LOCUS       CP056617                1565 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00317 plasmid pRHBSTW-00317_5,
            complete sequence.
ACCESSION   CP056617
VERSION     CP056617.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148573
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00317
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 90.2123762706635x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 23:39:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,445
            CDSs (total)                      :: 5,322
            Genes (coding)                    :: 5,106
            CDSs (with protein)               :: 5,106
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 95 of 216
            Pseudo Genes (incomplete)         :: 117 of 216
            Pseudo Genes (internal stop)      :: 44 of 216
            Pseudo Genes (multiple problems)  :: 36 of 216
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00317"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00317_5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(687..1166)
                     /locus_tag="HV156_27225"
     CDS             complement(687..1166)
                     /locus_tag="HV156_27225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QLV23322.1"
                     /translation="MYIGTEEQRKKRLKELEKLNKESESDPKNNGPFTQVYQKGWERIR
                     ELSKDKQGVVAIPLYSFLAEHIDPSCGAVVADQQFLADKLGVSRSTIKRWLNYLESKNA
                     LVRIPVAGKVCAYALDPHEVWKGYNTSKNYAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          177..437
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.275"
                     /coverage="100.0"
                     /evalue="1.83e-109"
ORIGIN
        1 atgtgcaaat gtaataccgt cacccgcgac gatattccgt ttgccatctg gtcagggctt
       61 cgccccgaca ccccgcatca gtgcgtacgt gctcaatgtg gtttccggtt ctcttccgct
      121 tcctcgccca ctcgctcact aacgttcggt cgctggggct gcggcgagcg gtgccacctc
      181 tcgcatgttg tcggtgattc cgctgttgtt ctttcgggga taagtcgtca gatagccctt
      241 caaacgcgct cagaagctct cagacgcacg aaacggggtt taaatgcccg tcagggcatg
      301 gaaggcgcga aaaacgctta cagagcgttt caggaggtca ggcgcgccga tacccctagc
      361 caggggcttt caggtcgcca ggaaccgcgc cagccccctg caaagcccat ttgcacggta
      421 caggcagcca ctcggttggt aggtggtcgt agaccgtcgg tgttacagcg atgggggtct
      481 ggggttctcc ccagacggtt tccggttgcg ctcaaggcgc tgccggaacc gtggcggctc
      541 gatcgttctg gagatcgggg agcaaaaaca cgcaagggat tttgaggcgg caaaagagca
      601 atgcaggata gacaacccga tgtcaaatgt taacatttga atcagattgt ccaaaaacgg
      661 ggtttttgcc tgtgcctgcc gctggctcaa tttgaaaaca tggccatgat tcggcgctga
      721 atatcaccgt ctttgttgac cagtgtttta gtgacaaacg ccgcgtagtt tttagatgtg
      781 ttgtatccct tccagacttc atgtggatcg agtgcatacg cacaaacctt accagcaaca
      841 ggaattctga ctaacgcgtt ttttgattct aagtagttga gccagcgctt aattgtgctt
      901 cggctaacac ccaatttatc tgctaaaaac tgctgatcgg caacaacagc accgcatgag
      961 ggatctatgt gctcagctaa aaacgagtaa agcggtattg ctacaactcc ctgcttgtcc
     1021 ttcgacagtt ctcggatcct ttcccagccc ttctgataaa cctgggtgaa tgggccgtta
     1081 tttttcggat ctgattccga ttctttgttt aatttttcca gttcttttag acgctttttt
     1141 cgctgctctt cagtcccgat atacatggta aatctccacg gtctaatgat ggctcaaata
     1201 taagccatca tagctcaaac gtaaaccatg ataggtcgac ctagctcaca catgcgtgag
     1261 ctaaccttag accacacatg cgtgagctaa cccataatgc aagtagctaa aaaatcaata
     1321 acttacaaaa tcgcccttaa atatcttttc tgctgaaagt gttgcgcaaa gcggaacact
     1381 tttttggggt gtcggggcga agccctgacc cagtcacgta gcgatagcgg agtgtatact
     1441 ggcttactcc tctgcaagcc ttaccagctg tgatttttgc cagatttcac gctctcaacg
     1501 ttcaacagct ccatcatggt tttgccgtcc gttcaccgct aaaacgcctc agaaatcggg
     1561 acagg
//
