LOCUS       CP056633                7462 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00303 plasmid pRHBSTW-00303_2,
            complete sequence.
ACCESSION   CP056633
VERSION     CP056633.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148565
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00303
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 93.1772218741499x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 23:54:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,679
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,469
            CDSs (with protein)               :: 4,469
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 42 of 92
            Pseudo Genes (incomplete)         :: 51 of 92
            Pseudo Genes (internal stop)      :: 12 of 92
            Pseudo Genes (multiple problems)  :: 10 of 92
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00303"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00303_2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6227..7462,1..423)
                     /locus_tag="HV148_23350"
     CDS             join(6227..7462,1..423)
                     /locus_tag="HV148_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="QLW81875.1"
                     /translation="MKIVIVGGVAGGASAAARARRLSEDVSIVVFERGSDVSFANCGLP
                     YHIGGKIPLRQSLILKTPEDFKSRFNIDVRIRHEVMSVDPVNKMVTVKNLTSGEVYTEE
                     WDRLLLSTGAAPVVPPLPGLQEAGVFTLRNLTDMDAIQAWIEQHNIAHTTLVGGGFIGL
                     EVMEALSERGISVTLLEMGEQVMAPVDPEMASALHQEIRSHGVDLRLRTALTEVLRTET
                     GFRVALSDGGFLQTDMVILAIGVKPESSLATGAGLAVGKRGGISVNACMQTSIPDIYAV
                     GDAVETPDLVFQEPANVPLAGPANRQGRIAADNMLDRHSLYHGSQGTSICKVFSLSIGS
                     VGANEKQLKAHGTRYEKVYVHAADHASYYPGATMISLKLLFSPDTGKILGAQASGKKGV
                     DKRIDVLAVVQRAGLTVNDLEHLELTYAPPFNSARDVVNQAGMVAANVIKGDTVICHVR
                     DVLQREPGSYCLLDIRSPAELKQFGEYPDALSIPLDSLRENLDKLPEDKEIIIGCQSGL
                     RGHVAYRMLQSHGFRTYNLSGGFITWQAVTESMSQ"
                     /name="FAD-dependent oxidoreductase"
     gene            1734..2669
                     /locus_tag="HV148_23355"
     CDS             1734..2669
                     /locus_tag="HV148_23355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLW81876.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(2863..3261)
                     /locus_tag="HV148_23360"
     CDS             complement(2863..3261)
                     /locus_tag="HV148_23360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLW81877.1"
                     /translation="MRISNVEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQTIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQELKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            3438..4364
                     /locus_tag="HV148_23365"
     CDS             3438..4364
                     /locus_tag="HV148_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QLW81878.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDGVSRENRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            4367..4900
                     /locus_tag="HV148_23370"
     CDS             4367..4900
                     /locus_tag="HV148_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLW81879.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAIEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(4954..5175)
                     /locus_tag="HV148_23375"
     CDS             complement(4954..5175)
                     /locus_tag="HV148_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLW81880.1"
                     /translation="MTKLDENIPHIIDAAVDFMASSQAFREYLKKLPPDNTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESYWKKR"
                     /name="hypothetical protein"
     gene            complement(5719..6066)
                     /locus_tag="HV148_23380"
     CDS             complement(5719..6066)
                     /locus_tag="HV148_23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="QLW81881.1"
                     /translation="MPMNEDTKRQTDMKNAALGAADVLRGLANSDRLLLMCQLSQGEAN
                     VTELEAAVGITQPTLSQQLGVMRRLKLVETRRAGKMVYYRIGDNRIMTLLNTLYDLYCP
                     QADPDKVKKQP"
                     /name="helix-turn-helix transcriptional regulator"
     Typing          1735..2669
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.902"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3439..4361
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggcttaccg ttaacgatct cgagcatctg gagctgacct atgctccgcc tttcaacagt
       61 gcaagggacg tcgttaacca ggctggaatg gtggcggcaa atgttatcaa gggcgatacg
      121 gtcatctgtc atgtccgcga tgtgttacaa cgggaaccgg gcagttattg tttacttgat
      181 attcgttctc ctgcagaact gaaacaattt ggtgaatatc ctgacgcgct atccatccct
      241 cttgattccc tgcgggagaa tcttgataag ttgcctgaag ataaagagat tattatcggg
      301 tgtcagagtg gtctgcgggg gcatgtggca taccgtatgc ttcagtctca cggcttccgg
      361 acatataatc tgagtggtgg ctttataact tggcaggcgg ttacggaatc aatgagtcaa
      421 taagagatga ggtgccatac acatggcacc tcatcttcca gattcattga aaacgtatta
      481 tatggaatag aaatctacaa aaataattaa caccctggat ggtaccctat tgcgataatg
      541 tggactgttc aaaaaacagg cttaccgtac aataattctc tatatccaaa ctgaccccaa
      601 tgttcctgaa tgtaaatctc cgtacaaaga tatcgttgag ttttatataa ttcattgttt
      661 ttattgtgtt ttgttttttt gttgtgctgg tttgtagtta taaaaaaccc ctgggaatcc
      721 aggggtaggt aaggaaaaca aatgatactc agttagtgtt gcactttaaa cgggttgaag
      781 tataactcgt attaaatgag atgttgaaaa taagtgaaaa ccattgctgg ttgcaatagg
      841 gcagtatgcg taaaaaataa atttgtgatg cttttcaaaa aaacaattaa tacatgaata
      901 ttacagctca tatattacag tttataaaaa aactgtgttt tatctattac atagagaaaa
      961 attcttatcc tgagctgtga acttggttgt ttcgtttgat atatatcttt gtaaatgtgg
     1021 tttattatgc gggcattttt tattgagtat tgaatcagaa taagtgatgc agctaaattt
     1081 atttgcgtaa ctatttcatt gtcaatattc tgactcaatc tgacacctat cgtttctctt
     1141 ggattcccca cttttgtggt ttttttatcc ggaacgggat agtgccagtt gttttttatc
     1201 agcctcaggg tattttgggg cggttgcgaa cattgacgca ttccacaact aaaaaaattt
     1261 ctcacagact ctgattcttg ccccgaccct tggtcggttt ttttttgtcc tggcgaaacg
     1321 tgaagctctg aaacagaaaa tctccgggca gaaccttcca tccgggggga gaaaactttt
     1381 cgttttgagc cgttcggtgt tcagaacgca cgaaaccgat cgcacatcgc tttcgtgcat
     1441 agttatgcaa cgccctgaaa acgattctga cgcgtttttc tggttttgcc tggtgttttc
     1501 ttgtcttttt gcgttttttg cgccagaaag cgactgaggg cgttttaagg ggggcgtaca
     1561 acgggattta tggtaaatgg atcggttttt cgggaaggat cgacaggatt tgccgttggg
     1621 tgtagtgtaa gcgactgaaa aacaaacgcc cctgaaattc gtgtctatcc gccaagactt
     1681 tcacgaaaat acaggggcga tgtcggtcga taacctggaa ggaatcgtaa cacatgagcg
     1741 ccgcgcttca atacttcgaa gaaaatttac cccaccgtcc ctatcacacg gatgatctcg
     1801 ctttcggcct tcgtatctcc ggcaaagggc gcgcgcttct tgcacggtat atccagcaga
     1861 accagcctca tgcacagttc tggctggttt ttgacgttga ccgcgagggg gctgcgattg
     1921 actggagcga ccggaacgct cccgcgccga acatcaccgt taaaaatcct gtgaacggac
     1981 acgctcacct gctctacgca ctcaacatcg ccgtgagaac cgcgcctgat gcctcggtta
     2041 aggcgctgaa atatgccgca gcggttgaac gttcgctgtg tgagaaactt tgtgcggatg
     2101 tgaactacag cggcctgatt tgcaaaaatc cgttccacct ggaatggctg gtgatggagt
     2161 ggcgcgagga agcctatacc ctcgatgaac tggctgatta tctcgatttg agcgcctcag
     2221 cgcgtcgtag catcgataaa cattacggga tggggcgaaa ctgccacctg ttcgaaatga
     2281 cgcgcaaatg ggcttacagg gcgattcgtc agggctggcc agcattctca cagtggcttg
     2341 atgccgtgat tcagcgtgtc gaaatgtaca acgcatcgct tcccgttccg ctttcacctc
     2401 ctgaatgtcg ggctattggc aagagtattg cgaaatacac gcacaggaac ttcacgccgg
     2461 aaactttcgc acagtatgtg gctgatacgc acacgccaga aatacaggcc aagagaggca
     2521 ggaaaggtgg catcgctaaa ggcgaagcct acgatgacaa gcgtttcatg gcgctatgta
     2581 tgctggagaa tggatattct cagaaagcta ttgcggcgat gttggaggtt tctactcgaa
     2641 ccattcgaaa ctggaaaagc ggaaaatagc ctatatcaga taacagcgcc tttctggcgt
     2701 tcttttgagc agcaggtctt ttgccgtttt tccccgttca gccctgcaat ggtgctgttt
     2761 tccgcatgat tgtgctggta gtgctcgccg cagtctcatg accgagcgta gcgagcgaat
     2821 gagcgaggaa gcaccaggga accggaacac ggatggaggc acttacgcgt ctgggtttgg
     2881 ggatgtctcc agatcgtact taatttcctt aagttcctgt aatatcctgt cttttttata
     2941 gcatagctcg cctgtttttg tgtctaccag tcctgcttcg atcagtagcc cagccgcgag
     3001 aaagagtcgg tgattgcgtt cccgtcgttg tttttttccc tcgattcgtt tctggatcgt
     3061 ctgttttcgc tcgttctcct gtgcctgtaa gtcgtttttt tccgctgttg ccagcacgtg
     3121 gagaagtttg cgttcctgtt ctgtgagtcc ggcttcgttg tcgagcaggt caataatctg
     3181 tctctgtcgc gcggtctgtt ctccgagctt cctgatgaat ccgatccgtt tcttcagcca
     3241 ttcgacgttg ctgatgcgca tgtttgtggt actccctggt gtttttttac ggcggtttgt
     3301 ggtcgtgcag tatatcgctt gcggtcggcg ggtgcagggg gtatcttccg tgctaattcg
     3361 aataattaga aggaagcgca cttatatgtt tctctccttc gtcgagaaac agtgcgctcc
     3421 tgcggagggc tgaaaccgtg gcaacctatc acctcagcgt taaatttggt ggcaaggggc
     3481 aggctgcaaa ccacgccgat tacatcgagc gcaaagagaa ataccgcgac cgtcaggatt
     3541 tggaatattc cgcacacgga aacatgccgg aatgggccag ggataatcct tcccatttct
     3601 ggcaggcggc tgaccagttt gaacgtgcga acggttcgac ctatagggaa cttgaaattg
     3661 cattaccccg tgaactgaca ccggaacagc gccttgaact ggtgcaggat tttgtacgtc
     3721 aggaagccgg cgaacggcac gcctggagtt ttgccattca caatccgaaa gcgagcattg
     3781 acggcggcga acagcctcat gcgcatatca tgatgagtca gcgtgtgaat gatggcattg
     3841 accgtacacc ggaacagtat ttccggcgct ataacgccag gtatcccgag cggggaggtg
     3901 cgaaaaagga ttccggaagc ctgactccca ctcagcaaaa agagcagttg cgggaattgc
     3961 gaaaacgctg ggaggtgaag cacaacgaac atatgagaaa gcatggcttt gaaaggggct
     4021 tcattgactg ccggacactg aaagaacagg gcattgaacg acgtcccgaa gtgcatctgg
     4081 gttttgaagc tgccggacga cttgatgatg cccagcgtca tgacatcatg caaaaacggt
     4141 ctgaaccgga ctattcccgc catcttgaac aggcgcgaat ggtcgggcat gacatcaggg
     4201 aatggctgga tacaccggtt aagcaacatg aactggtcag aaggctgcaa catgatgttg
     4261 cgcgggagca tggctttgac atgagagacg gagtgagcag agagaaccga actgaacctg
     4321 aacgggttaa atcaccgtca cgtggatttt ctatgggatt ctgaacatgg ccagcacaga
     4381 agaaaaatta ctcacgctcc tgttatcggt ggagcatctg caacataccg caatagagca
     4441 acagaaagct gttgctgatt ctctggctgt ggcaagggaa actcttcgcg gatatcggga
     4501 tatttctgac ggtgtggcaa aacaggtcag ggatgaagtc agaaacgagg tgcagaagct
     4561 ggacgtggca ggactgatag gtgagcgcat cagccaggat tttcagcggc tggaagagtc
     4621 agtcgcaaac atgaaggcaa acgcggatgc cctgaacagg gatattgcga caattcacag
     4681 caatatcgtg caggaataca acacccttaa agggtatagc tggaaatggc tgggtggggt
     4741 gtcagtggct tttctggtta tgttctgtgt gctcggatgg ggaatgaaaa caatactcga
     4801 caggaactac aacgccacag cggcggtgta tcagaaagtt gtgacactgg aagataaact
     4861 ggtggagaaa cctgtcacgg ggaagagccg gaaaaactga cgcgcttcgc ttgtccaacg
     4921 cctcacggag cgcgaaagga tttcgcgaac cgttcaccgt tttttccaat atgattctgt
     4981 ctgctttggt cgataaagca gtcgatagta ttccagcctt cgtatatata acggtacgtg
     5041 ctcatcagta agatctggcg gtattgtgtt atcagggggg agttttttca gatactcacg
     5101 aaatgcctgg cttgatgcca taaaatctac agctgcatcg ataatatgtg ggatattttc
     5161 atctaatttt gtcatgatga ttgatctgat attatcacgg ttcgtgaatt atagtatgtc
     5221 actattattt atgcgttagt gaaccagaaa aaatcccccg ttggtatagg gggatatgta
     5281 aggagaaata acttcgcagg tgttcttgtt tgtattataa cgtgaaaaga atgcacgcaa
     5341 cttaattaat ctgccgtact tcctttcagt tttcaagata aggtttataa taaaacatgg
     5401 aatggaagca agctttaagg tttcagctct aaaattttta atatctatta actagctagt
     5461 tgttgtcctt gtgagcatgt tgtatataaa agctaggata atatcctgac cggagtattc
     5521 atattcctta tcccctctgt gttctcagag gtttttttct gtatttaatg atatgagctg
     5581 gaatgcgatt ccggcccccc cccgtttaca taattctccc tgacatacgg caccggaatc
     5641 gcattgattt tgcattgtta atgtctaact gtgctggtga atgttggggg cagtttggat
     5701 atcgaaaatt attgtacgtt aaggttgttt tttgaccttg tcggggtccg cctgcgggca
     5761 gtagagatca tataaggtat tcaataacgt catgatgcgg ttatccccga tacggtaata
     5821 taccatttta cctgcccgtc gggtctcgac cagcttcagg cgacgcatga cacccaactg
     5881 ctgagacagg gtcggctgag tgataccgac cgccgcttcc agctcggtca cattcgcttc
     5941 cccctggctt aactgacaca tcaacaatag ccggtcgctg ttggccagtc cccgtaggac
     6001 atctgctgcc cccagggctg catttttcat atcagtctga cgttttgtgt cttcattcat
     6061 gggcattgct ggtttcttat attatgtatt tttatataat attgttcagt ataatgatag
     6121 tggcgtaatt ttgccaccgt gatcttcagg tctgcagcgt ccagctggac cgggtaacta
     6181 attatatatg gcagatattt tgccccgagt gaacagagag gatgtaatga aaatagtgat
     6241 tgttggtggt gttgccgggg gagcatccgc tgcagccagg gctcgtcggt tatcagagga
     6301 tgtcagtatt gttgtgtttg agcggggaag cgatgtctct ttcgccaact gtggattgcc
     6361 ttatcatata ggcggaaaaa ttccgttacg gcagtcgctg attctgaaaa ccccggagga
     6421 ttttaaatcc cgttttaaca tagacgtccg tatccgtcat gaagtgatgt cagtagaccc
     6481 ggttaataag atggtgacgg ttaaaaatct gacctccggg gaggtctata ccgaagaatg
     6541 ggatcgtctg ctcctcagta ccggtgccgc tccggttgtt ccccccttgc ccggactaca
     6601 ggaagcgggt gtctttacac tgcgaaatct tactgacatg gatgctatcc aggcgtggat
     6661 tgagcaacat aatatcgctc acaccacgct tgtcgggggg ggatttatcg ggctcgaagt
     6721 gatggaggca ctgagcgaaa gggggatcag tgtgaccctg ctggagatgg gggaacaggt
     6781 tatggctccg gttgatcctg aaatggcatc ggccctgcat caggaaatca ggagtcatgg
     6841 cgtggatctg cgtctcagaa cggcgctcac cgaggtactg cggacagaga cgggattccg
     6901 cgttgcactg tctgacggtg ggtttctgca gaccgacatg gttattctcg ccatcggggt
     6961 aaaacctgag agcagccttg ccacaggagc cgggctcgcc gtgggtaaac ggggaggcat
     7021 aagtgtcaat gcctgtatgc agacaagtat ccctgacatc tatgccgtgg gggatgcagt
     7081 tgaaacacct gaccttgtct ttcaggaacc cgctaatgtt cctctggcag ggccagccaa
     7141 ccgccaggga cgcattgctg ctgataatat gctcgatcgt cacagccttt accatggcag
     7201 tcaggggaca tccatttgta aggtattttc gctgagtatt ggcagtgtcg gggcgaatga
     7261 aaagcagctg aaagcccatg gtacccgata cgagaaggtt tacgtccatg ccgctgatca
     7321 tgccagttac tacccggggg caacaatgat cagtctgaaa ctgctgttca gccccgatac
     7381 cggtaaaatt ctcggggccc aggcgtcagg gaaaaaaggg gttgataaac gcatcgatgt
     7441 cctggctgtc gtacagcgcg cg
//
