LOCUS       CP056674                3677 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Klebsiella oxytoca strain RHBSTW-00651 plasmid pRHBSTW-00651_7,
            complete sequence.
ACCESSION   CP056674
VERSION     CP056674.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148673
KEYWORDS    .
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00651
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77.9763304491051x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 22:10:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,737
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,526
            CDSs (with protein)               :: 5,526
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 32 of 95
            Pseudo Genes (incomplete)         :: 60 of 95
            Pseudo Genes (internal stop)      :: 14 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00651"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:571"
                     /plasmid="pRHBSTW-00651_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            191..1321
                     /locus_tag="HV256_28655"
     CDS             191..1321
                     /locus_tag="HV256_28655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU07927.1"
                     /translation="MEFKIKKMTVKDSREIKKKFEDCIKIAEEKNMQVGSILNIEMQDR
                     LKEINLKVKKETAEQYRKLFNYKLSVNAEDIIQHLLECNTRNSFDKTRSAFRFCIAEKI
                     QELRNEADRARKQKNFDLMKEKTIEAYKLFFFFERDFLSENRVTWNDISYRKIDSKSKK
                     KTMNSVSSIKAIFEDLKQKPELFDKYSLILAISSLTGCRPAEIQKGIGLELSPPLLNIC
                     ISGAKFGEDRGQEKRILKFNINEFAENEQFKLIMSKIKQNNKLEYKCSKQEYDSLRQYL
                     YINHKGFSLYTLRHRAASELKKEGFTEETIAAFLGHRVTRSLENYGYARSGKGGPKIAG
                     VEHTNAIKSNTKRYKSKTTPGGGNPSSTRKRALKRF"
                     /name="hypothetical protein"
     gene            2479..2727
                     /locus_tag="HV256_28660"
     CDS             2479..2727
                     /locus_tag="HV256_28660"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU07928.1"
                     /translation="MEIEKLIDRYFLALERKVIREIYDKQTREELEKFIFKCNGEFREF
                     ERRFNTICSTEYYEQQSLIFYINYINAEFEQREKLNY"
                     /name="hypothetical protein"
     gene            2793..3494
                     /locus_tag="HV256_28665"
     CDS             2793..3494
                     /locus_tag="HV256_28665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU07929.1"
                     /translation="MTISKKNSELRERFKEYSSNKNIFDLADLRYEILKIYFDFKLKND
                     MNEQERKSQDSRRKAHLTALKKRIKREIVSKIVIDLVKYYNIEKTTFHFFSHICTEILE
                     RNVDNRYILNNFSNMILDEKKELTKLSESRNASNKMSLENSYSELVSMSHIKDKLFRND
                     NFKTAYLKCYGCANEEFSRFKVFAFPDNFETLDFLFEEERIKKEEKEISKIMIEQVEEE
                     PKIQPIKKRRL"
                     /name="hypothetical protein"
ORIGIN
        1 gtcagcgaaa ttttgaaagg aaaagtgtgc gaagagcaca aaaccctttt gctttttttc
       61 gtaatgacat tttaaaaata tttcattttg agcgaagcga aatttgaaaa tttttaattt
      121 ggaatgaaga aaaaaggcaa accagcagag tgaaaataaa acaataaaac tataaaaaaa
      181 agaggtggct atggaattta aaataaagaa aatgactgtt aaagacagca gagaaattaa
      241 aaaaaaattt gaagattgca ttaagatagc agaagaaaaa aatatgcaag tcggttcaat
      301 tctaaatatt gaaatgcaag atagattgaa agaaataaat ttaaaagtaa aaaaagaaac
      361 agcagaacaa tatagaaagc tgtttaatta taaattgtct gtgaatgcag aagatattat
      421 tcagcacttg ttagaatgca acacaagaaa cagttttgat aaaacaagat ctgcttttag
      481 attttgcatt gctgaaaaaa ttcaagagct aagaaatgaa gcagatcgag caagaaagca
      541 aaagaatttt gatttaatga aagaaaaaac tattgaagca tataaattat ttttcttttt
      601 tgagcgagat tttttgagcg aaaaccgggt aacttggaat gatatttctt atagaaaaat
      661 agactctaaa agcaaaaaaa agactatgaa tagtgtttcc agtattaaag caatttttga
      721 agatttgaag caaaagcctg agctttttga taaatatagt ttgatcttgg caatttcaag
      781 tttgaccggg tgtagaccgg ctgagatcca gaaaggcatt gggctggaac taagcccacc
      841 actattaaat atatgtatta gcggggcgaa atttggagag gatcgaggac aggaaaaaag
      901 aatattgaaa ttcaatataa atgaatttgc cgaaaatgag cagttcaaat tgattatgtc
      961 aaaaataaag caaaataata aattagaata taaatgctca aagcaagagt atgattcatt
     1021 gagacaatat ttgtatatca atcataaagg tttttcactt tatacattaa gacacagagc
     1081 agcgagcgaa ttaaaaaaag agggttttac agaagaaaca atagctgcat ttttgggtca
     1141 cagagtaact agatctctgg aaaattacgg ctatgcaaga tctggcaaag gtggtccaaa
     1201 aatcgcaggt gttgagcaca caaacgcgat taaaagcaat acaaagcgat ataagagcaa
     1261 aaccaccccc ggagggggaa acccttccag tactcgtaaa cgagccttaa aacgatttta
     1321 gagagttaca gagccaacat tctgttggct tttcatatgt gtgttagcct cgcgcgcgcg
     1381 tcttgccggc acagatttgt ggtgactggt tgcatatgtg tattagcaga accacagatc
     1441 gctgtggttc ctggtgtggt cggtgtaatt aactaatcaa tatatgtatt agcgggtagc
     1501 tcgcgcgcga gtagtgcccg gaacgggacc ccggtaactt tcgttggtgg ttttttgagc
     1561 aattaactgt cagtcgttga catttgaagt gtcatttttg ctgttaaaaa tgacacaacg
     1621 agtctcattg agtgagaatt ttgatttttg agcaatcaaa ttttttgaag aactattgac
     1681 agaacaagcg gataagttat aactgaagcc agatacaaca aaaccgactt tcgtcggctt
     1741 tgagggactt aaagaagtgg ggacttctga aagtcttgta atcaagtgaa atcagcacaa
     1801 gattacgata tgtaagtaaa aatctaactt ataaaatcgc aattgtcaaa cttctcttct
     1861 tacaagtctt ttcaaaaaaa cccatctttt caagttctta agcacatata ccattaagaa
     1921 acctattgta tcaggatcag attgtggagc cgttctatgc gagtcataga catgtgattg
     1981 ttcgatctgg tgtatgtgtt gcgataaacg gggagtgcta caaaatcgca aaccgggaac
     2041 gaatatctaa gttgatttgg taactgtgca gttagtgagg aatgagaaaa cgcgacttac
     2101 agcggatctg acagtgcaag taatcaatat gatatgacca ggggggtgta gcagggtgcc
     2161 gcaaagaggg aaacctctcg cagctctaag tcctggctct ttgggtgaag tccatttttc
     2221 accaaaggat cgtcaacttg attagcctta accataggca atcaaggagg cgaaactttg
     2281 ggtgaagccc aaagaacaaa gggctggccc ccccgaaaag caatacctgc aaataatatt
     2341 gtgcgaagca ttagagaaat atagaaaaac aatataaaga aaaaaagaaa tagagttcga
     2401 agaaaaaaat tcgaaatttg attttttttt gcaatttgct atttaaattc caaataacat
     2461 ataaaaaaag gaaaacttat ggaaatagaa aaattgattg atagatattt tttagcatta
     2521 gaaagaaaag tgatccgtga aatttatgat aaacagacac gagaagaatt agaaaaattt
     2581 atttttaagt gtaatggtga gtttagagag tttgaaaggc gatttaatac aatatgctct
     2641 actgaatatt atgaacaaca aagccttatc ttttatatta attatataaa tgcagagttt
     2701 gagcagaggg agaagttaaa ttattaaatt tgacaacatt aaaataatat attaagcata
     2761 tatgtattag cgaaacacag agggcttaat atatgacaat atctaagaaa aatagcgaat
     2821 tgagagagcg atttaaagag tattcaagta ataaaaacat atttgatctt gctgatttga
     2881 gatatgaaat attaaaaata tattttgatt ttaaattaaa aaacgatatg aatgagcaag
     2941 aaagaaaatc tcaagactca agaagaaaag cacatttaac agcattaaaa aaaagaatta
     3001 aaagagaaat cgtttcgaaa atagttattg atttagtaaa atactacaac atagaaaaaa
     3061 cgacttttca ttttttttct catatatgta ctgagatttt agaaagaaat gtagataaca
     3121 gatacattct aaataatttt tcaaatatga ttttagacga aaaaaaagag ttaacgaaat
     3181 taagtgaaag cagaaatgct tcaaacaaaa tgagtttaga aaactcttat tccgaacttg
     3241 tttctatgtc acatattaaa gacaaattat ttaggaatga caatttcaaa actgcatatt
     3301 taaaatgtta tggttgtgca aatgaggaat tttcaagatt taaagttttt gcttttccag
     3361 acaattttga gacattagat tttttatttg aagaagaaag aataaaaaaa gaagaaaaag
     3421 agatatctaa aataatgatt gaacaagttg aagaagaacc aaaaatacag ccaataaaga
     3481 aaagaagatt ataatttttt tttgcggcga atcgtgagaa aaataaaatt ttaattttgt
     3541 tttttgagcg attgatctgt atgtaagttg atgaaaaatc tagtaaaatc tgcgatctat
     3601 tttttctttt gacttgccat tattgttatt tttgttataa caataatggg gggttgtctt
     3661 ttacttgaaa aatgagc
//
