LOCUS       CP056631                1610 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00304 plasmid pRHBSTW-00304_7,
            complete sequence.
ACCESSION   CP056631
VERSION     CP056631.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148566
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00304
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 105.394030148214x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 00:08:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,137
            CDSs (total)                      :: 5,022
            Genes (coding)                    :: 4,738
            CDSs (with protein)               :: 4,738
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 94 of 284
            Pseudo Genes (incomplete)         :: 171 of 284
            Pseudo Genes (internal stop)      :: 45 of 284
            Pseudo Genes (multiple problems)  :: 24 of 284
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00304"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00304_7"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(1187..1610,1..86)
                     /locus_tag="HV149_25685"
     CDS             join(1187..1610,1..86)
                     /locus_tag="HV149_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QLU22372.1"
                     /translation="MIVASEHQAKLRQKEIQEIDRKASKEHEEAKKNSNFTQTYPKGWQ
                     RIRELLRDKQGAAAIGLYSFFAEHIDPTCGAVVCDQQFLANQMGVSTRTIRRWLDYLEE
                     KGALVRIPVAGKVCAYALDPHEVWKGYNTSKDYSAFVTKTLVNKDGEIKRRIMAMFSVE
                     ESGQEQ"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 ccaagacatt agtgaacaag gatggagaaa taaaacgtcg aataatggct atgttttccg
       61 tagaggagag cggtcaagag cagtaacgaa gcgactcttg acggatcgga acgaccacca
      121 ccctcggaaa gggggatggt tcgccacagc gaactatccc catgataatc cgccatttaa
      181 atagtaggga gcgattatat accaccaccc ggcaaaggta tcttaattta taaaataaac
      241 aaaacaatca aaaacaaaca cttaaaataa aaaacaagct acgataaaat atatttatat
      301 attttcatta aatatatttg attttcctat tttttaaata tatacattcc attacaacaa
      361 taaaataata ataaaaatag gagaataaaa tgaatataaa aataaaaata aacagattat
      421 gcttaatatt aatatgtgta gctttgttac gcggtgatag taataatgaa aaaccacaga
      481 cgcactatct ggcgccggtt aatataataa aaaaacaaaa accgcttacc aagtgcaagg
      541 cggcgccacg cgccacattg caccagaggt agtttaccca acagtgcgcc gtcaatgata
      601 aataaactcg caagctcgtc attgacggta tcaagccagc atcagtgaat cagtagtgcc
      661 cgccgcagcc tcacggcagg agcgtagcga ccgagtgagc gaggaggcac cagatcatca
      721 gaacttgcag gttcttctta cgctctgagg ctaaatacaa catagccagt atacactccg
      781 ctagcggagt gactggttca gggcttcgcc ccgaaacccc tgaaaaccgt cagtcatggt
      841 gatggctcac tatgcgagcc atcaccagat acaaggttaa atgggcattt tgatcgtcaa
      901 ggtcaagccg ctaacgcggt cgcctatggc gaaccatgac cctcaaaatg cccgaccgga
      961 gggagggagc taccttgaac tttggtgttg tttgttggtt ttcatagaag agaatagaga
     1021 taaataataa gttattgatt tttatagttt tgattttttc tgataggaca ccagctgtgt
     1081 ccgcacgtgc ggacaccagc tgtgtccgca cgtggctgga cataggacac cacaagtcct
     1141 atgatgggac aaaaatgtcc tataaaaaag acaccgaggg ataaagatga tcgtagcttc
     1201 agaacaccaa gcaaagctca ggcagaaaga aatacaggaa atcgacagga aggcatctaa
     1261 agagcacgag gaggcgaaga agaacagtaa cttcactcag acatacccaa agggctggca
     1321 acgcattaga gagcttttga gggacaaaca gggtgctgcc gctataggtc tttactcatt
     1381 tttcgctgaa catatagatc ctacatgcgg tgccgtggtc tgcgaccagc agtttttagc
     1441 taatcagatg ggtgtttcta ctcggacaat tcgtcgttgg ttagattatt tggaggagaa
     1501 aggtgctttg gtaagaatac cagttgctgg aaaggtttgt gcttacgctt tagatcctca
     1561 cgaagtctgg aagggatata acacatcaaa agactactca gcctttgtta
//
