LOCUS       CP056675                3293 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Klebsiella oxytoca strain RHBSTW-00651 plasmid pRHBSTW-00651_8,
            complete sequence.
ACCESSION   CP056675
VERSION     CP056675.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148673
KEYWORDS    .
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00651
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77.9763304491051x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 22:10:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,737
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,526
            CDSs (with protein)               :: 5,526
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 32 of 95
            Pseudo Genes (incomplete)         :: 60 of 95
            Pseudo Genes (internal stop)      :: 14 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00651"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:571"
                     /plasmid="pRHBSTW-00651_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(167..697)
                     /locus_tag="HV256_28670"
     CDS             complement(167..697)
                     /locus_tag="HV256_28670"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="QLU07930.1"
                     /translation="MRLIPGFQPFDLCAEYSMANFSCGVTHLDEFLKTDLHKMQGRSLL
                     KGYVLLSDDEIPEIMGYYTLSGASFERGHLSNRLRKKIPYSNMPAVLLGRLAIDQRIQR
                     KGFGELLIVDAIHKVRATAGQIGIYAMFVEAHPGASSFYTRMGFIPSSMADDNKTFFYP
                     ANQFDDLIFGDKC"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(688..972)
                     /locus_tag="HV256_28675"
     CDS             complement(688..972)
                     /locus_tag="HV256_28675"
                     /inference="COORDINATES: protein motif:HMM:NF020265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="QLU07931.1"
                     /translation="MNAVRKERFEFRLPEAAKRRIEEAAVISKVSVSQFVMESATFRAE
                     TVINEHRRLIVEEDVWEQVMAALERPPAPTQSMCKAFERYNSEESWICD"
                     /name="DUF1778 domain-containing protein"
     gene            complement(2125..2547)
                     /locus_tag="HV256_28680"
     CDS             complement(2125..2547)
                     /locus_tag="HV256_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QLU07932.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVID
                     TSELIRIFGSLYGGEDTVKHDVNGDAGATAVNILGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2547..2738)
                     /locus_tag="HV256_28685"
     CDS             complement(2547..2738)
                     /locus_tag="HV256_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLU07933.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNEFDLDIEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2787..3268
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.858"
                     /coverage="92.0"
                     /evalue="1.31e-148"
ORIGIN
        1 gcgccggtta aggctaaact gaaaggactg atttggtgac tagctcctct ttagccagtt
       61 accttggttt agtgaacttg ccggttcacc aaccttaaaa aagccacctt gccgggtggt
      121 ttttttttac tttcacatat atctcataga tatatgtgaa agtactctag catttatcgc
      181 caaagataag atcatcaaac tgattagctg ggtagaaaaa tgttttatta tcatcagcca
      241 ttgatgatgg aatgaatccc attcttgtgt aaaaagatga agctccagga tgcgcctcaa
      301 caaacatggc atagatacca atctgtccag cagtagccct cactttatgg atagcatcga
      361 caattagtaa ctcaccaaaa ccttttcgct gaattcgttg atcgatagca agacgaccta
      421 acagcactgc cggcatgttc gagtaaggaa ttttcttcct caggcggtta ctcaaatgcc
      481 ctcgttcgaa cgatgcaccg ctcagcgtgt agtaccccat aatttctgga atttcatcat
      541 cagaaagcag aacgtagccc tttaataggc tacgcccctg cattttgtgg agatcggttt
      601 tcaaaaactc atctaaatgt gtgactccac agctaaagtt cgccatgctg tactcagcac
      661 acaagtcaaa aggttgaaaa ccagggatta atcgcaaatc caactctcct cagagttata
      721 acgctcaaac gctttacaca tactttgtgt tggtgctgga gggcgttcaa gggcagccat
      781 gacttgctcc caaacatcct cttcaacgat caagcggcga tgttcattaa taactgtttc
      841 agctctaaaa gtcgcagact ccattacaaa ttgagatacg gacactttag aaataacagc
      901 agcttcttca atacggcgct tcgcagcttc gggcagacga aactcgaacc gctcctttct
      961 tactgcgttc ataacaactc ctgagctaat gctcacagtg gtttatacca catgacgata
     1021 tgcacggtac ggtaatttac cgtacaggta atttgaggtg attttgatct gagaaactat
     1081 gccagtcaag ctaatgaagg gtgagggtag tattttttga gaaaaaaact taaacttcgg
     1141 aaaaataacc ttaaaaaaca acaacttaac catggcaatg acacttagga ggcgttagtg
     1201 gttcttaaga accactaacg cctcctaagt gtcattgcca gtttatttta tcattttttt
     1261 gcatgtaaat ctaccgctat tttgaacgaa tgtatgggcc aaatcacaca gcattcaggg
     1321 gatgtgcatt acggtatttt gcgccccata aatccaccca cgcactccaa aacaagtcac
     1381 tgatccagat ttaccataac gtctcattac gcgcaccgct caaacgctcc agaaacgatt
     1441 ctggggcgta acccattaaa tcctacgtct taccccctga aaacgcctca aaatcgcgct
     1501 gatgggtttt acgggtatga attgttatgc accagactgg ctggaggcag atttcatcct
     1561 gaattcacgc cagacaactc aaaacccgca ccaaacccga ctgaaaacgg tgaacgggga
     1621 cgcaatttat tgcgtggccg tgaagcgttg ccggcgcagc cggaaaaccg gttttcagca
     1681 cgttcaggaa tcgagttcgt cgacgcccgg caatcacgct atggcaagtc gccggacgac
     1741 tcaccacacg attcaccggc gactcttcac tagagtcgta cctagagtcg ccgagtctaa
     1801 aaaaaaccaa ccggggagtc gggtctagag tcgggtcgtt attaactcat tgtttttata
     1861 gtcctgagtc gttaccgact ctatggcgac tcgccgggga ctctatgacg actcgtcata
     1921 gagtcggatc tatgtaaatg aaatttaaaa aacgaaatat caggaaaata aactactgat
     1981 attaaataca aatctgcaaa aatttaattt tgcgtagtgt gtgggtattt ttagtggtga
     2041 ggagcgttca gagatctgac gatttttggg aaggttcagg aactccacgg gatggccgga
     2101 aaggaaaact gaagcatgac ggggtcagga ttttttccag aatttccacc acggagaatg
     2161 aacttgtgga gagggttcgt gcttgtgttc cagaagctgc atagcctggc gaagagaatc
     2221 gatatgttca tcacgagctt taacagcccc tttaagacca tcgttttcag cttcaagcaa
     2281 agcaatttta tgctgtaaat catgtaaacc aaggatgtta accgctgtag cgcctgcgtc
     2341 accgtttaca tcatgtttta cagtgtcttc cccaccatac agcgagccaa aaatcctaat
     2401 aagctctgat gtatcaataa ctttagtatt tttcccatgc tttacaacag aggtgtaact
     2461 tagtttccca ctgttaatca tcttgtaaat tgtggggcga ctaaccttaa caagccgtgc
     2521 cgcttcacta atagacactc tggccatcag ttaccatcct gtaattcatc cagtctcgag
     2581 gacagtgtac ggaaaagatg ctccgcatca tcgtggagtt tctcacaaag gtcggcctcg
     2641 atatcgagat caaactcgtt aagcttctcc agcagaagca gagactgatt ctggatgaat
     2701 ttagccatat tcagcgctgt tttctgttgt ttagtcataa agggcctcag agcgtcagtg
     2761 ctcagtgtca aaccagtctc ttttccgctt cctcgctcac tcagtcgcta tgctcctgcc
     2821 gttcgactgc ggcgagcggt cgcaactcac gtataagggc atgagacgag agcgcggata
     2881 gccagagagg acaaggagaa ggccgcggcg ttgccgtttt tccataggct ccgcccccct
     2941 gacaagcatt acagaaacaa acgctcaaat cagtggtggc gaaacccgac aggactataa
     3001 agatacctat catttctgat aggtggctcc ctcgtgcgct ctcctgttcc tgccattcgg
     3061 tttacccgtg tcattccgct gttatggccg cgtttttctc attccacgcc tgacactcag
     3121 ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga
     3181 ctactgcgcc ttatccggta actatcgtct tgagtccaac ccgaataggc acgacaaagc
     3241 gccactggca gcagccactg gtaactgatt agaggagtta gtcttgaagt tat
//
