LOCUS       CP056661                2865 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00204 plasmid pRHBSTW-00204_7,
            complete sequence.
ACCESSION   CP056661
VERSION     CP056661.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148538
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T1-00204
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 111.15921851906x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 00:19:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,735
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,439
            CDSs (with protein)               :: 4,439
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 63 of 178
            Pseudo Genes (incomplete)         :: 91 of 178
            Pseudo Genes (internal stop)      :: 42 of 178
            Pseudo Genes (multiple problems)  :: 17 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00204"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00204_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(2529..2865,1..635)
                     /locus_tag="HV121_23670"
                     /pseudo=""
     CDS             join(2529..2865,1..635)
                     /locus_tag="HV121_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427687.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            931..2097
                     /locus_tag="HV121_23675"
     CDS             931..2097
                     /locus_tag="HV121_23675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU12373.1"
                     /translation="MSYQFIHIEDYGINISKKRSNNGTSEKYKKETKGRSVSQIISEAK
                     RDNGFCSHVEKPEDPIILYGKSLDEVEKLAMEYHSKTKIIDKNGKEKKLRSDANVLLAG
                     VISLNRENMEIWEDYKNDSIAYLKNKYGKKLISVIEHTDEEHPHIHFYCIQDIGKKFDL
                     IHDGKKALHENKDKKKHDQNIAYLDSMRAFQEDFFKVVSSNYGLSKDGPRRARMSRSDY
                     FKLGREVKLINQVKLKAKKDGYKFAIDEFESKNWLNKITASITFNKDSLNEAISRGNKY
                     KKSLSSKTKKLKELEDIETKYNDLRKDFKLRVNNQTENLRNKNNTLEYENENIKNKNKE
                     LEEENKKLKTTLEETSPLYELLKNKFGDQFEEWKNNLFKSFRSKIKPK"
                     /name="hypothetical protein"
ORIGIN
        1 aatataaatc tgcaaggtgg atgtttctaa ctttaacagt tgaaaactgt gagataactg
       61 atttaaaaag caccctacaa tggatgaata aatcatttaa aaaactatgt cttagaaaac
      121 agtttttaaa aaataatatt ggtttcgtaa aaaccaccga aataacaaga tcgaaagatg
      181 gaaaagctca ccctcatttc catatattgc taatgatgaa gagtacttat tttaaaggta
      241 ctgaatatat gaaaaccagt gattgggctg agttgtggag agactgcctt ggagtctctt
      301 acacacctat aacagacatt aggactgtta aggtcaagaa cggcggggat tccagccgtt
      361 cggctcttgc tgatgctgtg gctgaaacct tgaagtatgc tgttaaacct tcggatatga
      421 caaaagatcg tgagtggttc cacgaactaa ccagacaagt ttttaaatta aggtttgtgt
      481 ctactggtgg agtattgaaa gacctattta aggaagaaac ttccgaagaa gatttattat
      541 tgcttaatga agaagaggaa acagatcaag acttgggatc tttaactttt aactggcata
      601 aaacaaaatc taaatacaga agaaaaataa cagaatgata aatagtagtg attataaaaa
      661 ataatataag gagaatatta tgatcactgc tataactgta atatctattt tatcaaacat
      721 actaacgatt atagtttgta taaaaacact aatcgggaag taataaaaaa ggcacctgta
      781 agggtgcttt ttttatgcct ttgattttag cctccggcag gaccgcgttt attcgaagaa
      841 taaacatagt cggttcctat gtatttacaa aaacggggaa tatgctaaaa aatatatata
      901 ggaaaaaata taaaagcagg ggtaaatata atgtcatatc agtttataca tattgaagat
      961 tacgggataa atatatctaa aaaaaggagt aataacggaa cgagtgaaaa atacaaaaaa
     1021 gaaacaaaag gaagatccgt tagtcaaata atatcagaag caaaaagaga caatgggttt
     1081 tgttctcatg ttgaaaagcc agaagatccg attattttat atggtaagag tttagatgag
     1141 gttgaaaaac tcgctatgga ataccatagt aaaacaaaga taatagataa aaacgggaaa
     1201 gagaaaaaat taagatctga tgctaatgtt ttactcgctg gggttatttc tttaaatcgt
     1261 gaaaatatgg aaatatggga agattataaa aatgactcga tagcttattt gaaaaataag
     1321 tatggtaaaa agcttatatc agttattgaa cacactgacg aggaacatcc tcacatacat
     1381 ttttattgta ttcaggatat aggtaaaaag tttgatttga tacacgacgg aaaaaaagca
     1441 ttgcatgaaa ataaagataa gaaaaaacac gatcaaaata tagcttactt ggattcaatg
     1501 agagcctttc aagaggattt tttcaaagtt gtaagctcta actatggctt gtcgaaagac
     1561 ggccccagaa gggctagaat gagtcgttct gactacttca agcttggtag agaagtaaag
     1621 ttgattaatc aagtaaagtt aaaagcaaaa aaagatggtt ataaatttgc aatagacgaa
     1681 ttcgaaagta agaactggtt aaataaaata acagcaagta ttacttttaa taaagactca
     1741 ttaaatgaag ctatttctcg tggtaataaa tacaaaaaat cgcttagtag taaaactaaa
     1801 aaactaaaag aacttgaaga tatagaaact aaatataatg atctgagaaa ggattttaaa
     1861 cttagggtta ataatcaaac tgaaaaccta agaaataaaa ataacactct tgaatatgaa
     1921 aatgaaaata taaagaacaa aaacaaagaa cttgaggaag aaaataaaaa actaaaaacc
     1981 actcttgaag aaacttcccc actctacgag ctattaaaaa ataagtttgg ggatcagttt
     2041 gaggaatgga aaaataactt gtttaaatct tttagatcca aaataaaacc taaatagtta
     2101 aatagtcaat aagaatggcg gggggtcccc tctggggttc cgctattgtt gttgactaca
     2161 cacccaccaa tacacttcca atggtgtaag gagagcaaag cgacccttac acctgttaag
     2221 ccggttttaa gctcttagaa gccctctgga agcctcagaa ggcttctggc ttgtggtttg
     2281 gtgggtttgt tccagtggtg gttttgggtg ttaaaaacag cctcgcagag ggtgattttc
     2341 ctttacttga taccttaggc ataacggcag gatagaagga taactgtaaa ataggctcta
     2401 agccttgtcc cgtaagggct tggagtgttt ggtgaggtgc ttttttggta taacgttgac
     2461 aaaaaaaatc cccgtgttaa gttttgagtg tcgaagtcaa aaactataat acggggatcg
     2521 gtatatctat ggagagaata gataataaaa aaataaaagt caagtttgaa aatttagcag
     2581 atagatcgga aaaagataaa gtctgggata ttcacaggaa agactgtgat ttagtttctg
     2641 cattatattt tcagtcagca gagttcgaga agtatttcga acgaatggaa gattgttcag
     2701 gcaggttaga gtttaatcag cttctggata aggaaacagg agagataaag attaaactaa
     2761 caaaaaccca cttttgccgc gttcgtcatt gtcctgtctg tcagtggagg aaaagcttaa
     2821 tgtggaaagc aaaattttat aaagcactgc ctgagatcga agcgg
//
