LOCUS       CP056616                4072 bp    DNA     circular BCT 16-MAY-2022
DEFINITION  Escherichia coli strain RHBSTW-00317 plasmid pRHBSTW-00317_4,
            complete sequence.
ACCESSION   CP056616
VERSION     CP056616.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148573
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: WTP2 T1-00317
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 90.2123762706635x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/18/2020 23:39:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,445
            CDSs (total)                      :: 5,322
            Genes (coding)                    :: 5,106
            CDSs (with protein)               :: 5,106
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 95 of 216
            Pseudo Genes (incomplete)         :: 117 of 216
            Pseudo Genes (internal stop)      :: 44 of 216
            Pseudo Genes (multiple problems)  :: 36 of 216
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHBSTW-00317"
                     /isolation_source="Wastewater influent sample"
                     /db_xref="taxon:562"
                     /plasmid="pRHBSTW-00317_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            349..1041
                     /locus_tag="HV156_27210"
     CDS             349..1041
                     /locus_tag="HV156_27210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QLV23319.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1297..3456)
                     /locus_tag="HV156_27215"
     CDS             complement(1297..3456)
                     /locus_tag="HV156_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QLV23320.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            3635..3889
                     /locus_tag="HV156_27220"
     CDS             3635..3889
                     /locus_tag="HV156_27220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QLV23321.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     Typing          complement(3099..3457)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          3458..3634
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          350..1041
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1301..3543)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attctgcttt cttgcaacac attgttttta ttggtttttt gttttcagcg ccccaagtta
       61 tccacaggct cgccgttaag tttttagatc ttgtttttaa gatcttgttt ttaatacttg
      121 ttttagggtc aaaattcatt aataaaatca atatgttata gaggtatatg tggaggaatt
      181 taaggtaaag gtggaggaat ttaaggtgtt taggtggagg aatttaaggt aaaggtggag
      241 gaatttaagg tgttattcac agaaatgtga ggatataaaa aaaaatattc cacatttctt
      301 atttgtgggg cacattaaag catcaccaca tttgaacggg atctttgtat gtcggacttg
      361 attgcctaca agtcgaatgc tttggttgag gccagctaca agttgaccct acaggaacaa
      421 cgttttctgt tgctgtgtat cagtcgattg aaatctggtg ctgatgctga attgcaaaag
      481 acaatgacca ttaccgctgc ggaatatttt gattcattcc ctgacatggg gcggaaaaat
      541 gccgaggttc agttacagga agcgatcgat cgtttatggg acaggtcgat cattctcaag
      601 aatgacgaga agcgtgagga gttccgctgg atccagtatc gggcacagta cgctaaaggt
      661 gaggccaagg ctcgaataac cttttctgac gcggtaatgc cttatctgac gcaactgaag
      721 gggcaattca cgcgcgttgt tataaaaaac atatcaggtt tatcaagtag ttacagtata
      781 agaatatacg agttcctcca gcaattccgc agtacgggag agagaattat tgctcttaat
      841 gactttcgtt caatgttagg gattgaaaat aaatacaaac aattcagaga cttaaataaa
      901 atactaataa aaccctgtgt tgacgaactg aacaaaaaaa gcgacctggc tgtaacggtt
      961 gagacgatca aaaaggggcg cacggttgta gccctgcatt ttcgcttcaa ggaagacaaa
     1021 cagatcaaga tgacgatcta aaatcgattt taaggcacct aaaacgctcc ctgctgcgtt
     1081 ttttttgtgt tagtagtgcg aaggtactat tgaagtgaaa aattgcgtca aaagcgctct
     1141 aatgcagcga aaaaagaacc cccaattttt gggggcggaa aaacaaaact atgcttgcat
     1201 tatgtgtgtg cttcttgcat tcatccctgt cgatgaacat agaagaacat cgacgaacat
     1261 aacacgatgt taaacaaaac aaaatattgt tcacattcac tgtgaccaac cgcgatcatg
     1321 tctcctctcg gtggtgtgtg ttcttgcatc ttcctcgcgc cgccattcat cccaggtacg
     1381 attgaaaccc agagagcgga acagatgatc cggctgtttt tctggtgtgt tgacggcagc
     1441 aaaaagcggt gcctcacggg ttttcagatg aaactccata agttccggcg caggtgatat
     1501 gtggctgtct gcatcgtgat ttacggcaac accataccct gcataccgta accccttatc
     1561 ggtcagtgca ctgatgacca gctggctgtt atcggctgag ggaacggcaa acgcgggata
     1621 cagcgtacca ttaagccgga caacaccgcc cagagagtca tccacgccca tgaccagcaa
     1681 aagctgaagc tggcgggttt cccggtcgac agaactcatt accggcatca gcgtcggtag
     1741 cagacgtccc tgcgtaaaat ccaccttcct tgctgccagt gatggcatgg ctggctggcg
     1801 gtcttctcca agacagaaag actccggcca tgtcttcggc tggcggagat tgcgggtata
     1861 ttgcggccat gtctggcaaa cctgcgtcag aagttgctca aaggtcgcag aggaataaac
     1921 acgttgcagt ggtggatgtt gaccgatttc gcttcggtca cagatataac cactcctgtc
     1981 acgcattaaa tcaacacgct caaagattct ggtgcggtat ttctccagct cgttccccca
     2041 gagcgtgtat tctccgttcc ggttgtgaag cagaaggtaa caggtcattt ccccgttacc
     2101 aggcatgggg gcctcgccat accccgtaac gactgcgctc agtacccctg aatccataac
     2161 ggcattgtca tttttcctga gctgttccgc tcgtcgctcc gcgcgactac gttctgtatc
     2221 ctcctgctcc ttccggcgca attcctcttc acgtttaagc aactggactc tgagagctgc
     2281 aaggccggat gccccccact gctgccactg tttttcaaat gccgtctgac tcgcgggaat
     2341 gtcctgtttc tccaccttcc ggtaatgctg tcgcaatttt tccatttgct ggctcagcga
     2401 ctggattttt ccctgttcgc tacggtattc cggtgccgcc cattgcatca ggggaatacg
     2461 ggaaaaaccg gacgaaaacg gcgatgattt tttcaggctg gcagcatggc tcatcgcttc
     2521 ctttcgttcg gtgttcagct cttcccagcg tctgcgcgtc gcctgcatat ctgccatcat
     2581 cgacatggca ttactggtga gtttccatgc cgcattactg tccttcggca acgcttcgat
     2641 gatgtcacgt agtgtgtacg aagttgcgtt gtcaggaagc acgatattca gaaggtcagc
     2701 cacacggcgg gcaggctctg tcagcccgct gttcctgagt tcagtgcggg aaatctgtat
     2761 ttccaggtta agcctgttaa tcagacgatt ctgttcgccg cgttctgttt cgataccgcg
     2821 tgtttccatc gcacaggcag actttccgag gtgaatacca ggcgttttct caagcccctg
     2881 tcgctcgtat gaacgatggt ctatctcttc ctgataaccg gcgttcgcca gtgctctgtt
     2941 tgcatggtca gcccatgatg cacgccacgt ctcggcatgt gtccggtcgt tccagtccct
     3001 gacctttccg ccgaacccag caggtccgac agccctcgtg gtcagcatga tgtgcgcatg
     3061 gggatttgtt ttatccatgt gatggaacgc cacatcagca atcatgccct gactgacaaa
     3121 gttttctctg acaaaggcga gaacggtttc ccgtgcggca tcctgcggca gttcacgggg
     3181 gatagcgagt tcaatttccc gcgcaagttg tgagttcttc cggcattcgg ccttctcaac
     3241 ggcattccag agttcggcac ggttcgtaca ccaggcaggg gctgtcgcgg gggtcagaat
     3301 cacagcttca gccacaccgc ttttgcgggt gtagtcgtga gtcagtccgg tacgctcatc
     3361 aagcatcagt gaaccggaac gataggcagc ggaagctacg gcgctgtagc cgctgcttct
     3421 ggagataatt ttcatgctga gatgataaat cgccatttct gccgtaatgc ctttttgtag
     3481 ttttctttca gccccgcagg gcgcacacaa tcgaaacgga gtggagattg tataagtgcg
     3541 cactttgtga tttgtcgcgc attatgccag ctcagttttt aaactaccag tgacgcgcta
     3601 aaatgcgatt tttcatcgcg cacggagtta catcatgagc agaacgctcg aacagaagat
     3661 tgctgatgct gaggcgagat tacagcgcct gaaggcgaag agccggagcc tggacacagc
     3721 gcagaaggtt gttgtgggtg cagcattgct ggcaaaggtc agaaagccgg aggaggtgca
     3781 gttgcgtgcc tggttactcc agttcctgaa ggcagaggtg acacgacagg ctgatgtgac
     3841 gcgcatactg ccgctgatta acgagctgga ggcgctgcca ggacagtgat tgtatcgtcc
     3901 gctcgccgca gccccagcga ccgagcgtta gcgagcgagt gggcgaggaa gcggaagtga
     3961 atccgtcgcc aggttgagca catacgcacg gtttttcggt aggtgatatg cgctgaaccc
     4021 gcatgaatac tggatttcca cagggttgga aggggagtat tcggaacttg ca
//
