LOCUS       CP056634                4072 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00303 plasmid pRHBSTW-00303_3,
            complete sequence.
ACCESSION   CP056634
VERSION     CP056634.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148565
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00303
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 93.1772218741499x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 23:54:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,679
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,469
            CDSs (with protein)               :: 4,469
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 51 of 92
            Pseudo Genes (internal stop)      :: 12 of 92
            Pseudo Genes (multiple problems)  :: 10 of 92
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00303"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00303_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(487..741)
                     /locus_tag="HV148_23385"
     CDS             complement(487..741)
                     /locus_tag="HV148_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QLW81882.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     gene            920..3079
                     /locus_tag="HV148_23390"
     CDS             920..3079
                     /locus_tag="HV148_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QLW81883.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            complement(3335..4027)
                     /locus_tag="HV148_23395"
     CDS             complement(3335..4027)
                     /locus_tag="HV148_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLW81884.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(743..919)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          920..1278
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          complement(3336..4027)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          834..3076
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aataagaaat gtggaatatt tttttttata tcctcacatt tctgtgaata acaccttaaa
       61 ttcctccacc tttaccttaa attcctccac ctaaacacct taaattcctc cacctttacc
      121 ttaaattcct ccacatatac ctctataaca tattgatttt attaatgaat tttgacccta
      181 aaacaagtat taaaaacaag atcttaaaaa caagatctaa aaacttaacg gcgagcctgt
      241 ggataacttg gggcgctgaa aacaaaaaac caataaaaac aatgtgttgc aagaaagcag
      301 aattgcaagt tccgaatact ccccttccaa ccctgtggaa atccagtatt catgcgggtt
      361 cagcgcatat cacctaccga aaaaccgtgc gtatgtgctc aacctggcga cggattcact
      421 tccgcttcct cgcccactcg ctcgctaacg ctcggtcgct ggggctgcgg cgagcggacg
      481 atacaatcac tgtcctggca gcgcctccag ctcgttaatc agcggcagta tgcgcgtcac
      541 atcagcctgt cgtgtcacct ctgccttcag gaactggagt aaccaggcac gcaactgcac
      601 ctcctccggc tttctgacct ttgccagcaa tgctgcaccc acaacaacct tctgcgctgt
      661 gtccaggctc cggctcttcg ccttcaggcg ctgtaatctc gcctcagcat cagcaatctt
      721 ctgttcgagc gttctgctca tgatgtaact ccgtgcgcga tgaaaaatcg cattttagcg
      781 cgtcactggt agtttaaaaa ctgagctggc ataatgcgcg acaaatcaca aagtgcgcac
      841 ttatacaatc tccactccgt ttcgattgtg tgcgccctgc ggggctgaaa gaaaactaca
      901 aaaaggcatt acggcagaaa tggcgattta tcatctcagc atgaaaatta tctccagaag
      961 cagcggctac agcgccgtag cttccgctgc ctatcgttcc ggttcactga tgcttgatga
     1021 gcgtaccgga ctgactcacg actacacccg caaaagcggt gtggctgaag ctgtgattct
     1081 gacccccgcg acagcccctg cctggtgtac gaaccgtgcc gaactctgga atgccgttga
     1141 gaaggccgaa tgccggaaga actcacaact tgcgcgggaa attgaactcg ctatcccccg
     1201 tgaactgccg caggatgccg cacgggaaac cgttctcgcc tttgtcagag aaaactttgt
     1261 cagtcagggc atgattgctg atgtggcgtt ccatcacatg gataaaacaa atccccatgc
     1321 gcacatcatg ctgaccacga gggctgtcgg acctgctggg ttcggcggaa aggtcaggga
     1381 ctggaacgac cggacacatg ccgagacgtg gcgtgcatca tgggctgacc atgcaaacag
     1441 agcactggcg aacgccggtt atcaggaaga gatagaccat cgttcatacg agcgacaggg
     1501 gcttgagaaa acgcctggta ttcacctcgg aaagtctgcc tgtgcgatgg aaacacgcgg
     1561 tatcgaaaca gaacgcggcg aacagaatcg tctgattaac aggcttaacc tggaaataca
     1621 gatttcccgc actgaactca ggaacagcgg gctgacagag cctgcccgcc gtgtggctga
     1681 ccttctgaat atcgtgcttc ctgacaacgc aacttcgtac acactacgtg acatcatcga
     1741 agcgttgccg aaggacagta atgcggcatg gaaactcacc agtaatgcca tgtcgatgat
     1801 ggcagatatg caggcgacgc gcagacgctg ggaagagctg aacaccgaac gaaaggaagc
     1861 gatgagccat gctgccagcc tgaaaaaatc atcgccgttt tcgtccggtt tttcccgtat
     1921 tcccctgatg caatgggcgg caccggaata ccgtagcgaa cagggaaaaa tccagtcgct
     1981 gagccagcaa atggaaaaat tgcgacagca ttaccggaag gtggagaaac aggacattcc
     2041 cgcgagtcag acggcatttg aaaaacagtg gcagcagtgg ggggcatccg gccttgcagc
     2101 tctcagagtc cagttgctta aacgtgaaga ggaattgcgc cggaaggagc aggaggatac
     2161 agaacgtagt cgcgcggagc gacgagcgga acagctcagg aaaaatgaca atgccgttat
     2221 ggattcaggg gtactgagcg cagtcgttac ggggtatggc gaggccccca tgcctggtaa
     2281 cggggaaatg acctgttacc ttctgcttca caaccggaac ggagaataca cgctctgggg
     2341 gaacgagctg gagaaatacc gcaccagaat ctttgagcgt gttgatttaa tgcgtgacag
     2401 gagtggttat atctgtgacc gaagcgaaat cggtcaacat ccaccactgc aacgtgttta
     2461 ttcctctgcg acctttgagc aacttctgac gcaggtttgc cagacatggc cgcaatatac
     2521 ccgcaatctc cgccagccga agacatggcc ggagtctttc tgtcttggag aagaccgcca
     2581 gccagccatg ccatcactgg cagcaaggaa ggtggatttt acgcagggac gtctgctacc
     2641 gacgctgatg ccggtaatga gttctgtcga ccgggaaacc cgccagcttc agcttttgct
     2701 ggtcatgggc gtggatgact ctctgggcgg tgttgtccgg cttaatggta cgctgtatcc
     2761 cgcgtttgcc gttccctcag ccgataacag ccagctggtc atcagtgcac tgaccgataa
     2821 ggggttacgg tatgcagggt atggtgttgc cgtaaatcac gatgcagaca gccacatatc
     2881 acctgcgccg gaacttatgg agtttcatct gaaaacccgt gaggcaccgc tttttgctgc
     2941 cgtcaacaca ccagaaaaac agccggatca tctgttccgc tctctgggtt tcaatcgtac
     3001 ctgggatgaa tggcggcgcg aggaagatgc aagaacacac accaccgaga ggagacatga
     3061 tcgcggttgg tcacagtgaa tgtgaacaat attttgtttt gtttaacatc gtgttatgtt
     3121 cgtcgatgtt cttctatgtt catcgacagg gatgaatgca agaagcacac acataatgca
     3181 agcatagttt tgtttttccg cccccaaaaa ttgggggttc ttttttcgct gcattagagc
     3241 gcttttgacg caatttttca cttcaatagt accttcgcac tactaacaca aaaaaaacgc
     3301 agcagggagc gttttaggtg ccttaaaatc gattttagat cgtcatcttg atctgtttgt
     3361 cttccttgaa gcgaaaatgc agggctacaa ccgtgcgccc ctttttgatc gtctcaaccg
     3421 ttacagccag gtcgcttttt ttgttcagtt cgtcaacaca gggttttatt agtattttat
     3481 ttaagtctct gaattgtttg tatttatttt caatccctaa cattgaacga aagtcattaa
     3541 gagcaataat tctctctccc gtactgcgga attgctggag gaactcgtat attcttatac
     3601 tgtaactact tgataaacct gatatgtttt ttataacaac gcgcgtgaat tgccccttca
     3661 gttgcgtcag ataaggcatt accgcgtcag aaaaggttat tcgagccttg gcctcacctt
     3721 tagcgtactg tgcccgatac tggatccagc ggaactcctc acgcttctcg tcattcttga
     3781 gaatgatcga cctgtcccat aaacgatcga tcgcttcctg taactgaacc tcggcatttt
     3841 tccgccccat gtcagggaat gaatcaaaat attccgcagc ggtaatggtc attgtctttt
     3901 gcaattcagc atcagcacca gatttcaatc gactgataca cagcaacaga aaacgttgtt
     3961 cctgtagggt caacttgtag ctggcctcaa ccaaagcatt cgacttgtag gcaatcaagt
     4021 ccgacataca aagatcccgt tcaaatgtgg tgatgcttta atgtgcccca ca
//
