LOCUS       CP056673                5177 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Klebsiella oxytoca strain RHBSTW-00651 plasmid pRHBSTW-00651_6,
            complete sequence.
ACCESSION   CP056673
VERSION     CP056673.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148673
KEYWORDS    .
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00651
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77.9763304491051x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 22:10:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,737
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,526
            CDSs (with protein)               :: 5,526
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 32 of 95
            Pseudo Genes (incomplete)         :: 60 of 95
            Pseudo Genes (internal stop)      :: 14 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00651"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:571"
                     /plasmid="pRHBSTW-00651_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(98..1195)
                     /locus_tag="HV256_28630"
     CDS             complement(98..1195)
                     /locus_tag="HV256_28630"
                     /inference="COORDINATES: protein motif:HMM:NF015354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QLU07922.1"
                     /translation="MAIFHLSFKVLTRTTKTGKSKSSLYLAAYNSREKLKDELTGLTFD
                     YTKKNDLYKSGIILPEDAPSKFYDRATLWQNCEASETRRDSQICRYFIVALPQEQNGEQ
                     NEALLKKYLKDNFVKLGMCADYAIHDINNKPHAHVMLTMRKCENGGFSKKKAREWNDPK
                     LAEVWRRQWSVLVNKTLRENGHKNTITHLSFLRQKELAIKKAHEALENNDIKKAEELIN
                     VAKKLATKKPVKRKPTVKFLKDKARKKNKNPELRHRLKQQQEQKEENIKNNKSVGLFFR
                     NILLKLKGGNDKKNKTEKTQKMTEADFLKNMKTDEEEAENLELNKYIEEELKNGINKKN
                     DITHKPPRRGVGSRKEKNNKPQGRK"
                     /name="MobA/MobL family protein"
     gene            1572..1940
                     /locus_tag="HV256_28635"
     CDS             1572..1940
                     /locus_tag="HV256_28635"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU07923.1"
                     /translation="MGKSEIDILAEELKEFYFDALGENNGRVFAYRATYKVKGWEQIEQ
                     KAFRNAFFKFFKTDAQLRKTKDIKSDYFQLEGIKDKFNRYYFPSFCIDKKEYEARGVEY
                     LKEVEEYFKKLITLAAIK"
                     /name="hypothetical protein"
     gene            2060..2356
                     /locus_tag="HV256_28640"
     CDS             2060..2356
                     /locus_tag="HV256_28640"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU07924.1"
                     /translation="MSLQQQKERLKRELAALEKKEKHLKDQANAKNMKAIAAEILKYDL
                     EGYDNFKKVVGLLAYFDYQDEDFKNGLMKWAENELEERKAKKVARTFKKKPQK"
                     /name="hypothetical protein"
     gene            2378..2923
                     /locus_tag="HV256_28645"
     CDS             2378..2923
                     /locus_tag="HV256_28645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU07925.1"
                     /translation="MDKLTLLDMFKHGTATWSAVSSFVVVLFWCVGCWLSFLAIVNYKN
                     AADGKSGIAKPIIQTVIAALMVAISRFIPILSDTLNNKAAEFSPQSLLSDIPTTDPMGL
                     GLAFTSVLLFVQMLGTIALFRGFMMLWEATNKGAGSGLIGKSWTHIIGGVLAVNIQLTI
                     ATVASTFYPGVDLSFLGL"
                     /name="hypothetical protein"
     gene            3583..5130
                     /locus_tag="HV256_28650"
     CDS             3583..5130
                     /locus_tag="HV256_28650"
                     /inference="COORDINATES: protein motif:HMM:NF013603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QLU07926.1"
                     /translation="MTKKKNPVTFELRATKENGINQMLYENNKAYGDIVNTGEVPFLTT
                     YAAQNGQRLNERKGKSIRLAKLYYLQADNLEGGKADAMRKRAGRVFGCCSHREMAIGSG
                     GYGYNLYSHRCKDRNCVECQRVRAFILQQKIREITPQLIAQTNAKDGLIFGTLTVKNPL
                     ITELKDYLKIMSKAFARLMRRKEFNKIGVGGFRCFEVTRGESGADFCHPHIHFLLQVKA
                     SYFARNSPLYINADQWATAWTDCLKLEVEKTGRVFNVADYPNGKAFVKILRVQAPDYTR
                     ENRKYATIETLQKEGDQVINYVLKYTAKEDENSKKALVKNDPWFFEYDKQIKNIRAISF
                     FGIYKKLISELPPREYNEEEIRKDLNNNQAKFYSVMWDDDHKYIAIETTEEEALNKKRT
                     NTINSIKTTLIAQLESKNYILDIMIAAMNKGDFLTVNEEIKNHNILADRTYKTFKRMEK
                     AGELVEGKNGFYQPFHDYLFKSVIYEKMESILSAEELENIKKEIELNIEVIEDNPPF"
                     /name="protein rep"
     Typing          complement(117..1195)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.833"
                     /coverage="99.0"
                     /evalue="9.88e-163"
ORIGIN
        1 gttttttgct ctttcctttt gttcgtgttc ctcttctttt tttgctgata attcagcgtc
       61 tggatcacgt aaaaacattt ctataatcca tcttttatta ttttcgtccc tgtggcttat
      121 tattcttttc cttcctactt ccaactccac gcctcggtgg tttgtgtgta atgtcatttt
      181 ttttattaat cccatttttt aattcctctt caatatattt atttaattct aaattttcag
      241 cctcttcttc gtctgttttc atgtttttta aaaaatcggc ctctgtcatt ttttgcgtct
      301 tttccgtttt gttttttttg tcattccctc cttttagttt taatagaata tttctaaaaa
      361 acaatcctac gcttttatta ttttttatat tctcttcttt ttgttcttgt tgttgtttta
      421 atctatggcg caattcggga tttttatttt tcttacgtgc tttatctttt aaaaatttaa
      481 ccgtaggttt acgcttcacg ggctttttag ttgctaattt tttagctacg tttattaact
      541 cttcagcctt tttaatgtcg ttattttcta atgcttcatg tgcttttttt atagcaagtt
      601 ctttttgtcg taaaaaactt aaatgcgtta ttgtgttttt gtgtccgttc tctcttaatg
      661 ttttattaac taatactgac cactgcctcc gccatacttc cgctaatttc ggatcattcc
      721 actcgcgcgc ctttttttta ctaaatcctc cgttttcgca ttttctcatt gtaagcatta
      781 cgtgggcatg tggtttatta tttatatcgt gaatagcata atccgcgcac attcccaatt
      841 ttacaaaatt atcttttaaa tattttttta atagcgcctc gttttgctcg ccgttttgct
      901 cttgtggcaa ggccacgata aagtacctgc agatctgact gtcccggcgt gtttctgatg
      961 cctcacaatt ttgccatagt gtagccctat cataaaattt actaggggca tcttctggta
     1021 aaataatacc gcttttatat aagtcatttt ttttagtgta gtcaaaagtt aaacctgtta
     1081 actcatcttt taacttttcg cggctgttgt aagcagccaa atataatgag cttttgcttt
     1141 ttcctgtttt tgttgtacgc gttaatactt taaaacttaa atgaaatatt gccacggctg
     1201 gcctcctttt gtttttgttt ttttttatgc ccacacacac ggaagagggg agggcatgac
     1261 ggcgggggca acctttgcga agcattggga agccttgcgc cgtgcggtgc gcgttagcgt
     1321 tccgcttgtc gcttgcgaca aagggcgaag ccctaaagcc tcgcagagac cgcacgttgc
     1381 cgaaggcaat gtataagcgg gcatttatca ctaacgttct aaatgtggct tacttcgtgc
     1441 gcagctaccg ccttttttat tatttaataa aaccttttaa aaaaaagtaa ataaaaccaa
     1501 ttaaaaattt gacatttatt ttttatatat taaagaaaaa atataaaaga aaaaaaagag
     1561 gggtttaatt tatgggaaaa tcagaaattg atatattggc agaagaattg aaagaatttt
     1621 attttgatgc gctgggtgaa aataacggtc gtgtttttgc ttatcgagca acctataagg
     1681 tgaaagggtg ggagcaaata gaacaaaaag cttttagaaa tgcgtttttt aaatttttca
     1741 aaacagatgc tcaattaaga aaaacaaaag atataaaaag cgattatttt caactggaag
     1801 ggataaagga taaatttaat agatattatt tcccttcatt ttgcatcgat aaaaaagaat
     1861 atgaggcaag aggtgttgag tatttaaaag aggttgagga atactttaaa aaattaataa
     1921 ccttggcggc tataaaatga ttataacttt tattataaat attattatta ttgtttcatt
     1981 atccgcgctt tacattgtaa agcgtaggca gtggtcaaaa aaaaagaaaa aacaaacatt
     2041 ataaaaataa gggggctata tgagcttaca acaacaaaaa gagagattaa aaagagaact
     2101 cgcggcgtta gagaaaaaag aaaaacactt aaaagatcag gcgaacgcta aaaatatgaa
     2161 agcaatcgcg gcagaaattt taaaatatga tttagaagga tacgacaatt ttaaaaaagt
     2221 tgtcggattg ttggcatatt ttgattacca agatgaagat tttaaaaacg gcttgatgaa
     2281 atgggctgaa aatgaacttg aagaaagaaa agctaaaaaa gtagctcgga catttaaaaa
     2341 aaaacctcaa aaataataaa taatgagggg gttaatcatg gataaattaa ccctgttgga
     2401 tatgtttaaa cacggaaccg ctacatggtc agcggtttcg tctttcgtcg ttgttttgtt
     2461 ttggtgtgtt ggctgttggc tgtcattttt ggcgattgtt aattataaaa acgccgcaga
     2521 tggcaaaagc ggaatcgcaa agccaatcat tcaaacagtg atcgccgctt tgatggtggc
     2581 aatttctcga tttatcccca ttctttcgga cactttaaac aataaagcgg cggaattttc
     2641 acctcaaagc cttttaagtg atatacctac aaccgacccg atggggcttg gtttagcttt
     2701 tacgtctgtt ttgttattcg tccaaatgtt gggaacaata gccttattta ggggcttcat
     2761 gatgctatgg gaggcgacga acaagggcgc aggtagtggt cttattggta aaagctggac
     2821 gcacattata ggtggcgttt tagctgtaaa catacagtta accatcgcga ccgtagcctc
     2881 gacgttttac ccaggcgtcg acctttcgtt cttgggtttg tgagtgtagg cgggtcaatg
     2941 gccagcttgc tgggcgtagc gaaccgttga cgcgacggaa cgaccccacc cttggctaag
     3001 ggggaaggag agccacggcg agccttcctc ctgttaagcc gcccttttgc cctttatact
     3061 caccgcagga cggcaggcgt ccgggtgggt ttgtttgttt cgtgtctgtc gtcctatccc
     3121 ttccctttct ctctggatct ggcagtcgca gggtttttgt cttttagccc cctctggggg
     3181 tttaaaatcc gttcaggatt tcgctttttg gtttttgtct tttaagcccc agcggggcgc
     3241 tctcaacttg attattaaga aataaatcgc acgaattttg gggggtaaat cgggctacaa
     3301 gccgcgccat tgctggattt ctttgttttt ttaattacat accaaaacgg aaaaaagcgc
     3361 aaaatttata cagggcaagg aggggctaaa agaaacgata aacgggcaac ataaagcgat
     3421 aaggggtaaa cctgggacgg ctctacattt ttaacactaa atagggggtg tttttgggac
     3481 ggcatacatt aaagcagtta aaaaacgggc tataaatggg acgggatcaa ccgttagggg
     3541 gatttgcgcg ggtgtttttg tttatggcga ttttttaagc atttgacaaa aaagaaaaat
     3601 cccgtaactt ttgagttgcg agctacaaaa gaaaacggga ttaatcagat gctttatgaa
     3661 aataataaag catatgggga tattgtcaac actggcgaag tgccattttt aaccacatat
     3721 gcagcccaaa atgggcaaag attgaacgaa agaaaaggca agagcattag gcttgccaag
     3781 ctttactatt tacaagccga caatttagaa ggcggcaagg ctgacgcaat gagaaaacgc
     3841 gcggggcgtg tttttggttg ttgtagccat agagagatgg ccataggttc gggcggatat
     3901 ggctacaatc tttatagcca cagatgtaaa gaccgtaatt gtgtagagtg tcagcgcgtc
     3961 cgtgctttca ttttacagca aaaaatacgg gaaattacac cgcaattaat agcacaaaca
     4021 aacgcaaaag acgggctaat atttggcacg ttaacagtaa aaaacccact tattacagag
     4081 ttaaaagatt acttaaaaat aatgagcaag gcttttgctc gcttaatgcg tcgcaaagag
     4141 tttaataaaa taggtgttgg cggttttagg tgttttgaag tgacaagggg cgaatctggc
     4201 gcggattttt gccacccgca tatacatttt ttactacaag ttaaagcgtc atattttgcg
     4261 cgtaattctc cattatatat aaatgctgat cagtgggcga cagcatggac tgactgttta
     4321 aaattagaag tagaaaaaac ggggcgcgtt tttaacgttg cagactatcc gaacggtaaa
     4381 gcatttgtaa aaattttacg cgtgcaagcc cctgattaca caagagaaaa tcggaaatac
     4441 gcgacgattg aaactcttca aaaagaaggt gatcaggtta taaactacgt tcttaaatat
     4501 accgcaaaag aagatgaaaa cagcaagaaa gcacttgtta aaaatgaccc ttggtttttc
     4561 gaatatgaca aacaaataaa aaatattcgc gctatttcat tttttggaat ttataaaaaa
     4621 ttaatatcag aactgccgcc gcgtgaatat aacgaggaag aaataagaaa ggatttaaat
     4681 aataatcagg ctaaatttta tagcgtgatg tgggatgacg atcataaata tattgcaatt
     4741 gaaacaacag aagaagaagc gttaaataaa aaaagaacta acacaataaa cagcataaaa
     4801 acaacattga tcgcacaact tgaaagtaaa aattatattc tggatataat gattgcggca
     4861 atgaacaaag gcgatttttt aacagtcaac gaagaaataa aaaatcataa tatccttgct
     4921 gaccgtacat ataaaacatt taaaagaatg gaaaaggcgg gggaattggt agaagggaaa
     4981 aacggatttt atcagccttt ccatgattat ttatttaaaa gcgttattta tgaaaaaatg
     5041 gaaagtattt tgagtgcgga agaattggaa aatattaaaa aagaaattga attaaatatt
     5101 gaggtaatag aagacaaccc accattttaa agtatattaa aaagccccta tattggggct
     5161 aatgtttatt ttattct
//
