LOCUS       CP056630                1917 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00304 plasmid pRHBSTW-00304_6,
            complete sequence.
ACCESSION   CP056630
VERSION     CP056630.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148566
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00304
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 105.394030148214x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 00:08:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,137
            CDSs (total)                      :: 5,022
            Genes (coding)                    :: 4,738
            CDSs (with protein)               :: 4,738
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 94 of 284
            Pseudo Genes (incomplete)         :: 171 of 284
            Pseudo Genes (internal stop)      :: 45 of 284
            Pseudo Genes (multiple problems)  :: 24 of 284
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00304"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00304_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(1755..1917,1..74))
                     /locus_tag="HV149_25675"
     CDS             complement(join(1755..1917,1..74))
                     /locus_tag="HV149_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLU22371.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            1193..1495
                     /locus_tag="HV149_25680"
     CDS             1193..1495
                     /locus_tag="HV149_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="QLU22370.1"
                     /translation="MSSMVYCRGCGKEIHETAKSCPHCGATNASSGSGEKSRIAAALLA
                     FFLGGFGAHKFYLGKIGQGFLYLIFCWTFIPAIIAFIEFIIYLCDSDEKFARKYG"
                     /name="TM2 domain-containing protein"
     Typing          complement(240..413)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          392..883
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 gctctttcag ctgcggcaac agcatcatca agaaattttt tctctgcagg cgtcaagttt
       61 gtcagtctgg ccataacgtt ctccagtcag aataaatcag cacagtctgc cagacacagt
      121 agaaacgatg aagcgccagc gacggtgaaa ggcagtcaga aacggtgaac gggagcggat
      181 tttaatccgg tgccgtgagg cgttggacaa gccgcaggcg cgtcattagc ttttagcggg
      241 tttcggggcg cagccctgaa ccagtcacgt agcgctagcg gagtgtatac tggctgacta
      301 tgttggcacg gatgtggaaa tcagtgaagt gcttcatgta gcaggagaaa aaaggcggca
      361 ccagcgcgtc agcaggatat gtagtacagg atatattccg cttcctcgct cactgactcg
      421 ctgcgctcgg tcgttcggct gcggcgagcg gtgccggctt acaggcgggg cggaaatttc
      481 ctgggagata ccaggaagga gcttaacagg gaagagataa ggccgcggcg aagccgtttt
      541 tccataggct ccgcccccct gacaaacatc atgaaatctg acgctcaaat cagtggtggc
      601 gaaacccgat gcggcttaaa gataccaggc gtttccccct ggtggctccc tcgtgctccc
      661 ccgcattcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttatctca
      721 ttccacgcct gacactcagt tccgcgaagg cagttcgctc ctggatggac tgtatgcacg
      781 aaccccccgt tcagtccgac tgctgcgcct tatccggtaa ctatcatctt gagtccaacc
      841 cggaaagaca cgacttatcg ccactggcag cagccactgg taactgattt agaggagtta
      901 gtcttgaagt tatgcgcctg ttaaggctaa actgaaagaa cagatttggt tactgcgctc
      961 ctcgtaagcc agttaccacg gttcaaagag ttgctgctct gagaaccttc gaaaaaccgc
     1021 cttgcagggc ggttttttcg tttacagagc aagagattac gacgccacag aaaggatctc
     1081 aagaagatcc ttcaccgtaa tggaaatcta tccaatccga gcacatagga tacacatcgt
     1141 tataaaacat cggatattat gatcccaatt aaaataagct cgaggataaa aaatgagttc
     1201 aatggtttac tgccgaggat gtggcaagga aatacacgaa acagcaaagt catgcccgca
     1261 ctgcggagcc acaaacgcct cttcaggttc aggtgaaaaa agtagaatcg cagccgcgct
     1321 attagcattc ttccttggtg gatttggcgc acataaattt tacttaggaa aaataggaca
     1381 ggggttcctg tatctaattt tttgctggac attcataccc gcgatcatcg cgtttattga
     1441 gtttattatt tatctatgcg attccgatga aaaattcgca cgaaaatatg gctaactcat
     1501 agcaaaaaaa agccccctaa gggctttttt ttgtacctga cggtcagacc gattaaacat
     1561 aacatccctt gcccgcaccc ccgcaggcgc cctcagcgca atcctgctgc atcagggccg
     1621 tttccagcac ctttcctcac cagtatcccg gaaaaacgcc cctgatgatt tctgcgcgac
     1681 tgcatggcta tgcacgcagg tgaatggcag gtgatttcgc agcgtcagca ccactaaacc
     1741 gccaaaagcc aaaatcagcg gcgcggcgcg cgcggacgtg accaggcaaa atcggactgc
     1801 tgccgctcat cctcacgtaa tgcacgctgg cggtcggcgt aacgctgcaa ctcaatttgc
     1861 gcacgcgctc tgttcaggac gatgtgccgg ttaggctgat ttagcttttt acctgac
//
