gggattgctt tgctatattc tcactgggaa gggcatatca agcagtgctc atgcgaatat
      241 ataaactacc ttaataagct gggcgttaag ttaagctctt tgtctgagaa taatattact
      301 tcttacatgt actataaatt ctcactcctt aatggctcaa agcaaataac atcatatact
      361 gatatatata actctatatc taacaaccta accgcagagg tttttaatat accacctaag
      421 tctatcatca gaacagaaag taatcttaag gttagacggc tagaagatat acttcacgtc
      481 attgggatag acaattctgc ttttatggcg tgcaaaactc agatcgatag ccagctgctt
      541 caccatcgga atggtatatc ccatggcgaa agaactgacg ggttgcaatc gaccacactg
      601 agcacagcat catatagcac tcttcatgat aaaattatcg aactgatagg actattttca
      661 gacgcgataa tagaccatgt aataaacgat aagtatatga agcagactaa cccttaagtc
      721 gtcttttgca gagtgttcat aaacaaccta tatcagataa cagcccttca tcagcaaagg
      781 gctttttttt tgcagccagc agagaatctg acatactaca tgttactaca agccagtaca
      841 cacagcgtcc cctcttcgag gtgccgtctg tgactggttc agggggctcg ccgccccccg
      901 aaacccctgg catccactgc gaaaaattct cacggtggtg cgaatttttt ctcagcggag
      961 cctatggaaa agcgaacgaa agaaatcaaa atccggctca ccgaaaccga gcatcagcga
     1021 ctgcttgatc gctgcgaccg gactcacctt gcggagtggc tgcgtcagct tggtctgggt
     1081 gaacacactt cacgcaggcg ccgtgtgccc gatgtggcgc ccgaactgct gcgacaggtc
     1141 agtgggatcg gtaacaacct gaaccagatc gcccgccgtc tgaaccagac tgacaccctg
     1201 acgcctgctg aacgtgcctc gctgcttgtc gtactgacca gccttgaccg tcagcttgga
     1261 gacctgctgg agcagaaccg tgatcgttaa aattcactca cgcggcgcgg gttccggcag
     1321 tggcccagtg gattacctgt tgggtaaaga ccggcagcgc gaacaggcca gcgtactgcg
     1381 tggcaaccct gaacatgtgc gcgagctgat cgacggctgc gactttgccc gcgcctacac
     1441 ctccggcgtg ctgtcattcc aggaacctga catcgcagac gcggagaaat cgcgcctgat
     1501 ggacgaatgg gagcacacgc tgctgaccgg cctcgaccgc gaccagtacg cctgtctgtg
     1561 ggtagagcac cgcgacaagg gacggcttga gctgaatttt gtcatcccga acatcgaatt
     1621 gcagagcggc aaacgcctgc aaccctactt tgaccgcgcc gaccgtcccc gcgtcaatgc
     1681 ctggcagacc ctgaccaacg accggctggg gctgcgcgac ccgaacgatc cgacttaccg
     1741 ccgcccgctg acgcaggcca gcgatttgcc ccgcgacaaa cagcaggcgg cagagaaaat
     1801 caccgccggc ctgatgaatc tgatgcagca gggcgtgatc cgcagccggc aggacgtagt
     1861 gacgcagctg gaaagctacg gcctgaccgt ggcgcgggaa acgaaaagca gcatcagcat
     1921 tgccgatccg gatggcggcc gaaatatccg gctgaaagga atgatttatg agcgagattt
     1981 taaatttggc gaagggcttc gaggagaaat cgaagcagca ggcgcaggat accgagcaga
     2041 gcgtgaagcg cgagttcgcg aggctggaga cgtctatcag cgcggaactg caatcaagct
     2101 cgcagagcat cagcaacgct atccgcgagc agaacggcag gctgacgggc atgatcagaa
     2161 cctccgtttc aacggcgatg gcgtgggtat tcctctgctt cgccctgctg atcggcatcc
     2221 tgagcgggat aatctggttt cagggcacga tcatcacctc acgcctgagc gaaatggaca
     2281 gctacagcca gcagctggcg agcctgaaag cgaaaagcgg cgcgggggtg acgatttaca
     2341 gcgacgacac gcgccggaac acctacctgg tggtgctgcc gaagcaggct cacggtgtgg
     2401 aaacctacac gtcgacgacc gacaacaccg tggtgaaata cacggcaaaa taaccccgtc
     2461 agagcgccct gaaacgccac agcgcgtttt taacaccgag gacgaccaaa accatgaccg
     2521 agatggaaaa acagcttctg agcgccttag agagcttaca gacaggctac gagcaacagc
     2581 agcaggcgtg gcagaacagc tacagcagct tgcagcacat gttcgaggct acctcgcagg
     2641 cgctgacgca cagcgaccag gtgtgtcagc acttgagcag tcaggtagag agcttgcgcg
     2701 cgcaggtcga gagcttgagc aggaacgtca gccgcttgat gcgctgatca ggcagcacga
     2761 ccgcgcctac gccgagaagc tgcgtgagca gcagcagcca gagccggagc cgcagaaaaa
     2821 accgacccgc tactatggcc cctcgatgtg acggcttttc cggaatcaga attaagtgta
     2881 ctatgtactc cattaaacac aggagacagc cgtgagtcac gcgattgagt ttatcgagac
     2941 cccgatgttt acccgtcaga taaaacagat cgccacggat gaagagctca gggagctgca
     3001 aaaaacgctg attgagtcgc cggacaaagg cgatctcatc cagaaaaccg gcgggctgcg
     3061 aaaagtcaga atggcgacgg gcaaccaggg aaaaagcggc agcgcgaggg tgatttactt
     3121 cctggccacc gccgaggtga tttatctggt gatggcctat ccgaaaagca ccaaagacag
     3181 cctgacggac gcggagaaag ccgcgctgaa gatgctgacc caacaactga aagacgaggt
     3241 gtgacatgag tttttttgac gagctgaaag catctctgga agaagccgtt gaaattaaaa
     3301 acggcgtcag agagcctgcc cgcgtcactc gttacgaact ggcagacgtg aaggccatca
     3361 gggcacagct gaacgtatct cagagcgaga tggcaaaagt gctgggaacc agcctcgaca
     3421 ctatcaagag ctgggaaacc ggacgccgta acccgaccgg actggcggcg aaggtgctgg
     3481 caaccatcca ggcgaacccg aaattcttcc aggagctggc ggcgcactaa gccgttgtga
     3541 cagaaggatt cgacaggcga ggccgttcgc gtgtaattta acggcaacag aaaaaacaaa
     3601 gccccggcaa tcatgagcta tcttggcgga tgtgcatgat taccagggcc aggtcgaaac
     3661 ctaaaaggat attagcacat gaaccgtgcc agacaacagc ccgccataag cgcagggcgc
     3721 attgcataac gccgcactcg aactcttcaa cgaacgctta ccgcacaagc cgtacttttc
     3781 ggacgatctg cactttggtg tacgcattgc cggtaaggaa cgcgccattc tggcgaaata
     3841 cattcagttc aatcagcctc acgctatgtt ttggctgggc ttcgacgtcg atcgcgccgg
     3901 tgccgccatc gactggagcg accgtaacgc ccccgcgcct actctgacca tcaccaatcc
     3961 cgaaaacgga cacgcgcacc tgctgtatgc actgaaaacc tcgatacgca ctgcgccgga
     4021 cggaaaaatg aagccgctgc gctacgccgc cgccgtggag aacgcgctac gtaaaaaatt
     4081 ggaggctgac gcagggtatt cggggctaat ctgcaaaaat ccgaaccatg gccactggaa
     4141 aattgccgta tggcagccag aactctacac actggactgg ctggctgact ttctcgacct
     4201 gaacgccgcg aacgacaaag aaatcgttgc cgactacggc ttaggccgta actgcacgtt
     4261 gttcgacaaa acccgcaagt gggcataccg cgccatccgt cagggctggc ctgagtacga
     4321 ccagtggcta aaagcctgct acgaacgtgc cagcgcctat aacctgcaat tcgcagcgcc
     4381 tcttgatgaa aatgaagtaa ttgggatagc taaaagcata gctaagtgga caagtagcca
     4441 ttttaatgaa acttctttta aagagtatgt aaaacgaacg cacactaaaa ttatgcaatc
     4501 ctctagaggg aaaaaaagcc atggtggagg gagaaaaaaa atatgttctg ataaagtatt
     4561 ggagataaag aaaatgatat cagatggaaa ttcttaccga tacgtctctg gaaaatgctc
     4621 agtttctatt ggtttcataa gtaaagtaat aaaggaaaat taagatgggc gatcttgtta
     4681 gtcaaattga gaaaagaaga aaggaaattt ttactgattc ttatcctatg tcgataggtg
     4741 agttagtcaa cctctataaa gaaggtgaga tagatataaa tccatcattc caacgatttt
     4801 tcagatggtc tcacaaacaa aaagtaagtc ttattgagtc aatattacta ggaataccta
     4861 tcccatctat tttcgttgct caaagagatg atggtgtttg ggatcttgtt gatggtcttc
     4921 aacgggtgtc aacaatttta ggattgatgg gggagttaaa aaatgatgct ggagaaacgt
     4981 tggagccact tttactcaaa gaaaccagtt atcttcctgc gttggaaggt aaggtttgga
     5041 acggtgacga aagtaacgag ctagataagt ctataaaaat ggcgtttaag agagagaaga
     5101 ttgaaataaa gataataaaa aaagaaagcg atgtaaacac taagttcgag ttattccaaa
     5161 ggttaaatac aggtggtagt gagctttctg agcaagaggt aaggaactgc ttgcttatta
     5221 tgttagataa aaacgcttat gataacttag atagtttatc taagtatcaa caatttgttg
     5281 attctatatc tataacggaa aggttgtttg aagagcgtta tgatatggag ctggcgctga
     5341 ggatgttcgt tctcggtaat gctgccggtg atgaccttaa aggtatagaa gaagtatctg
     5401 atttcctcac cacaaaagta actgacttta cttgcgataa attcgattgg gacgaggaat
     5461 ctaaattatt caggaagtca ttcgatatta tatatgaatc tttaggtgat aactcattta
     5521 aaaagtatga tttagttaaa ggtggctaca gagggggatt tcatctttct gcttatgagg
     5581 ttttatccac cggggtttat aaagcattga aggccgccgc tcttagtgat gaccagataa
     5641 aagaaattgt tgtagaacat tccagaaata ttttttctga cgaagttttc ataaaacaca
     5701 gcggttctgg agcaaaggca aatt
//
