LOCUS       CP056671                9509 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Klebsiella oxytoca strain RHBSTW-00651 plasmid pRHBSTW-00651_4,
            complete sequence.
ACCESSION   CP056671
VERSION     CP056671.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148673
KEYWORDS    .
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T2-00651
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 77.9763304491051x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 22:10:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,737
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,526
            CDSs (with protein)               :: 5,526
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 32 of 95
            Pseudo Genes (incomplete)         :: 60 of 95
            Pseudo Genes (internal stop)      :: 14 of 95
            Pseudo Genes (multiple problems)  :: 11 of 95
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00651"
                     /isolation_source="Wastewater effluent sample"
                     /db_xref="taxon:571"
                     /plasmid="pRHBSTW-00651_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(9355..9509,1..451)
                     /locus_tag="HV256_28550"
     CDS             join(9355..9509,1..451)
                     /locus_tag="HV256_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QLU07907.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            490..624
                     /locus_tag="HV256_28555"
     CDS             490..624
                     /locus_tag="HV256_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLU07912.1"
                     /translation="MLLLEKLNELDLDVEADLCEKLHDDAEHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            624..1025
                     /locus_tag="HV256_28560"
     CDS             624..1025
                     /locus_tag="HV256_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QLU07908.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADHSEQVISHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1774..2319)
                     /locus_tag="HV256_28565"
     CDS             complement(1774..2319)
                     /locus_tag="HV256_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU07909.1"
                     /translation="MPLPERMKPARVTRKQMKELISQLNGILDHIDNGMDENNEELKQM
                     MADWNAKVVMPCGFSDFRDFSSWTSALDFTRMALNQEKYLDDFTWTELNDVINFIRKAE
                     GSESDHSYALQLLEINFKANPLDLIYHPDCWFNDEALFHARLTTEEIGGYLMKKSGRWL
                     NDAPDIQLVYAIPLDVYD"
                     /name="hypothetical protein"
     gene            2687..5017
                     /locus_tag="HV256_28570"
     CDS             2687..5017
                     /locus_tag="HV256_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="klebicin B-related nuclease bacteriocin"
                     /protein_id="QLU07913.1"
                     /translation="MMGGFNYNGEGATSSGLSSPSASDVANQFNSFGGTQIRASDVSNI
                     RSDGNGGYMADIRGQIHTVSSEEGSTTSTTVGGFDGVRSSSTGNGGSQGGQGPQGQSLV
                     MHNGQMGYWENRSSGAGNNEHTTRAFVAVGPSEAEKAARAEKVAQEKQQAEEAAKAFAA
                     KTAAASAAAEKERQNAISAAAAAGQHQTVPDARNNLNQATAEASRLKTVADNALNTAKN
                     KRKEAIDAVPVATQAEKKYQDLQQSIKGLTLNNNGQYGTQKWEVISSNKEHDHWGYRFY
                     PSGITKAQVDAAQNDAVNKRNAATSLASQATAAEQASLQASAAYNAAETRRQAAQAALA
                     SAEQAAAAERKRHEAEAAAAAAAEKKRQADAAAKAAEEARAIAEKAKALQARRTAADKL
                     KSSEIQAVRGIPATAAPFAIPLTWSTASRGSFTLAADAAASLGAFISEALATLRVAVVA
                     NPVALTIAGLVLSKSVGVGSDMVPGRDISSMMPGDAFGLPDTAALNKAADQKTSVSMPV
                     RGRLVMNDSGILDVQLVKTNTAGAVKVARAVLDKETGYWGYTLPAVADVPAQTIFVSPA
                     DAPGANGPLTLSGPVPLPERILHTGDQISAPQATDKTVTPVADDLDFDDIILVFPPESG
                     LKPLYVMYRSPRNMPGTVSGKGQNVGNNWMGGASTGDGAPVPSQIADKLRGKTFGSFDS
                     FRRAFWKAVADDSALSKQFSEADINQMKAGRAPTADFLESVGKRVKIELHHEKEISQGG
                     AVMDVDNIKALTPKNHIETHKGK"
                     /name="klebicin B-related nuclease bacteriocin"
     gene            5019..5280
                     /locus_tag="HV256_28575"
                     /pseudo=""
     CDS             5019..5280
                     /locus_tag="HV256_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /name="bacteriocin immunity protein"
     gene            5384..5539
                     /locus_tag="HV256_28580"
     CDS             5384..5539
                     /locus_tag="HV256_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QLU07910.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            6273..6347
                     /locus_tag="HV256_28585"
     CDS             6273..6347
                     /locus_tag="HV256_28585"
                     /inference="COORDINATES: protein motif:HMM:NF013937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLU07914.1"
                     /translation="MTKQQKTALNMAKFIQNQSGVGSG"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6363..9260)
                     /locus_tag="HV256_28590"
     CDS             complement(6363..9260)
                     /locus_tag="HV256_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="QLU07911.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     Typing          complement(5745..6152)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.161"
                     /coverage="99.0"
                     /evalue="5.8799999999999995e-176"
ORIGIN
        1 cgagcgaaag aaagtcctgg cgcttgccca gtccagacaa attgatgcaa tcctggtcac
       61 tgagctttcc cggtgggggc gctcgacgct cgatctgctc aatacgctac gtgaactgga
      121 gaactggaag gtttccgtga tagccatgaa tggaatggcg ttcgatcttt cgtcgccgta
      181 tggacgaatg ctggcgacgt ttctttccgg cattgcggag tttgagcggg atctcatcag
      241 cgagcgggtc aagtcaggcc ttgctgttgc gaaggcacgt ggtaagaggc ttggtcgtca
      301 ggccggagtg cgaccaaaat cagaccgact tttgcctaag gtggttgcga tgagggccga
      361 gggacgcagc tatcgctgga tcgcacgcga gctcggtatc agcaagaata ccgtcgctga
      421 catcgtgcaa cgacacagag ctaacgctta gggtgtcatt tcgccctcag ccggaaccga
      481 ccccagtctc tgctcctgct ggagaagctt aacgagcttg atctcgatgt agaggccgac
      541 ctttgtgaga aactccacga tgatgcggag catcttttcc gtacactgtc ctcgagactg
      601 gatgcattac aggatggtaa ctgatggcca gagtgtctat tagtgaggct gcccgactga
      661 cgggtaagag tagaacaaca ctgcacaggt taattaaagc aggtgacctg tccacctgta
      721 gcggtgaacg caatgcaaaa atggtagata cttcagagct tttacgtgtc tttggtccct
      781 ttgaacaacc gaagggtgaa caccattctg gacaggtctc tgaacagcgt gatacagcct
      841 ctgctgacca tagtgaacag gtgattagtc atctgaaaca ggagattgaa cacctgaata
      901 ccttactttt gtccaaggat tcgcacattg acagcctgaa acaggccatg ctccttcttg
      961 aaaatcacca ggcaaaatta actgaatccc cggcgccatg gtggaaattc tggaaaaaat
     1021 cctgaccccg tcatgcttcc gttttcctct ccggccatcc cggggagttc ctgaaccttc
     1081 ccaaaaatcg ccagatctct gaacgctcct cacgactaaa aatacccaca cactacgcaa
     1141 aattaaaatt ttgcagataa ctccttaata tcagttagtt agatgttatt aaattacgct
     1201 gtattacatt ctgtaaggta aatccaagtc gtcggcgatt cgcgtacgac ttgtcgatga
     1261 ctcgtcatag agtcatcgac gactcactta agactatcag taacttatga cgacttgact
     1321 ctagagtcga gtcgtcattt tggttttttt ttgaccttgg cgactctaga gtcaagtcac
     1381 gagtcgaatc gccggtgaat cgtgtggtga gtcgtccggt gacttgccat aacgtgattg
     1441 ccggccgtcg acgaacccga ttcctgaacg tgatgaaaac cggttttccg gctgcgccgg
     1501 caacgcttca cggccacgca ataaattgcg tccccgttca ccgttttcgg ataggaagaa
     1561 cgccggaaac aggacttacc ctggctgaat ggtgtgaatt ttcgcgctat gcacttgcgt
     1621 gcatactcat gcatgccgta aaaacagagc ctgcgcgttt ctggcgggtt ttcgggtggt
     1681 ttgttgcctg ttttgccggt ttcccgtcgg aaacgccctg aggccgtttt agcggtgcgt
     1741 acaattaagg gattatggta aatggtgact tggttaatca taaacatcca gggggatggc
     1801 atacaccagt tggatatccg gtgcatcgtt gagccatcga cctgattttt tcattaagta
     1861 gccaccaatt tcttcggtag tcagccgggc atgaaacaat gcttcatcat tgaaccagca
     1921 gtcagggtga tagatgagat ccagtgggtt tgccttgaaa ttaatctcca gtaactggag
     1981 tgcatagctg tggtcagatt cactaccttc tgctttgcgt atgaagttga taacgtcgtt
     2041 cagttctgtc catgtaaagt catcgaggta tttttcctga ttaagcgcca tcctggtgaa
     2101 atctagtgca cttgtccagg atgaaaaatc acgaaaatca gagaacccgc agggcatgac
     2161 gacctttgca ttccagtctg ccatcatctg ttttagttct tcattgtttt cgtccatccc
     2221 gttatcgatg tgatcgagaa tgccattcag ctgactgatc agttctttca tctgtttacg
     2281 ggtgacccgt gcgggtttca ttcgctcagg taatggcatc agaaatactc tcttttttat
     2341 cgtgcactcg ggggaactga gtaatctatc gggctatgaa tcagagcaca aatcgttttt
     2401 ttttctttct gatgatccgc attctgcctc agatttacct ctgtcgtaac ttttaaccga
     2461 ttgaaaataa ataaaaatat tgttgacgtc tgcatattga cagagctggc atgtcaagag
     2521 tagtatcaaa ctactgtata taaacacatg tttttatata cagtagtttg gcgtggcaga
     2581 gaactaataa cagcctccgg ataactgtcc tgaagcaata aaccgcacgc cggatttcag
     2641 aaaagtggaa aacaaaaaaa acaaaaaaaa caaaagaagg aaatgtgtga tgggtggatt
     2701 taattataac ggtgagggtg ctacgagctc aggccttagc tctccttctg ccagtgatgt
     2761 ggctaatcag tttaatagtt ttggtgggac gcaaatccgg gcatcagatg tctcgaatat
     2821 tcgcagtgat ggtaatggtg ggtatatggc ggatattcgg gggcagattc acactgtgtc
     2881 ctctgaagaa ggctcaacaa cttcaacaac tgtcggtggc ttcgatggtg tccgcagttc
     2941 ttctaccggt aatggcggaa gtcagggtgg gcaggggcca cagggacagt ctctcgttat
     3001 gcataacggg cagatgggat actgggaaaa ccgatcatcc ggggctggta ataacgaaca
     3061 tactacccgc gcttttgtgg cagtaggccc atcagaagca gaaaaagctg cacgagcgga
     3121 gaaggtagct caggaaaaac agcaagcaga agaggctgca aaagcattcg cagcgaaaac
     3181 agcagcggca tccgccgctg ctgaaaaaga acgtcagaat gcgatttctg ctgcggctgc
     3241 tgcaggtcaa catcaaactg ttcctgatgc gcgtaataat ctgaatcagg cgactgctga
     3301 agcatcacgc ctgaaaacag tggcagataa tgcccttaat acagcgaaga ataagcgaaa
     3361 ggaagctatc gatgccgtac cagtagcaac tcaggctgag aaaaaatatc aggatctgca
     3421 gcaaagcatt aaaggcctca ctctaaataa taacggccaa tacggtaccc aaaaatggga
     3481 ggttatcagt tcgaataaag agcatgacca ctggggatac cggttctatc catctgggat
     3541 cacaaaagct caggttgatg cagcgcagaa tgatgcggtg aacaaacgaa atgctgctac
     3601 cagtcttgcc agtcaggcaa cggccgctga gcaggctagt ctgcaggcat cagctgccta
     3661 taatgctgca gaaacgcgcc gtcaggctgc ccaggctgca ttagcgtccg cagaacaagc
     3721 cgccgctgct gaacgtaaac gccatgaggc ggaagcagca gccgccgctg cggcagaaaa
     3781 aaaacgacag gctgatgcag cagcgaaagc cgctgaggag gccagagcca ttgctgaaaa
     3841 agcaaaagcg ctgcaggccc gtcgtacagc cgcagataaa ctgaaatctt cagagattca
     3901 ggctgtcagg ggtatcccgg ctacagctgc accttttgct attcctctga cctggtcaac
     3961 tgcgtcacgt ggaagtttta ctctggcggc tgatgctgcg gccagcctgg gagctttcat
     4021 cagcgaggct ctggcgacgt tacgtgtcgc tgtggtggct aacccggtag cattaaccat
     4081 tgccggtctt gtgttatcga aatcagttgg cgtcggtagt gatatggtgc ccggtcggga
     4141 tatcagttcg atgatgcctg gcgatgcgtt tggtctgcct gataccgcgg cattgaataa
     4201 agcagcagat cagaaaacat ctgtcagtat gccggttcgt ggccgtctgg ttatgaatga
     4261 tagcggcatt ctggatgttc agctggtcaa aaccaatact gccggtgccg tgaaggtcgc
     4321 tcgtgctgta ctggataagg aaaccggcta ctggggctat actcttcccg ctgtcgcaga
     4381 tgttccggcg caaactattt ttgtcagccc ggctgacgca cctggtgcaa acggaccact
     4441 gacgctaagc gggccggtac cactaccgga gcgtattctg cataccggtg atcagatttc
     4501 ggcgccacag gctacagata aaaccgtgac gccggtagcg gatgacctcg attttgacga
     4561 tattattctg gtgtttcctc cggaatccgg cctaaaaccg ctgtatgtga tgtaccgtag
     4621 ccctcgtaac atgcccggta cagtgagcgg caaagggcag aacgttggca ataactggat
     4681 ggggggggcc agtaccgggg atggtgctcc tgttccttcc cagattgcgg ataaattacg
     4741 cgggaagact ttcggtagtt ttgattcttt ccgtcgggcg ttctggaaag cggttgctga
     4801 cgattctgca ctgagcaagc aatttagtga agctgatatt aatcagatga aagctggaag
     4861 ggcacctact gcggactttt tagaatcagt agggaaacgt gttaaaattg aattacacca
     4921 cgaaaaagaa atatctcaag gaggggctgt tatggatgtc gataacatta aggcgctaac
     4981 acctaaaaat catattgaga cacataaagg aaaataaaat ggctggtaat aaaatttcag
     5041 attttacgga agaagaattt actgcttttg ttagtaaaat aatcgctgct gattttccaa
     5101 ctgacaaaga gaacgatgat gctatttatt cgttcgccca actaacagag catcctgctg
     5161 gttggaacct catcttcaag cctaagatag gcgaagattc ttcggcgaaa gggattgtta
     5221 aaaccgtaaa ggaatggcga gctgctaacg gtaagccagg ttttaaaaaa gcgtgattga
     5281 atttgcgggt tcaagttatg agcccgctca tttgtaactc tggatttgaa gaataattac
     5341 tagatatcca tttgcctaga ataataaaaa taaaaggtga tatatgaaaa aattactttt
     5401 cctaggggtt gttaccgttt ctgtggttct ggcagcatgt caggtcaata atgttcgaga
     5461 caccggaggt ggggctgttt caccttcatc gacggtgacc ggagtcacgc ttgatagcgg
     5521 tacgaacggc aatccgtaaa ggatcttctt gagatcccat ttggatcgtc gtaatctctt
     5581 gctctgtaaa cgaaaaaacc gccttggcgg gcggtttttt cgaaggttcg aggagttggc
     5641 gctctttgaa ccgaggtaac tggcttagag gagcgcagta accaaattcg ttctttcagt
     5701 ttagccttaa ctggcacata acttcaagac taactcctct aaatcagtta ccagtggctg
     5761 ctgccagtgg cgcttttgca tgcctttccg ggttggactc aagatgacag ttaccggata
     5821 aggcgcagca gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     5881 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg
     5941 acaccggtaa accgaatggc aggaacagga gagcgcacga gggagccatc agggggaaac
     6001 gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcaaattctg
     6061 tgatgcttgt caggggggcg gagcctatgg aaaaacggcc gctgggcggc ctcttctttt
     6121 ccgcctccct tgatcgctcg gttttctcga gcttttataa gaacggtctt gccgctcgcc
     6181 gcagccgaac gaccggagcg tagcgactga gtgagcgagg aagcggaaaa gagattgttc
     6241 ttacactgag cactgacgct ctgaggcttc ttatgacgaa acaacagaaa acagcgctga
     6301 acatggctaa attcatccag aatcagtctg gggtcggttc cggctgaggg cgaaatgaca
     6361 ccctaagctt tcggttcctt gggccaaaga tattcgccag tcagtagaat gtgcgcccag
     6421 cccaatgggg atatgtgggg aagaaattca gggggaacat ccaacccttc gttccgccgc
     6481 tccgtgacgg catgaccaag atggacggta ttccagtaaa tgatcaccgc agtcaataaa
     6541 ttgagcccag cgattcggta gtgctgcccc tctgtcgtgc gatcgcgaat ttccccctgc
     6601 ctcccgatac ggagcgcatt tttgagcgca tggtgggcct ctcccttgtt aagaccgatc
     6661 tgagcacgcc gctgcatgtc cgtatccagg atccactcaa taatgaaaag ggtccgttca
     6721 atacgaccaa cttcacgaag cgcaactgca aggttgtttt gtcgtgggta agaagcgagc
     6781 ttgcgcagga gttggctggg cctgattttg ccagcggtca tcgtcgcggc acaacggaaa
     6841 atatcaggcc agttcgcaac gataagatcc tcccgggctt ttccacctac caacttgcgt
     6901 aactccctgg gggtcgtatc gggattaaat acgtacaacc gcttcgatgg cagatccctg
     6961 attcgcagaa cgagattgta gccgagcagg ctactggctc cgaacaaatg gtcggtgaat
     7021 cctgctgtat cggcatactg ttcgcgaaca tggcgaccga cctcgttcat cagtagtcca
     7081 tcgagaatat acggtgcctc gctcacggtc gccgggatcg actgacaagc gaatggcgcg
     7141 aactggtcgc ttacgtgagt atacgctttg aggccgggaa cagaaccata tttggcattg
     7201 accatgttca tggcttcgcc atgccgcgct gtcgggaaaa actgaccatc gctcgatgct
     7261 gacgtgccca tcccccagac gcgtgacatc ggcagtttac cctgcgcggc caccacaatt
     7321 gccaatgcct ggttcatggc ttcgctttca acatgccagc gggcaaggcg tgagagctgc
     7381 cagtaatcat gcgtgtttgt agcttccgcc atcttacgca ggcccagatt gagcccttca
     7441 gcgagcagga cgttgagcag accgatccgg tcgcgacatg gagccccggt tctcagatgg
     7501 gtaaacgcat ctgtgaaacc aagggctgca tcaacttcaa gcagcatgtc ggtaatccga
     7561 acggacggca ttcggcgata cagatccagt atgagtgcct cggcaccatc cggcacgtct
     7621 gctgtcaacc tgtcgatccg caacgttcca tcttctatgc taccgtgcgg aatagtgccg
     7681 ttacgggcag cccgggccag ccgcttaaga gcgatcgtga gtcgcgcctt tctgtctgcc
     7741 agccaatcct gtgggttgga aggcacggcc agttttgcat tttcctgcgc cgcgatcatc
     7801 ggcaccagta cctgcttgag gtcaccatag cggcgcgaat gagcgagcca gacatctccg
     7861 gaacgaaaag catcccggag gtgaaagagt accgccactt cccaaagacg ggtatctcct
     7921 ttttcctgag ctcgtaaatg acggttccat ttggagctgg gccgcaggaa acgcctttct
     7981 ggcgatgcaa cacctttcat ctctccgatc gacaaagctg ctgctaccaa tggtccggcg
     8041 accggcgcgg cttcgagctt cagacagcgc aacatgcggg gcgcataacg acgaaagcga
     8101 tggtatccct gcccgacata tgcaagaggc tcatcggcta gcgtgttgct gagttgagtc
     8161 cctgtcgcta ccagttgagc gagccggtcc catgcaaccg aactggcgac agccatctcc
     8221 agcggggttc cgtcactgcg ggcctcaagc aacgaagctc ccagcgcggt gaaggtacgg
     8281 atcgtatccg tgagtgtggc tttagagccg gaaattgttt cgtcatgctg gcgcttcgct
     8341 tcccgccagg tttttcctac gatcctgtca tgggtttcga ctatggcatc agcaatcgcc
     8401 gcttcccact ccacaacaca gacggcaagg atcgcccagc ggcggtccga agtgatgtca
     8461 cgcaaaccgt cggtgaagta gcgttcaccc tgccgacgca gccgggcaat gcgatgggca
     8521 ggtatgctgg ccaaagcact atgattgata ttcagggtac gcagaaattc gagcctgtcg
     8581 agcaaacggt tagcagcagc cgagttgtta ccaacctcga agttgcgaag ccagatgaaa
     8641 cgactgatat tgccggcgag catttcactc agaagtttgt ccaggtgatc gcgaacatcc
     8701 gctgttaaat tttccgcaat ccgcgtttca atccgccgct cagcggcgac cagagcatcc
     8761 gcgcacaagc gctcgattgt cgatactgcg ggcagaatgg tggaagtttc ccgacaccgc
     8821 acaataaaac gatgagcaag atcctcgttt gatctggcat cttcggcctg gccgaaagtc
     8881 cactcccgca gatcacgggc accacggccc gtgaaggtct tgtagccgta aatttcgcgc
     8941 agcgtgtcca tgtgctgctg acgggtttgg cgccgtgtgg cataagtgag aagcgcatca
     9001 gccggaactc caagctgagc accgacgaag gaaaggactt cacgcgggat catctcacca
     9061 ggagccagtg cacggcccgg atatcgtaag gcacaaagtt gcagggcaaa gccaatcctg
     9121 ttttccggtc tgcggcgctg cctaatgttt tccaggtcat catcgcccag cgtgtagaac
     9181 ttcagtagcg acagttcgtc cgtgggcaga tcgaacagcg ctgctcgctg ccgttcggtg
     9241 aaaatatggc gtcgtgacat acaaattcgt cccttttgaa gtatagtctg ttttggacaa
     9301 cagccagccc atataaatca gggcgttccg atacaaaaat ccaggagggt tcaattggga
     9361 catcgtgccg ccatttactg ccgggtttca acagcggatc agtcttgtga acgccaggaa
     9421 tttgatctgc gagccttcgc cggccgtgcc ggctacgacg tggtgggaat atttaaggaa
     9481 acaggttcag gaactaaact cgaccgggc
//
