LOCUS       CP056660                3675 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00204 plasmid pRHBSTW-00204_6,
            complete sequence.
ACCESSION   CP056660
VERSION     CP056660.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148538
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP3 T1-00204
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 111.15921851906x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 00:19:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,735
            CDSs (total)                      :: 4,617
            Genes (coding)                    :: 4,439
            CDSs (with protein)               :: 4,439
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 63 of 178
            Pseudo Genes (incomplete)         :: 91 of 178
            Pseudo Genes (internal stop)      :: 42 of 178
            Pseudo Genes (multiple problems)  :: 17 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00204"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00204_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2858..3675,1..304))
                     /locus_tag="HV121_23655"
     CDS             complement(join(2858..3675,1..304))
                     /locus_tag="HV121_23655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU12372.1"
                     /translation="MDFKIKKMTVKDSREIKKKFEDCIKIAEEKNMQIGSIINIEMQDR
                     LKEINLKVKKETAEQYRKLFNYKLSVNAEDIIQHLLECNTRNSFDKTRTAFRFCIAEKI
                     QELRNEADRARKQKNFDLMKEKTIEAYKLFFFFERDFLSENRVTWNDISYRKIDSKSKK
                     KTMNSVSSIKAIFEDLKQKQELFDKYSMILAISSLTGCRPAEIQKGISLEVSPPLLNIC
                     ISGAKVGEDRGQEKRILKFNINEFAENEQFKLIMSKIKQNNKLEYKCSKQEYDSLRQYL
                     YINHKGFSLYTLRHRAASELKKDGFSEEMIAAFLGHRVTRSLENYGYARSGKGGPKVAG
                     VEHSNAIKSNTKRYKSKTTPDGGNPLPTRKRAL"
                     /name="hypothetical protein"
     gene            complement(678..1379)
                     /locus_tag="HV121_23660"
     CDS             complement(678..1379)
                     /locus_tag="HV121_23660"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU12370.1"
                     /translation="MTISKKNSELRERFKEYSSNKNIFDLADLRYEILKIYFDFKLKND
                     MNEQERKSQDSRRKAHLTALKKRIKREIVSKIVIDLVKYYNIEKTTFHFFSHICTEILE
                     RNVDNRYILNNFSNMILDEKKELTKLSESRNASNKMSLENSYSELVSMSHIKDKLFRND
                     NFKTAYLKCYGCANEEFSRFKVFAFPDNFETLDFLFEEERIKKEEKEISKIMIEQVEEE
                     PKIQPIKKRRL"
                     /name="hypothetical protein"
     gene            complement(1445..1693)
                     /locus_tag="HV121_23665"
     CDS             complement(1445..1693)
                     /locus_tag="HV121_23665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU12371.1"
                     /translation="MEIEKLIDRYFLALERKVIREIYDKQTREELEKFIFKCNGEFREF
                     ERRFNTICSTEYYEQQSLIFYINYINAEFEQREKLNY"
                     /name="hypothetical protein"
ORIGIN
        1 cagcaatgca aaatctaaaa gcagttcttg ttttatcaaa actgtttctt gtgttgcatt
       61 ctaacaagtg ctgaataata tcttctgcat tcacagacaa tttataatta aacagctttc
      121 tatattgttc tgctgtttct ttttttactt ttaaatttat ttctttcaat ctatcttgca
      181 tttcaatatt tataattgaa ccgatttgca tatttttttc ttctgctatc ttaatgcaat
      241 cttcaaattt ttttttaatt tctctgctgt ctttaacagt catttttttt attttaaaat
      301 ccataagcca cctcatttat ttatagtttt attgttttaa ttttactccg gtaggtttgc
      361 cttttttctt cattcctaat aaattgtttt tcaaaatcgc ttcgctctaa tgaaaaaaaa
      421 tttaaaagtc attccaaaaa aaagcaaaag ggttttgtgc tcttcgcaca cttttccttt
      481 caaatttcgc tgacgctcat ttttcaagta aaagacaacc ccccattatt gttataacaa
      541 aaataacaat aatggcaagt caaaagaaaa aatagatcgc agattttact agatttttca
      601 tcaacttaca tacagatcaa tcgctcaaaa aacaaaatta aaattttatt tttctcacga
      661 ttcgccgcaa aaaaaaatta taatcttctt ttctttattg gctgtatttt tggttcttct
      721 tcaacttgtt caatcattat tttagatatc tctttttctt ctttttttat tctttcttct
      781 tcaaataaaa aatctaatgt ctcaaaattg tctggaaaag caaaaacttt aaatcttgaa
      841 aattcctcat ttgcacaacc ataacatttt aaatatgcag ttttgaaatt gtcattccta
      901 aataatttgt ctttaatatg tgacatagaa acaagttcgg aataagagtt ttctaaactc
      961 attttgtttg aagcatttct gctttcactt aatttcgtta actctttttt ttcgtctaaa
     1021 atcatatttg aaaaattatt tagaatgtat ctgttatcta catttctttc taaaatctca
     1081 gtacatatat gagaaaaaaa atgaaaagtc gttttttcta tgttgtagta ttttactaaa
     1141 tcaataacta ttttcgaaac gatttctctt ttaattcttt tttttaatgc tgttaaatgt
     1201 gcttttcttc ttgagtcttg agattttctt tcttgctcat tcatatcgtt ttttaattta
     1261 aaatcaaaat atatttttaa tatttcatat ctcaaatcag caagatcaaa tatgttttta
     1321 ttacttgaat actctttaaa tcgctctctc aattcgctat ttttcttaga tattgtcata
     1381 tattaagccc tctgtgtttc gctaatacat atatgcttaa tatattattt taatgttgtc
     1441 aaatttaata atttaacttc tccctctgct caaactctgc atttatataa ttaatataaa
     1501 agataaggct ttgttgttca taatattcag tagagcatat tgtattaaat cgcctttcaa
     1561 actctctaaa ctcaccatta cacttaaaaa taaatttttc taattcttct cgtgtctgtt
     1621 tatcataaat ttcacggatc acttttcttt ctaatgctaa aaaatatcta tcaatcaatt
     1681 tttctatttc cataagtttt ccttttttta tatgttattt ggaatttaaa tagcaaattg
     1741 caaaaaaaaa tcaaatttcg aatttttttc ttcgaactct atttttttta ttctttagta
     1801 ttgtttttct atatttctac agatcagcat tgctttttca ctataacaga tctgtattgt
     1861 tttactgtgg cttcgctaag acctttgggt tcttcccttt cagggcttca cccaaaggtc
     1921 tcccctagta aaaattttct ggatctaccc taaaaatcag gtaattagaa aattttcact
     1981 aagggcgatt tgtgtgattg caaagtttta gacttcacca caaagttcta gactgtcgag
     2041 ctgtgcattg tttccaacac acggcacccc ctcacaccct ctagatacag ttcatagctt
     2101 ttacttgtga cagttaaccc catttttaac accttactgg ctgttatggc tgggctttct
     2161 catagcctac caatacaaac actgttcacc aaaatggcct tagaactcag cctccaggtt
     2221 cggcaatttg tgagatcccc ttctattgcc aacaaccaac cgaaaccgga ccctcttgat
     2281 tccacagttt caggcacgac acaatatgcg catttggttg cttaaagact tgaaaaagat
     2341 ggattttttt tgagtgttgc aatgaagtga agtttgactt ttacgaaaat gtgagttaaa
     2401 ttcttactta catatcgtaa tcgagtgggt gagatcactt gattacaaga cttttagaag
     2461 ttccaccttc ttagagtccc tcaaaaccag ccaaaagctg gttttgttgt atctggactc
     2521 agttataatc tatctgtctg ttctgtcaat agttcttcaa aaaatttgat gcctcaaaaa
     2581 tcaaaattct cagtcggtga gactcgttgt gtcagttttg ctgttaaaaa tgacacttca
     2641 accgccaccg gctgacagtt aattgttcaa aaaacaaaca atgatacatt cccctggttc
     2701 ccgttccggg cattatgcgc gcgtgccgcc tgctaataca tatattgatt agttaattac
     2761 accgaccaca caatctgtgc cggcacgaag cgcgcgcgag gctaacacac atatgaggga
     2821 tcagcattgc tgacccctgg taactctcta aaatcgtcta caaggctcgt ttacgagttg
     2881 gtaaagggtt tcccccgtct ggggtggttt tgctcttata tcgctttgta ttgcttttaa
     2941 tcgcgtttga gtgctcaaca ccggcaactt ttggaccacc tttcccggat ctggcatatc
     3001 cgtaattttc taaactcctg gttaccctgt gacccaggaa agctgcgatc atttcttctg
     3061 aaaaaccgtc ttttttcaat tcacttgctg ccctatgtct taatgtatat aaagaaaatc
     3121 ctttgtgatt aatatataaa tattgtctca atgaatcata ctcttgcttt gagcatttat
     3181 attctaattt attattttgc tttatttttg acataatcaa tttgaactgc tcattttcag
     3241 caaattcatt tatattgaat ttcaatattc ttttttcctg tcctcgatct tcgcccactt
     3301 tcgccccgct aatacatata tttaatagtg gggggcttac ttccaggcta atccctttct
     3361 ggatctcagc cggacggcaa cccgttaaac ttgaaattgc taaaatcatt gaatacttat
     3421 caaaaagctc ttgcttttgc ttcaaatctt caaaaattgc tttgatactt gaaacactat
     3481 tcatagtctt ttttttgctt ttagagtcta tctttctgta agaaatatca ttccaagtaa
     3541 cgcgattttc gctcaaaaaa tctcgctcaa aaaagaaaaa taatttatat gcttcaatag
     3601 ttttttcttt cattaaatca aaattctttt gctttcttgc tcgatctgct tcatttctta
     3661 gctcttgaat ttttt
//
